LOCUS       Exported                5074 bp ds-DNA     circular SYN 06-JUL-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Cytosolic GC7F-gpd-2-mScarlet-I in DSP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5074)
  AUTHORS   Dhaval
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 2, 2023 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5074
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     53..69
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     exon            182..544
                     /label=Exon 1
     misc_feature    335..337
                     /label=A52
     misc_feature    357..359
                     /label=L59
     misc_feature    360..362
                     /label=E60
     intron          545..595
                     /label=Intron 1
     exon            596..1006
                     /label=Exon 2
     intron          1007..1057
                     /label=Intron 2
     exon            1058..1279
                     /label=Exon 3
     misc_feature    1187..1189
                     /label=L302
     intron          1280..1330
                     /label=Intron 3 (Syntron)
     exon            1331..1685
                     /label=Exon 4
     misc_feature    1454..1456
                     /label=M374
     misc_feature    1466..1468
                     /label=M378
     misc_feature    1469..1471
                     /label=K379
     misc_feature    1482..1483
                     /label=T383
     misc_feature    1568..1570
                     /label=T412
     sig_peptide     1685
                     /label=egl-13 NLS
     misc_signal     1711..1932
                     /label=gpd-2/3 operon linker sequence
                     /note="ApEinfo_fwdcolor=#b11fff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     exon            1933..2103
                     /label=mScarlet Exon 1
     sig_peptide     1933..1971
                     /label=Shared Start
     intron          2104..2190
                     /label=Intron 1 with PATCs
     exon            2191..2412
                     /label=mScarlet Exon 2
     intron          2413..2465
                     /label=Intron 2 with PATCs
     exon            2466..2636
                     /label=mScarlet Exon 3
     intron          2637..2687
                     /label=Intron 3 (Syntron)
     exon            2688..2846
                     /label=mScarlet Exon 4 NO PEST
     CDS             2847..2849
                     /codon_start=1
                     /label=GCaMP7s
                     /translation=""
     terminator      2850..3173
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(3224..3240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(3302..3890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4061..4876)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(4877..4979)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagtaaaacg
       61 acggccagtt tatctagtca gcttgattct agctgatcgt ggaccggaag gtgagccagt
      121 gagttgattg cagtccagtt acgctggagt ctgaggctcg tcctgaatga tatgcggccg
      181 catgggatcc caccaccacc accaccacgg aatggcctct atgaccggag gacaacaaat
      241 gggacgcgac ctttacgatg acgacgacaa ggaccttgct accatggttg attcttctcg
      301 ccgcaagtgg aacaagactg gacatgctgt ccgtgccatc ggacgtcttt cctcccttga
      361 gaacgtttac atcaaggctg ataagcaaaa gaacggaatt aaggctaact tcaagatccg
      421 tcacaacatc gaagatggag gagtccaact tgcttaccat taccaacaaa acactccaat
      481 cggagatgga ccagttcttc ttccagataa ccactacctt tccgttcaat ccaagctctc
      541 taaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcaggatcc
      601 aaacgagaag cgtgaccaca tggtccttct tgagttcgtc accgctgctg gaatcaccct
      661 tggaatggat gagctttata agggaggaac tggaggatct atggtttcta agggagagga
      721 gcttttcact ggagttgtcc caatccttgt cgagcttgat ggagatgtta acggacacaa
      781 gttctctgtt tctggagagg gagagggaga cgctacctac ggaaagctta ctcttaagtt
      841 catctgcact actggaaagc ttccagttcc atggccaacc cttgttacca cccttactta
      901 cggagttcaa tgcttctctc gttacccaga tcatatgaag caacatgatt tcttcaagtc
      961 tgctatgcca gagggataca tccaagagcg tactatcttc ttcaaggtaa gtttaaacag
     1021 ttcggtacta actaaccata catatttaaa ttttcaggat gatggaaact acaagacccg
     1081 tgctgaggtt aagttcgagg gagataccct tgttaaccgt atcgagctta agggaatcga
     1141 tttcaaggaa gatggaaaca tccttggaca taagcttgag tacaaccttc cagatcaact
     1201 taccgaggaa caaatcgctg agttcaagga gcttttctcc cttttcgata aggatggaga
     1261 cggaactatc actaccaagg taagttttct agagaccttt actaactaac taggtctcat
     1321 agattttcag gagcttggaa ccgttatgcg ttcccttgga caaaacccaa ctgaggctga
     1381 gcttcaagat atgatcaacg aggttgacgc tgatggagat ggaactatcg atttcccaga
     1441 gttccttact atgatggctc gcaagatgaa gtacactgat tctgaggagg agatccgcga
     1501 ggctttccgc gtcttcgaca aggacggaaa cggatacatt tccgctgctg agctccgtca
     1561 cgttatgact aacctcggag agaagcttac cgacgaggag gtcgacgaga tgatccgtga
     1621 ggctgatatc gacggagacg gacaagttaa ctacgaggag ttcgttcaaa tgatgactgc
     1681 caagtaagct gtctcatcct actttcacct agttaactgc ttgtcttaaa atctatgctt
     1741 ctctttagta tctaaaattt tcctagaagc ttacaagtat ataaatggtc tcttctcaat
     1801 aaaggttgta tatttattca tcttattgaa tctgccattt cctcgttttt gcgagtttat
     1861 ataccttcca attttctttc tattgtattt tcaacttcta attttaattc agggaaactg
     1921 cttcaacgca tcatggtttc caagggagag gctgttatca aggaattcat gacttacgaa
     1981 cttatcaagg agtttatgcg ctttaaggtt cacatggaag gatctatgaa cggacacgaa
     2041 ttcgaaatcg aaggagaagg agaaggacgt ccatacgagg gaactcaaac tgctaagctt
     2101 aaggtaagtt tatctaaaaa gtttttcatt caaaatgtgt aaaaattcat ttaaaataac
     2161 ccaaaaatca ttaatcctcg atattttcag gttactaaag gaggaccact tccattctct
     2221 tgggatatcc tttctccaca attcatgtac ggatctcgcg ctttcatcaa gcacccagct
     2281 gatatcccag attactacaa gcaatctttc ccagaaggat tcaaatggga gcgtgttatg
     2341 aacttcgaag atggaggagc tgttaccgtt acccaagata cctcccttga ggatggaacc
     2401 cttatctaca aggtattttc ctgcattttt caactgggaa aatgaaagaa aatcgataat
     2461 ttcaggttaa gcttcgtgga actaatttcc caccagatgg accagttatg caaaagaaga
     2521 ctatgggatg ggaggcttct accgagcgcc tttacccaga ggatggagtc cttaagggag
     2581 atatcaagat ggctcttcgt cttaaggatg gaggacgtta ccttgctgat ttcaaggtaa
     2641 gttttctaga gacctttact aactaactag gtctcataga ttttcagact acttacaagg
     2701 ctaagaagcc agttcaaatg ccaggagctt acaacgttga tcgtaagctt gatatcacct
     2761 ctcacaacga ggattacacc gttgttgagc aatacgagcg ttctgaggga cgtcactcta
     2821 ccggaggaat ggatgagctt tacaagtaaa tgcaaaatcc tttcaagcat tcccttcttc
     2881 tctatcactc ttctttcttt ttgtcaaaaa attctctcgc taatttattt gcttttttaa
     2941 tgttattatt ttatgacttt ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg
     3001 ctatcaaaat gtgattctgt ctgatgtact ttcacaatct ctcttcaatt ccattttgaa
     3061 gtgctttaaa cccgaaaggt tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg
     3121 ttgagtgacc caacaaaaag aggaaacttt attgtgccgc caagaaaaaa gtctcatacg
     3181 tatgatcggt ccacgatcag ctagattatc tagtcagctt gatgtcatag ctgtttcctg
     3241 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3301 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3361 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3421 gctctcctgt tccgaccctg tcgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3481 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3541 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3601 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3661 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3721 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3781 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3841 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3901 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3961 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4021 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttagaaaaac tcatcgagca
     4081 tcaagtgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     4141 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     4201 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     4261 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     4321 agagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     4381 aatcactcgc accaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     4441 cgcgatcgcc gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     4501 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     4561 ctgttttccc tgggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     4621 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagcctgacc atctcatctg
     4681 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     4741 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     4801 acccatataa atcagcatcc atgttggaat ttaatcgcgg cttcaagcaa gacgtttccc
     4861 gttgaatatg gctcatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     4921 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcac
     4981 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     5041 tataaaaata ggcgtatcac gaggcccttt cgtc
//
