LOCUS       Exported                9360 bp DNA     circular SYN 01-MAR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Kim Janssen
  TITLE     Direct Submission
  JOURNAL   Exported Mar 1, 2023 from SnapGene 6.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9360
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    31..1752
                     /label=CAG
                     /note="color: #a6acb3; direction: RIGHT"
     enhancer        33..412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        415..690
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          691..1699
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     regulatory      1838..1847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             1847..1912
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 1912"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1919..1939
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             1964..6064
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=SpCas9-D10A-K848A-R1060A
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain
                     
                     carries the mutations K848A and R1060A"
                     /note="This feature has 5 segments:
                        1: 1964 .. 4501 / #993366
                        2: 4502 .. 4504 / #cc99b2 / K848A
                        3: 4505 .. 5137 / #993366
                        4: 5138 .. 5140 / #cc99b2 / R1060A
                        5: 5141 .. 6064 / #993366"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    1988..1990
                     /label=D10A
                     /note="color: #ff0000"
     misc_feature    4502..4504
                     /label=K848A
                     /note="color: #02abea"
     misc_feature    4967..4969
                     /label=K1003
                     /note="color: #ff0000"
     misc_feature    5138..5140
                     /label=R1060A
                     /note="color: #02abea"
     CDS             6065..6112
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /note="color: #993366"
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    6147..6673
                     /label=rBGpA
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_site      6294..6349
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6726..6742)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     promoter        7216..7320
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7321..8181
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7321 .. 7389 / #ccffcc / signal sequence
                        2: 7390 .. 8181 / #ccffcc
                     Cleavage site after base 7389"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGARERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8352..8940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     promoter        9264..9294
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9264 .. 9269 / #ffffff / -35
                        2: 9270 .. 9287 / #ffffff
                        3: 9288 .. 9294 / #ffffff / -10"
     primer_bind     9326..9342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 gtttaaacat ttaaatctcg agccatggat tcgacattga ttattgacta gttattaata
       61 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      121 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      181 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      241 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      301 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      361 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tgggtcgagg
      421 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
      481 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg
      541 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca
      601 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
      661 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc gccccgtgcc
      721 ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
      781 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
      841 ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg ccctttgtgc
      901 gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
      961 cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     1021 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg gctgcgaggg
     1081 gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcggc
     1141 ggtcgggctg taaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     1201 cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc
     1261 ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg
     1321 ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct
     1381 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct ggcggagccg
     1441 aaatctggga ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg tgcggcgccg
     1501 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
     1561 atctccagcc tcggggctgc cgcaggggga cggctgcctt cgggggggac ggggcagggc
     1621 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
     1681 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt ctcatcattt
     1741 tggcaaagaa ttatcgcatg cctgcagagc tctagagtct aatgtttaat tacctggagc
     1801 acctgcctga aatcactttt tttcaggttg gaccggtgcc accatggact ataaggacca
     1861 cgacggagac tacaaggatc atgatattga ttacaaagac gatgacgata agatggcccc
     1921 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat
     1981 cggcctggct atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
     2041 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
     2101 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
     2161 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
     2221 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
     2281 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
     2341 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
     2401 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
     2461 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
     2521 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
     2581 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc
     2641 ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg
     2701 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
     2761 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
     2821 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
     2881 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
     2941 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
     3001 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc
     3061 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
     3121 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa
     3181 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
     3241 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
     3301 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
     3361 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc
     3421 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
     3481 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt
     3541 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc
     3601 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
     3661 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
     3721 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
     3781 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
     3841 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
     3901 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
     3961 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
     4021 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
     4081 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
     4141 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
     4201 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
     4261 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
     4321 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
     4381 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
     4441 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct
     4501 ggccgacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag
     4561 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct
     4621 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg
     4681 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
     4741 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga
     4801 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
     4861 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
     4921 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
     4981 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
     5041 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
     5101 gaccgagatt accctggcca acggcgagat ccggaaggcg cctctgatcg agacaaacgg
     5161 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct
     5221 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
     5281 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga
     5341 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
     5401 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
     5461 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
     5521 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
     5581 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
     5641 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
     5701 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga
     5761 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
     5821 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga
     5881 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
     5941 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
     6001 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
     6061 cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agtaagaatt
     6121 ggccgcactt aagttacgcg tggatcaatt cactcctcag gtgcaggctg cctatcagaa
     6181 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     6241 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     6301 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     6361 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     6421 atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga
     6481 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     6541 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     6601 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     6661 tcttatgaag atccctcgac ctgcagccca agctgatccc gggatttaaa tgtttaaacg
     6721 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     6781 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     6841 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     6901 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     6961 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     7021 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     7081 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     7141 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     7201 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7261 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7321 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     7381 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     7441 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     7501 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     7561 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     7621 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     7681 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     7741 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     7801 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     7861 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     7921 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     7981 tcggcccttc cggctggctg gtttattgct gataaatctg gagcccgtga gcgtgggtct
     8041 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8101 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8161 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8221 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8281 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     8341 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     8401 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     8461 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     8521 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     8581 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     8641 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     8701 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     8761 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     8821 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     8881 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     8941 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9001 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9061 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     9181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
//
