LOCUS       Exported                9447 bp DNA     circular SYN 01-MAR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 1, 2023 from SnapGene 6.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        225..465
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    474..493
                     /label=Non-targeting (NT) gRNA spacer
                     /note="color: #a6acb3; direction: RIGHT"
     misc_RNA        494..569
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
                     /note="color: #00ccff; direction: RIGHT"
     misc_feature    753..2474
                     /label=CAG
                     /note="color: #a6acb3; direction: RIGHT"
     enhancer        755..1134
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        1137..1412
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     intron          1413..2421
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     regulatory      2520..2529
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             2526..6629
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /note="color: #993366"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6642..6662
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     polyA_signal    6761..6809
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     promoter        7413..7517
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7518..8378
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7518 .. 7586 / #ccffcc / signal sequence
                        2: 7587 .. 8378 / #ccffcc
                     Cleavage site after base 7586"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8549..9137
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     9375..9391
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 atgttattcc tccttattta aaagcttaag gtgcacggcc cacgtggcca ctagtacttc
       61 tcgagctctg tacatgtccg cggtcgcgac gtacgcgtat cgatggcgcc agctgcaggc
      121 ggccgccata tgcatcctag gcctattaat attccggagt atacgtagcc ggctaacgtt
      181 atcgcatgcc tgcagagctc tagagtccgg ccgccccctt caccgagggc ctatttccca
      241 tgattccttc atatttgcat atacgataca aggctgttag agagataatt ggaattaatt
      301 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
      361 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
      421 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accggtgagc
      481 tcttatttgc gtagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      541 aacttgaaaa agtggcaccg agtcggtgct ttttttgaat taacaaccgg tacctctaga
      601 actatagcta gcatgcgcaa atttaaagcg ctgatatcga tcgcgcgcag atctgtcatg
      661 atgatcattg caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
      721 atgtacgggc cagatatact cgagccatgg attcgacatt gattattgac tagttattaa
      781 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      841 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      901 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      961 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     1021 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     1081 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catgggtcga
     1141 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
     1201 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggcgcg
     1261 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
     1321 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
     1381 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg
     1441 ccccgctccg cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
     1501 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
     1561 ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt
     1621 gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
     1681 ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
     1741 gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg gggctgcgag
     1801 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     1861 gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     1921 ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     1981 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     2041 ggggcgcggc ggccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     2101 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctggcggagc
     2161 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc
     2221 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct
     2281 ccatctccag cctcggggct gccgcagggg gacggctgcc ttcggggggg acggggcagg
     2341 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     2401 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat
     2461 tttggcaaag aattatcgca tgcctgcaga gctctagagt cctagaggat cgaacccttg
     2521 ccaccatgga caagaagtac tccattgggc tcgatatcgg cacaaacagc gtcggctggg
     2581 ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt caaagttctg ggcaataccg
     2641 atcgccacag cataaagaag aacctcattg gcgccctcct gttcgactcc ggggagacgg
     2701 ccgaagccac gcggctcaaa agaacagcac ggcgcagata tacccgcaga aagaatcgga
     2761 tctgctacct gcaggagatc tttagtaatg agatggctaa ggtggatgac tctttcttcc
     2821 ataggctgga ggagtccttt ttggtggagg aggataaaaa gcacgagcgc cacccaatct
     2881 ttggcaatat cgtggacgag gtggcgtacc atgaaaagta cccaaccata tatcatctga
     2941 ggaagaagct tgtagacagt actgataagg ctgacttgcg gttgatctat ctcgcgctgg
     3001 cgcatatgat caaatttcgg ggacacttcc tcatcgaggg ggacctgaac ccagacaaca
     3061 gcgatgtcga caaactcttt atccaactgg ttcagactta caatcagctt ttcgaagaga
     3121 acccgatcaa cgcatccgga gttgacgcca aagcaatcct gagcgctagg ctgtccaaat
     3181 cccggcggct cgaaaacctc atcgcacagc tccctgggga gaagaagaac ggcctgtttg
     3241 gtaatcttat cgccctgtca ctcgggctga cccccaactt taaatctaac ttcgacctgg
     3301 ccgaagatgc caagcttcaa ctgagcaaag acacctacga tgatgatctc gacaatctgc
     3361 tggcccagat cggcgaccag tacgcagacc tttttttggc ggcaaagaac ctgtcagacg
     3421 ccattctgct gagtgatatt ctgcgagtga acacggagat caccaaagct ccgctgagcg
     3481 ctagtatgat caagcgctat gatgagcacc accaagactt gactttgctg aaggcccttg
     3541 tcagacagca actgcctgag aagtacaagg aaattttctt cgatcagtct aaaaatggct
     3601 acgccggata cattgacggc ggagcaagcc aggaggaatt ttacaaattt attaagccca
     3661 tcttggaaaa aatggacggc accgaggagc tgctggtaaa gcttaacaga gaagatctgt
     3721 tgcgcaaaca gcgcactttc gacaatggaa gcatccccca ccagattcac ctgggcgaac
     3781 tgcacgctat cctcaggcgg caagaggatt tctacccctt tttgaaagat aacagggaaa
     3841 agattgagaa aatcctcaca tttcggatac cctactatgt aggccccctc gcccggggaa
     3901 attccagatt cgcgtggatg actcgcaaat cagaagagac catcactccc tggaacttcg
     3961 aggaagtcgt ggataagggg gcctctgccc agtccttcat cgaaaggatg actaactttg
     4021 ataaaaatct gcctaacgaa aaggtgcttc ctaaacactc tctgctgtac gagtacttca
     4081 cagtttataa cgagctcacc aaggtcaaat acgtcacaga agggatgaga aagccagcat
     4141 tcctgtctgg agagcagaag aaagctatcg tggacctcct cttcaagacg aaccggaaag
     4201 ttaccgtgaa acagctcaaa gaagactatt tcaaaaagat tgaatgtttc gactctgttg
     4261 aaatcagcgg agtggaggat cgcttcaacg catccctggg aacgtatcac gatctcctga
     4321 aaatcattaa agacaaggac ttcctggaca atgaggagaa cgaggacatt cttgaggaca
     4381 ttgtcctcac ccttacgttg tttgaagata gggagatgat tgaagaacgc ttgaaaactt
     4441 acgctcatct cttcgacgac aaagtcatga aacagctcaa gaggcgccga tatacaggat
     4501 gggggcggct gtcaagaaaa ctgatcaatg ggatccgaga caagcagagt ggaaagacaa
     4561 tcctggattt tcttaagtcc gatggatttg ccaaccggaa cttcatgcag ttgatccatg
     4621 atgactctct cacctttaag gaggacatcc agaaagcaca agtttctggc cagggggaca
     4681 gtcttcacga gcacatcgct aatcttgcag gtagcccagc tatcaaaaag ggaatactgc
     4741 agaccgttaa ggtcgtggat gaactcgtca aagtaatggg aaggcataag cccgagaata
     4801 tcgttatcga gatggcccga gagaaccaaa ctacccagaa gggacagaag aacagtaggg
     4861 aaaggatgaa gaggattgaa gagggtataa aagaactggg gtcccaaatc cttaaggaac
     4921 acccagttga aaacacccag cttcagaatg agaagctcta cctgtactac ctgcagaacg
     4981 gcagggacat gtacgtggat caggaactgg acatcaatcg gctctccgac tacgacgtgg
     5041 atcatatcgt gccccagtct tttctcaaag atgattctat tgataataaa gtgttgacaa
     5101 gatccgataa aaatagaggg aagagtgata acgtcccctc agaagaagtt gtcaagaaaa
     5161 tgaaaaatta ttggcggcag ctgctgaacg ccaaactgat cacacaacgg aagttcgata
     5221 atctgactaa ggctgaacga ggtggcctgt ctgagttgga taaagccggc ttcatcaaaa
     5281 ggcagcttgt tgagacacgc cagatcacca agcacgtggc ccaaattctc gattcacgca
     5341 tgaacaccaa gtacgatgaa aatgacaaac tgattcgaga ggtgaaagtt attactctga
     5401 agtctaagct ggtctcagat ttcagaaagg actttcagtt ttataaggtg agagagatca
     5461 acaattacca ccatgcgcat gatgcctacc tgaatgcagt ggtaggcact gcacttatca
     5521 aaaaatatcc caagcttgaa tctgaatttg tttacggaga ctataaagtg tacgatgtta
     5581 ggaaaatgat cgcaaagtct gagcaggaaa taggcaaggc caccgctaag tacttctttt
     5641 acagcaatat tatgaatttt ttcaagaccg agattacact ggccaatgga gagattcgga
     5701 agcgaccact tatcgaaaca aacggagaaa caggagaaat cgtgtgggac aagggtaggg
     5761 atttcgcgac agtccggaag gtcctgtcca tgccgcaggt gaacatcgtt aaaaagaccg
     5821 aagtacagac cggaggcttc tccaaggaaa gtatcctccc gaaaaggaac agcgacaagc
     5881 tgatcgcacg caaaaaagat tgggacccca agaaatacgg cggattcgat tctcctacag
     5941 tcgcttacag tgtactggtt gtggccaaag tggagaaagg gaagtctaaa aaactcaaaa
     6001 gcgtcaagga actgctgggc atcacaatca tggagcgatc aagcttcgaa aaaaacccca
     6061 tcgactttct cgaggcgaaa ggatataaag aggtcaaaaa agacctcatc attaagcttc
     6121 ccaagtactc tctctttgag cttgaaaacg gccggaaacg aatgctcgct agtgcgggcg
     6181 agctgcagaa aggtaacgag ctggcactgc cctctaaata cgttaatttc ttgtatctgg
     6241 ccagccacta tgaaaagctc aaagggtctc ccgaagataa tgagcagaag cagctgttcg
     6301 tggaacaaca caaacactac cttgatgaga tcatcgagca aataagcgaa ttctccaaaa
     6361 gagtgatcct cgccgacgct aacctcgata aggtgctttc tgcttacaat aagcacaggg
     6421 ataagcccat cagggagcag gcagaaaaca ttatccactt gtttactctg accaacttgg
     6481 gcgcgcctgc agccttcaag tacttcgaca ccaccataga cagaaagcgg tacacctcta
     6541 caaaggaggt cctggacgcc acactgattc atcagtcaat tacggggctc tatgaaacaa
     6601 gaatcgacct ctctcagctc ggtggagaca gcagggctga ccccaagaag aagaggaagg
     6661 tgtgaaaggg ttcgatccct accggttagt aatgagttta aacgggggag gctaactgaa
     6721 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     6781 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     6841 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     6901 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     6961 aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg
     7021 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7081 cagcgcccta gcgcccgggg ttataattac ctcaggtcga cgtcccatgt gcaggtgctg
     7141 aattcgaagg cattccacga cgtggtcacc agccatggcc atgtccaact ccatcaaatc
     7201 agcttgagta gccatgccca tggctccagc ctgtcctcgt accagctttg ttccctttag
     7261 tgaggggtaa tttcgagctt ggcgtaatca tggtcatagc tgtttccaat tcttgaagac
     7321 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7381 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7441 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7501 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7561 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7621 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7681 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7741 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     7801 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7861 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7921 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7981 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8041 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8101 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8161 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8221 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8281 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8341 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8401 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8461 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8521 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8581 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8641 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     8701 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8761 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8821 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8881 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8941 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9001 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9061 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9121 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9181 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9241 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9301 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     9361 tttcacaccg catagtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
     9421 attggagctt ggcgtaatca tggtcat
//
