LOCUS       Exported                7360 bp ds-DNA     circular SYN 22-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..636
                     /locus_tag="5' LTR"
                     /label=5' LTR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    1..454
                     /locus_tag="U3"
                     /label=U3
                     /note="U3"
     misc_feature    455..551
                     /locus_tag="R"
                     /label=R
                     /note="R"
     misc_feature    552..636
                     /locus_tag="U5(1)"
                     /label=U5
                     /label=U5(1)
                     /note="U5"
     misc_feature    697..806
                     /locus_tag="HIV-PSI"
                     /label=HIV-PSI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             790..1128
                     /locus_tag="HIV-1 gag (fragment)"
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=22.6"
                     /note="/other=CDS"
     misc_feature    1303..1536
                     /locus_tag="RRE"
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             1913..2013
                     /locus_tag="Rev (fragment)"
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
     misc_feature    2058..2175
                     /locus_tag="cPPT/CTS"
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    2233..2238
                     /locus_tag="SpeI"
                     /label=SpeI
     enhancer        2295..2598
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        2599..2802
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        2804..2823
                     /locus_tag="TetO-1"
                     /label=TetO-1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     promoter        2825..2844
                     /locus_tag="TetO-2"
                     /label=TetO-2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     misc_feature    2949..2956
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    2952..2961
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    2958..2960
                     /locus_tag="Initiator Met"
                     /label=Initiator Met
     misc_feature    2961..2966
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    2973..2978
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    2991..2996
                     /locus_tag="AgeI"
                     /label=AgeI
     misc_feature    3003..3008
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    3021..3044
                     /locus_tag="3C protease cleavage site"
                     /label=3C protease cleavage site
     misc_feature    3102..3791
                     /locus_tag="EGFP"
                     /label=EGFP
     misc_feature    3795..3800
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    3802..4390
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    4190
                     /locus_tag="exchange"
                     /label=exchange
     misc_feature    4443..4462
                     /locus_tag="cPPT/CTS (fragment)"
                     /label=cPPT/CTS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.9"
                     /note="/other=misc_feature"
     LTR             4464..4718
                     /locus_tag="3' LTR (truncated)"
                     /label=3' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    4464..4538
                     /locus_tag="dU3 fragment, deletion of part of U3"
                     /label=dU3 fragment, deletion of part of U3
                     /note="dU3 fragment, deletion of part of U3"
     misc_feature    4539..4635
                     /locus_tag="R(1)"
                     /label=R
                     /label=R(1)
                     /note="R"
     misc_feature    4636..4718
                     /locus_tag="U5"
                     /label=U5
                     /note="U5"
     promoter        4851..4955
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             4956..5816
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      5987..6575
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             6758..6812
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     rep_origin      6818..6911
                     /locus_tag="SV40 ori (fragment)"
                     /label=SV40 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=69.1"
                     /note="/other=rep_origin"
     CDS             6936..6973
                     /locus_tag="lacI (fragment)(1)"
                     /label=lacI (fragment)
                     /label=lacI (fragment)(1)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.5"
                     /note="/other=CDS"
     protein_bind    6986..7007
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        7022..7052
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    7060..7076
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    7110..7193
                     /locus_tag="SV40 poly(A) signal (fragment)"
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=62.2"
                     /note="/other=polyA_signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gacgcgttga
     2221 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2281 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2341 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2401 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2461 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2521 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2581 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2641 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2701 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2761 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     2821 gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     2941 cggctagagc ggccgccatg ggatccggtt ctgctagcgg ttcaggttct accggtggat
     3001 catctagaac tagcggtagc ttagaagttt tgtttcaagg tccaggatct ggatctggaa
     3061 gtggttctgg atcaggtagt ggttctggta gtggatcaat ggtgtctaaa ggtgaagaac
     3121 tttttacggg tgtcgtcccc atcctggttg aattggacgg agacgtgaat ggccataaat
     3181 tttcagtgag cggtgaagga gagggagacg ccacttacgg taaacttacc ttgaaattta
     3241 tctgcactac cgggaaactg cccgtaccct ggccgacgct cgtaacaacg cttacctacg
     3301 gcgtgcaatg ttttagcaga tacccagacc atatgaagca gcacgatttt ttcaagagtg
     3361 caatgcctga ggggtacgta caggaaagga cgatcttttt caaagacgat ggtaattata
     3421 aaacgcgggc tgaggttaaa tttgaaggag acacgctcgt gaatcgaata gagctgaagg
     3481 gaatcgactt caaggaagac ggtaacatcc tgggccataa actcgaatat aattacaatt
     3541 cacacaacgt ctatatcatg gcagataaac agaagaatgg catcaaagtc aattttaaga
     3601 tccggcataa tatcgaggac ggatcagtac agcttgccga ccattaccaa cagaatacac
     3661 cgataggaga cggtcccgtc ctcttgccag ataatcacta tttgagcaca caatccgctc
     3721 tttctaaaga tccgaatgaa aagcgcgatc atatggtctt gaaagagttc gtcactgccg
     3781 ccggcataac ctaactcgag taatcaacct ctggattaca aaatttgtga aagattgact
     3841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3901 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3961 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     4021 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     4081 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     4141 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtt ggggaaatca
     4201 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     4261 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     4321 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     4381 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     4441 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatca
     4501 cctgcaggac aggcgcgccc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4561 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4621 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4681 cctcagaccc ttttagtcag tgtggaaaat ctctagcacc cgggcgatta aggaaagggc
     4741 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     4801 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     5221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5461 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6781 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
     6841 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6901 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
     6961 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7021 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7081 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
     7141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     7201 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     7261 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
     7321 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
