LOCUS       Exported                6784 bp ds-DNA     circular SYN 15-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     *3173 bp promoter for flp-8 inserted into Han's pHW393 backbone
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    37..3209
                     /locus_tag="flp-8 promoter"
                     /label=flp-8 promoter
     misc_feature    37..57
                     /locus_tag="Forward Primer"
                     /label=Forward Primer
     misc_feature    3190..3209
                     /locus_tag="Reverse Primer"
                     /label=Reverse Primer
     misc_feature    3255..3296
                     /locus_tag="42bp intron"
                     /label=42bp intron
     misc_feature    3314..3337
                     /locus_tag="NLS"
                     /label=NLS
     misc_feature    3344..3784
                     /locus_tag="SK GAL4"
                     /label=SK GAL4
     misc_feature    3344..3346
                     /locus_tag="ATG start"
                     /label=ATG start
     misc_feature    3842..3874
                     /locus_tag="VP16 domain"
                     /label=VP16 domain
     misc_feature    3881..3913
                     /locus_tag="VP16 domain(1)"
                     /label=VP16 domain
                     /label=VP16 domain(1)
     misc_feature    3920..3952
                     /locus_tag="VP16 domain(2)"
                     /label=VP16 domain
                     /label=VP16 domain(2)
     misc_feature    3959..3991
                     /locus_tag="VP16 domain(3)"
                     /label=VP16 domain
                     /label=VP16 domain(3)
     misc_feature    4037..4469
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             4832..5491
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      5589..6271
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    6611..6633
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     6639..6659
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    6673..6723
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(6724..6784,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcat gcgcggccgc actgactggg ccggcccgtg atggagttcg cagcaatgag
       61 tgctcaaatg gagtctgcat gaaaatgatt ttccaggaaa aaagcggtga gatagatcaa
      121 aattaattgt ttattttatt ttctttttac ttattttagg aatcaatcat gtttggagaa
      181 gttgcatacc aaatgcaaaa gaacaaatcc gatcagactg tacaaaaatt actagtggag
      241 aaggtatgta atattcaaat tgttccatat aataaaaaaa aaatatatat ataactttca
      301 ggaaaccttg aagtttgcac atgcgatggt aatttatgca acaatgacgt caatttaaat
      361 ctaatactaa taattatgtt ttctgcagct gtgttattat tatgatgagt tgttcattaa
      421 acctacatat cggaatcaaa tcaaacttag atctctattc ttttcatttt ccatcggttt
      481 tatatagaac ttttgccgaa gtattttgat tgcataccaa ataaatgttt ttacgttgtt
      541 ggaaatgttt attaaaattt aaaggaacac agaatagcca aagtgttttt aatttccaaa
      601 ttttttttta ctcctcgaac agtacccttg aacttcagct cgctattttg aatattcctg
      661 tcacgaagtc ggtgaataca aagattcact cgctcacgac ttattaataa atctaaggat
      721 ttattaacaa atgattaaat gtaaagattg attcattgtt attacgtaac acttgctctt
      781 caacactcaa catcatctct tcaatcaacg gcaacaacca tgcagaagga cggcgtcaga
      841 aaccccgatt caaactaccg ccaaaaggac gattcaaggc gcatgaggtc tcgccacgac
      901 acatacaaca acgactgcga ccgtactctt gtcatcatgg cgcagcgcac attggcctat
      961 atcgtcagtc ttcatcggtc ttcaccaccg cagcacgtgc ccgacggtgg cacgtttaaa
     1021 tgaatgttga gtgttagtga ttcaaggttt tccccttgaa tcactcgtaa taaatgagtt
     1081 aattaaataa gagtcttttg ttagttccta tcagtacctc atgggccgga aaccactccg
     1141 aaacaattcc cataatttta ttgttttcca ttttccagga aaaaaaacag ttctttgaca
     1201 ttttcaagcc aaaaactatg tgtgttccgt cttaagacca tgcattggaa attacgttcg
     1261 tcactatttt tgtgtgtatg tgttttggat tcctcttctc acatttgcgg tcacaacagt
     1321 atcgttcatc ggtttttatg ttgtgataaa atagagaaaa agtaaaacga gcatattttt
     1381 gatagcggaa gaaaaatttt gagattacat ataacaaagc ggaaagtgat tatcaaatgt
     1441 caaattatca aataatttgg aagcttttca attcaatata cagggttgat tttcaatagg
     1501 tggtgcaagt aaaaaagaaa tactgtattt atatttaaag ataaatacat tttgtttaaa
     1561 cacgtaaata ttgtttcaag ctggccgcat atcaaaataa aaccgtacaa aaactttcca
     1621 gtttttctta attttcagtc aaggttttag taaaatagcg aggcgacgaa aatatataac
     1681 tttccgagaa gttcagagca gttaataatt attatcaact ttttacagaa tggtttacat
     1741 tttttcaaca ttaattatag ttaaaggaca atacattttt caaaatattc actgtaaata
     1801 tgaatgaata cttttggaaa ataatcaaaa agctgactga ctactgttaa acaatggcgg
     1861 agtgataaac tagcaaattc acttattatg cacataatta taatgcagga taagatgact
     1921 gactttcaag acattcacta tttaatgatt agacttccat ttttgaccat cccattttga
     1981 cataagaact ccttgatatt ttgttctctt tgaaaagtct tgttttactc tactttaaat
     2041 gaattcattt tcgtcatgga cgatctcttc aaaaagtatg tgggcacctt tactttgaat
     2101 acacgcaatc ttcaccgtag tttcatgttg ctcgaatgaa tattcaatga ttggagggca
     2161 agatttatga agagattgcc gtgaaatcat tttgcgaggc gaatcatcga ggaagcaact
     2221 gattcgtgat gaataatttg cgcggtggat aagtttttgg tttcaaagta cccccccccc
     2281 ccccccccga gaagaggtgg aaaaaagttg aagaattaga tgaaggagaa gtccaagtgt
     2341 tacgtcaaca atttacaaat gcatagagtt ggtataatta gtttcggagg gtgtcggtgg
     2401 ctaaatttat ctgttagtta cctcccagca tgaattccgt gtattcactg tgttcagtcc
     2461 ctcgactgct cggttataca gctgatagat agacgaggaa gaagaaaaca cacaaaaacg
     2521 taaagtgggc ctctttaaaa aaatttaaaa aacaaaataa ggacacaaaa atataaatat
     2581 gcattgtttt catttttcaa aacgagggta gcttcgagaa atttccattt tcaaatcaaa
     2641 cgaattgcta caacaatgtc aaaatgttaa aatttttctt ggtggtactg tatgactcta
     2701 aagtttgatc tggaaaaatt tgttttttcg tagataacgt ttcttaccta aaaagcggtt
     2761 tttaacctga tagttgagat aacctggttt atacaataaa ttttcaaatg agataatttt
     2821 taataacttt aaaaaaaaat ttttttatat cgttggtttt cgaaacttga ttttcagcaa
     2881 atttttaaaa tttgaatcaa cttgctctca aagacacgtt tattgtctta cgacttttaa
     2941 aaccggagta aatttaattt ttcgatgaat caatttcaga taaatctcag aacaagcact
     3001 ttactgtttc ttacttgttt tatgcaattt accatttcaa gttttctatg cccccgcgtt
     3061 cttttaccaa acgccttcca gtggacactc tagaatttct tcagcacaag aaggcgtggc
     3121 ctctgaaaag gcggagcaaa gtgcgcggag cccaagttag tggagaagac ggaccagtat
     3181 ataagccacg agcagtgctc agtccacacg gcgcgcctct agaggatccc cgggattggc
     3241 caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg
     3301 acccttggct aggatgggcc ctaaaaagaa gcgtaaagct agcatgaagc tgttgtcttc
     3361 aatggaacaa gcttgcgaca tatgccggtt aaaaaaactc aggtgctcga aggaaaagcc
     3421 caagtgtgcc aagtgtctga agaacaactc ggagtgccgt tattctccca aggctatgag
     3481 gtccccactg accagggcgc atctgacaga ggtagaatca aggttggaaa aattggaaga
     3541 tttgtttctg ctcatgtttc ctcgggaaaa cctggacagc attttgaaca tggactcttt
     3601 ggacgaagtg aaagtaatgt tgaaacaatt atatatgcag gactatggtg acacggatgc
     3661 tgctatggat agcttgaccc ctatggaaaa tggcatgtct gaagggccga gaaaacacat
     3721 gaagactgcg acatcatcgc caaaagtgga tgaagatgga agccaaagtc agttgagtgt
     3781 atcgccggaa ttcccgctgc agtctggtgg cggaggggct cctaggggtt ccggacgggc
     3841 tgacgcattg gacgattttg atctggatat gctgggaagt gacgccctcg atgattttga
     3901 ccttgacatg cttggttcgg atgcccttga tgactttgac ctcgacatgc tcggcagtga
     3961 cgcccttgat gatttcgacc tggacatgct gattaactaa ggtaccgaat tcgctagccg
     4021 gccatacaag taatccggat gatcgacgcc aacgtcgttg aattttcaaa ttttaaatac
     4081 tgaatatttg ttttttttcc tattatttat ttattctctt tgtgtttttt ttcttgcttt
     4141 ctaaaaaatt aattcaatcc aaatctaaac attttttttt ctctttccgt ctcccaattc
     4201 gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct cgctttcatt
     4261 tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat gatggaaatg
     4321 aaataaggac acacaatatg caacaacatt caattcagaa atatggagga aggtttaaaa
     4381 gaaaacataa aaatatatag aggaggaagg aaaactagta aaaaataagc aaagaaatta
     4441 ggcgaacgat gagaattgtc ctcgcttggg cccaaaaggc cttttttttt tggcgccgac
     4501 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4561 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4621 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4681 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4741 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4801 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4861 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4921 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     4981 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5041 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5101 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5161 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5281 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     5821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     6061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6301 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6361 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6421 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6481 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6541 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6601 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     6661 ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt
     6721 cagagcttaa aaatggctga aatcactcac aacgatggat acgctaacaa cttggaaatg
     6781 aaat
//
