LOCUS       Exported                6115 bp ds-DNA     linear   UNA 15-FEB-2023
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Note from Jasmin: Vector pJL049 (KpnI/EcoRI),
            TeTX  from pHW491
            Gibson ligation
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        join(1..2,6055..6115)
                     /locus_tag="decoy ORF"
                     /label=decoy ORF
     misc_feature    36..52
                     /locus_tag="UAS(2)"
                     /label=UAS
                     /label=UAS(2)
     misc_feature    55..71
                     /locus_tag="UAS(14)"
                     /label=UAS
                     /label=UAS(14)
     misc_feature    74..90
                     /locus_tag="UAS(12)"
                     /label=UAS
                     /label=UAS(12)
     misc_feature    93..109
                     /locus_tag="UAS(10)"
                     /label=UAS
                     /label=UAS(10)
     misc_feature    112..117
                     /locus_tag="UAS"
                     /label=UAS
     misc_feature    119..128
                     /locus_tag="UAS(4)"
                     /label=UAS
                     /label=UAS(4)
     misc_feature    169..185
                     /locus_tag="UAS(11)"
                     /label=UAS
                     /label=UAS(11)
     misc_feature    188..204
                     /locus_tag="UAS(5)"
                     /label=UAS
                     /label=UAS(5)
     misc_feature    207..223
                     /locus_tag="UAS(6)"
                     /label=UAS
                     /label=UAS(6)
     misc_feature    226..242
                     /locus_tag="UAS(8)"
                     /label=UAS
                     /label=UAS(8)
     misc_feature    245..250
                     /locus_tag="UAS(7)"
                     /label=UAS
                     /label=UAS(7)
     misc_feature    252..261
                     /locus_tag="UAS(1)"
                     /label=UAS
                     /label=UAS(1)
     misc_feature    302..318
                     /locus_tag="UAS(13)"
                     /label=UAS
                     /label=UAS(13)
     misc_feature    321..337
                     /locus_tag="UAS(9)"
                     /label=UAS
                     /label=UAS(9)
     misc_feature    340..356
                     /locus_tag="UAS(16)"
                     /label=UAS
                     /label=UAS(16)
     misc_feature    359..375
                     /locus_tag="UAS(15)"
                     /label=UAS
                     /label=UAS(15)
     misc_feature    378..383
                     /locus_tag="UAS(17)"
                     /label=UAS
                     /label=UAS(17)
     misc_feature    385..394
                     /locus_tag="UAS(3)"
                     /label=UAS
                     /label=UAS(3)
     misc_feature    847..2220
                     /locus_tag="TeTx cDNA"
                     /label=TeTx cDNA
     misc_feature    1008
                     /locus_tag="C to T mutation in pHW491 L8.1 and 8.2 clones"
                     /label=C to T mutation in pHW491 L8.1 and 8.2
                     clones
                     /label=C to T mutation in pHW491 L8.1 and 8.2 clones
     misc_feature    2239..2488
                     /locus_tag="SL2"
                     /label=SL2
     CDS             join(2489..2692,2744..2902,2954..3118,3170..3337)
                     /locus_tag="mKate2"
                     /label=mKate2
     misc_feature    3368..3800
                     /locus_tag="let-858 3UTR"
                     /label=let-858 3UTR
                     /label=nonstandard type:  UTR
     CDS             4163..4822
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      4920..5602
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_binding    5942..5964
                     /locus_tag="LacO"
                     /label=LacO
     primer_bind     5970..5990
                     /locus_tag="M13-rev"
                     /label=M13-rev
     intron          6005..6054
                     /locus_tag="decoy intron"
                     /label=decoy intron
ORIGIN
        1 aagcttgcta gcccatgatt acgccaagct acgggcggag tactgtcctc cgagcggagt
       61 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagactcc ctagcccatg attacgccaa gctacgggcg gagtactgtc
      181 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
      241 cgagcggagt actgtcctcc gagcggagac tccctagccc atgattacgc caagctacgg
      301 gcggagtact gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg
      361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gactccctag gggcggccgc
      421 actgactggg ccggccaaaa atgcataagg ttttgctggc actgttcttt atctttctgg
      481 caccagcatc cgcactggca gtctccgaac cggcctgcag gtcgattttt tgcaaattac
      541 gagcgttgta gggggcggag cgataggtcc tataggtttt ggtatatcat cattcattca
      601 ttcattggta cattcattta cccaccttcc tctttctgag cttctctgga gttctgtgct
      661 tcctttttcc cttatcttta tactgtaatt tttaactttc aggcattgat tggcgcgcct
      721 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
      781 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtaccggt gcgatcgcta
      841 gcaaaaatgc cgatcaccat caacaacttc cgttactccg acccggttaa caacgacacc
      901 atcatcatga tggaaccgcc gtactgcaaa ggtctagaca tctactacaa agccttcaaa
      961 atcaccgacc gtatctggat cgttccggaa cgttacgaat ttggtaccaa accggaagac
     1021 ttcaacccgc cgtcctccct gatcgaaggt gcttccgaat actacgaccc gaactacctg
     1081 cgtaccgact ccgacaaaga ccgtttcctg cagaccatgg ttaaactgtt caaccgtatc
     1141 aaaaacaacg ttgctggtga agctctgctg gacaaaatca tcaacgctat cccgtacctg
     1201 ggtaactcct actccctgct ggacaaattc gacaccaact ccaactccgt ttccttcaac
     1261 ctgctggaac aggatccgtc cggtgctacc accaaatccg ctatgctgac caacctgatc
     1321 atcttcggtc cgggtccggt tctgaacaaa aacgaagttc gtggtatcgt tctgcgtgtt
     1381 gacaacaaaa actacttccc gtgccgtgac ggtttcggtt ccatcatgca gatggctttc
     1441 tgcccggaat acgtaccgac cttcgacaac gttatcgaaa acatcacctc cctgaccatc
     1501 ggtaaatcca aatacttcca ggacccggct ctgctgctga tgcacgaact gatccacgtt
     1561 ctgcacggtc tgtacggcat gcaggtttcc tcccacgaaa tcatcccgtc caaacaggaa
     1621 atctacatgc agcacaccta cccgatctcc gctgaagagc tcttcacctt cggtggtcag
     1681 gacgctaacc tgatctccat cgatatcaaa aacgacctgt acgaaaaaac cctgaacgac
     1741 tacaaagcta tcgctaacaa actgtcccag gttacctcct gcaacgaccc gaacatcgac
     1801 atcgactcct acaaacagat ctatcagcag aagtatcagt ttgataagga ttccaacggc
     1861 cagtatatag tgaacgagga taagtttcag atactgtata actccataat gtatggcttt
     1921 actgagatag agctgggcaa gaagtttaac ataaagactc gcttaagcta tttttccatg
     1981 aaccacgatc ctgtgaagat acctaacctg ctggatgata ctatatataa cgatactgag
     2041 ggctttaaca tagagtccaa ggatctgaag tccgagtata agggccagaa catgagggtg
     2101 aacactaacg cctttaggaa cgtcgacggt tccggtctgg tttccaaact gatcggtctg
     2161 tgcaaaaaaa tcatcccgcc gaccaacatc cgtgaaaacc tgtacaaccg taccgcttaa
     2221 cctgcaggtt ttgaattcgc tgtctcatcc tactttcacc tagttaactg cttgtcttaa
     2281 aatctatgct tctctttagt atctaaaatt ttcctagaag cttacaagta tataaatggt
     2341 ctcttctcaa taaaggttgt atatttattc atcttattga atctgccatt tcctcgtttt
     2401 tgcgagttta tataccttcc aattttcttt ctattgtatt ttcaacttct aattttaatt
     2461 cagggaaact gctgtaccgg tagaaaaaat ggtctccgag ctcattaaag aaaacatgca
     2521 tatgaagctc tacatggagg gaaccgtcaa caaccaccac ttcaagtgca cctccgaggg
     2581 agagggaaag ccatacgagg gaacccaaac catgcgtatc aaggccgtcg agggaggacc
     2641 actcccattc gccttcgaca tcctcgccac ctccttcatg tacggatcca aggtaagttt
     2701 aaacatatat atactaacta accctgatta tttaaatttt cagaccttca tcaaccacac
     2761 ccaaggaatc ccagacttct tcaagcaatc cttcccagag ggattcacct gggagcgtgt
     2821 caccacctac gaggacggag gagtcctcac cgccacccaa gacacctccc tccaagacgg
     2881 atgcctcatc tacaacgtca aggtaagttt aaacagttcg gtactaacta accatacata
     2941 tttaaatttt cagatccgtg gagtcaactt cccatccaac ggaccagtca tgcaaaagaa
     3001 gaccctcgga tgggaggcct ccaccgagac cctctaccca gccgacggag gacttgaggg
     3061 acgtgccgac atggccctca agctcgtcgg aggaggacac ctcatctgca acctcaaggt
     3121 aagtttaaac atgattttac taactaacta atctgattta aattttcaga ccacctaccg
     3181 ttccaagaag ccagccaaga acctcaagat gccaggagtc tactacgtcg accgtcgtct
     3241 tgagcgtatc aaggaggccg acaaggagac ctacgtcgag caacacgagg tcgccgtcgc
     3301 ccgttactgc gacctcccat ccaagctcgg acaccgttaa ttcgctagcc ggccatacaa
     3361 gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa attttaaata ctgaatattt
     3421 gttttttttc ctattattta tttattctct ttgtgttttt tttcttgctt tctaaaaaat
     3481 taattcaatc caaatctaaa catttttttt tctctttccg tctcccaatt cgtattccgc
     3541 tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat ttcattagga
     3601 cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat gaaataagga
     3661 cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa agaaaacata
     3721 aaaatatata gaggaggaag gaaaactagt aaaaaataag caaagaaatt aggcgaacga
     3781 tgagaattgt cctcgcttgg gcccaaaagg cctttttttt ttggcgccga cgtcaggtgg
     3841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4141 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4201 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5101 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5341 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc
     5401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5521 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5581 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5641 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5701 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5761 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     5821 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     5881 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     5941 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     6001 gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt tcagagctta
     6061 aaaatggctg aaatcactca caacgatgga tacgctaaca acttggaaat gaaat
//
