LOCUS       pnmsb88                 5413 bp ds-DNA     circular     02-MAY-2023
DEFINITION  .
KEYWORDS    "Plasmid Name:pNMSB88" "alternate name:pMP79" "Sequence"
            "Resistance:Amp" "Gene:NL250" "Fluo Marker:Turquoise" "Notes"
            "mutation" "Location/Freezer"
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        1..31
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    35..57
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     63..83
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    244..260
                     /locus_tag="UAS"
                     /label="UAS"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    263..279
                     /locus_tag="UAS(1)"
                     /label="UAS(1)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    282..298
                     /locus_tag="UAS(2)"
                     /label="UAS(2)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    301..317
                     /locus_tag="UAS(3)"
                     /label="UAS(3)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    320..336
                     /locus_tag="UAS(4)"
                     /label="UAS(4)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    377..393
                     /locus_tag="UAS(5)"
                     /label="UAS(5)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    396..412
                     /locus_tag="UAS(6)"
                     /label="UAS(6)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    415..431
                     /locus_tag="UAS(7)"
                     /label="UAS(7)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    434..450
                     /locus_tag="UAS(8)"
                     /label="UAS(8)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    453..469
                     /locus_tag="UAS(9)"
                     /label="UAS(9)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    510..526
                     /locus_tag="UAS(10)"
                     /label="UAS(10)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    529..545
                     /locus_tag="UAS(11)"
                     /label="UAS(11)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    548..564
                     /locus_tag="UAS(12)"
                     /label="UAS(12)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    567..583
                     /locus_tag="UAS(13)"
                     /label="UAS(13)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    586..602
                     /locus_tag="UAS(14)"
                     /label="UAS(14)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    730..910
                     /locus_tag="delta pes-10"
                     /label="delta pes-10"
                     /ApEinfo_label="delta pes-10"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1041..2838
                     /locus_tag="NL250 Ca2+"
                     /label="NL250 Ca2+"
                     /ApEinfo_label="NL250 Ca2+"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1045..1731
                     /locus_tag="turquoise"
                     /label="turquoise"
                     /ApEinfo_label="turquoise"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1732..1737
                     /locus_tag="linker"
                     /label="linker"
                     /ApEinfo_label="linker"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1732..2835
                     /locus_tag="Luc 250 Ca2+"
                     /label="Luc 250 Ca2+"
                     /ApEinfo_label="Luc 250 Ca2+"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Synthetic       1888..1938
                     /locus_tag="SynIVSR"
                     /label="SynIVSR"
                     /ApEinfo_label="SynIVSR"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1972..2520
                     /locus_tag="CaM-M13"
                     /label="CaM-M13"
                     /ApEinfo_label="CaM-M13"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     UTR             2875..3307
                     /locus_tag="let-858 3UTR"
                     /label="let-858 3UTR"
                     /ApEinfo_label="let-858 3UTR"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3669..4328
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      4426..5108
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
       61 CAcaggaaac agctatgacc atgattacgc caagctGTAA GTTTAAACAT GATCTTACTA
      121 ACTAACTATT CTCATTTAAA TTTTCAGAGC TTAAAAATGG CTGAAATCAC TCACAACGAT
      181 GGATACGCTA ACAACTTGGA AATGAAATAA GCTTGCTAGC CCATGATTAC GCCAAGCTAC
      241 GGGCGGAGTA CTGTCCTCCG AGCGGAGTAC TGTCCTCCGA GCGGAGTACT GTCCTCCGAG
      301 CGGAGTACTG TCCTCCGAGC GGAGTACTGT CCTCCGAGCG GAGACTCCCT AGCCCATGAT
      361 TACGCCAAGC TACGGGCGGA GTACTGTCCT CCGAGCGGAG TACTGTCCTC CGAGCGGAGT
      421 ACTGTCCTCC GAGCGGAGTA CTGTCCTCCG AGCGGAGTAC TGTCCTCCGA GCGGAGACTC
      481 CCTAGCCCAT GATTACGCCA AGCTACGGGC GGAGTACTGT CCTCCGAGCG GAGTACTGTC
      541 CTCCGAGCGG AGTACTGTCC TCCGAGCGGA GTACTGTCCT CCGAGCGGAG TACTGTCCTC
      601 CGAGCGGAGA CTCCCTAGGG GCGGCCGCAC TGACTGGGCC GGCCAAAAAT GCATAAGGTT
      661 TTGCTGGCAC TGTTCTTTAT CTTTCTGGCA CCAGCATCCG CACTGGCAGT CTCCGAACCG
      721 GCCTGCAGGT CGATTTTTTG CAAATTACGA GCGTTGTAGG GGGCGGAGCG ATAGGTCCTA
      781 TAGGTTTTGG TATATCATCA TTCATTCATT CATTGGTACA TTCATTTACC CACCTTCCTC
      841 TTTCTGAGCT TCTCTGGAGT TCTGTGCTTC CTTTTTCCCT TATCTTTATA CTGTAATTTT
      901 TAACTTTCAG GCATTGATTG GCGCGCCTCT AGAGGATCCC CGGGATTGGC CAAAGGACCC
      961 AAAGGTATGT TTCGAATGAT ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGCT
     1021 AGCGTCGACG GTACCGGTAG AAAAATGGTG AGCAAAGGAG AAGAATTATT CACGGGAGTT
     1081 GTACCAATCC TCGTCGAGCT GGACGGAGAC GTCAACGGAC ACAAGTTCAG CGTGTCCGGA
     1141 GAGGGAGAGG GAGACGCCAC CTACGGAAAG CTCACCCTCA AGTTCATCTG CACCACCGGA
     1201 AAGCTGCCAG TCCCATGGCC AACCCTCGTC ACCACCCTCT CCTGGGGAGT CCAGTGCTTC
     1261 GCCCGTTACC CAGACCACAT GAAGCAACAC GACTTCTTCA AGTCCGCCAT GCCAGAGGGA
     1321 TACGTCCAAG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGTGCCGAG
     1381 GTCAAGTTCG AGGGAGACAC CCTCGTCAAC CGCATCGAGC TCAAGGGAAT CGACTTCAAG
     1441 GAGGACGGCA ACATCCTCGG ACACAAGCTC GAGTACAACT ACTTCTCCGA CAACGTCTAC
     1501 ATCACCGCCG ACAAGCAAAA GAACGGAATC AAGGCCAACT TCAAGATCCG CCACAACATC
     1561 GAGGACGGAG GAGTCCAACT CGCCGACCAC TACCAACAAA ACACCCCAAT CGGAGACGGC
     1621 CCAGTCCTCC TCCCAGACAA CCACTACCTG TCCACCCAAT CCAAGCTGTC CAAGGACCCA
     1681 AACGAGAAGC GTGACCACAT GGTCCTGCTC GAGTTCGTCA CCGCCGCCGG GGGATTCGAG
     1741 GACTTCGTCG GAGACTGGCG TCAAACAGCC GGATACAACC TCGACCAAGT CCTCGAGCAA
     1801 GGAGGAGTCT CCTCCTTGTT CCAAAACCTC GGAGTCTCCG TCACCCCAAT CCAAAGGATC
     1861 GTCCTCTCCG GAGAGAATGG ACTCAAGGTA AGTTTAAACA TATATATACT AACTAACCCT
     1921 GATTATTTAA ATTTTCAGAT CGACATCCAT GTCATCATCC CATACGAGGG ACCATGGATG
     1981 CACGACCAAC TCACCGAGGA GCAGATCGCC GAGTTCAAGG AGGCCTTCTC CCTCTTCGAC
     2041 AAGGACGGAG ACGGCACCAT CACCACCCGT GAGCTCGGAA CCGTCATGCG TTCCCTTGGA
     2101 CAAAACCCAA CCGAGGCCGA GCTCCAAGAC ATGATCAATG AAGTCGACGC CGACGGAAAC
     2161 GGAACCATCT ACTTCCCTGA GTTCCTCACC ATGATGGCCC GTAAGATGAA GGACACCGAC
     2221 AGCGAGGAGG AGATCCGTGA GGCCTTCCGT GTCTTCGACA AGGACGGGAA CGGATACATC
     2281 TCCGCCGCCC AACTCCGTCA CGTCATGACC AACCTCGGAG TGAAGCTCAC CGACGAGGAG
     2341 GTCGACGAAA TGATACGTGA GGCCGACATC GACGGAGACG GACAAGTAAA CTACGAGGAG
     2401 TTCGAGCAAA TGATGACCGC CAAGGGAGGA GGATCCAAGA GGCGTTGGAA GAAAAACTTC
     2461 ATCGCCGTCT CCGCCGCCAA CCGTTTCAAG AAGATCTCCT CCTCCGGAGC ACTCGAGCTC
     2521 CTCTCCGGAG ACCAAATGGG ACAAATCGAG AAGATTTTCA AGGTCGTCTA CCCAGTCGAC
     2581 GACCACCACT TTAAGGTCAT CCTCCACTAC GGAACCCTCG TCATCGACGG AGTTACCCCA
     2641 AACATGATCG ACTACTTCGG ACGTCCATAC GAGGGAATCG CCGTGTTCGA CGGAAAGAAG
     2701 ATCACCGTCA CCGGAACCCT CTGGAACGGA AACAAAATCA TCGACGAGCG TCTCATCAAC
     2761 CCAGACGGAT CCCTCCTCTT CCGTGTAACC ATCAACGGAG TCACCGGATG GCGTCTCTGC
     2821 GAGCGTATCC TCGCGTAAGG TACCGAATTC GCTAGCCGGC CATACAAGTA ATCCGGATGA
     2881 TCGACGCCAA CGTCGTTGAA TTTTCAAATT TTAAATACTG AATATTTGTT TTTTTTCCTA
     2941 TTATTTATTT ATTCTCTTTG TGTTTTTTTT CTTGCTTTCT AAAAAATTAA TTCAATCCAA
     3001 ATCTAAACAT TTTTTTTTCT CTTTCCGTCT CCCAATTCGT ATTCCGCTCC TCTCATCTGA
     3061 ACACAATGTG CAAGTTTATT TATCTTCTCG CTTTCATTTC ATTAGGACGT GGGGGGAATT
     3121 GGTGGAAGGG GGAAACACAC AAAAGGATGA TGGAAATGAA ATAAGGACAC ACAATATGCA
     3181 ACAACATTCA ATTCAGAAAT ATGGAGGAAG GTTTAAAAGA AAACATAAAA ATATATAGAG
     3241 GAGGAAGGAA AACTAGTAAA AAATAAGCAA AGAAATTAGG CGAACGATGA GAATTGTCCT
     3301 CGCTTGGGCC CAAAAGGCCT TTTTTTTTGG CGCCGACGTC AGGTGGCACT TTTCGGGGAA
     3361 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA
     3421 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC
     3481 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC
     3541 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT
     3601 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT
     3661 TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG
     3721 CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT
     3781 CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG
     3841 CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA
     3901 AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG
     3961 AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA
     4021 TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC
     4081 AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC
     4141 CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA
     4201 TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA
     4261 GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA
     4321 AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC
     4381 ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC
     4441 CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT
     4501 CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC
     4561 CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT
     4621 TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT
     4681 TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG
     4741 CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA
     4801 AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA
     4861 CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG
     4921 GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG
     4981 AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC
     5041 TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA
     5101 ACGCGGCCtt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5161 cgttatcccc tgattctgtg gataaccgta ttaccgccTT TGAGTGAGCT GATACCGCTC
     5221 GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA
     5281 TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT
     5341 TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT
     5401 AGGCACCCCA GGC    
//
