LOCUS       Exported                9450 bp DNA     circular SYN 01-MAR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 1, 2023 from SnapGene 6.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        212..452
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    461..480
                     /label=AAVS1-T2 gRNA spacer
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        481..556
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
     misc_feature    558..563
                     /label=termination signal
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    756..2477
                     /label=CAG
                     /note="color: #a6acb3; direction: RIGHT"
     enhancer        758..1137
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        1140..1415
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          1416..2424
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     regulatory      2523..2532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             2529..6632
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6645..6665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    6707..6977
                     /label=TKpA
                     /note="color: #a6acb3; direction: RIGHT"
     polyA_signal    6764..6812
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     promoter        7416..7520
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7521..8381
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7521 .. 7589 / #ccffcc / signal sequence
                        2: 7590 .. 8381 / #ccffcc
                     Cleavage site after base 7589"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8552..9140
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     9378..9394
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 atgttattcc tccttattta aaagcttaag gtgcacggcc cacgtggcca ctagtacttc
       61 tcgagctctg tacatgtccg cggtcgcgac gtacgcgtat cgatggcgcc agctgcaggc
      121 ggccgccata tgcatcctag gcctattaat attccggagt atacgtagcc ggctaacgtt
      181 gactggatcc ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata
      241 tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac
      301 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
      361 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
      421 tttcttggct ttatatatct tgtggaaagg acgaaacacc ggggccacta gggacaggat
      481 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      541 ggcaccgagt cggtgctttt tttctagacc cagctttctt gtacaacaac cggtacctct
      601 agaactatag ctagcatgcg caaatttaaa gcgctgatat cgatcgcgcg cagatctgtc
      661 atgatgatca ttgcaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      721 gcgatgtacg ggccagatat actcgagcca tggattcgac attgattatt gactagttat
      781 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      841 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      901 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      961 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     1021 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     1081 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgggt
     1141 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     1201 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc
     1261 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     1321 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     1381 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgttg ccttcgcccc
     1441 gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     1501 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     1561 gacggctcgt ttcttttctg tggctgcgtg aaagccttaa agggctccgg gagggccctt
     1621 tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     1681 tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     1741 tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg ggggggctgc
     1801 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     1861 gcggcggtcg ggctgtaacc cccccctgca cccccctccc cgagttgctg agcacggccc
     1921 ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg ctcgccgtgc cgggcggggg
     1981 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
     2041 ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
     2101 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctggcgg
     2161 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcgggcga agcggtgcgg
     2221 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     2281 tctccatctc cagcctcggg gctgccgcag ggggacggct gccttcgggg gggacggggc
     2341 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     2401 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttgttgt gctgtctcat
     2461 cattttggca aagaattatc gcatgcctgc agagctctag agtcctagag gatcgaaccc
     2521 ttgccaccat ggacaagaag tactccattg ggctcgatat cggcacaaac agcgtcggct
     2581 gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata
     2641 ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac tccggggaga
     2701 cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc agaaagaatc
     2761 ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat gactctttct
     2821 tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag cgccacccaa
     2881 tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc atatatcatc
     2941 tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc tatctcgcgc
     3001 tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg aacccagaca
     3061 acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag cttttcgaag
     3121 agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct aggctgtcca
     3181 aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag aacggcctgt
     3241 ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct aacttcgacc
     3301 tggccgaaga tgccaagctt caactgagca aagacaccta cgatgatgat ctcgacaatc
     3361 tgctggccca gatcggcgac cagtacgcag accttttttt ggcggcaaag aacctgtcag
     3421 acgccattct gctgagtgat attctgcgag tgaacacgga gatcaccaaa gctccgctga
     3481 gcgctagtat gatcaagcgc tatgatgagc accaccaaga cttgactttg ctgaaggccc
     3541 ttgtcagaca gcaactgcct gagaagtaca aggaaatttt cttcgatcag tctaaaaatg
     3601 gctacgccgg atacattgac ggcggagcaa gccaggagga attttacaaa tttattaagc
     3661 ccatcttgga aaaaatggac ggcaccgagg agctgctggt aaagcttaac agagaagatc
     3721 tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc ccaccagatt cacctgggcg
     3781 aactgcacgc tatcctcagg cggcaagagg atttctaccc ctttttgaaa gataacaggg
     3841 aaaagattga gaaaatcctc acatttcgga taccctacta tgtaggcccc ctcgcccggg
     3901 gaaattccag attcgcgtgg atgactcgca aatcagaaga gaccatcact ccctggaact
     3961 tcgaggaagt cgtggataag ggggcctctg cccagtcctt catcgaaagg atgactaact
     4021 ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca ctctctgctg tacgagtact
     4081 tcacagttta taacgagctc accaaggtca aatacgtcac agaagggatg agaaagccag
     4141 cattcctgtc tggagagcag aagaaagcta tcgtggacct cctcttcaag acgaaccgga
     4201 aagttaccgt gaaacagctc aaagaagact atttcaaaaa gattgaatgt ttcgactctg
     4261 ttgaaatcag cggagtggag gatcgcttca acgcatccct gggaacgtat cacgatctcc
     4321 tgaaaatcat taaagacaag gacttcctgg acaatgagga gaacgaggac attcttgagg
     4381 acattgtcct cacccttacg ttgtttgaag atagggagat gattgaagaa cgcttgaaaa
     4441 cttacgctca tctcttcgac gacaaagtca tgaaacagct caagaggcgc cgatatacag
     4501 gatgggggcg gctgtcaaga aaactgatca atgggatccg agacaagcag agtggaaaga
     4561 caatcctgga ttttcttaag tccgatggat ttgccaaccg gaacttcatg cagttgatcc
     4621 atgatgactc tctcaccttt aaggaggaca tccagaaagc acaagtttct ggccaggggg
     4681 acagtcttca cgagcacatc gctaatcttg caggtagccc agctatcaaa aagggaatac
     4741 tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat gggaaggcat aagcccgaga
     4801 atatcgttat cgagatggcc cgagagaacc aaactaccca gaagggacag aagaacagta
     4861 gggaaaggat gaagaggatt gaagagggta taaaagaact ggggtcccaa atccttaagg
     4921 aacacccagt tgaaaacacc cagcttcaga atgagaagct ctacctgtac tacctgcaga
     4981 acggcaggga catgtacgtg gatcaggaac tggacatcaa tcggctctcc gactacgacg
     5041 tggatcatat cgtgccccag tcttttctca aagatgattc tattgataat aaagtgttga
     5101 caagatccga taaaaataga gggaagagtg ataacgtccc ctcagaagaa gttgtcaaga
     5161 aaatgaaaaa ttattggcgg cagctgctga acgccaaact gatcacacaa cggaagttcg
     5221 ataatctgac taaggctgaa cgaggtggcc tgtctgagtt ggataaagcc ggcttcatca
     5281 aaaggcagct tgttgagaca cgccagatca ccaagcacgt ggcccaaatt ctcgattcac
     5341 gcatgaacac caagtacgat gaaaatgaca aactgattcg agaggtgaaa gttattactc
     5401 tgaagtctaa gctggtctca gatttcagaa aggactttca gttttataag gtgagagaga
     5461 tcaacaatta ccaccatgcg catgatgcct acctgaatgc agtggtaggc actgcactta
     5521 tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg agactataaa gtgtacgatg
     5581 ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa ggccaccgct aagtacttct
     5641 tttacagcaa tattatgaat tttttcaaga ccgagattac actggccaat ggagagattc
     5701 ggaagcgacc acttatcgaa acaaacggag aaacaggaga aatcgtgtgg gacaagggta
     5761 gggatttcgc gacagtccgg aaggtcctgt ccatgccgca ggtgaacatc gttaaaaaga
     5821 ccgaagtaca gaccggaggc ttctccaagg aaagtatcct cccgaaaagg aacagcgaca
     5881 agctgatcgc acgcaaaaaa gattgggacc ccaagaaata cggcggattc gattctccta
     5941 cagtcgctta cagtgtactg gttgtggcca aagtggagaa agggaagtct aaaaaactca
     6001 aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg atcaagcttc gaaaaaaacc
     6061 ccatcgactt tctcgaggcg aaaggatata aagaggtcaa aaaagacctc atcattaagc
     6121 ttcccaagta ctctctcttt gagcttgaaa acggccggaa acgaatgctc gctagtgcgg
     6181 gcgagctgca gaaaggtaac gagctggcac tgccctctaa atacgttaat ttcttgtatc
     6241 tggccagcca ctatgaaaag ctcaaagggt ctcccgaaga taatgagcag aagcagctgt
     6301 tcgtggaaca acacaaacac taccttgatg agatcatcga gcaaataagc gaattctcca
     6361 aaagagtgat cctcgccgac gctaacctcg ataaggtgct ttctgcttac aataagcaca
     6421 gggataagcc catcagggag caggcagaaa acattatcca cttgtttact ctgaccaact
     6481 tgggcgcgcc tgcagccttc aagtacttcg acaccaccat agacagaaag cggtacacct
     6541 ctacaaagga ggtcctggac gccacactga ttcatcagtc aattacgggg ctctatgaaa
     6601 caagaatcga cctctctcag ctcggtggag acagcagggc tgaccccaag aagaagagga
     6661 aggtgtgaaa gggttcgatc cctaccggtt agtaatgagt ttaaacgggg gaggctaact
     6721 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     6781 aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     6841 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     6901 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     6961 ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc
     7021 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     7081 tgccagcgcc ctagcgcccg gggttataat tacctcaggt cgacgtccca tgtgcaggtg
     7141 ctgaattcga aggcattcca cgacgtggtc accagccatg gccatgtcca actccatcaa
     7201 atcagcttga gtagccatgc ccatggctcc agcctgtcct cgtaccagct ttgttccctt
     7261 tagtgagggg taatttcgag cttggcgtaa tcatggtcat agctgtttcc aattcttgaa
     7321 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     7381 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7441 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7501 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7561 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7621 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7681 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7741 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     7801 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7861 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7921 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7981 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     8041 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     8101 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8161 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8221 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8281 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8341 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8401 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8461 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8521 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8581 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8641 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     8701 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8761 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8821 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8881 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8941 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     9001 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9061 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9121 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9181 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9241 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9301 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     9361 gtatttcaca ccgcatagta aaacgacggc cagtgaattg taatacgact cactataggg
     9421 cgaattggag cttggcgtaa tcatggtcat
//
