LOCUS       Exported                7333 bp ds-DNA     circular SYN 22-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..636
                     /locus_tag="5' LTR"
                     /label=5' LTR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    1..454
                     /locus_tag="U3"
                     /label=U3
                     /note="U3"
     misc_feature    455..551
                     /locus_tag="R"
                     /label=R
                     /note="R"
     misc_feature    552..636
                     /locus_tag="U5"
                     /label=U5
                     /note="U5"
     misc_feature    697..806
                     /locus_tag="HIV-PSI"
                     /label=HIV-PSI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             790..1128
                     /locus_tag="HIV-1 gag (fragment)"
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=22.6"
                     /note="/other=CDS"
     misc_feature    1303..1536
                     /locus_tag="RRE"
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             1913..2013
                     /locus_tag="Rev (fragment)"
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
     misc_feature    2058..2175
                     /locus_tag="cPPT/CTS"
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    2233..2238
                     /locus_tag="SpeI"
                     /label=SpeI
     enhancer        2295..2598
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        2599..2802
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        2804..2823
                     /locus_tag="TetO-1"
                     /label=TetO-1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     promoter        2825..2844
                     /locus_tag="TetO-2"
                     /label=TetO-2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     misc_feature    2949..2956
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    2952..2961
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    2958..2960
                     /locus_tag="Initiator Met, translation start"
                     /label=Initiator Met, translation start
                     /note="Initiator Met, translation start"
     CDS             2964..3647
                     /locus_tag="EGFP"
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=99.1"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    3717..3722
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    3729..3734
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    3747..3752
                     /locus_tag="AgeI"
                     /label=AgeI
     misc_feature    3759..3764
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    3768..3773
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    3775..4363
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    4163
                     /locus_tag="exchange"
                     /label=exchange
     misc_feature    4416..4435
                     /locus_tag="cPPT/CTS (fragment)"
                     /label=cPPT/CTS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.9"
                     /note="/other=misc_feature"
     LTR             4437..4691
                     /locus_tag="3' LTR (truncated)"
                     /label=3' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    4437..4511
                     /locus_tag="dU3 fragment, deletion of part of U3"
                     /label=dU3 fragment, deletion of part of U3
                     /note="dU3 fragment, deletion of part of U3"
     misc_feature    4512..4608
                     /locus_tag="R(1)"
                     /label=R
                     /label=R(1)
                     /note="R"
     misc_feature    4609..4691
                     /locus_tag="U5(1)"
                     /label=U5
                     /label=U5(1)
                     /note="U5"
     promoter        4824..4928
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             4929..5789
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      5960..6548
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             6731..6785
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     rep_origin      6791..6884
                     /locus_tag="SV40 ori (fragment)"
                     /label=SV40 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=69.1"
                     /note="/other=rep_origin"
     CDS             6909..6946
                     /locus_tag="lacI (fragment)(1)"
                     /label=lacI (fragment)
                     /label=lacI (fragment)(1)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.5"
                     /note="/other=CDS"
     protein_bind    6959..6980
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        6995..7025
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    7033..7049
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    7083..7166
                     /locus_tag="SV40 poly(A) signal (fragment)"
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=62.2"
                     /note="/other=polyA_signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gacgcgttga
     2221 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2281 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2341 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2401 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2461 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2521 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2581 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2641 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2701 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2761 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     2821 gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     2941 cggctagagc ggccgccatg gtgtctaaag gtgaagaact ttttacgggt gtcgtcccca
     3001 tcctggttga attggacgga gacgtgaatg gccataaatt ttcagtgagc ggtgaaggag
     3061 agggagacgc cacttacggt aaacttacct tgaaatttat ctgcactacc gggaaactgc
     3121 ccgtaccctg gccgacgctc gtaacaacgc ttacctacgg cgtgcaatgt tttagcagat
     3181 acccagacca tatgaagcag cacgattttt tcaagagtgc aatgcctgag gggtacgtac
     3241 aggaaaggac gatctttttc aaagacgatg gtaattataa aacgcgggct gaggttaaat
     3301 ttgaaggaga cacgctcgtg aatcgaatag agctgaaggg aatcgacttc aaggaagacg
     3361 gtaacatcct gggccataaa ctcgaatata attacaattc acacaacgtc tatatcatgg
     3421 cagataaaca gaagaatggc atcaaagtca attttaagat ccggcataat atcgaggacg
     3481 gatcagtaca gcttgccgac cattaccaac agaatacacc gataggagac ggtcccgtcc
     3541 tcttgccaga taatcactat ttgagcacac aatccgctct ttctaaagat ccgaatgaaa
     3601 agcgcgatca tatggtcttg aaagagttcg tcactgccgc cggcataacc ggatctggca
     3661 gtggatcagg atctggtagt ggttgtggta gcggatgtgg aagtggcagt tctagtggat
     3721 ccggttctgc tagcggttca ggttctaccg gtggatcatc tagataactc gagtaatcaa
     3781 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3841 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3901 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3961 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4021 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4081 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4141 actgacaatt ccgtggtgtt gttggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4201 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4261 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4321 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggtac ctttaagacc
     4381 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4441 agggctaatt cactcccaac gaagacaaga tcacctgcag gacaggcgcg ccctgctttt
     4501 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     4561 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     4621 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     4681 aatctctagc acccgggcga ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc
     4741 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4801 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4861 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4921 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4981 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5041 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5101 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5161 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5221 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5281 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5341 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5401 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5461 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5521 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5581 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5641 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5701 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5761 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5821 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5881 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5941 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6001 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6061 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6121 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6181 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6241 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6301 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6361 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6421 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6481 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6541 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6601 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6661 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6721 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6781 gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     6841 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6901 gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     6961 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7021 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta
     7081 cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7141 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     7201 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     7261 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     7321 caaacttagt agt
//
