LOCUS       Exported               12685 bp ds-DNA     circular SYN 23-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 23, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12685
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    454..634
                     /locus_tag="HIV-1_5_LTR"
                     /label=HIV-1_5_LTR
                     /note="color: #00ffff"
     misc_feature    454..634
                     /locus_tag="HIV-1_Trunc_3_LTR"
                     /label=HIV-1_Trunc_3_LTR
                     /note="color: #8000ff"
     misc_feature    745..789
                     /locus_tag="HIV-1 Psi"
                     /label=HIV-1 Psi
                     /note="color: #0080ff"
     misc_feature    790..1251
                     /locus_tag="HIV-1 Gag"
                     /label=HIV-1 Gag
                     /note="color: #80ff80"
     misc_feature    1803..2036
                     /locus_tag="RRE"
                     /label=RRE
                     /note="color: #004000"
     CDS             2221..2265
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2559..2588
                     /locus_tag="HIV_Rev_NES"
                     /label=HIV_Rev_NES
                     /note="color: #804000"
     CDS             2559..2585
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /note="color: #993366"
                     /translation="LPPLERLTL"
     misc_feature    2851..3403
                     /locus_tag="CMV_immearly_promoter(1)"
                     /label=CMV_immearly_promoter
                     /label=CMV_immearly_promoter(1)
                     /note="color: #800080"
     misc_feature    2851..3184
                     /locus_tag="CMV_immearly_promoter"
                     /label=CMV_immearly_promoter
                     /note="color: #800080"
     enhancer        2902..3205
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        3185..3428
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="color: #6495ed"
     misc_feature    join(3185..3239,3241..3404)
                     /locus_tag="CMV Variant"
                     /label=CMV Variant
                     /note="This feature has 2 segments:
                        1: 3185 .. 3239 / #5fa061
                        2: 3241 .. 3404 / #5fa061"
     misc_feature    3185..3403
                     /locus_tag="CMV Prom Var"
                     /label=CMV Prom Var
                     /note="color: #00ffff"
     promoter        3206..3409
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    3361..3430
                     /locus_tag="CMV_promoter"
                     /label=CMV_promoter
                     /note="color: #408080"
     CDS             3446..4153
                     /locus_tag="mCherry"
                     /label=mCherry
                     /note="color: #ff5558; direction: RIGHT"
     CDS             4178..4231
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 4228"
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    4232..4948
                     /locus_tag="Mod_GFP"
                     /label=Mod_GFP
                     /note="color: #00ff00"
     misc_feature    4507..4509
                     /locus_tag="TCA motif"
                     /label=TCA motif
                     /note="color: #ff80c0"
     misc_feature    4520..4522
                     /locus_tag="Sp PAM"
                     /label=Sp PAM
                     /note="color: #ff80c0"
     misc_feature    4983..5556
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     misc_feature    4994..5545
                     /locus_tag="IRES"
                     /label=IRES
                     /note="color: #74ae51"
     misc_feature    5550..6614
                     /locus_tag="Hygro-1"
                     /label=Hygro-1
                     /note="color: #408080"
     misc_feature    5550..6614
                     /locus_tag="Hygro"
                     /label=Hygro
                     /note="color: #5c98a3"
     CDS             5595..6614
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_RNA        6774..6849
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
     misc_feature    6920..6941
                     /locus_tag="U3PPT"
                     /label=U3PPT
                     /note="color: #80ff80"
     LTR             6937..7570
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    7390..7570
                     /locus_tag="HIV-1_5_LTR-2"
                     /label=HIV-1_5_LTR-2
                     /note="color: #008040"
     rep_origin      complement(9362..10044)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
                     /note="color: #808080"
     rep_origin      complement(9380..9968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10139..10999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10139 .. 10930 / #ccffcc
                        2: 10931 .. 10999 / #ccffcc / signal sequence
                     Cleavage site after base 10930"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(10142..10801)
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="color: #ffff00; direction: LEFT"
     promoter        complement(11000..11104)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     CDS             complement(11438..11506)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
                     /note="color: #6495ed; direction: LEFT"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atcgacatag
     1261 cagaataggc gttactcgac agaggagagc aagaaatgga gccagtagat cctagactag
     1321 agccctggaa gcatccagga agtcagccta aaactgcttg taccaattgc tattgtaaaa
     1381 agtgttgctt tcattgccaa gtttgtttca tgacaaaagc cttaggcatc tcctatggca
     1441 ggaagaagcg gagacagcga cgaagagctc atcagaacag tcagactcat caagcttctc
     1501 tatcaaagca gtaagtagta catgggcgcg cccatgtggc aggaagtagg aaaagcaatg
     1561 tatgcccctc ccatcagtgg acaaattaga tgttcatcaa atattactgg gctgctatta
     1621 acaagagatg gtggtaataa caacaatggg tccgagatct tcagacctgg aggaggcgat
     1681 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1741 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1801 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1861 atgacgctga cggtacaggc cagacaatta ttgtctgata tagtgcagca gcagaacaat
     1921 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaaa
     1981 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2041 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2101 agtaataaat ctctggaaca gatttggaat aacatgacct ggatggagtg ggacagagaa
     2161 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2221 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2281 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2341 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2401 ttatcgtttc agacccacct cccaatcccg aggggacccg acaggcccga aggaatagaa
     2461 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atccttagca
     2521 cttatctggg acgatctgcg gagcctgtgc ctcttcagct accaccgctt gagagactta
     2581 ctcttgattg taacgaggat tgtggaactt ctgggacgca gggggtggga agccctcaaa
     2641 tattggtgga atctcctaca atattggagt caggagctaa agaatagtgc tgttagcttg
     2701 ctcaatgcca cagccatagc agtagctgag gggacagata gggttataga agtagtacaa
     2761 ggagcttcta gagctattcg ccacatacct agaagaataa gacagggctt ggaaaggatt
     2821 ttgctataag atgggtggcg cggccgctta atagtaatca attacggggt cattagttca
     2881 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2941 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     3001 tgggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     3061 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     3121 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     3181 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     3241 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     3301 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     3361 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
     3421 ccgtcagatc cagctggcta ccggtatggt gagcaagggc gaggaggata acatggccat
     3481 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     3541 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3601 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     3661 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     3721 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     3781 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     3841 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     3901 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     3961 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     4021 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     4081 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     4141 cgagctgtac aagcccggtg cagatctcga gtacaaggag ggcagaggaa gtcttctaac
     4201 atgcggtgac gtggaggaga atcccggccc tatggtcagt aaaggtgaag aactgttcac
     4261 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     4321 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     4381 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     4441 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     4501 cgaaggccac gtccaggagc ggaccatctt cttcaaggac gacggcaact acaagacccg
     4561 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4621 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4681 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     4741 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4801 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     4861 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4921 cactctcggt atggatgaat tatataaata acgtacgctt aattaacgga tccggaattc
     4981 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5041 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5101 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5161 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5221 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5281 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5341 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtagtcaac
     5401 aaggggctga aggatgccca gaaggtaccc cattgtatgg gaatctgatc tggggcctcg
     5461 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
     5521 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc gtaccaaaga
     5581 tggatagatc cggaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     5641 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     5701 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     5761 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     5821 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     5881 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     5941 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     6001 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     6061 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     6121 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     6181 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     6241 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     6301 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     6361 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     6421 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     6481 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     6541 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     6601 gggcaaagga atagagtaga tgccgaccga acaaggcatg ctcttcaacc tcaataacgt
     6661 tatatcctga ttcacgtaat atttttgggg taaatttagt tcctgttcca ttaactgcgc
     6721 taaaaataat ttttaaatct tttttagctt cttgctcttt tttgtacgtc tctgttttag
     6781 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     6841 agtcggtgct tttttgaatt cgttcggtac ctttaagacc aatgacttac aaggcagctg
     6901 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaaa
     6961 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     7021 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     7081 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     7141 tacaccctgt gagcctgcat ggaatggatg accctgagag agaagtgtta gagtggaggt
     7201 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     7261 actgctgaca tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     7321 gcctgggcgg gactggggag tggcgagccc tcagatgctg catataagca gctgcttttt
     7381 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7441 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7501 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7561 atctctagca cccaggaggt agaggttgca gtgagccaag atcgcgccac tgcattccag
     7621 cctgggcaag aaaacaagac tgtctaaaat aataataata agttaagggt attaaatata
     7681 tttatacatg gaggtcataa aaatatatat atttgggctg ggcgcagtgg ctcacacctg
     7741 cgcccggccc tttgggaggc cgaggcaggt ggatcacctg agtttgggag ttccagacca
     7801 gcctgaccaa catggagaaa ccccttctct gtgtattttt agtagatttt attttatgtg
     7861 tattttattc acaggtattt ctggaaaact gaaactgttt ttcctctact ctgataccac
     7921 aagaatcatc agcacagagg aagacttctg tgatcaaatg tggtgggaga gggaggtttt
     7981 caccagcaca tgagcagtca gttctgccgc agactcggcg ggtgtccttc ggttcagttc
     8041 caacaccgcc tgcctggaga gaggtcagac cacagggtga gggctcagtc cccaagacat
     8101 aaacacccaa gacataaaca cccaacaggt ccaccccgcc tgctgcccag gcagagccga
     8161 ttcaccaaga cgggaattag gatagagaaa gagtaagtca cacagagccg gctgtgcggg
     8221 agaacggagt tctattatga ctcaaatcag tctccccaag cattcgggga tcagagtttt
     8281 taaggataac ttagtgtgta gggggccagt gagttggaga tgaaagcgta gggagtcgaa
     8341 ggtgtccttt tgcgccgagt cagttcctgg gtgggggcca caagatcgga tgagccagtt
     8401 tatcaatccg ggggtgccag ctgatccatg gagtgcaggg tctgcaaaat atctcaagca
     8461 ctgattgatc ttaggtttta caatagtgat gttaccccag gaacaatttg gggaaggtca
     8521 gaatcttgta gcctgtagct gcatgactcc taaaccataa tttctttttt gttttttttt
     8581 ttttattttt gagacagggt ctcactctgt cacctaggct ggagtgcagt ggtgcaatca
     8641 cagctcactg cagcctcaac gtcgtaagct caagcgatcc tcccacctca gcctgcctgg
     8701 tagctgagac tacaagcgac gccccagtta atttttgtat ttttggtaga ggcagcgttt
     8761 tgccgtgtgg ccctggctgg tctcgaactc ctgggctcaa gtgatccagc ctcagcctcc
     8821 caaagtgctg ggacaaccgg gcccagtcac tgcacctggc cctaaaccat aatttctaat
     8881 cttttggcta atttgttagt cctacaaagg cagtctagtc cccagcaaaa agggggtttg
     8941 tttcgggaaa gggctgttac tgtctttgtt tcaaactata aactaagttc ctcctaaact
     9001 tagttcggcc tacacccagg aatgaacaag gagagcttgg aggttagaag cacgatggaa
     9061 ttggttaggt cagatctctt tcactgtctg agttataatt ttgcaatggt ggttcaaaga
     9121 ctgcccgctt ctgacaccag tcgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9181 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     9241 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     9301 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     9361 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     9421 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9481 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     9541 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9601 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9661 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9721 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9781 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     9841 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9901 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9961 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10021 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10081 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10141 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10201 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    10261 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    10321 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    10381 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    10441 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    10501 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    10561 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10621 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10681 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10741 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10801 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10861 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10921 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10981 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11041 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11101 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    11161 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
    11221 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    11281 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
    11341 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    11401 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    11461 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagggg aggcagagat
    11521 tgcagtaagc tgagatcgca gcactgcact ccagcctggg cgacagagta agactctgtc
    11581 tcaaaaataa aataaataaa tcaatcagat attccaatct tttcctttat ttatttattt
    11641 attttctatt ttggaaacac agtccttcct tattccagaa ttacacatat attctatttt
    11701 tctttatatg ctccagtttt ttttagacct tcacctgaaa tgtgtgtata caaaatctag
    11761 gccagtccag cagagcctaa aggtaaaaaa taaaataata aaaaataaat aaaatctagc
    11821 tcactccttc acatcaaaat ggagatacag ctgttagcat taaataccaa ataacccatc
    11881 ttgtcctcaa taattttaag cgcctctctc caccacatct aactcctgtc aaaggcatgt
    11941 gccccttccg ggcgctctgc tgtgctgcca accaactggc atgtggactc tgcagggtcc
    12001 ctaactgcca agccccacag tgtgccctga ggctgcccct tccttctagc ggctgccccc
    12061 actcggcttt gctttcccta gtttcagtta cttgcgttca gccaaggtct gaaactaggt
    12121 gcgcacagag cggtaagact gcgagagaaa gagaccagct ttacaggggg tttatcacag
    12181 tgcaccctga cagtcgtcag cctcacaggg ggtttatcac attgcaccct gacagtcgtc
    12241 agcctcacag ggggtttatc acagtgcacc cttacaatca ttccatttga ttcacaattt
    12301 ttttagtctc tactgtgcct aacttgtaag ttaaatttga tcagaggtgt gttcccagag
    12361 gggaaaacag tatatacagg gttcagtact atcgcatttc aggcctccac ctgggtcttg
    12421 gaatgtgtcc cccgaggggt gatgactacc tcagttggat ctccacaggt cacagtgaca
    12481 caagataacc aagacacctc ccaaggctac cacaatgggc cgccctccac gtgcacatgg
    12541 ccggaggaac tgccatgtcg gaggtgcaag cacacctgcg catcagagtc cttggtgtgg
    12601 agggagggac cagcgcagct tccagccatc cacctgatga acagaaccta gggaaagccc
    12661 cagttctact tacaccagga aaggc
//
