LOCUS       0986_TOPO_Blunt        5885 bp DNA     circular SYN 22-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pTopo_Ex2Donor_pPGK-PURO
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Hannah Crimmins
  TITLE     Direct Submission
  JOURNAL   Exported Feb 22, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     66..90
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     GGCGCGGAGGTtTCCAGGAAGGCGGGCA"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 143 .. 148 / #ffffff / -35
                        2: 149 .. 166 / #ffffff
                        3: 167 .. 173 / #ffffff / -10"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             217..3254
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /note="color: #993366"
                     /translation="MTMITPSYLGDTIEYSSYASSLVPSSDPLVTAASVLEFALVAQCL
                     VCVPWDARPPPAAPSFRQVGLPGVGVRLGLRAAGGNQRHAESSAGDSGRFPRRCPA*RS
                     WWPECCRGCASAARRTCWPSASRCWTGPAGAPPRPSPPA*AATCPTR*PTHCGGAGRGG
                     CCCAAWATTCWFTCWHAARSLCWWLPAAPTRCAGRRCTSSALPLRPGPRHTQKGPK*MN
                     EPGRGGAFPKAVWSMRFSSPAGHLALHKWPLASHTFHIHR*APTGSVLWWPLRATFYSS
                     PSQEVPPRPAARVVQDVTNGSSTSH*SRADGQHR*AMEAGRPLGQRPIAALLLRFLGSE
                     AGKGWVRGRAQGRAQGRGGRPKVLRRPGILHASKAHVCRAVLLFLISGPFDLHPSRSRA
                     AEAYHDRVQAHGAPRHPRRRPQGRTHPRRRVRRLPRHAPHRRSGPPHRAGHRAARTLPH
                     ARRARHRQGVGRGRRRRGGGLDHAGERRSGGGVRRDRPAHGRVERFPAGRAATDGRPPG
                     AAPAQGARVVPGHRRRLARPPGQGSGQRRRAPRSGGGRARRGARLPGNLRAPQPPLLRA
                     ARLHRHRRRRGARRTAHLVHDPQARCLSGRNLFIAAYNGYK*SNSITNFTNKAFFSLHS
                     SCGLSKLINVS*GPFLVDPEGVWDANGPGTIASGRPGSPWACQPRVRGGAGAVPAEVCR
                     CPRGPGVALPLSRSGRPLGRGPGPTRAGRVDRVTVVSVWCHLPDPPKKPPLWRVRSLAR
                     ATPTHPWAASTTRAPHPHRGHHVPGTRLVPRCTPRPSTSSTPQATRSSCGPPSYSAL*G
                     PA*LALGGSWRPSFWVPRANSADIHHTGGRSSMHLEGPIRPIVSRITIHWPSFYNVVTG
                     KTLALPNLIALQHIPLSPAGVIAKRPAPIALPNSCAAYTYGSLRFTPIKERAVIVCLWM
                     YRVILLTRRGDGW*SPWPVHVCCQIKSPVNFTRWCISGMKAGA**PPIWPVCRSPLSGK
                     KWLISATAKMTSKTPLT*CSGEY"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    334..336
                     /label=Cut here
                     /note="color: #a6acb3"
     misc_feature    337..859
                     /label=Left Arm Donor
                     /note="color: #cc99ff"
     misc_feature    337..405
                     /label=ex 1
                     /note="color: #800000"
     primer_bind     337..355
                     /label=Left Arm Fwd 3
                     /note="color: black; sequence: GTGGCCCAGTGCCTGGTGT"
     primer_bind     343..361
                     /label=Left Arm Fwd2
                     /note="color: black; sequence: CAGTGCCTGGTGTGCGTGC"
     misc_feature    510..2702
                     /label=ex 2
                     /note="color: #800000"
     primer_bind     630..660
                     /label=AleI mutx primerFwd
                     /note="color: black; sequence: 
                     GCCTTCACCACCAGCGTAAGCAGCTACCTGC"
     primer_bind     complement(630..660)
                     /label=AleI mutx primerRev
                     /note="color: black; sequence: 
                     GCAGGTAGCTGCTTACGCTGGTGGTGAAGGC"
     misc_feature    647..648
                     /label=gc>aa (in donor)
                     /note="color: #ff0000"
     misc_feature    649..669
                     /label=gRNA5
                     /note="This forward directional feature has 2 segments:
                        1: 649 .. 666 / #339966
                        2: 667 .. 669 / #ffff00"
     primer_bind     complement(776..793)
                     /label=MUTRX Rev
                     /note="color: black; sequence: TAGGCGCAGCTGGGAGCC"
     misc_feature    843..2173
                     /label=gRNA3
                     /note="This forward directional feature has 2 segments:
                        1:  843 .. 2170 / #339966
                        2: 2171 .. 2173 / #ffff00"
     misc_feature    complement(852..2182)
                     /label=gRNA 1
                     /note="This reverse directional feature has 2 segments:
                        1:  852 ..  854 / #ffff00
                        2:  855 .. 2182 / #339966"
     misc_feature    860..2167
                     /label=PURO DROP IN
                     /note="color: #ff6600"
     primer_bind     860..902
                     /label=puro fwd hc
                     /note="color: black; sequence: 
                     aaaagggtcccaaatgaatgaatgaaccGGGTAGGGGAGGCgc"
     primer_bind     885..900
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: ATTCTACCGGGTAGGGGAGGC"
     promoter        888..1387
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    948
                     /label=extra g (addgene seq)
                     /note="color: #a6acb3"
     misc_feature    1235..1236
                     
                     /note="color: #a6acb3"
     misc_feature    1248
                     /label=extra g - from addgene
                     /note="color: #a6acb3"
     CDS             1427..2026
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     1890..1907
                     /label=geno PURO fwd
                     /note="color: black; sequence: gggTGCCCGCCTTCCTGG"
     primer_bind     complement(1893..1920)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     GGCGCGGAGGTtTCCAGGAAGGCGGGCA"
     primer_bind     1903..1926
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: CCTGGAaACCTCCGCGCCCCGCAA"
     primer_bind     complement(2009..2095)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     ttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaag
                     ttgcggccgcTCAGGCACCGGGCTTGCG"
     polyA_signal    2035..2156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(2135..2167)
                     /label=puro rev HC
                     /note="color: black; sequence: 
                     aaaaGGGACCCtaagatacattgatgagtttgg"
     misc_feature    2168..2201
                     
                     /note="color: #cc99ff"
     misc_feature    complement(2175..2197)
                     /label=gRNA4
                     /note="This reverse directional feature has 2 segments:
                        1: 2175 .. 2177 / #ffff00
                        2: 2178 .. 2197 / #339966"
     misc_feature    2179..2201
                     /label=gRNA2
                     /note="This forward directional feature has 2 segments:
                        1: 2179 .. 2198 / #339966
                        2: 2199 .. 2201 / #ffff00"
     primer_bind     complement(2191..2215)
                     /label=Left Arm Rev2
                     /note="color: black; sequence: CTATGGTTCCAGGCCCGTTCGCATC"
     misc_feature    2202..2702
                     /label=Right Arm Donor
                     /note="color: #cc99ff"
     primer_bind     2202..2221
                     /label=Right Arm Fwd1
                     /note="color: black; sequence: GCCTGGAACCATAGCGTCAG"
     primer_bind     2204..2223
                     /label=MUTRX Fwd
                     /note="color: black; sequence: CTGGAACCATAGCGTCAGGG"
     primer_bind     complement(2204..2223)
                     /label=Left Arm Rev3
                     /note="color: black; sequence: CCCTGACGCTATGGTTCCAG"
     primer_bind     complement(2278..2299)
                     /label=geno Rev RAD
                     /note="color: black; sequence: AACGGCAGACTTCGGCTGGCAC"
     primer_bind     complement(2627..2651)
                     /label=Right Arm Rev1
                     /note="color: black; sequence: CCTCAGAGAGCTGAGTAGGAAGGAG"
     primer_bind     complement(2679..2701)
                     /label=Right Arm Rev
                     /note="color: black; sequence: GAACCCAGAAAGATGGTCTCCAC"
     misc_feature    2703..2705
                     /label=Cut here
                     /note="color: #a6acb3"
     promoter        complement(2773..2791)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(2798..2814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(2798..2814)
                     /label=Primer 3
                     /note="color: black; sequence: GTAAAACGACGGCCAGT"
     CDS             3603..4397
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      4598..4607
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             4604..4978
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      5116..5704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttgtgg cccagtgcct ggtgtgcgtg
      361 ccctgggacg cacggccgcc ccccgccgcc ccctccttcc gccaggtggg cctccccggg
      421 gtcggcgtcc ggctggggtt gagggcggcc ggggggaacc agcgacatgc ggagagcagc
      481 gcaggcgact cagggcgctt cccccgcagg tgtcctgcct gaaggagctg gtggcccgag
      541 tgctgcagag gctgtgcgag cgcggcgcga agaacgtgct ggccttcggc ttcgcgctgc
      601 tggacggggc ccgcgggggc ccccccgagg ccttcaccac cagcgtaagc agctacctgc
      661 ccaacacggt gaccgacgca ctgcggggga gcggggcgtg ggggctgctg ctgcgccgcg
      721 tgggcgacga cgtgctggtt cacctgctgg cacgctgcgc gctctttgtg ctggtggctc
      781 ccagctgcgc ctaccaggtg tgcgggccgc cgctgtacca gctcggcgct gccactcagg
      841 cccggccccc gccacacgca aaagggtccc aaatgaatga atgaaccggg taggggaggc
      901 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
      961 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     1021 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     1081 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     1141 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     1201 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     1261 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     1321 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     1381 cctttcgacc tgcatccatc tagatctcga gcagctgaag cttaccatga ccgagtacaa
     1441 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     1501 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     1561 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     1621 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     1681 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     1741 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     1801 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     1861 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaaa cctccgcgcc
     1921 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     1981 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgagcgg ccgcaacttg
     2041 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2101 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttagggt
     2161 ccctttttag tggaccccga aggcgtctgg gatgcgaacg ggcctggaac catagcgtca
     2221 gggaggccgg ggtccccctg ggcctgccag ccccgggtgc gaggaggcgc gggggcagtg
     2281 ccagccgaag tctgccgttg cccaagaggc ccaggcgtgg cgctgcccct gagccggagc
     2341 ggacgcccgt tgggcagggg tcctgggccc acccgggcag gacgcgtgga ccgagtgacc
     2401 gtggtttctg tgtggtgtca cctgccagac ccgccgaaga agccacctct ttggagggtg
     2461 cgctctctgg cacgcgccac tcccacccat ccgtgggccg ccagcaccac gcgggccccc
     2521 catccacatc gcggccacca cgtccctggg acacgccttg tcccccggtg tacgccgaga
     2581 ccaagcactt cctctactcc tcaggcgaca aggagcagct gcggccctcc ttcctactca
     2641 gctctctgag gcccagcctg actggcgctc ggaggctcgt ggagaccatc tttctgggtt
     2701 ccaagggcga attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag
     2761 ggcccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     2821 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     2881 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     2941 tatacgtacg gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     3001 tggatgtaca gagtgatatt attgacacgc cggggcgacg gatggtgatc cccctggcca
     3061 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     3121 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     3181 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     3241 tctggggaat ataaatgtca ggcatgagat tatcaaaaag gatcttcacc tagatccttt
     3301 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg
     3361 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta
     3421 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg
     3481 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc
     3541 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc
     3601 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     3661 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     3721 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     3781 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     3841 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     3901 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     3961 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4021 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4081 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4141 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4201 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4261 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4321 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     4381 ttgacgagtt cttctgaatt attaacgctt acaatttcct gatgcggtat tttctcctta
     4441 cgcatctgtg cggtatttca caccgcatac aggtggcact tttcggggaa atgtgcgcgg
     4501 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4561 accctgataa atgcttcaat aatagcacgt gaggagggcc accatggcca agttgaccag
     4621 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
     4681 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
     4741 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
     4801 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
     4861 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
     4921 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
     4981 cgtgctaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5041 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5101 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5161 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5221 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5281 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5341 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5401 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5461 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5521 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5581 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5641 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5701 gaaaaacgcc agcaacgcgg cctttttacg gttcctgggc ttttgctggc cttttgctca
     5761 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5821 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5881 ggaag
//
