LOCUS       Exported                7672 bp ds-DNA     circular SYN 22-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7672
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..636
                     /locus_tag="5' LTR"
                     /label=5' LTR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    1..454
                     /locus_tag="U3"
                     /label=U3
                     /note="U3"
     misc_feature    455..551
                     /locus_tag="R"
                     /label=R
                     /note="R"
     misc_feature    552..636
                     /locus_tag="U5(1)"
                     /label=U5
                     /label=U5(1)
                     /note="U5"
     misc_feature    697..806
                     /locus_tag="HIV-PSI"
                     /label=HIV-PSI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             790..1128
                     /locus_tag="HIV-1 gag (fragment)"
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=22.6"
                     /note="/other=CDS"
     misc_feature    1303..1536
                     /locus_tag="RRE"
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             1913..2013
                     /locus_tag="Rev (fragment)"
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
     misc_feature    2058..2175
                     /locus_tag="cPPT/CTS"
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    2233..2238
                     /locus_tag="SpeI"
                     /label=SpeI
     enhancer        2295..2598
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        2599..2802
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        2804..2823
                     /locus_tag="TetO-1"
                     /label=TetO-1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     promoter        2825..2844
                     /locus_tag="TetO-2"
                     /label=TetO-2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     misc_feature    2949..2956
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    2952..2961
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    2958..2960
                     /locus_tag="Initiator Met"
                     /label=Initiator Met
     misc_feature    2961..2966
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    2973..2978
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    2991..2996
                     /locus_tag="AgeI"
                     /label=AgeI
     misc_feature    3003..3008
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    3021..3044
                     /locus_tag="3C protease cleavage site"
                     /label=3C protease cleavage site
     misc_feature    3057..3344
                     /locus_tag="SumoEu1"
                     /label=SumoEu1
                     /note="SumoEu1 Vera et al. 2019"
     misc_feature    3342..3344
                     /locus_tag="SUMO protease cleavage site"
                     /label=SUMO protease cleavage site
     misc_feature    3414..4103
                     /locus_tag="EGFP"
                     /label=EGFP
     misc_feature    4107..4112
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    4114..4702
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    4502
                     /locus_tag="exchange"
                     /label=exchange
     misc_feature    4755..4774
                     /locus_tag="cPPT/CTS (fragment)"
                     /label=cPPT/CTS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.9"
                     /note="/other=misc_feature"
     LTR             4776..5030
                     /locus_tag="3' LTR (truncated)"
                     /label=3' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    4776..4850
                     /locus_tag="dU3 fragment, deletion of part of U3"
                     /label=dU3 fragment, deletion of part of U3
                     /note="dU3 fragment, deletion of part of U3"
     misc_feature    4851..4947
                     /locus_tag="R(1)"
                     /label=R
                     /label=R(1)
                     /note="R"
     misc_feature    4948..5030
                     /locus_tag="U5"
                     /label=U5
                     /note="U5"
     promoter        5163..5267
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5268..6128
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      6299..6887
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             7070..7124
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     rep_origin      7130..7223
                     /locus_tag="SV40 ori (fragment)"
                     /label=SV40 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=69.1"
                     /note="/other=rep_origin"
     CDS             7248..7285
                     /locus_tag="lacI (fragment)(1)"
                     /label=lacI (fragment)
                     /label=lacI (fragment)(1)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.5"
                     /note="/other=CDS"
     protein_bind    7298..7319
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        7334..7364
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    7372..7388
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    7422..7505
                     /locus_tag="SV40 poly(A) signal (fragment)"
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=62.2"
                     /note="/other=polyA_signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gacgcgttga
     2221 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2281 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2341 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2401 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2461 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2521 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2581 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2641 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2701 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2761 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     2821 gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     2941 cggctagagc ggccgccatg ggatccggtt ctgctagcgg ttcaggttct accggtggat
     3001 catctagaac tagcggtagc ttagaagttt tgtttcaagg tccaggtagc ggatcttctg
     3061 ctgcgggtgg cgaagaagat aagaaaccgg caggtggcga aggtggcggt gcccatatca
     3121 acctgaaagt gaaaggtcaa gacggcaacg aagtcttttt ccgcatcaaa cgttctaccc
     3181 agctgaaaaa gctgatgaac gcatactgtg accgtcagtc tgtagacatg aaggcaattg
     3241 ctttcctctt taagggtcgt cgcctacgtg cggaaaggac cccggatgaa ctggaaatgg
     3301 aagatggcga cgaaatcgac gcaatgctgc atcagactgg tggcggatct ggaagtggat
     3361 ctggatcagg aagtggttct ggatcaggta gtggttctgg tagtggatca atggtgtcta
     3421 aaggtgaaga actttttacg ggtgtcgtcc ccatcctggt tgaattggac ggagacgtga
     3481 atggccataa attttcagtg agcggtgaag gagagggaga cgccacttac ggtaaactta
     3541 ccttgaaatt tatctgcact accgggaaac tgcccgtacc ctggccgacg ctcgtaacaa
     3601 cgcttaccta cggcgtgcaa tgttttagca gatacccaga ccatatgaag cagcacgatt
     3661 ttttcaagag tgcaatgcct gaggggtacg tacaggaaag gacgatcttt ttcaaagacg
     3721 atggtaatta taaaacgcgg gctgaggtta aatttgaagg agacacgctc gtgaatcgaa
     3781 tagagctgaa gggaatcgac ttcaaggaag acggtaacat cctgggccat aaactcgaat
     3841 ataattacaa ttcacacaac gtctatatca tggcagataa acagaagaat ggcatcaaag
     3901 tcaattttaa gatccggcat aatatcgagg acggatcagt acagcttgcc gaccattacc
     3961 aacagaatac accgatagga gacggtcccg tcctcttgcc agataatcac tatttgagca
     4021 cacaatccgc tctttctaaa gatccgaatg aaaagcgcga tcatatggtc ttgaaagagt
     4081 tcgtcactgc cgccggcata acctaactcg agtaatcaac ctctggatta caaaatttgt
     4141 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4201 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4261 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     4321 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     4381 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     4441 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     4501 ttggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4561 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4621 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4681 tccctttggg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt
     4741 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     4801 aagacaagat cacctgcagg acaggcgcgc cctgcttttt gcttgtactg ggtctctctg
     4861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca cccgggcgat
     5041 taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
     5101 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     5161 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5221 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5281 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5341 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5401 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5461 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     5521 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5581 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5641 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5701 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5761 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5821 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5881 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5941 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6001 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6061 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6121 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6181 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6241 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6301 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6361 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6421 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6481 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6541 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6601 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6661 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6721 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6781 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6841 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6901 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6961 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7021 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7081 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact
     7141 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7201 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt
     7261 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7321 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7381 gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat
     7441 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7501 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     7561 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     7621 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
