LOCUS       Exported                5243 bp ds-DNA     circular SYN 13-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pDO001 15xUAS-miniSOG103L-VAMP2-mCherry-let-858 3'UTR
            
            Vector (3.6k bp): pHW394 pPD117.01-15xUAS-GFP-let-858 3'UTR
            Insert (1.6k bp):  Pglr-3s-miniSOG103L-VAMP2-mCherry from He Liu and
            Yun Zhang at Harvard Univeristy
            RE: repalce GFP  with (AgeI or KpnI ) and EcoRI to create new cGAL 
            effetor
            Oligo used: oDO003f and oDO002r
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..405
                     /locus_tag="15xUAS"
                     /label=15xUAS
     misc_feature    302..318
                     /locus_tag="UAS"
                     /label=UAS
     misc_feature    522..702
                     /locus_tag="delta pes-10"
                     /label=delta pes-10
     misc_feature    757..798
                     /locus_tag="42bp intron"
                     /label=42bp intron
     misc_feature    838..1155
                     /locus_tag="likely to be miniSOG103L (Han)"
                     /label=likely to be miniSOG103L (Han)
     misc_feature    1195..1593
                     /locus_tag="VAMP2"
                     /label=VAMP2
     misc_feature    join(1594..1749,1801..1994,2046..2238,2290..2454)
                     /locus_tag="mCherry(worm) with introns"
                     /label=mCherry(worm) with introns
     misc_feature    2496..2928
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             3291..3950
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      4048..4730
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    5070..5092
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     5098..5118
                     /locus_tag="M13-rev"
                     /label=M13-rev
     misc_feature    5132..5182
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(5183..5243,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt agaaaaaatg
      841 gagaaaagtt tcgtgataac tgatccacgg ctgccagaca atcccatcat cttcgcatcc
      901 gatggcttcc tggagctgac cgagtattcc agagaggaga tcctgggccg caatggccgc
      961 tttctgcagg gaccagagac agaccaggcc acagtgcaga agattcgcga tgccattaga
     1021 gatcagcgcg agattaccgt gcagctgata aactacacaa aaagcgggaa gaaattctgg
     1081 aacctcctgc acctccagcc catgagggac cagaagggtg agctccagta tttcatcgga
     1141 gtgctgctgg atggatcagg aggaggctca ggaggaggag gcgcgcctac cggtatgtcg
     1201 gctaccgctg ccaccgtccc gcctgccgcc ccggccggcg agggtggccc ccctgcacct
     1261 cctccaaacc ttactagtaa caggagactg cagcagaccc aggcccaggt ggatgaggtg
     1321 gtggacatca tgagggtgaa tgtggacaag gtcctggagc gggaccagaa gttgtcggag
     1381 ctggatgacc gtgcagatgc cctccaggca ggggcctccc agtttgaaac aagtgcagcc
     1441 aagctcaagc gcaaatactg gtggaaaaac ctcaagatga tgatcatctt gggagtgatc
     1501 tgcgccatca tcctcatcat catcatcgtt tacttcagca ctagcggcgg aagcggcggg
     1561 ggaatcgatg gaccggtaga aaaaatggga tccatggtct caaagggtga agaagataac
     1621 atggcaatta ttaaagagtt tatgcgtttc aaggtgcata tggagggatc tgtcaatggg
     1681 catgagtttg aaattgaagg tgaaggagaa ggccgaccat atgagggaac acaaaccgca
     1741 aaactaaagg taagtttaaa catatatata ctaactaacc ctgattattt aaattttcag
     1801 gtaactaaag gcggaccatt accattcgcc tgggacatcc tctctccaca gttcatgtat
     1861 ggaagtaaag cttatgttaa acatccggca gatataccag attatttgaa actttcattc
     1921 ccggagggtt ttaagtggga acgcgtaatg aattttgaag acggaggagt tgttacagtg
     1981 acgcaagact caaggtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
     2041 ttcagcctcc aagatggaga atttatttat aaagtcaaac ttcgaggaac gaatttcccc
     2101 tcggatggac ctgttatgca gaagaagact atgggatggg aagcttcaag tgaaagaatg
     2161 taccctgaag acggtgctct taagggagag attaaacaac gtcttaaatt gaaagatgga
     2221 ggacattacg atgctgaggt aagtttaaac atgattttac taactaacta atctgattta
     2281 aattttcagg tgaagacaac ttacaaagcc aaaaaaccag ttcagctgcc aggagcgtac
     2341 aatgttaata ttaaactgga tatcacctcc cacaacgagg attacactat cgttgagcaa
     2401 tatgaaagag ctgaagggcg gcactcgaca ggtggcatgg atgaattgta taagtagcat
     2461 tcgtagaatt cgctagccgg ccatacaagt aatccggatg atcgacgcca acgtcgttga
     2521 attttcaaat tttaaatact gaatatttgt tttttttcct attatttatt tattctcttt
     2581 gtgttttttt tcttgctttc taaaaaatta attcaatcca aatctaaaca tttttttttc
     2641 tctttccgtc tcccaattcg tattccgctc ctctcatctg aacacaatgt gcaagtttat
     2701 ttatcttctc gctttcattt cattaggacg tggggggaat tggtggaagg gggaaacaca
     2761 caaaaggatg atggaaatga aataaggaca cacaatatgc aacaacattc aattcagaaa
     2821 tatggaggaa ggtttaaaag aaaacataaa aatatataga ggaggaagga aaactagtaa
     2881 aaaataagca aagaaattag gcgaacgatg agaattgtcc tcgcttgggc ccaaaaggcc
     2941 tttttttttt ggcgccgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3001 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3061 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3121 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3181 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3241 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3301 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3361 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3421 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3481 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3541 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3601 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3661 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3721 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3781 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3841 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3901 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3961 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4021 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4261 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4501 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4741 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4801 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4861 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4921 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4981 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     5041 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     5101 gaaacagcta tgaccatgat tacgccaagc tgtaagttta aacatgatct tactaactaa
     5161 ctattctcat ttaaattttc agagcttaaa aatggctgaa atcactcaca acgatggata
     5221 cgctaacaac ttggaaatga aat
//
