LOCUS       Exported                2290 bp DNA     circular SYN 28-FEB-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Qian
  TITLE     Direct Submission
  JOURNAL   Exported Feb 28, 2023 from SnapGene 6.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2290
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        82..322
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    331..353
                     /label=SagRNA.CLYBL
                     /note="color: #ff0000"
     misc_feature    354..437
                     /label=SagRNA scaffold
                     /note="color: #00ccff; direction: RIGHT"
     misc_feature    438..444
                     /label=termination signal
                     /note="color: #ff0000"
     rep_origin      complement(541..1129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     gene            complement(1300..2160)
                     /gene="Ampicillin"
                     /label=Ampicillin
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(1300..2160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1300 .. 2091 / #ccffcc
                        2: 2092 .. 2160 / #ccffcc / signal sequence
                     Cleavage site after base 2091"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2161..2265)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc gcctacatac cgttatcctg tgggttttag
      361 tactctgtaa tgaaaattac agaatctact aaaacaaggc aaaatgccgt gtttatctcg
      421 tcaacttgtt ggcgagattt ttttaagctt gggccgctcg aggtacctct ctacatatga
      481 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      541 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      601 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      661 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      721 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      781 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      841 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      901 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      961 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1021 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1081 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1141 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1201 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1261 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1321 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1381 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1441 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1501 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1561 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1621 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1681 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1741 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1801 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1861 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1921 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1981 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2041 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2101 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2161 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2221 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2281 agtgccacct
//
