LOCUS       Exported                7336 bp ds-DNA     circular SYN 22-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..636
                     /locus_tag="5' LTR"
                     /label=5' LTR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    1..454
                     /locus_tag="U3"
                     /label=U3
                     /note="U3"
     misc_feature    455..551
                     /locus_tag="R"
                     /label=R
                     /note="R"
     misc_feature    552..636
                     /locus_tag="U5"
                     /label=U5
                     /note="U5"
     misc_feature    697..806
                     /locus_tag="HIV-PSI"
                     /label=HIV-PSI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             790..1128
                     /locus_tag="HIV-1 gag (fragment)"
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=22.6"
                     /note="/other=CDS"
     misc_feature    1303..1536
                     /locus_tag="RRE"
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             1913..2013
                     /locus_tag="Rev (fragment)"
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
     misc_feature    2058..2175
                     /locus_tag="cPPT/CTS"
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    2233..2238
                     /locus_tag="SpeI"
                     /label=SpeI
     enhancer        2295..2598
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        2599..2802
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        2804..2823
                     /locus_tag="TetO-1"
                     /label=TetO-1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     promoter        2825..2844
                     /locus_tag="TetO-2"
                     /label=TetO-2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     misc_feature    2949..2956
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    2952..2961
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    2958..2960
                     /locus_tag="Initiator Met"
                     /label=Initiator Met
     misc_feature    2961..2966
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    2973..2978
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    2991..2996
                     /locus_tag="AgeI"
                     /label=AgeI
     misc_feature    3003..3008
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    3078..3767
                     /locus_tag="EGFP"
                     /label=EGFP
     misc_feature    3771..3776
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    3778..4366
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    4166
                     /locus_tag="exchange"
                     /label=exchange
     misc_feature    4419..4438
                     /locus_tag="cPPT/CTS (fragment)"
                     /label=cPPT/CTS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.9"
                     /note="/other=misc_feature"
     LTR             4440..4694
                     /locus_tag="3' LTR (truncated)"
                     /label=3' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    4440..4514
                     /locus_tag="dU3 fragment, deletion of part of U3"
                     /label=dU3 fragment, deletion of part of U3
                     /note="dU3 fragment, deletion of part of U3"
     misc_feature    4515..4611
                     /locus_tag="R(1)"
                     /label=R
                     /label=R(1)
                     /note="R"
     misc_feature    4612..4694
                     /locus_tag="U5(1)"
                     /label=U5
                     /label=U5(1)
                     /note="U5"
     promoter        4827..4931
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             4932..5792
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      5963..6551
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             6734..6788
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     rep_origin      6794..6887
                     /locus_tag="SV40 ori (fragment)"
                     /label=SV40 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=69.1"
                     /note="/other=rep_origin"
     CDS             6912..6949
                     /locus_tag="lacI (fragment)(1)"
                     /label=lacI (fragment)
                     /label=lacI (fragment)(1)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.5"
                     /note="/other=CDS"
     protein_bind    6962..6983
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        6998..7028
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    7036..7052
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    7086..7169
                     /locus_tag="SV40 poly(A) signal (fragment)"
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=62.2"
                     /note="/other=polyA_signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gacgcgttga
     2221 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2281 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2341 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2401 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2461 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2521 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2581 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2641 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2701 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2761 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     2821 gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     2941 cggctagagc ggccgccatg ggatccggtt ctgctagcgg ttcaggttct accggtggat
     3001 catctagaac tagcggaagt ggatctggat caggaagtgg ttctggatca ggtagtggtt
     3061 ctggtagtgg atcaatggtg tctaaaggtg aagaactttt tacgggtgtc gtccccatcc
     3121 tggttgaatt ggacggagac gtgaatggcc ataaattttc agtgagcggt gaaggagagg
     3181 gagacgccac ttacggtaaa cttaccttga aatttatctg cactaccggg aaactgcccg
     3241 taccctggcc gacgctcgta acaacgctta cctacggcgt gcaatgtttt agcagatacc
     3301 cagaccatat gaagcagcac gattttttca agagtgcaat gcctgagggg tacgtacagg
     3361 aaaggacgat ctttttcaaa gacgatggta attataaaac gcgggctgag gttaaatttg
     3421 aaggagacac gctcgtgaat cgaatagagc tgaagggaat cgacttcaag gaagacggta
     3481 acatcctggg ccataaactc gaatataatt acaattcaca caacgtctat atcatggcag
     3541 ataaacagaa gaatggcatc aaagtcaatt ttaagatccg gcataatatc gaggacggat
     3601 cagtacagct tgccgaccat taccaacaga atacaccgat aggagacggt cccgtcctct
     3661 tgccagataa tcactatttg agcacacaat ccgctctttc taaagatccg aatgaaaagc
     3721 gcgatcatat ggtcttgaaa gagttcgtca ctgccgccgg cataacctaa ctcgagtaat
     3781 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3841 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3901 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3961 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4021 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4081 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4141 ggcactgaca attccgtggt gttgttgggg aaatcatcgt cctttccttg gctgctcgcc
     4201 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4261 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4321 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     4381 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4441 ggaagggcta attcactccc aacgaagaca agatcacctg caggacaggc gcgccctgct
     4501 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4561 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4621 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4681 gaaaatctct agcacccggg cgattaagga aagggctaga tcattcttga agacgaaagg
     4741 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4801 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4861 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4921 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4981 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5041 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5101 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5161 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5221 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5281 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5341 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5401 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5461 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5521 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5581 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5641 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5701 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5761 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5821 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5881 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5941 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6001 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6061 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6121 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6181 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6241 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6301 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6361 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6421 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6481 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6541 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6601 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6661 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6721 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6781 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6841 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6901 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6961 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7021 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
     7081 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7141 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     7201 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     7261 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     7321 ctacaaactt agtagt
//
