LOCUS       Exported                9690 bp ds-DNA     circular SYN 19-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pUt-mPF-BACH1 Cas9-Resistant_AmpR
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Yiming Wan
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 1, 2023 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     CDS             1..10
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="PGP"
     CDS             11..727
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             11..29
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGE"
     misc_feature    728..751
                     /note="yEGFP::ZeoR Linker"
     misc_feature    752..2959
                     /note="BACH1 with silent mutation"
     misc_feature    928
                     /note="silent mutation site"
     misc_feature    929..930
                     /note="BACH1-KO PAM"
     misc_feature    2993..3351
                     /note="WPRE"
     polyA_signal    3409..3633
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     polyA_site      3768..4162
                     /note="beta-globin poly(A)"
                     /note="human beta-globin polyadenylation signal"
     gene            complement(4170..4593)
                     /product="Blasticidin Resistance gene"
                     /note="BsrS2"
     promoter        complement(4602..4899)
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    4926..4944
                     /label=SA
                     /note="SA"
     misc_feature    complement(4945..4978)
                     /note="F3"
     intron          4979..5022
                     /label=HA1-AAVS1
                     /note="HA1-AAVS1"
     misc_feature    4985..5022
                     /note="HA-L"
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        5045..5149
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             5150..6010
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        6043..6539
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     5'UTR           6581..6620
                     /note="HSV TK 5'-UTR"
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             6640..7770
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /note="HSV TK"
                     /translation="MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQKATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGAAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    7812..7860
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7884..8472
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8477..8512)
                     /note="FRT (minimal)"
     promoter        8530
                     /note="CMV-D2i Promoter"
     promoter        8572..8886
                     /note="tight TRE promoter"
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    8580..8598
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8616..8634
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8651..8669
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8687..8705
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8723..8741
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8758..8776
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8794..8812
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8830..8885
                     /note="PminCMV?"
     TATA_signal     8848..8854
     TATA_signal     8877..8883
                     /note="Initiator Element"
     CDS             8891..9634
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /note="rtTA-Advanced"
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             9644..10
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ccctggacct atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
       61 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      121 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      181 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
      241 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      301 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      361 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      421 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
      481 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
      541 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      601 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
      661 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
      721 gtacaagggt gacggtgctg gtttaattaa catgtctctg agtgagaact cggtttttgc
      781 ctatgaatct tctgtgcata gcaccaatgt tttactcagc cttaatgacc agcggaagaa
      841 agatgtgctg tgcgatgtca ccatctttgt ggagggacag cggttccgcg ctcaccggtc
      901 cgtgctggcg gcatgcagca gttactttca ctcaagaatc gtaggccagg ctgatggaga
      961 gctgaacatt actcttccag aagaggtgac agttaaagga tttgaacctt taattcagtt
     1021 tgcctacact gctaaactga ttttaagtaa agagaatgtg gatgaagtgt gcaaatgtgt
     1081 ggagttttta agtgtacata atattgagga atcctgcttt cagtttctga aatttaagtt
     1141 tttggactcc actgcagacc agcaagaatg cccaagaaaa aaatgctttt catcacactg
     1201 tcagaaaaca gaccttaaac tttcactttt ggaccagagg gatctagaaa ctgatgaagt
     1261 ggaggaattt ctggaaaata aaaatgttca gactcctcag tgtaaactcc gcaggtatca
     1321 aggaaatgca aaagcctcac ctcctctaca agacagtgcc agtcagacat atgagtccat
     1381 gtgcttagag aaggatgctg ctctggcctt gccttcttta tgccccaaat acagaaaatt
     1441 ccaaaaagca tttggaactg acagagtccg tactggggaa tctagtgtca aagacattca
     1501 tgcttctgtt cagccaaatg aaaggtctga aaatgaatgc ctgggaggag tcccggagtg
     1561 tagagatttg caggtgatgt taaaatgtga cgaaagtaaa ttagcaatgg aacctgaaga
     1621 aacgaagaaa gatcctgctt ctcagtgccc aactgaaaaa tcagaagtga ctcctttccc
     1681 ccacaattct tccatagacc ctcatggact ttattctttg tctcttttac acacatatga
     1741 ccaatatggt gacttgaatt ttgctggtat gcaaaacaca acagtgttaa cagaaaagcc
     1801 tttgtcaggt acagacgtcc aagaaaaaac atttggtgaa agtcaggatt tacctttgaa
     1861 atccgacttg ggcaccaggg aagatagtag tgttgcatct agtgatagga gtagtgtgga
     1921 gcgagaagtg gcagaacacc tagcaaaagg cttctggagt gacatttgca gcacggacac
     1981 tccttgccaa atgcagttat cacctgctgt ggccaaagat ggctcagaac agatctcaca
     2041 gaaacggtct gagtgtccgt ggttaggtat caggattagt gagagcccag aaccaggtca
     2101 aaggactttc acaacattaa gttctgtcaa ctgccctttt ataagtactc tgagtactga
     2161 aggctgttca agcaatttgg aaattggaaa cgatgattat gtttcagaac cccagcaaga
     2221 accttgccca tatgcttgtg tcattagctt gggagacgac tctgagacgg acaccgaagg
     2281 agacagtgaa tcctgttcag ccagagaaca agaatgtgag gtaaaactgc cattcaatgc
     2341 acaacggata atttcactgt ctcgaaatga ttttcagtcc ttgttgaaaa tgcacaagct
     2401 tactccagaa cagctggatt gtatccatga tattcgaaga agaagtaaaa acagaattgc
     2461 tgcacagcgc tgtcgcaaga gaaaacttga ctgtatacag aatcttgaat cagaaattga
     2521 gaagctgcaa agtgaaaagg agagcttgtt gaaggaaaga gatcacattt tgtcaactct
     2581 gggtgagaca aagcagaacc taactggact ttgccagaaa gtttgtaaag aagcagctct
     2641 gagtcaagaa caaatacaga tactcgccaa gtactcagct gcagattgcc cactttcatt
     2701 tttaatttct gaaaaagata aaagtactcc tgatggtgaa ctggcgttac catcaatttt
     2761 cagtttatct gaccggcctc cagcagtgct gcctccctgt gccagaggaa acagtgagcc
     2821 tggctacgcg cgagggcagg agtcccagca gatgtccaca gccacctctg agcaagctgg
     2881 gcctgcggaa cagtgtcgtc agagtggtgg gatctcagat ttctgtcagc agatgactga
     2941 taaatgtact actgatgagt aaaccggtgc ggccgcaatc aacctctgga ttacaaaatt
     3001 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3061 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3121 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3181 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3241 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3361 ttgtcctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     3421 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3481 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3541 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     3601 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     3661 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3721 ttacgcgcta aagcaagtaa aacctctaca aatgtggtat ggaattctaa aatacagcat
     3781 agcaaaactt taacctccaa atcaagcctc tacttgaatc cttttctgag ggatgaataa
     3841 ggcataggca tcaggggctg ttgccaatgt gcattagctg tttgcagcct caccttcttt
     3901 catggagttt aagatatagt gtattttccc aaggtttgaa ctagctcttc atttctttat
     3961 gttttaaatg cactgacctc ccacattccc tttttagtaa aatattcaga aataatttaa
     4021 atacatcatt gcaatgaaaa taaatgtttt ttattaggca gaatccagat gctcaaggcc
     4081 cttcataata tcccccagtt tagtagttgg acttagggaa caaaggaacc tttaatagaa
     4141 attggacagc aagaaagcga gcttctagct ttagttcctg gtgtacttga gggggatgag
     4201 ttcctcaatg gtggttttga ccagcttgcc attcatctca atgagcacaa agcagtcagg
     4261 agcatagtca gagatgagct ctctgcacat gccacagggg ctgaccaccc tgatggatct
     4321 gtccacctca tcagagtagg ggtgcctgac agccacaatg gtgtcaaagt ccttctgccc
     4381 gttgctcaca gcagacccaa tggcaatggc ttcagcacag acagtgaccc tgccaatgta
     4441 ggcctcaatg tggacagcag agatgatctc cccagtcttg gtcctgatgg ccgccccgac
     4501 atggtgcttg ttgtcctcat agagcatggt gatcttctca gtggcgacct ccaccagctc
     4561 cagatcctgc tgagagatgt tgaaggtctt catggtagcc tccaaaaaag cctcctcact
     4621 acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta
     4681 gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg atgggcggag
     4741 ttaggggcgg gactatggtt gctgactaat tgagatgcat gctttgcata cttctgcctg
     4801 ctggggagcc tggggacttt ccacacctgg ttgctgacta attgagatgc atgctttgca
     4861 tacttctgcc tgctggggag cctggggact ttccacaccc taactgacac acattccaca
     4921 gctgcgaaga gaagaggtca ggccgaagtt cctatactat ttgaagaata ggaacttcaa
     4981 gcttgcccca ctgtggggtg gaggggacag ataaaagtac cctggcactt ttcggggaaa
     5041 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5101 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5161 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5221 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5281 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5341 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5401 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5461 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5521 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5581 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5641 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5701 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5761 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5821 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5881 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5941 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6001 gcattggtaa ctgtcagacc aagtttattc tacggttcta ccgggtaggg gaggcgcttt
     6061 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     6121 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     6181 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     6241 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     6301 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     6361 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     6421 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     6481 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     6541 cgacctgcag cgacccgcta acagcgtcaa cagcgtgccg cagatcttgg tggcgtgaaa
     6601 ctcccgcacc tcttcggcca gcgccttgta gaagcgcgta tggcttcgta cccctgccat
     6661 caacacgcgt ctgcgttcga ccaggctgcg cgttctcgcg gccataacaa ccgacgtacg
     6721 gcgttgcgcc ctcgccggca acaaaaagcc acggaagtcc gcctggagca gaaaatgccc
     6781 acgctactgc gggtttatat agacggtccc cacgggatgg ggaaaaccac caccacgcaa
     6841 ctgctggtgg ccctgggttc gcgcgacgat atcgtctacg tacccgagcc gatgacttac
     6901 tggcgggtgt tgggggcttc cgagacaatc gcgaacatct acaccacaca acaccgcctc
     6961 gaccagggtg agatatcggc cggggacgcg gcggtggtaa tgacaagcgc ccagataaca
     7021 atgggcatgc cttatgccgt gaccgacgcc gttctggctc ctcatatcgg gggggaggct
     7081 gggagctcac atgccccgcc cccggccctc accctcatct tcgaccgcca tcccatcgcc
     7141 gccctcctgt gctacccggc cgcgcgatac cttatgggca gcatgacccc ccaggccgtg
     7201 ctggcgttcg tggccctcat cccgccgacc ttgcccggca caaacatcgt gttgggggcc
     7261 cttccggagg acagacacat cgaccgcctg gccaaacgcc agcgccccgg cgagcggctt
     7321 gacctggcta tgctggccgc gattcgccgc gtttatgggc tgcttgccaa tacggtgcgg
     7381 tatctgcagg gcggcgggtc gtggcgggag gattggggac agctttcggg ggcggccgtg
     7441 ccgccccagg gtgccgagcc ccagagcaac gcgggcccac gaccccatat cggggacacg
     7501 ttatttaccc tgtttcgggc ccccgagttg ctggccccca acggcgacct gtacaacgtg
     7561 tttgcctggg ccttggacgt cttggccaaa cgcctccgtc ccatgcacgt ctttatcctg
     7621 gattacgacc aatcgcccgc cggctgccgg gacgccctgc tgcaacttac ctccgggatg
     7681 gtccagaccc acgtcaccac ccccggctcc ataccgacga tctgcgacct ggcgcgcacg
     7741 tttgcccggg agatggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     7801 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
     7861 ataaacgcgc tcataggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7921 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7981 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8041 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8101 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8161 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8221 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8281 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8341 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8401 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8461 agcctatgga aaaacgccga agttcctata ctttctagag aataggaact tcacgggcca
     8521 gatatacgcg acgggccaga tatacgcgtc gaggcccttt cgtcttcact cgagtttact
     8581 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg
     8641 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
     8701 agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc
     8761 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     8821 cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     8881 atcgcctgga atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact
     8941 caatggagtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     9001 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     9061 cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga
     9121 ctttctgcgg aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc
     9181 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     9241 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     9301 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     9361 aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt
     9421 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     9481 ggagaaacag ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt
     9541 agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc
     9601 tcttgacgat tttgaccttg acatgctccc cgggggaagc ggagctacta acttcagcct
     9661 gctgaagcag gctggagacg tggaggagaa
//
