LOCUS       pnmsb91_1_              4381 bp ds-DNA     circular     02-MAY-2023
DEFINITION  .
KEYWORDS    "Plasmid Name:pNMSB91" "alternate name:pMP81" "Sequence"
            "Resistance:Amp" "Gene:ACR1" "Fluo Marker:none" "Notes" "mutation"
            "Location/Freezer"
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        1..31
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    35..57
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     63..83
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    244..260
                     /locus_tag="UAS"
                     /label="UAS"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    263..279
                     /locus_tag="UAS(1)"
                     /label="UAS(1)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    282..298
                     /locus_tag="UAS(2)"
                     /label="UAS(2)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    301..317
                     /locus_tag="UAS(3)"
                     /label="UAS(3)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    320..336
                     /locus_tag="UAS(4)"
                     /label="UAS(4)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    377..393
                     /locus_tag="UAS(5)"
                     /label="UAS(5)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    396..412
                     /locus_tag="UAS(6)"
                     /label="UAS(6)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    415..431
                     /locus_tag="UAS(7)"
                     /label="UAS(7)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    434..450
                     /locus_tag="UAS(8)"
                     /label="UAS(8)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    453..469
                     /locus_tag="UAS(9)"
                     /label="UAS(9)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    510..526
                     /locus_tag="UAS(10)"
                     /label="UAS(10)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    529..545
                     /locus_tag="UAS(11)"
                     /label="UAS(11)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    548..564
                     /locus_tag="UAS(12)"
                     /label="UAS(12)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    567..583
                     /locus_tag="UAS(13)"
                     /label="UAS(13)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    586..602
                     /locus_tag="UAS(14)"
                     /label="UAS(14)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    730..910
                     /locus_tag="delta pes-10"
                     /label="delta pes-10"
                     /ApEinfo_label="delta pes-10"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    940..1824
                     /locus_tag="ACR1 (genebank entry KP171708)"
                     /label="ACR1 (genebank entry KP171708)"
                     /ApEinfo_label="ACR1 (genebank entry KP171708)"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     UTR             1843..2275
                     /locus_tag="let-858 3UTR"
                     /label="let-858 3UTR"
                     /ApEinfo_label="let-858 3UTR"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2637..3296
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      3394..4076
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
       61 CACAGGAAAC AGCTATGACC ATGATTACGC CaagctGTAA GTTTAAACAT GATCTTACTA
      121 ACTAACTATT CTCATTTAAA TTTTCAGAGC TTAAAAATGG CTGAAATCAC TCACAACGAT
      181 GGATACGCTA ACAACTTGGA AATGAAATAA GCTTGCTAGC CCATGATTAC GCCAAGCTAC
      241 GGGCGGAGTA CTGTCCTCCG AGCGGAGTAC TGTCCTCCGA GCGGAGTACT GTCCTCCGAG
      301 CGGAGTACTG TCCTCCGAGC GGAGTACTGT CCTCCGAGCG GAGACTCCCT AGCCCATGAT
      361 TACGCCAAGC TACGGGCGGA GTACTGTCCT CCGAGCGGAG TACTGTCCTC CGAGCGGAGT
      421 ACTGTCCTCC GAGCGGAGTA CTGTCCTCCG AGCGGAGTAC TGTCCTCCGA GCGGAGACTC
      481 CCTAGCCCAT GATTACGCCA AGCTACGGGC GGAGTACTGT CCTCCGAGCG GAGTACTGTC
      541 CTCCGAGCGG AGTACTGTCC TCCGAGCGGA GTACTGTCCT CCGAGCGGAG TACTGTCCTC
      601 CGAGCGGAGA CTCCCTAGGG GCGGCCGCAC TGACTGGGCC GGCCAAAAAT GCATAAGGTT
      661 TTGCTGGCAC TGTTCTTTAT CTTTCTGGCA CCAGCATCCG CACTGGCAGT CTCCGAACCG
      721 GCCTGCAGGT CGATTTTTTG CAAATTACGA GCGTTGTAGG GGGCGGAGCG ATAGGTCCTA
      781 TAGGTTTTGG TATATCATCA TTCATTCATT CATTGGTACA TTCATTTACC CACCTTCCTC
      841 TTTCTGAGCT TCTCTGGAGT TCTGTGCTTC CTTTTTCCCT TATCTTTATA CTGTAATTTT
      901 TAACTTTCAG GCATTGATTG GCGCGCCtct agaggatcca tgagcagcat cacctgtgat
      961 cccgccatct acggcgaatg gagcagggag aaccagttct gcgtggagaa gagcctgatc
     1021 accctggacg gcatcaagta cgtccagctg gtgatggccg tcgtgagcgc ctgtcaggtg
     1081 ttcttcatgg tgaccagagc ccccaaggtg ccctgggaag ccatctacct gcccaccacc
     1141 gagatgatca cctattccct ggccttcacc ggaaacggct acatcagagt ggccaatggc
     1201 aagtacctgc cctgggccag aatggccagc tggctgtgca cctgccctat catgctgggc
     1261 ctggtgagca atatggccct cgtgaagtac aagagcatcc ccctcaaccc tatgatgatc
     1321 gccgcctcca gcatctgcac cgtgttcggc atcaccgcct ccgtggtgct agacccgctg
     1381 cacgtgtggc tgtactgttt catcagcagc atcttcttca tcttcgagat ggtggtggcc
     1441 ttcgccattt tcgccattac catccacgat ttccagacca tcggctcccc catgtccctg
     1501 aaggtggtgg agaggctgaa gctgatgagg atcgtgttct acgtgagctg gatggcctac
     1561 cctatcctgt ggagcttctc cagcaccggc gcctgcatca tgagcgagaa caccagcagc
     1621 gtgctgtacc tgctgggcga cgctctgtgc aagaacacct acggcatcct gctgtgggct
     1681 acaacctggg gcctgctgaa cggcaagtgg gacagggatt acgtgaaggg caggaacgtg
     1741 gacggcaccc tgatgcctga gtacgagcag gacctggaga agggcaacac cgagaggtac
     1801 gaggacgcca gagccggcga gaccTAACCA TACAAGTAAT CCGGATGATC GACGCCAACG
     1861 TCGTTGAATT TTCAAATTTT AAATACTGAA TATTTGTTTT TTTTCCTATT ATTTATTTAT
     1921 TCTCTTTGTG TTTTTTTTCT TGCTTTCTAA AAAATTAATT CAATCCAAAT CTAAACATTT
     1981 TTTTTTCTCT TTCCGTCTCC CAATTCGTAT TCCGCTCCTC TCATCTGAAC ACAATGTGCA
     2041 AGTTTATTTA TCTTCTCGCT TTCATTTCAT TAGGACGTGG GGGGAATTGG TGGAAGGGGG
     2101 AAACACACAA AAGGATGATG GAAATGAAAT AAGGACACAC AATATGCAAC AACATTCAAT
     2161 TCAGAAATAT GGAGGAAGGT TTAAAAGAAA ACATAAAAAT ATATAGAGGA GGAAGGAAAA
     2221 CTAGTAAAAA ATAAGCAAAG AAATTAGGCG AACGATGAGA ATTGTCCTCG CTTGGGCCCA
     2281 AAAGGCCTTT TTTTTTGGCG CCGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA
     2341 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC
     2401 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG
     2461 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC
     2521 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG
     2581 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA
     2641 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC
     2701 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG
     2761 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA
     2821 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG
     2881 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA
     2941 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT
     3001 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT
     3061 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT
     3121 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG
     3181 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA
     3241 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC
     3301 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA
     3361 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT
     3421 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT
     3481 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT
     3541 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC
     3601 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG
     3661 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG
     3721 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT
     3781 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC
     3841 TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG
     3901 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG
     3961 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT
     4021 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT
     4081 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG
     4141 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA
     4201 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC
     4261 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA
     4321 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG
     4381 C     
//
