LOCUS       Exported                9294 bp ds-DNA     circular SYN 23-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 23, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..223
                     /locus_tag="CMV Start"
                     /label=CMV Start
                     /note="color: #48ff68"
     misc_feature    224..575
                     /locus_tag="CMV Continued"
                     /label=CMV Continued
                     /note="color: #64ff78"
     promoter        355..558
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      603..612
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     misc_feature    612..755
                     /locus_tag="A3B start"
                     /label=A3B start
                     /note="color: #ff80ff"
     misc_feature    756..1657
                     /locus_tag="L1 intron"
                     /label=L1 intron
                     /note="color: #8e92ff"
     misc_feature    1658..2080
                     /locus_tag="A3B exon6 no stop"
                     /label=A3B exon6 no stop
                     /note="color: #ff80ff"
     primer_bind     1862..1888
                     /label=D131E
                     /note="color: black; sequence: GCCCGCATCTATGAgTACGACCCCCTA"
     misc_feature    1877..1894
                     /locus_tag="loop 7"
                     /label=loop 7
                     /note="color: #ff80c0"
     misc_feature    2081..2128
                     /locus_tag="XTEN Linker"
                     /label=XTEN Linker
                     /note="color: #dd9dfa"
     misc_feature    2129..6229
                     /locus_tag="Cas9"
                     /label=Cas9
                     /note="color: #5ff8fa"
     CDS             2129..6229
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /note="This feature has 3 segments:
                        1: 2129 .. 2152 / #993366
                        2: 2153 .. 2155 / #cc99b2
                        3: 2156 .. 6229 / #993366"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    6242..6490
                     /locus_tag="UGI"
                     /label=UGI
                     /note="color: #f8686a"
     CDS             6242..6490
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /note="color: #993366"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     misc_feature    6503..6523
                     /locus_tag="SV40 NLS"
                     /label=SV40 NLS
                     /note="color: #fad816"
     CDS             6503..6523
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             6532..6549
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     polyA_signal    6578..6802
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6873..6889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6897..6913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6921..6951)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6921 .. 6927 / #ffffff / -10
                        2: 6928 .. 6945 / #ffffff
                        3: 6946 .. 6951 / #ffffff / -35"
     protein_bind    6966..6987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(7275..7863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(8034..8894)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8034 .. 8825 / #ccffcc
                        2: 8826 .. 8894 / #ccffcc / signal sequence
                     Cleavage site after base 8825"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8895..8999)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     enhancer        join(9269..9294,1..354)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
ORIGIN
        1 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
       61 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      121 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      181 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      241 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      301 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      361 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      421 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      481 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      541 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagagatc cgcggccgcg
      601 ccgccaccat ggatccagac acattcactt tcaactttaa taatgaccct ttggtccttc
      661 gacggcgcca gacctacttg tgctatgagg tggagcgcct ggacaatggc acctgggtcc
      721 tgatggacca gcacatgggc tttctatgca acgaggtgag tccaggagat gtttcagcac
      781 tgttgccttt agtctcgagg caacttagac aactgagtat tgatctgagc acagcagggt
      841 gtgagctgtt tgaagatact ggggttgggg gtgaagaaac tgcagaggac taactgggct
      901 gagacccagt ggcaatgttt tagggcctaa ggaatgcctc tgaaaatcta gatggacaac
      961 tttgactttg agaaaagaga ggtggaaatg aggaaaatga cttttcttta ttagatttcg
     1021 gtagaaagaa ctttcatctt tcccctattt ttgttattcg ttttaaaaca tctatctgga
     1081 ggcaggacaa gtatggtcat taaaaagatg caggcagaag gcatatattg gctcagtcaa
     1141 agtggggaac tttggtggcc aaacatacat tgctaaggct attcctatat cagctggaca
     1201 catataaaat gctgctaatg cttcattaca aacttatatc ctttaattcc agatgggggc
     1261 aaagtatgtc caggggtgag gaacaattga aacatttggg ctggagtaga ttttgaaagt
     1321 cagctctgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgcgcgcatg tgtgtttgtg
     1381 tgtgtgtgag agcgtgtgtt tcttttaacg ttttcagcct acagcataca gggttcatgg
     1441 tggcaagaag ataacaagat ttaaattatg gccagtgact agtgctgcaa gaagaacaac
     1501 tacctgcatt taatgggaaa gcaaaatctc aggctttgag ggaagttaac ataggcttga
     1561 ttctgggtgg aagctgggtg tgtagttatc tggaggccag gctggagctc tcagctcact
     1621 atgggttcat ctttattgtc tcctttcatc tcaacaggct aagaatcttc tctgtggctt
     1681 ttacggccgc catgcggagc tgcgcttctt ggacctggtt ccttctttgc agttggaccc
     1741 ggcccagatc tacagggtca cttggttcat ctcctggagc ccctgcttct cctggggctg
     1801 tgccggggaa gtgcgtgcgt tccttcagga gaacacacac gtgagactgc gcatcttcgc
     1861 tgcccgcatc tatgagtacg accccctata taaggaggcg ctgcaaatgc tgcgggatgc
     1921 tggggcccaa gtctccatca tgacctacga tgagtttgag tactgctggg acacctttgt
     1981 gtaccgccag ggatgtccct tccagccctg ggatggacta gaggagcaca gccaagccct
     2041 gagtgggagg ctgcgggcca ttctccagaa tcagggaaac agcggcagcg agactcccgg
     2101 gacctcagag tccgccacac ccgaaagtga taaaaagtat tctattggtt tagccatcgg
     2161 cactaattcc gttggatggg ctgtcataac cgatgaatac aaagtacctt caaagaaatt
     2221 taaggtgttg gggaacacag accgtcattc gattaaaaag aatcttatcg gtgccctcct
     2281 attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa cgaaccgctc ggagaaggta
     2341 tacacgtcgc aagaaccgaa tatgttactt acaagaaatt tttagcaatg agatggccaa
     2401 agttgacgat tctttctttc accgtttgga agagtccttc cttgtcgaag aggacaagaa
     2461 acatgaacgg caccccatct ttggaaacat agtagatgag gtggcatatc atgaaaagta
     2521 cccaacgatt tatcacctca gaaaaaagct agttgactca actgataaag cggacctgag
     2581 gttaatctac ttggctcttg cccatatgat aaagttccgt gggcactttc tcattgaggg
     2641 tgatctaaat ccggacaact cggatgtcga caaactgttc atccagttag tacaaaccta
     2701 taatcagttg tttgaagaga accctataaa tgcaagtggc gtggatgcga aggctattct
     2761 tagcgcccgc ctctctaaat cccgacggct agaaaacctg atcgcacaat tacccggaga
     2821 gaagaaaaat gggttgttcg gtaaccttat agcgctctca ctaggcctga caccaaattt
     2881 taagtcgaac ttcgacttag ctgaagatgc caaattgcag cttagtaagg acacgtacga
     2941 tgacgatctc gacaatctac tggcacaaat tggagatcag tatgcggact tatttttggc
     3001 tgccaaaaac cttagcgatg caatcctcct atctgacata ctgagagtta atactgagat
     3061 taccaaggcg ccgttatccg cttcaatgat caaaaggtac gatgaacatc accaagactt
     3121 gacacttctc aaggccctag tccgtcagca actgcctgag aaatataagg aaatattctt
     3181 tgatcagtcg aaaaacgggt acgcaggtta tattgacggc ggagcgagtc aagaggaatt
     3241 ctacaagttt atcaaaccca tattagagaa gatggatggg acggaagagt tgcttgtaaa
     3301 actcaatcgc gaagatctac tgcgaaagca gcggactttc gacaacggta gcattccaca
     3361 tcaaatccac ttaggcgaat tgcatgctat acttagaagg caggaggatt tttatccgtt
     3421 cctcaaagac aatcgtgaaa agattgagaa aatcctaacc tttcgcatac cttactatgt
     3481 gggacccctg gcccgaggga actctcggtt cgcatggatg acaagaaagt ccgaagaaac
     3541 gattactcca tggaattttg aggaagttgt cgataaaggt gcgtcagctc aatcgttcat
     3601 cgagaggatg accaactttg acaagaattt accgaacgaa aaagtattgc ctaagcacag
     3661 tttactttac gagtatttca cagtgtacaa tgaactcacg aaagttaagt atgtcactga
     3721 gggcatgcgt aaacccgcct ttctaagcgg agaacagaag aaagcaatag tagatctgtt
     3781 attcaagacc aaccgcaaag tgacagttaa gcaattgaaa gaggactact ttaagaaaat
     3841 tgaatgcttc gattctgtcg agatctccgg ggtagaagat cgatttaatg cgtcacttgg
     3901 tacgtatcat gacctcctaa agataattaa agataaggac ttcctggata acgaagagaa
     3961 tgaagatatc ttagaagata tagtgttgac tcttaccctc tttgaagatc gggaaatgat
     4021 tgaggaaaga ctaaaaacat acgctcacct gttcgacgat aaggttatga aacagttaaa
     4081 gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa cttatcaacg ggataagaga
     4141 caagcaaagt ggtaaaacta ttctcgattt tctaaagagc gacggcttcg ccaataggaa
     4201 ctttatgcag ctgatccatg atgactcttt aaccttcaaa gaggatatac aaaaggcaca
     4261 ggtttccgga caaggggact cattgcacga acatattgcg aatcttgctg gttcgccagc
     4321 catcaaaaag ggcatactcc agacagtcaa agtagtggat gagctagtta aggtcatggg
     4381 acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc gaaaatcaaa cgactcagaa
     4441 ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa gagggtatta aagaactggg
     4501 cagccagatc ttaaaggagc atcctgtgga aaatacccaa ttgcagaacg agaaacttta
     4561 cctctattac ctacaaaatg gaagggacat gtatgttgat caggaactgg acataaaccg
     4621 tttatctgat tacgacgtcg atcacattgt accccaatcc tttttgaagg acgattcaat
     4681 cgacaataaa gtgcttacac gctcggataa gaaccgaggg aaaagtgaca atgttccaag
     4741 cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag ctcctaaatg cgaaactgat
     4801 aacgcaaaga aagttcgata acttaactaa agctgagagg ggtggcttgt ctgaacttga
     4861 caaggccgga tttattaaac gtcagctcgt ggaaacccgc caaatcacaa agcatgttgc
     4921 acagatacta gattcccgaa tgaatacgaa atacgacgag aacgataagc tgattcggga
     4981 agtcaaagta atcactttaa agtcaaaatt ggtgtcggac ttcagaaagg attttcaatt
     5041 ctataaagtt agggagataa ataactacca ccatgcgcac gacgcttatc ttaatgccgt
     5101 cgtagggacc gcactcatta agaaataccc gaagctagaa agtgagtttg tgtatggtga
     5161 ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc gaacaggaga taggcaaggc
     5221 tacagccaaa tacttctttt attctaacat tatgaatttc tttaagacgg aaatcactct
     5281 ggcaaacgga gagatacgca aacgaccttt aattgaaacc aatggggaga caggtgaaat
     5341 cgtatgggat aagggccggg acttcgcgac ggtgagaaaa gttttgtcca tgccccaagt
     5401 caacatagta aagaaaactg aggtgcagac cggagggttt tcaaaggaat cgattcttcc
     5461 aaaaaggaat agtgataagc tcatcgctcg taaaaaggac tgggacccga aaaagtacgg
     5521 tggcttcgat agccctacag ttgcctattc tgtcctagta gtggcaaaag ttgagaaggg
     5581 aaaatccaag aaactgaagt cagtcaaaga attattgggg ataacgatta tggagcgctc
     5641 gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa ggttacaagg aagtaaaaaa
     5701 ggatctcata attaaactac caaagtatag tctgtttgag ttagaaaatg gccgaaaacg
     5761 gatgttggct agcgccggag agcttcaaaa ggggaacgaa ctcgcactac cgtctaaata
     5821 cgtgaatttc ctgtatttag cgtcccatta cgagaagttg aaaggttcac ctgaagataa
     5881 cgaacagaag caactttttg ttgagcagca caaacattat ctcgacgaaa tcatagagca
     5941 aatttcggaa ttcagtaaga gagtcatcct agctgatgcc aatctggaca aagtattaag
     6001 cgcatacaac aagcacaggg ataaacccat acgtgagcag gcggaaaata ttatccattt
     6061 gtttactctt accaacctcg gcgctccagc cgcattcaag tattttgaca caacgataga
     6121 tcgcaaacga tacacttcta ccaaggaggt gctagacgcg acactgattc accaatccat
     6181 cacgggatta tatgaaactc ggatagattt gtcacagctt gggggtgact ctggtggttc
     6241 tactaatctg tcagatatta ttgaaaagga gaccggtaag caactggtta tccaggaatc
     6301 catcctcatg ctcccagagg aggtggaaga agtcattggg aacaagccgg aaagcgatat
     6361 actcgtgcac accgcctacg acgagagcac cgacgagaat gtcatgcttc tgactagcga
     6421 cgcccctgaa tacaagcctt gggctctggt catacaggat agcaacggtg agaacaagat
     6481 taagatgctc tctggtggtt ctcccaagaa gaagaggaaa gtctaaccgg tcatcatcac
     6541 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     6601 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6661 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6721 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6781 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg
     6841 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6901 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt
     6961 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7021 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7081 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7681 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8341 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8641 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9001 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc tcccgatccc
     9061 ctagggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     9121 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     9181 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     9241 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaa
//
