LOCUS       pnmsb109                6138 bp ds-DNA     circular     02-MAY-2023
DEFINITION  .
KEYWORDS    "Plasmid Name:pNMSB109" "alternate name:pMP98" "Sequence"
            "Resistance:Amp" "Gene:CaMBI" "Fluo Marker:cyOFP1" "Notes"
            "mutation" "Location/Freezer"
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     promoter        1..31
                     /locus_tag="lac promoter"
                     /label="lac promoter"
                     /ApEinfo_label="lac promoter"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    35..57
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     63..83
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    244..260
                     /locus_tag="UAS"
                     /label="UAS"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    263..279
                     /locus_tag="UAS(1)"
                     /label="UAS(1)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    282..298
                     /locus_tag="UAS(2)"
                     /label="UAS(2)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    301..317
                     /locus_tag="UAS(3)"
                     /label="UAS(3)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    320..336
                     /locus_tag="UAS(4)"
                     /label="UAS(4)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    377..393
                     /locus_tag="UAS(5)"
                     /label="UAS(5)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    396..412
                     /locus_tag="UAS(6)"
                     /label="UAS(6)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    415..431
                     /locus_tag="UAS(7)"
                     /label="UAS(7)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    434..450
                     /locus_tag="UAS(8)"
                     /label="UAS(8)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    453..469
                     /locus_tag="UAS(9)"
                     /label="UAS(9)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    510..526
                     /locus_tag="UAS(10)"
                     /label="UAS(10)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    529..545
                     /locus_tag="UAS(11)"
                     /label="UAS(11)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    548..564
                     /locus_tag="UAS(12)"
                     /label="UAS(12)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    567..583
                     /locus_tag="UAS(13)"
                     /label="UAS(13)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    586..602
                     /locus_tag="UAS(14)"
                     /label="UAS(14)"
                     /ApEinfo_label="UAS"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    730..910
                     /locus_tag="delta pes-10"
                     /label="delta pes-10"
                     /ApEinfo_label="delta pes-10"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1041..3563
                     /locus_tag="CaMBI"
                     /label="CaMBI"
                     /ApEinfo_label="CaMBI"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Synthetic       1884..1934
                     /locus_tag="SynIVSR"
                     /label="SynIVSR"
                     /ApEinfo_label="SynIVSR"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     Synthetic       2721..2771
                     /locus_tag="SynIVSS"
                     /label="SynIVSS"
                     /ApEinfo_label="SynIVSS"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     UTR             3600..4032
                     /locus_tag="let-858 3UTR"
                     /label="let-858 3UTR"
                     /ApEinfo_label="let-858 3UTR"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             4394..5053
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      5151..5833
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA
       61 CAcaggaaac agctatgacc atgattacgc caagctGTAA GTTTAAACAT GATCTTACTA
      121 ACTAACTATT CTCATTTAAA TTTTCAGAGC TTAAAAATGG CTGAAATCAC TCACAACGAT
      181 GGATACGCTA ACAACTTGGA AATGAAATAA GCTTGCTAGC CCATGATTAC GCCAAGCTAC
      241 GGGCGGAGTA CTGTCCTCCG AGCGGAGTAC TGTCCTCCGA GCGGAGTACT GTCCTCCGAG
      301 CGGAGTACTG TCCTCCGAGC GGAGTACTGT CCTCCGAGCG GAGACTCCCT AGCCCATGAT
      361 TACGCCAAGC TACGGGCGGA GTACTGTCCT CCGAGCGGAG TACTGTCCTC CGAGCGGAGT
      421 ACTGTCCTCC GAGCGGAGTA CTGTCCTCCG AGCGGAGTAC TGTCCTCCGA GCGGAGACTC
      481 CCTAGCCCAT GATTACGCCA AGCTACGGGC GGAGTACTGT CCTCCGAGCG GAGTACTGTC
      541 CTCCGAGCGG AGTACTGTCC TCCGAGCGGA GTACTGTCCT CCGAGCGGAG TACTGTCCTC
      601 CGAGCGGAGA CTCCCTAGGG GCGGCCGCAC TGACTGGGCC GGCCAAAAAT GCATAAGGTT
      661 TTGCTGGCAC TGTTCTTTAT CTTTCTGGCA CCAGCATCCG CACTGGCAGT CTCCGAACCG
      721 GCCTGCAGGT CGATTTTTTG CAAATTACGA GCGTTGTAGG GGGCGGAGCG ATAGGTCCTA
      781 TAGGTTTTGG TATATCATCA TTCATTCATT CATTGGTACA TTCATTTACC CACCTTCCTC
      841 TTTCTGAGCT TCTCTGGAGT TCTGTGCTTC CTTTTTCCCT TATCTTTATA CTGTAATTTT
      901 TAACTTTCAG GCATTGATTG GCGCGCCTCT AGAGGATCCC CGGGATTGGC CAAAGGACCC
      961 AAAGGTATGT TTCGAATGAT ACTAACATAA CATAGAACAT TTTCAGGAGG ACCCTTGGCT
     1021 AGCGTCGACG GTACCGGTAG ATGGTTTCTA AGGGAGAAGA ACTTATCAAG GAGAACATGC
     1081 GCTCTAAGCT TTACCTTGAG GGATCTGTTA ACGGACATCA ATTCAAGTGC ACTCACGAGG
     1141 GAGAAGGAAA GCCATATGAA GGAAAGCAAA CTAACCGCAT CAAGGTTGTT GAAGGAGGAC
     1201 CACTTCCATT CGCTTTCGAC ATCCTTGCTA CTCATTTCAT GTACGGATCT AAGGTTTTCA
     1261 TTAAGTACCC AGCTGATCTT CCAGACTACT TCAAGCAATC TTTCCCAGAA GGATTCACTT
     1321 GGGAGCGTGT TATGGTTTTC GAGGATGGAG GAGTCCTTAC CGCTACCCAA GATACCTCCC
     1381 TTCAAGATGG AGAGCTTATC TACAACGTTA AGGTTCGTGG AGTTAATTTC CCAGCTAACG
     1441 GACCAGTTAT GCAAAAGAAG ACTCTTGGAT GGGAGCCATC TACTGAGACC ATGTACCCAG
     1501 CCGATGGAGG ACTTGAGGGA CGCTGCGACA AGGCTCTTAA GCTTGTTGGA GGAGGACACC
     1561 TTCACGTTAA CTTCAAGACT ACTTACAAGT CTAAAAAGCC AGTTAAGATG CCAGGAGTTC
     1621 ATTACGTCGA TCGTCGCCTT GAGCGTATCA AGGAGGCTGA TAACGAGACT TACGTTGAAC
     1681 AATACGAGCA CGCCGTCGCT CGTTACTCTA ACCTTGGAGG AGGATTCACT CTTGAGGACT
     1741 TCGTCGGAGA TTGGCGCCAA ACCGCTGGAT ACAACCTTGA TCAAGTTCTC GAGCAAGGAG
     1801 GAGTTTCTTC CCTTTTCCAA AACCTTGGAG TTTCTGTTAC CCCAATCCAA CGCATCGTTC
     1861 TTTCTGGAGA GAACGGACTT AAGgtaagtt taaacatata tatactaact aaccctgatt
     1921 atttaaattt tcagATCGAT ATCCACGTTA TCATCCCATA CGAGGGACTT TCTGGAGATC
     1981 AGATGGGACA AATCGAGAAG ATCTTCAAGG TTGTTTACCC AGTCGACGAC CACCACTTCA
     2041 AGGTTATCCT TCACTACGGA ACCCTTGTTA TCGATGGAGT CACTCCAAAC ATGATTGATT
     2101 ACTTCGGACG CCCATACGAG GGAATCGCTG TTTTCGATGG AAAGAAGATC ACCGTTACTG
     2161 GAACCCTTAT CATGCACGAT CAACTTACTG AGGAACAAAT CGCTGAATTC AAGGAGGCTT
     2221 TCTCCCTTTT CGATAAGGAC GGAGATGGAA CTATCACTAC CAAGGAGCTC GGAACCGTCA
     2281 TGCGTTCTCT CGGACAAAAC CCAACTGAGG CTGAGCTTCA AGACATGATC AACGAGGTTG
     2341 ACGCCGACGG AAACGGAACT ATCTACTTCC CAGAGTTCCT CACCATGATG GCCCGCAAGA
     2401 TGAAGGATAC CGATTCTGAG GAGGAGATCC GCGAGGCTTT CCGTGTCTTC GATAAGGACG
     2461 GAAACGGATA CATTTCCGCC GCCCAACTTC GTCACGTTAT GACTAACCTT GGAGAGAAGC
     2521 TTACCGACGA GGAGGTCGAC GAGATGATCC GTGAGGCTGA CATCGACGGA GACGGACAAG
     2581 TTAACTACGA GGAGTTCGTT CAAATGATGA CTGCTAAGGG AAAGCGCCGT TGGAAGAAGA
     2641 ACTTCATCGC TGTCTCCGCC GCTAACCGCT TCAAGAAGAT CTCTTCTTCC GGAGCCCTTG
     2701 AACTTTGGAA CGGAAACAAG gtaagtttaa acagttcggt actaactaac catacatatt
     2761 taaattttca gATCATTGAT GAGCGTCTTA TCAACCCAGA TGGATCCCTT CTTTTCCGCG
     2821 TTACTATCAA CGGAGTTACT GGATGGCGCC TTTGCGAGCG TATCCTTGCT CGCCACGAGC
     2881 TTATCAAGGA GAACATGCGC TCTAAGCTTT ACCTTGAGGG ATCTGTTAAC GGACACCAGT
     2941 TCAAGTGCAC CCACGAAGGA GAGGGAAAGC CATATGAGGG AAAGCAAACT AACCGCATCA
     3001 AGGTTGTTGA GGGAGGACCA CTTCCATTCG CTTTCGACAT CCTTGCCACT CACTTCATGT
     3061 ACGGATCCAA GGTTTTCATC AAGTACCCAG CTGACCTTCC AGATTACTTC AAGCAATCTT
     3121 TCCCAGAGGG ATTCACCTGG GAGCGTGTTA TGGTTTTCGA GGACGGAGGA GTCCTTACCG
     3181 CCACCCAAGA CACCTCCCTC CAAGACGGAG AGCTTATCTA CAACGTTAAG GTTCGTGGAG
     3241 TTAACTTCCC AGCTAACGGA CCAGTTATGC AGAAGAAGAC TCTTGGATGG GAGCCATCCA
     3301 CTGAGACCAT GTACCCAGCT GACGGAGGAC TTGAGGGACG CTGCGACAAG GCCCTCAAGC
     3361 TTGTTGGAGG AGGACACCTT CACGTTAACT TCAAGACCAC TTACAAGTCT AAGAAACCAG
     3421 TTAAGATGCC AGGAGTTCAC TACGTCGACC GCCGCCTTGA GCGTATCAAG GAGGCCGACA
     3481 ACGAGACTTA CGTTGAGCAA TACGAGCACG CTGTCGCTCG TTACTCTAAC CTTGGAGGAG
     3541 GAATGGACGA GCTTTACAAG TAAGGTACCG AATTCGCTAG CCGGCCATAC AAGTAATCCG
     3601 GATGATCGAC GCCAACGTCG TTGAATTTTC AAATTTTAAA TACTGAATAT TTGTTTTTTT
     3661 TCCTATTATT TATTTATTCT CTTTGTGTTT TTTTTCTTGC TTTCTAAAAA ATTAATTCAA
     3721 TCCAAATCTA AACATTTTTT TTTCTCTTTC CGTCTCCCAA TTCGTATTCC GCTCCTCTCA
     3781 TCTGAACACA ATGTGCAAGT TTATTTATCT TCTCGCTTTC ATTTCATTAG GACGTGGGGG
     3841 GAATTGGTGG AAGGGGGAAA CACACAAAAG GATGATGGAA ATGAAATAAG GACACACAAT
     3901 ATGCAACAAC ATTCAATTCA GAAATATGGA GGAAGGTTTA AAAGAAAACA TAAAAATATA
     3961 TAGAGGAGGA AGGAAAACTA GTAAAAAATA AGCAAAGAAA TTAGGCGAAC GATGAGAATT
     4021 GTCCTCGCTT GGGCCCAAAA GGCCTTTTTT TTTGGCGCCG ACGTCAGGTG GCACTTTTCG
     4081 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC
     4141 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG
     4201 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT
     4261 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT
     4321 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA
     4381 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT
     4441 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA
     4501 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG
     4561 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG
     4621 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG
     4681 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT
     4741 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG
     4801 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC
     4861 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG
     4921 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC
     4981 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT
     5041 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA
     5101 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA
     5161 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG
     5221 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
     5281 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
     5341 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA
     5401 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
     5461 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
     5521 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
     5581 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC
     5641 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
     5701 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
     5761 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
     5821 CAGCAACGCG GCCtttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     5881 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccTTTGAGT GAGCTGATAC
     5941 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG
     6001 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA
     6061 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC
     6121 TCATTAGGCA CCCCAGGC    
//
