LOCUS       SMC1998._pBMN_HA        7216 bp DNA     circular SYN 01-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   eliasa55
  TITLE     Direct Submission
  JOURNAL   Exported Feb 28, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             166..635
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    700..1057
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1122..1538
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1548..1922
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             1931..1960
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="MYPYDVPDYA"
     CDS             1991..4177
                     /codon_start=1
                     /label=TBK1
                     /translation="QSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFL
                     RPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAY
                     GLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAAREL
                     EDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRP
                     FEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLA
                     NILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYK
                     QTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLP
                     KVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVH
                     KKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETS
                     LQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAY
                     NEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQ
                     CFDIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKE
                     KMEGVVKELAENNHILERFGSLTMDGGLRNVDCL"
     primer_bind     4050..4095
                     /label=E696K_Fw
     primer_bind     complement(4050..4095)
                     /label=E696K
     misc_feature    4073..4075
                     /label=E696K
     LTR             4248..4841
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(5143..5731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5905..6765)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 taattactag tcaattagtt gatttttatt tttgacatat acatgtgaaa gaccccacct
       61 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
      121 tgagaataga aaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
      181 agcggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
      241 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      301 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
      361 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      421 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      481 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
      541 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
      601 tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga
      661 gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag caacttatct
      721 gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc ggtactagtt
      781 agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg gaacacccgg
      841 ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc cgacctgagt
      901 ccaaaaatcc cgatcgtttt ggactctttg gtgcaccccc cttagaggag ggatatgtgg
      961 ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg
     1021 tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg tgttgtctct
     1081 gtctgactgt gtttctgtat ttgtctgaaa atatgggccc gggccagact gttaccactc
     1141 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
     1201 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
     1261 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
     1321 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
     1381 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
     1441 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
     1501 cctcccttta tccagccctc actccttctc taggcgcccc catatggcca tatgagatct
     1561 tatatggggc acccccgccc cttgtaaact tccctgaccc tgacatgaca agagttacta
     1621 acagcccctc tctccaagct cacttacagg ctctctactt agtccagcac gaagtctgga
     1681 gacctctggc ggcagcctac caagaacaac tggaccgacc ggtggtacct cacccttacc
     1741 gagtcggcga cacagtgtgg gtccgccgac accagactaa gaacctagaa cctcgctgga
     1801 aaggacctta cacagtcctg ctgaccaccc ccaccgccct caaagtagac ggcatcgcag
     1861 cttggataca cgccgcccac gtgaaggctg ccgaccccgg gggtggacca tcctctagac
     1921 tgccgccacc atgtacccat acgatgttcc agattacgct tccggactca gatctcgagc
     1981 tcaagcttct cagagcactt ctaatcatct gtggctttta tctgatattt taggccaagg
     2041 agctactgca aatgtctttc gtggaagaca taagaaaact ggtgatttat ttgctatcaa
     2101 agtatttaat aacataagct tccttcgtcc agtggatgtt caaatgagag aatttgaagt
     2161 gttgaaaaaa ctcaatcaca aaaatattgt caaattattt gctattgaag aggagacaac
     2221 aacaagacat aaagtactta ttatggaatt ttgtccatgt gggagtttat acactgtttt
     2281 agaagaacct tctaatgcct atggactacc agaatctgaa ttcttaattg ttttgcgaga
     2341 tgtggtgggt ggaatgaatc atctacgaga gaatggtata gtgcaccgtg atatcaagcc
     2401 aggaaatatc atgcgtgtta taggggaaga tggacagtct gtgtacaaac tcacagattt
     2461 tggtgcagct agagaattag aagatgatga gcagtttgtt tctctgtatg gcacagaaga
     2521 atatttgcac cctgatatgt atgagagagc agtgctaaga aaagatcatc agaagaaata
     2581 tggagcaaca gttgatcttt ggagcattgg ggtaacattt taccatgcag ctactggatc
     2641 actgccattt agaccctttg aagggcctcg taggaataaa gaagtgatgt ataaaataat
     2701 tacaggaaag ccttctggtg caatatctgg agtacagaaa gcagaaaatg gaccaattga
     2761 ctggagtgga gacatgcctg tttcttgcag tctttctcgg ggtcttcagg ttctacttac
     2821 ccctgttctt gcaaacatcc ttgaagcaga tcaggaaaag tgttggggtt ttgaccagtt
     2881 ttttgcagaa actagtgata tacttcaccg aatggtaatt catgtttttt cgctacaaca
     2941 aatgacagct cataagattt atatacatag ctataatact gctactatat ttcatgaact
     3001 ggtatataaa caaaccaaaa ttatttcttc aaatcaagaa cttatctacg aagggcgacg
     3061 cttagtctta gaacctggaa ggctggcaca acatttccct aaaactactg aggaaaaccc
     3121 tatatttgta gtaagccggg aacctctgaa taccatagga ttaatatatg aaaaaatttc
     3181 cctccctaaa gtacatccac gttatgattt agacggggat gctagcatgg ctaaggcaat
     3241 aacaggggtt gtgtgttatg cctgcagaat tgccagtacc ttactgcttt atcaggaatt
     3301 aatgcgaaag gggatacgat ggctgattga attaattaaa gatgattaca atgaaactgt
     3361 tcacaaaaag acagaagttg tgatcacatt ggatttctgt atcagaaaca ttgaaaaaac
     3421 tgtgaaagta tatgaaaagt tgatgaagat caacctggaa gcggcagagt taggtgaaat
     3481 ttcagacata cacaccaaat tgttgagact ttccagttct cagggaacaa tagaaaccag
     3541 tcttcaggat atcgacagca gattatctcc aggtggatca ctggcagacg catgggcaca
     3601 tcaagaaggc actcatccga aagacagaaa tgtagaaaaa ctacaagtcc tgttaaattg
     3661 catgacagag atttactatc agttcaaaaa agacaaagca gaacgtagat tagcttataa
     3721 tgaagaacaa atccacaaat ttgataagca aaaactgtat taccatgcca caaaagctat
     3781 gacgcacttt acagatgaat gtgttaaaaa gtatgaggca tttttgaata agtcagaaga
     3841 atggataaga aagatgcttc atcttaggaa acagttatta tcgctgacta atcagtgttt
     3901 tgatattgaa gaagaagtat caaaatatca agaatatact aatgagttac aagaaactct
     3961 gcctcagaaa atgtttacag cttccagtgg aatcaaacat accatgaccc caatttatcc
     4021 aagttctaac acattagtag aaatgactct tggtatgaag aaattaaagg aaaagatgga
     4081 aggggtggtt aaagaacttg ctgaaaataa ccacatttta gaaaggtttg gctctttaac
     4141 catggatggt ggccttcgca acgttgactg tctttaggat ccaccggatc tagataactg
     4201 atcacgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     4261 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
     4321 taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
     4381 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
     4441 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
     4501 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
     4561 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
     4621 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
     4681 caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
     4741 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
     4801 cctctgagtg attgactacc cgtcagcggg ggtctttcat tcacatgcag catgtatcaa
     4861 aattaatttg gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa
     4921 tcggccaacg cgcggggaga ggcggtttgc gtattggcgc tcttccgctt cctcgctcac
     4981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5581 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5761 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5821 gatcttcacc tagatccttt taaattaaaa atgaagtttg cgcaaatcaa tctaaagtat
     5881 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5941 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6001 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6061 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6121 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6181 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6241 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6301 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6361 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6421 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6481 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     6541 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6601 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6661 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6721 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6781 atattattga agcatttatc agggttattg tctcatgaca ttaacctata aaaataggcg
     6841 tatcacgagg ccctttcgtc ttcaagaaca gctttgctct taggagtttc ctaatacatc
     6901 ccaaactcaa atatataaag catttgactt gttctatgcc ctagggggcg gggggaagct
     6961 aagccagctt tttttaacat ttaaaatgtt aattccattt taaatgcaca gatgttttta
     7021 tttcataagg gtttcaatgt gcatgaatgc tgcaatattc ctgttaccaa agctagtata
     7081 aataaaaata gataaacgtg gaaattactt agagtttctg tcattaacgt ttccttcctc
     7141 agttgacaac ataaatgcgc tgctgagaag ccagtttgca tctgtcagga tcaatttccc
     7201 attatgccag tcatat
//
