LOCUS       Exported                4613 bp ds-DNA     circular SYN 13-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pHW394 cut with KpnI and EcoRI
            destabilized YFP from Pei
            Gibson ligation
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..405
                     /locus_tag="15xUAS"
                     /label=15xUAS
     misc_feature    302..318
                     /locus_tag="UAS"
                     /label=UAS
     misc_feature    522..702
                     /locus_tag="delta pes-10"
                     /label=delta pes-10
     misc_feature    757..798
                     /locus_tag="42bp intron"
                     /label=42bp intron
     misc_feature    838..1641
                     /locus_tag="YFP with introns"
                     /label=YFP with introns
     misc_feature    1705..1827
                     /locus_tag="Destabilized signal from mouse ornithine
                     decarboxyl"
                     /label=Destabilized signal from mouse ornithine
                     decarb...
                     /label=Destabilized signal from mouse ornithine
                     decarboxyl
     misc_feature    1866..2298
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             2661..3320
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      3418..4100
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    4440..4462
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     4468..4488
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    4502..4552
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(4553..4613,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt agaaaaaatg
      841 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      901 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      961 cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac
     1021 atatatatac taactaaccc tgattattta aattttcagc caacacttgt cactactttc
     1081 ggttatggtc ttcaatgctt cgccagatac ccagatcata tgaaacagca tgactttttc
     1141 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1201 aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat atttaaattt
     1261 tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     1321 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     1381 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac
     1441 atgattttac taactaacta atctgattta aattttcaga acttcaaaat tagacacaac
     1501 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     1561 ggccctgtcc ttttaccaga caaccattac ctgtcctacc aatctgccct ttcgaaagat
     1621 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagcggc cgcgattaca
     1681 catggcatgg atgaactata caaaagccat ggcttcccgc cggcggtggc ggcgcaggat
     1741 gatggcacgc tgcccatgtc ttgtgcccag gagagcggga tggaccgtca ccctgcagcc
     1801 tgtgcttctg ctaggatcaa tgtgtagcat tcgtagaatt cgctagccgg ccatacaagt
     1861 aatccggatg atcgacgcca acgtcgttga attttcaaat tttaaatact gaatatttgt
     1921 tttttttcct attatttatt tattctcttt gtgttttttt tcttgctttc taaaaaatta
     1981 attcaatcca aatctaaaca tttttttttc tctttccgtc tcccaattcg tattccgctc
     2041 ctctcatctg aacacaatgt gcaagtttat ttatcttctc gctttcattt cattaggacg
     2101 tggggggaat tggtggaagg gggaaacaca caaaaggatg atggaaatga aataaggaca
     2161 cacaatatgc aacaacattc aattcagaaa tatggaggaa ggtttaaaag aaaacataaa
     2221 aatatataga ggaggaagga aaactagtaa aaaataagca aagaaattag gcgaacgatg
     2281 agaattgtcc tcgcttgggc ccaaaaggcc tttttttttt ggcgccgacg tcaggtggca
     2341 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2401 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2461 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2521 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2581 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2641 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2701 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2761 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2821 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2881 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2941 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3001 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3061 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3121 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3181 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     3241 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3301 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3361 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3421 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3481 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3601 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     3661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3721 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     3901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4081 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4141 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4201 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     4261 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4321 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     4381 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     4441 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     4501 tgtaagttta aacatgatct tactaactaa ctattctcat ttaaattttc agagcttaaa
     4561 aatggctgaa atcactcaca acgatggata cgctaacaac ttggaaatga aat
//
