LOCUS       Exported                8386 bp ds-DNA     circular SYN 23-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Feb 23, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..352
                     /locus_tag="CMV Continued"
                     /label=CMV Continued
                     /note="color: #ffff80"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     regulatory      380..389
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     misc_feature    401..949
                     /locus_tag="AID 1-188"
                     /label=AID 1-188
                     /note="color: #ff8080"
     misc_feature    950..965
                     /locus_tag="XTEN Linker(1)"
                     /label=XTEN Linker
                     /label=XTEN Linker(1)
                     /note="color: #00ff80"
     misc_feature    966..997
                     /locus_tag="XTEN Linker"
                     /label=XTEN Linker
                     /note="color: #00ff80"
     misc_feature    998..5098
                     /locus_tag="Cas9"
                     /label=Cas9
                     /note="color: #8fc7f3"
     CDS             998..5098
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /note="This feature has 3 segments:
                        1:  998 .. 1021 / #993366
                        2: 1022 .. 1024 / #cc99b2
                        3: 1025 .. 5098 / #993366"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    5111..5359
                     /locus_tag="UGI"
                     /label=UGI
                     /note="color: #80ffff"
     CDS             5111..5359
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /note="color: #993366"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     misc_feature    5372..5392
                     /locus_tag="SV40 NLS"
                     /label=SV40 NLS
                     /note="color: #80ff80"
     CDS             5372..5392
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             5401..5418
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     polyA_signal    5447..5671
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(5742..5758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    5766..5782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5790..5820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5790 .. 5796 / #ffffff / -10
                        2: 5797 .. 5814 / #ffffff
                        3: 5815 .. 5820 / #ffffff / -35"
     protein_bind    5835..5856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6144..6732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(6903..7763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6903 .. 7694 / #ccffcc
                        2: 7695 .. 7763 / #ccffcc / signal sequence
                     Cleavage site after base 7694"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7764..7868)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     enhancer        join(8138..8386,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     misc_feature    8164..8386
                     /locus_tag="CMV Start"
                     /label=CMV Start
                     /note="color: #ffff80"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgccg ccaccatgga cagcctcttg atgaaccgga ggaagtttct
      421 ttaccaattc aaaaatgtcc gctgggctaa gggtcggcgt gagacctacc tgtgctacgt
      481 agtgaagagg cgtgacagtg ctacatcctt ttcactggac tttggttatc ttcgcaataa
      541 gaacggctgc cacgtggaat tgctcttcct ccgctacatc tcggactggg acctagaccc
      601 tggccgctgc taccgcgtca cctggttcac ctcctggagc ccctgctacg actgtgcccg
      661 acatgtggcc gactttctgc gagggaaccc caacctcagt ctgaggatct tcaccgcgcg
      721 cctctacttc tgtgaggacc gcaaggctga gcccgagggg ctgcggcggc tgcaccgcgc
      781 cggggtgcaa atagccatca tgaccttcaa agattatttt tactgctgga atacttttgt
      841 agaaaaccat gaaagaactt tcaaagcctg ggaagggctg catgaaaatt cagttcgtct
      901 ctccagacag cttcggcgca tccttttgcc cctgtatgag gttgatgaca gcggcagcga
      961 gactcccggg acctcagagt ccgccacacc cgaaagtgat aaaaagtatt ctattggttt
     1021 agccatcggc actaattccg ttggatgggc tgtcataacc gatgaataca aagtaccttc
     1081 aaagaaattt aaggtgttgg ggaacacaga ccgtcattcg attaaaaaga atcttatcgg
     1141 tgccctccta ttcgatagtg gcgaaacggc agaggcgact cgcctgaaac gaaccgctcg
     1201 gagaaggtat acacgtcgca agaaccgaat atgttactta caagaaattt ttagcaatga
     1261 gatggccaaa gttgacgatt ctttctttca ccgtttggaa gagtccttcc ttgtcgaaga
     1321 ggacaagaaa catgaacggc accccatctt tggaaacata gtagatgagg tggcatatca
     1381 tgaaaagtac ccaacgattt atcacctcag aaaaaagcta gttgactcaa ctgataaagc
     1441 ggacctgagg ttaatctact tggctcttgc ccatatgata aagttccgtg ggcactttct
     1501 cattgagggt gatctaaatc cggacaactc ggatgtcgac aaactgttca tccagttagt
     1561 acaaacctat aatcagttgt ttgaagagaa ccctataaat gcaagtggcg tggatgcgaa
     1621 ggctattctt agcgcccgcc tctctaaatc ccgacggcta gaaaacctga tcgcacaatt
     1681 acccggagag aagaaaaatg ggttgttcgg taaccttata gcgctctcac taggcctgac
     1741 accaaatttt aagtcgaact tcgacttagc tgaagatgcc aaattgcagc ttagtaagga
     1801 cacgtacgat gacgatctcg acaatctact ggcacaaatt ggagatcagt atgcggactt
     1861 atttttggct gccaaaaacc ttagcgatgc aatcctccta tctgacatac tgagagttaa
     1921 tactgagatt accaaggcgc cgttatccgc ttcaatgatc aaaaggtacg atgaacatca
     1981 ccaagacttg acacttctca aggccctagt ccgtcagcaa ctgcctgaga aatataagga
     2041 aatattcttt gatcagtcga aaaacgggta cgcaggttat attgacggcg gagcgagtca
     2101 agaggaattc tacaagttta tcaaacccat attagagaag atggatggga cggaagagtt
     2161 gcttgtaaaa ctcaatcgcg aagatctact gcgaaagcag cggactttcg acaacggtag
     2221 cattccacat caaatccact taggcgaatt gcatgctata cttagaaggc aggaggattt
     2281 ttatccgttc ctcaaagaca atcgtgaaaa gattgagaaa atcctaacct ttcgcatacc
     2341 ttactatgtg ggacccctgg cccgagggaa ctctcggttc gcatggatga caagaaagtc
     2401 cgaagaaacg attactccat ggaattttga ggaagttgtc gataaaggtg cgtcagctca
     2461 atcgttcatc gagaggatga ccaactttga caagaattta ccgaacgaaa aagtattgcc
     2521 taagcacagt ttactttacg agtatttcac agtgtacaat gaactcacga aagttaagta
     2581 tgtcactgag ggcatgcgta aacccgcctt tctaagcgga gaacagaaga aagcaatagt
     2641 agatctgtta ttcaagacca accgcaaagt gacagttaag caattgaaag aggactactt
     2701 taagaaaatt gaatgcttcg attctgtcga gatctccggg gtagaagatc gatttaatgc
     2761 gtcacttggt acgtatcatg acctcctaaa gataattaaa gataaggact tcctggataa
     2821 cgaagagaat gaagatatct tagaagatat agtgttgact cttaccctct ttgaagatcg
     2881 ggaaatgatt gaggaaagac taaaaacata cgctcacctg ttcgacgata aggttatgaa
     2941 acagttaaag aggcgtcgct atacgggctg gggacgattg tcgcggaaac ttatcaacgg
     3001 gataagagac aagcaaagtg gtaaaactat tctcgatttt ctaaagagcg acggcttcgc
     3061 caataggaac tttatgcagc tgatccatga tgactcttta accttcaaag aggatataca
     3121 aaaggcacag gtttccggac aaggggactc attgcacgaa catattgcga atcttgctgg
     3181 ttcgccagcc atcaaaaagg gcatactcca gacagtcaaa gtagtggatg agctagttaa
     3241 ggtcatggga cgtcacaaac cggaaaacat tgtaatcgag atggcacgcg aaaatcaaac
     3301 gactcagaag gggcaaaaaa acagtcgaga gcggatgaag agaatagaag agggtattaa
     3361 agaactgggc agccagatct taaaggagca tcctgtggaa aatacccaat tgcagaacga
     3421 gaaactttac ctctattacc tacaaaatgg aagggacatg tatgttgatc aggaactgga
     3481 cataaaccgt ttatctgatt acgacgtcga tcacattgta ccccaatcct ttttgaagga
     3541 cgattcaatc gacaataaag tgcttacacg ctcggataag aaccgaggga aaagtgacaa
     3601 tgttccaagc gaggaagtcg taaagaaaat gaagaactat tggcggcagc tcctaaatgc
     3661 gaaactgata acgcaaagaa agttcgataa cttaactaaa gctgagaggg gtggcttgtc
     3721 tgaacttgac aaggccggat ttattaaacg tcagctcgtg gaaacccgcc aaatcacaaa
     3781 gcatgttgca cagatactag attcccgaat gaatacgaaa tacgacgaga acgataagct
     3841 gattcgggaa gtcaaagtaa tcactttaaa gtcaaaattg gtgtcggact tcagaaagga
     3901 ttttcaattc tataaagtta gggagataaa taactaccac catgcgcacg acgcttatct
     3961 taatgccgtc gtagggaccg cactcattaa gaaatacccg aagctagaaa gtgagtttgt
     4021 gtatggtgat tacaaagttt atgacgtccg taagatgatc gcgaaaagcg aacaggagat
     4081 aggcaaggct acagccaaat acttctttta ttctaacatt atgaatttct ttaagacgga
     4141 aatcactctg gcaaacggag agatacgcaa acgaccttta attgaaacca atggggagac
     4201 aggtgaaatc gtatgggata agggccggga cttcgcgacg gtgagaaaag ttttgtccat
     4261 gccccaagtc aacatagtaa agaaaactga ggtgcagacc ggagggtttt caaaggaatc
     4321 gattcttcca aaaaggaata gtgataagct catcgctcgt aaaaaggact gggacccgaa
     4381 aaagtacggt ggcttcgata gccctacagt tgcctattct gtcctagtag tggcaaaagt
     4441 tgagaaggga aaatccaaga aactgaagtc agtcaaagaa ttattgggga taacgattat
     4501 ggagcgctcg tcttttgaaa agaaccccat cgacttcctt gaggcgaaag gttacaagga
     4561 agtaaaaaag gatctcataa ttaaactacc aaagtatagt ctgtttgagt tagaaaatgg
     4621 ccgaaaacgg atgttggcta gcgccggaga gcttcaaaag gggaacgaac tcgcactacc
     4681 gtctaaatac gtgaatttcc tgtatttagc gtcccattac gagaagttga aaggttcacc
     4741 tgaagataac gaacagaagc aactttttgt tgagcagcac aaacattatc tcgacgaaat
     4801 catagagcaa atttcggaat tcagtaagag agtcatccta gctgatgcca atctggacaa
     4861 agtattaagc gcatacaaca agcacaggga taaacccata cgtgagcagg cggaaaatat
     4921 tatccatttg tttactctta ccaacctcgg cgctccagcc gcattcaagt attttgacac
     4981 aacgatagat cgcaaacgat acacttctac caaggaggtg ctagacgcga cactgattca
     5041 ccaatccatc acgggattat atgaaactcg gatagatttg tcacagcttg ggggtgactc
     5101 tggtggttct actaatctgt cagatattat tgaaaaggag accggtaagc aactggttat
     5161 ccaggaatcc atcctcatgc tcccagagga ggtggaagaa gtcattggga acaagccgga
     5221 aagcgatata ctcgtgcaca ccgcctacga cgagagcacc gacgagaatg tcatgcttct
     5281 gactagcgac gcccctgaat acaagccttg ggctctggtc atacaggata gcaacggtga
     5341 gaacaagatt aagatgctct ctggtggttc tcccaagaag aagaggaaag tctaaccggt
     5401 catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt
     5461 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5521 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5581 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5641 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     5701 tcgataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5761 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5821 gcctagggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5881 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5941 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6001 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6061 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6121 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6181 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6241 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6301 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6361 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6421 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6481 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6541 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6601 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6661 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6721 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6781 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6841 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6901 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6961 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7021 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7081 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7141 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7201 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7261 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7321 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7381 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7441 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7501 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7561 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7621 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7681 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7741 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7801 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7861 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct
     7921 cccgatcccc tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag
     7981 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     8041 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     8101 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     8161 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     8221 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     8281 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     8341 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatc
//
