LOCUS       Exported                3050 bp DNA     circular SYN 24-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ariel
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Feb 24, 2023 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      66..113
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(131..946)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1068..1656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1684..1702
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1734..1756
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    1764..3017
                     /label=ConNeuA
     misc_feature    1764..3017
                     /label=CMP Sialic Acid Synthase
     CDS             3024..3047
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 gtcgaccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
       61 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      121 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
      181 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
      241 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
      301 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
      361 gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt
      421 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
      481 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
      541 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
      601 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
      661 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
      721 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
      781 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
      841 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
      901 ttaatcgcgg cttcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
      961 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     1021 tgtaacatca gagattttga gacacaacgt gagatcaaag gatcttcttg agatcctttt
     1081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1201 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1441 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc gatcccgcga
     1681 aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt
     1741 aactttaaga aggagatata catatgcgca ccaaaattat cgcaattatt ccggcacgta
     1801 gcggtagcaa aggtctgcgt aacaaaaatg cactgatgct gattgataaa ccgctgctgg
     1861 catataccat tgaagcagca ctgcagagcg aaatgtttga aaaagttatt gttaccaccg
     1921 acagcgaaca gtatggtgca attgccgaaa gctatggtgc agattttctg ctgcgtccgg
     1981 aagaactggc aaccgataaa gcaagcagct ttgaatttat caaacatgcc ctgagcatct
     2041 ataccgatta tgaaaatttt gcactgctgc agccgaccag tccgtttcgt gatagcaccc
     2101 atattattga agccgtgaaa ctgtatcaga ccctggaaaa gtatcagtgt gttgttagcg
     2161 ttacccgtag caataaaccg agccagatta ttcgtccgct ggatgattat agcaccctga
     2221 gcttttttga tctggactac agcaaatata accgcaacag cattgttgaa tatcatccga
     2281 atggtgccat ttttatcgcc aacaaacagc attatctgca taccaaacat tttttcggtc
     2341 gttatagcct ggcctacatc atggataaag aaagctcact ggatatcgat gatcgcatgg
     2401 attttgaact ggccattacc attcagcaga agaaaaatcg tcagaagatt ctgtaccaga
     2461 acatccataa tcgcatcaac gaaaaacgca acgaatttga tagcgtgagc gatattaccc
     2521 tgattggtca tagcctgttt gattattggg acgtgaaaaa gatcaacgac atcgaagtta
     2581 ataacctggg tattgccggt atcaacagca aagaatatta cgagtacatc atcgagaaag
     2641 agcgcattgt taattttggc gaattcgtgt ttatcttttt cggcaccaat gatattgttg
     2701 tgagcgactg gaaaaaagag gatacccttt ggtatctgaa aaagacctgc cagtacatca
     2761 aaaaaaagaa tgcagccagc aaaatctacc tgctgagcgt tccgcctgtt tttggtcgta
     2821 ttgatcgtga taatcgtatc atcaatgacc tgaatagcta tctgcgtgaa aatgtggatt
     2881 tcgccaaatt tatcagcctg gatcatgttc tgaaagattc ctatggcaac ctgaacaaaa
     2941 tgtataccta tgatggcctg cactttaaca gcaatggtta taccgttctg gaaaacgaaa
     3001 ttgccgagat cgttaaatct gcgtggagcc atccgcagtt cgaaaaataa
//
