LOCUS       Exported                7819 bp ds-DNA     circular SYN 22-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 3, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..636
                     /locus_tag="5' LTR"
                     /label=5' LTR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    1..454
                     /locus_tag="U3"
                     /label=U3
                     /note="U3"
     misc_feature    455..551
                     /locus_tag="R"
                     /label=R
                     /note="R"
     misc_feature    552..636
                     /locus_tag="U5(1)"
                     /label=U5
                     /label=U5(1)
                     /note="U5"
     misc_feature    697..806
                     /locus_tag="HIV-PSI"
                     /label=HIV-PSI
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             790..1128
                     /locus_tag="HIV-1 gag (fragment)"
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=22.6"
                     /note="/other=CDS"
     misc_feature    1303..1536
                     /locus_tag="RRE"
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             1913..2013
                     /locus_tag="Rev (fragment)"
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
     misc_feature    2058..2175
                     /locus_tag="cPPT/CTS"
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    2233..2238
                     /locus_tag="SpeI"
                     /label=SpeI
     enhancer        2295..2598
                     /locus_tag="CMV enhancer"
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        2599..2802
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        2804..2823
                     /locus_tag="TetO-1"
                     /label=TetO-1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     promoter        2825..2844
                     /locus_tag="TetO-2"
                     /label=TetO-2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     misc_feature    2949..2956
                     /locus_tag="NotI"
                     /label=NotI
     misc_feature    2952..2961
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     misc_feature    2958..2960
                     /locus_tag="Initiator Met"
                     /label=Initiator Met
     misc_feature    2961..2966
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    2973..2978
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    2991..2996
                     /locus_tag="AgeI"
                     /label=AgeI
     misc_feature    3003..3008
                     /locus_tag="XbaI"
                     /label=XbaI
     misc_feature    3021..3041
                     /locus_tag="TEV protease cleavage site"
                     /label=TEV protease cleavage site
     misc_feature    3054..3341
                     /locus_tag="SumoEu1"
                     /label=SumoEu1
                     /note="SumoEu1 Vera et al. 2019"
     misc_feature    3339..3341
                     /locus_tag="SUMO protease cleavage site"
                     /label=SUMO protease cleavage site
     misc_feature    3408..3452
                     /locus_tag="ALFA tag"
                     /label=ALFA tag
                     /note="ALFA tag"
     misc_feature    3459..3526
                     /locus_tag="P2A"
                     /label=P2A
                     /note="P2A"
     misc_feature    3552..4250
                     /locus_tag="TagBFP"
                     /label=TagBFP
                     /note="TagBFP"
     misc_feature    4254..4259
                     /locus_tag="XhoI"
                     /label=XhoI
     misc_feature    4261..4849
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    4649
                     /locus_tag="exchange"
                     /label=exchange
     misc_feature    4902..4921
                     /locus_tag="cPPT/CTS (fragment)"
                     /label=cPPT/CTS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.9"
                     /note="/other=misc_feature"
     LTR             4923..5177
                     /locus_tag="3' LTR (truncated)"
                     /label=3' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    4923..4997
                     /locus_tag="dU3 fragment, deletion of part of U3"
                     /label=dU3 fragment, deletion of part of U3
                     /note="dU3 fragment, deletion of part of U3"
     misc_feature    4998..5094
                     /locus_tag="R(1)"
                     /label=R
                     /label=R(1)
                     /note="R"
     misc_feature    5095..5177
                     /locus_tag="U5"
                     /label=U5
                     /note="U5"
     promoter        5310..5414
                     /locus_tag="AmpR promoter"
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5415..6275
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      6446..7034
                     /locus_tag="ori"
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             7217..7271
                     /locus_tag="lacI (fragment)"
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     rep_origin      7277..7370
                     /locus_tag="SV40 ori (fragment)"
                     /label=SV40 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=69.1"
                     /note="/other=rep_origin"
     CDS             7395..7432
                     /locus_tag="lacI (fragment)(1)"
                     /label=lacI (fragment)
                     /label=lacI (fragment)(1)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.5"
                     /note="/other=CDS"
     protein_bind    7445..7466
                     /locus_tag="CAP binding site"
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        7481..7511
                     /locus_tag="lac promoter"
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    7519..7535
                     /locus_tag="lac operator"
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    7569..7652
                     /locus_tag="SV40 poly(A) signal (fragment)"
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=62.2"
                     /note="/other=polyA_signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gacgcgttga
     2221 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2281 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2341 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2401 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2461 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2521 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2581 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     2641 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     2701 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     2761 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga
     2821 gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     2941 cggctagagc ggccgccatg ggatccggtt ctgctagcgg ttcaggttct accggtggat
     3001 catctagaac tagcggtagc gagaatttgt attttcaggg tggtagcgga tcttctgctg
     3061 cgggtggcga agaagataag aaaccggcag gtggcgaagg tggcggtgcc catatcaacc
     3121 tgaaagtgaa aggtcaagac ggcaacgaag tctttttccg catcaaacgt tctacccagc
     3181 tgaaaaagct gatgaacgca tactgtgacc gtcagtctgt agacatgaag gcaattgctt
     3241 tcctctttaa gggtcgtcgc ctacgtgcgg aaaggacccc ggatgaactg gaaatggaag
     3301 atggcgacga aatcgacgca atgctgcatc agactggtgg cggatctgga agtggatctg
     3361 gatcaggaag tggttctgga tcaggtagtg gttctggtag tggatcacca tctcgcctgg
     3421 aagaggaact gcgtcgtcgc ctgaccgaac caaccggagg aagcggagct actaacttca
     3481 gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctggaacg ggcggagctg
     3541 gttcaacgtc tatgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg
     3601 gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
     3661 gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
     3721 tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc
     3781 ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg
     3841 aagacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct
     3901 acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa
     3961 cactcggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca
     4021 gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca
     4081 catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact
     4141 acagactgga aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg
     4201 cagtggccag atactgcgac ctccctagca aactggggca caagcttaat taactcgagt
     4261 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4321 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4381 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4441 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4501 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4561 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4621 ttgggcactg acaattccgt ggtgttgttg gggaaatcat cgtcctttcc ttggctgctc
     4681 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4741 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4801 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggtaccttt
     4861 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4921 actggaaggg ctaattcact cccaacgaag acaagatcac ctgcaggaca ggcgcgccct
     4981 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5041 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5101 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5161 gtggaaaatc tctagcaccc gggcgattaa ggaaagggct agatcattct tgaagacgaa
     5221 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5281 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5341 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5401 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5461 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5521 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5581 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5641 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5701 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5761 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5821 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5881 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5941 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6001 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6061 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6121 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6181 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6241 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6301 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6361 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7141 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7201 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7261 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7321 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7381 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7441 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7501 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     7561 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7621 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     7681 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
     7741 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
     7801 gtactacaaa cttagtagt
//
