LOCUS       Exported                2681 bp DNA     circular SYN 24-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ariel
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 24, 2023 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      66..113
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(131..946)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1068..1656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1684..1702
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1734..1756
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1764..1778
                     /locus_tag="CAT(1)"
                     /label=CAT
     misc_feature    1779..1805
                     /locus_tag="Strep"
                     /label=Strep
     misc_feature    1806..1814
                     /locus_tag="Linker Region"
                     /label=Linker Region
     misc_feature    1815..2678
                     /locus_tag="CSTI"
                     /label=CSTI
ORIGIN
        1 gtcgaccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
       61 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      121 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
      181 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
      241 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
      301 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
      361 gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt
      421 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
      481 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
      541 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
      601 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
      661 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
      721 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
      781 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
      841 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
      901 ttaatcgcgg cttcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
      961 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     1021 tgtaacatca gagattttga gacacaacgt gagatcaaag gatcttcttg agatcctttt
     1081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1201 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1441 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc gatcccgcga
     1681 aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt
     1741 aactttaaga aggagatata catatggaga aaaaaatctg gagccatccg cagttcgaaa
     1801 aaggcggatc cggaggcagc cacatgaccc gtacccgtat ggaaaatgaa ctgattgtga
     1861 gcaaaaacat gcagaacatt atcattgcag gtaatggtcc gagcctgaaa aacattaact
     1921 ataaacgtct gcctcgcgag tatgatgttt ttcgttgtaa ccagttctat ttcgaggata
     1981 aatactatct gggcaaaaaa atcaaagccg tgtttttcaa tccgggtgtt tttctgcagc
     2041 agtatcatac cgcaaaacag ctgattctga aaaacgagta cgagatcaaa aacatctttt
     2101 gcagcacctt taacctgccg tttattgaaa gcaacgattt cctgcaccag ttttacaact
     2161 tttttccgga tgcaaaactg ggctatgaag tgattgaaaa cctgaaagag ttctacgcct
     2221 atatcaaata caacgagatc tatttcaaca aacgcattac cagcggtgtt tatatgtgtg
     2281 caattgccat tgccctgggc tataaaacca tttatctgtg cggtatcgat ttctatgaag
     2341 gcgacgttat ttatccgttt gaagcaatga gcaccaacat taaaacaatc ttccctggca
     2401 tcaaagactt caaaccgagc aattgtcaca gcaaagaata tgatattgag gccctgaaac
     2461 tgctgaaaag catctataaa gtgaacatct atgccctgtg tgatgatagc attctggcaa
     2521 atcattttcc gctgagcatt aacatcaaca acaactttac cctggaaaac aaacacaaca
     2581 acagcatcaa tgatatcctg ctgaccgata atacaccggg tgttagcttt tacaaaaatc
     2641 agctgaaagc cgataacaaa attatgctga acttttatta a
//
