LOCUS       Exported                5336 bp ds-DNA     circular SYN 13-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pHW394 cut with KpnI and EcoRI
            iC1C2-TS-EYFP from pHW308
            Gibson ligation
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..405
                     /locus_tag="15xUAS"
                     /label=15xUAS
     misc_feature    302..318
                     /locus_tag="UAS"
                     /label=UAS
     misc_feature    522..702
                     /locus_tag="delta pes-10"
                     /label=delta pes-10
     misc_feature    757..798
                     /locus_tag="42bp intron"
                     /label=42bp intron
     misc_feature    838..1764
                     /locus_tag="bC1-C2"
                     /label=bC1-C2
     misc_feature    1774..1833
                     /locus_tag="TS"
                     /label=TS
     misc_feature    1834..2550
                     /locus_tag="eYFP"
                     /label=eYFP
     misc_feature    1834..2224
                     /locus_tag="EYFP"
                     /label=EYFP
     misc_feature    2226..2550
                     /locus_tag="EYFP(1)"
                     /label=EYFP
                     /label=EYFP(1)
     misc_feature    2589..3021
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             3384..4043
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      4141..4823
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    5163..5185
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     5191..5211
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    5225..5275
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(5276..5336,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt agaaaaaatg
      841 gactatggcg gcgctttgtc tgccgtcgga ctcttccaaa cctcatacac tcttgagaac
      901 aatggttctg ttatttgcat cccgaacaac ggccagtgct tctgcttggc ttggcttaaa
      961 tccaacggaa caaatgccga gaagttggct gccaacattc tgcagtggat ttcttttgcg
     1021 ctttcagcgc tctgcctgat gttctacggc taccagacct ggaagtctac ttgcggctgg
     1081 gaggagattt acgtggccac gatctcgatg atcaagttca tcatcgagta tttccatagc
     1141 tttgacgaac ctgcggtgat ctactcatcc aacggcaaca agaccaagtg gcttcgttac
     1201 gcgagctggc tgctgacctg ccctgtcatt cttatccgtc tgagcaacct tacgggtctg
     1261 gcgaacgact ataacaagcg taccatgggt ctgctggtgt cagatatcgg cacgatcgtg
     1321 tggggcacca cggccgcgct gtccaaggga tacgtccgtg tcattttctt cctgatgggc
     1381 ctgtgctacg gcatctacac attcttcaac gcagccaagg tctacattga ggcgtaccac
     1441 accgtgccga agggccggtg tcgccaggtg gtgactggca tggcttggct cttcttcgta
     1501 tcatggggta tgttccccat cctgttcatc ctcggccccg agggcttcgg cgtcctgagc
     1561 aagtacggct ccaacgtcgg ccacaccatc attgacctga tgtcgaagca gtgctggggt
     1621 ctgctcggcc actacctgcg cgtgctgatc cacgagcata tcctcatcca cggcgacatt
     1681 cgcaagacca ccaaattgaa cattggtggc actgagattg aggtcgagac gctggtggag
     1741 gacgaggccg aggctggcgc ggtcgcggcc gccaagagca ggatcaccag cgagggcgag
     1801 tacatccccc tggaccagat cgacatcaac gtggtgagca agggcgagga gctgttcacc
     1861 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1921 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1981 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgcag
     2041 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2101 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2161 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2221 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2281 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2341 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2401 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     2461 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2521 actctcggca tggacgagct gtacaagtaa cattcgtaga attcgctagc cggccataca
     2581 agtaatccgg atgatcgacg ccaacgtcgt tgaattttca aattttaaat actgaatatt
     2641 tgtttttttt cctattattt atttattctc tttgtgtttt ttttcttgct ttctaaaaaa
     2701 ttaattcaat ccaaatctaa acattttttt ttctctttcc gtctcccaat tcgtattccg
     2761 ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt ctcgctttca tttcattagg
     2821 acgtgggggg aattggtgga agggggaaac acacaaaagg atgatggaaa tgaaataagg
     2881 acacacaata tgcaacaaca ttcaattcag aaatatggag gaaggtttaa aagaaaacat
     2941 aaaaatatat agaggaggaa ggaaaactag taaaaaataa gcaaagaaat taggcgaacg
     3001 atgagaattg tcctcgcttg ggcccaaaag gccttttttt tttggcgccg acgtcaggtg
     3061 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3181 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3241 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3301 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3361 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3421 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3481 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3541 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3601 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3661 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3721 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3781 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3841 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3901 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3961 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     4021 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     4081 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4141 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4201 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4261 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4321 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4381 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4441 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4501 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4561 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
     4621 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4681 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4741 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4801 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4861 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4921 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4981 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     5041 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5101 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5161 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     5221 agctgtaagt ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt
     5281 aaaaatggct gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaat
//
