LOCUS       Exported                5458 bp ds-DNA     circular SYN 13-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Note from Jasmin: Vector pJL046 (KpnI),
            PTX from pHW560
            Gibson ligation
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    36..52
                     /locus_tag="UAS(2)"
                     /label=UAS
                     /label=UAS(2)
     misc_feature    55..71
                     /locus_tag="UAS(8)"
                     /label=UAS
                     /label=UAS(8)
     misc_feature    74..90
                     /locus_tag="UAS(6)"
                     /label=UAS
                     /label=UAS(6)
     misc_feature    93..109
                     /locus_tag="UAS(12)"
                     /label=UAS
                     /label=UAS(12)
     misc_feature    112..117
                     /locus_tag="UAS"
                     /label=UAS
     misc_feature    119..128
                     /locus_tag="UAS(4)"
                     /label=UAS
                     /label=UAS(4)
     misc_feature    169..185
                     /locus_tag="UAS(15)"
                     /label=UAS
                     /label=UAS(15)
     misc_feature    188..204
                     /locus_tag="UAS(5)"
                     /label=UAS
                     /label=UAS(5)
     misc_feature    207..223
                     /locus_tag="UAS(7)"
                     /label=UAS
                     /label=UAS(7)
     misc_feature    226..242
                     /locus_tag="UAS(14)"
                     /label=UAS
                     /label=UAS(14)
     misc_feature    245..250
                     /locus_tag="UAS(10)"
                     /label=UAS
                     /label=UAS(10)
     misc_feature    252..261
                     /locus_tag="UAS(1)"
                     /label=UAS
                     /label=UAS(1)
     misc_feature    302..318
                     /locus_tag="UAS(17)"
                     /label=UAS
                     /label=UAS(17)
     misc_feature    321..337
                     /locus_tag="UAS(9)"
                     /label=UAS
                     /label=UAS(9)
     misc_feature    340..356
                     /locus_tag="UAS(13)"
                     /label=UAS
                     /label=UAS(13)
     misc_feature    359..375
                     /locus_tag="UAS(11)"
                     /label=UAS
                     /label=UAS(11)
     misc_feature    378..383
                     /locus_tag="UAS(16)"
                     /label=UAS
                     /label=UAS(16)
     misc_feature    385..394
                     /locus_tag="UAS(3)"
                     /label=UAS
                     /label=UAS(3)
     misc_feature    835..1545
                     /locus_tag="PTX sequence from pKMC 269"
                     /label=PTX sequence from pKMC 269
     misc_feature    1555..1804
                     /locus_tag="SL2"
                     /label=SL2
     misc_feature    1814..2680
                     /locus_tag="GFP"
                     /label=GFP
     misc_feature    1983..2032
                     /locus_tag="intron1"
                     /label=intron1
     misc_feature    2187..2237
                     /locus_tag="intron2"
                     /label=intron2
     misc_feature    2402..2452
                     /locus_tag="intron3"
                     /label=intron3
     misc_feature    2711..3143
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             3506..4165
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      4263..4945
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    5285..5307
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     5313..5333
                     /locus_tag="M13-rev"
                     /label=M13-rev
     intron          5348..5397
                     /locus_tag="decoy intron"
                     /label=decoy intron
     promoter        join(5398..5458,1..2)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt aaaaatggac
      841 gatcctcccg ccaccgtata ccgctatgac tcccgcccgc cggaggacgt tttccagaac
      901 ggattcacgg cgtggggaaa caacgacaat gtgctcgacc atctgaccgg acgttcctgc
      961 caggtcggca gcagcaacag cgctttcgtc tccaccagca gcagccggcg ctataccgag
     1021 gtctatctcg aacatcgcat gcaggaagcg gtcgaggccg aacgcgccgg caggggcacc
     1081 ggccacttca tcggctacat ctacgaagtc cgcgccgaca acaatttcta cggcgccgcc
     1141 agctcgtact tcgaatacgt cgacacttat ggcgacaatg ccggccgtat cctcgccggc
     1201 gcgctggcca cctaccagag cgaatatctg gcacaccggc gcattccgcc cgaaaacatc
     1261 cgcagggtaa cgcgggtcta tcacaacggc atcaccggcg agaccacgac cacggagtat
     1321 tccaacgctc gctacgtcag ccagcagact cgcgccaatc ccaaccccta cacatcgcga
     1381 aggtccgtag cgtcgatcgt cggcacattg gtgcgcatgg cgccggtgat aggcgcttgc
     1441 atggcgcggc aggccgaaag ctccgaggcc atggcagcct ggtccgaacg cgccggcgag
     1501 gcgatggttc tcgtgtacta cgaaagcatc gcgtattcgt tctagggtac cgtagctgtc
     1561 tcatcctact ttcacctagt taactgcttg tcttaaaatc tatgcttctc tttagtatct
     1621 aaaattttcc tagaagctta caagtatata aatggtctct tctcaataaa ggttgtatat
     1681 ttattcatct tattgaatct gccatttcct cgtttttgcg agtttatata ccttccaatt
     1741 ttctttctat tgtattttca acttctaatt ttaattcagg gaaactgctg taccggtaga
     1801 aaaaatgctc gagagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     1861 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     1921 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     1981 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc agccaacact
     2041 tgtcactact ttctgttatg gtgttcaatg cttctcgaga tacccagatc atatgaaacg
     2101 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     2161 caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacta actaaccata
     2221 catatttaaa ttttcaggtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     2281 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     2341 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     2401 tgtaagttta aacttggact tactaactaa cggattatat ttaaattttc agaacttcaa
     2461 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     2521 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     2581 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     2641 tgctgggatt acacatggca tggatgaact atacaaatag gaattcgcta gccggccata
     2701 caagtaatcc ggatgatcga cgccaacgtc gttgaatttt caaattttaa atactgaata
     2761 tttgtttttt ttcctattat ttatttattc tctttgtgtt ttttttcttg ctttctaaaa
     2821 aattaattca atccaaatct aaacattttt ttttctcttt ccgtctccca attcgtattc
     2881 cgctcctctc atctgaacac aatgtgcaag tttatttatc ttctcgcttt catttcatta
     2941 ggacgtgggg ggaattggtg gaagggggaa acacacaaaa ggatgatgga aatgaaataa
     3001 ggacacacaa tatgcaacaa cattcaattc agaaatatgg aggaaggttt aaaagaaaac
     3061 ataaaaatat atagaggagg aaggaaaact agtaaaaaat aagcaaagaa attaggcgaa
     3121 cgatgagaat tgtcctcgct tgggcccaaa aggccttttt tttttggcgc cgacgtcagg
     3181 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3241 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3301 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     3361 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3421 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     3481 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     3541 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3601 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3661 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3721 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3781 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3841 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     3901 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     3961 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4021 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4081 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4141 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4201 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4261 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4321 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4381 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4441 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     4501 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4561 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4621 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4681 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     4741 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4801 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4861 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4921 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4981 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     5041 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     5101 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     5161 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     5221 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     5281 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     5341 caagctgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagagc
     5401 ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaat
//
