LOCUS       Exported                5140 bp ds-DNA     circular SYN 11-MAR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    GC7s-CAAX-gpd-2-mScarlet-I-CAAX in DSP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Dhaval
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 2, 2023 from SnapGene 3.3.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     53..69
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     exon            182..544
                     /label=Exon 1
     misc_feature    357..359
                     /label=L59
     misc_feature    360..362
                     /label=E60
     misc_feature    413..415
                     /label=H78
     intron          545..595
                     /label=Intron 1
     exon            596..1006
                     /label=Exon 2
     intron          1007..1057
                     /label=Intron 2
     exon            1058..1279
                     /label=Exon 3
     misc_feature    1187..1189
                     /label=L302
     intron          1280..1330
                     /label=Intron 3 (Syntron)
     exon            1331..1684
                     /label=Exon 4
     misc_feature    1454..1456
                     /label=M374
     misc_feature    1466..1468
                     /label=M378
     misc_feature    1469..1471
                     /label=K379
     misc_feature    1475..1477
                     /label=R381
     misc_feature    1481..1483
                     /label=T383
     misc_feature    1508..1510
                     /label=G392
     misc_feature    1568..1570
                     /label=T412
     sig_peptide     1685..1720
                     /label=ras-2 CAAX
     misc_signal     1721..1965
                     /label=gpd-2/3 operon linker sequence
                     /note="ApEinfo_fwdcolor=#b11fff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     exon            1966..2136
                     /label=mScarlet Exon 1
     sig_peptide     1966..2004
                     /label=Shared Start
     intron          2137..2223
                     /label=Intron 1 with PATCs
     exon            2224..2445
                     /label=mScarlet Exon 2
     intron          2446..2498
                     /label=Intron 2 with PATCs
     exon            2499..2669
                     /label=mScarlet Exon 3
     intron          2670..2720
                     /label=Intron 3 (Syntron)
     exon            2721..2879
                     /label=mScarlet Exon 4 NO PEST
     sig_peptide     2880..2915
                     /label=ras-2 CAAX
     terminator      2916..3239
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(3290..3306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(3368..3956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4127..4942)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(4943..5045)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagtaaaacg
       61 acggccagtt tatctagtca gcttgattct agctgatcgt ggaccggaag gtgagccagt
      121 gagttgattg cagtccagtt acgctggagt ctgaggctcg tcctgaatga tatgcggccg
      181 catgggatcc caccaccacc accaccacgg aatggcctct atgaccggag gacaacaaat
      241 gggacgcgac ctttacgatg acgacgacaa ggaccttgct accatggttg attcttctcg
      301 ccgcaagtgg aacaagactg gacatgctgt ccgtgtcatc ggacgtcttt cctcccttga
      361 gaacgtttac atcaaggctg ataagcaaaa gaacggaatt aaggctaact tccatatccg
      421 tcacaacatc gaagatggag gagtccaact tgcttaccat taccaacaaa acactccaat
      481 cggagatgga ccagttcttc ttccagataa ccactacctt tccgttcaat ccaagctctc
      541 taaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt ttcaggatcc
      601 aaacgagaag cgtgaccaca tggtccttct tgagttcgtc accgctgctg gaatcaccct
      661 tggaatggat gagctttata agggaggaac tggaggatct atggtttcta agggagagga
      721 gcttttcact ggagttgtcc caatccttgt cgagcttgat ggagatgtta acggacacaa
      781 gttctctgtt tctggagagg gagagggaga cgctacctac ggaaagctta ctcttaagtt
      841 catctgcact actggaaagc ttccagttcc atggccaacc cttgttacca cccttactta
      901 cggagttcaa tgcttctctc gttacccaga tcatatgaag caacatgatt tcttcaagtc
      961 tgctatgcca gagggataca tccaagagcg tactatcttc ttcaaggtaa gtttaaacag
     1021 ttcggtacta actaaccata catatttaaa ttttcaggat gatggaaact acaagacccg
     1081 tgctgaggtt aagttcgagg gagataccct tgttaaccgt atcgagctta agggaatcga
     1141 tttcaaggaa gatggaaaca tccttggaca taagcttgag tacaaccttc cagatcaact
     1201 taccgaggaa caaatcgctg agttcaagga gcttttctcc cttttcgata aggatggaga
     1261 cggaactatc actaccaagg taagttttct agagaccttt actaactaac taggtctcat
     1321 agattttcag gagcttggaa ccgttatgcg ttcccttgga caaaacccaa ctgaggctga
     1381 gcttcaagat atgatcaacg aggttgacgc tgatggagat ggaactatcg atttcccaga
     1441 gttccttact atgatggctc gcaagatgaa gtaccgcgat actgaggagg agatccgcga
     1501 ggctttcgga gtcttcgaca aggacggaaa cggatacatt tccgctgctg agctccgtca
     1561 cgttatgact aacctcggag agaagcttac cgacgaggag gtcgacgaga tgatccgtga
     1621 ggctgatatc gacggagacg gacaagttaa ctacgaggag ttcgttcaaa tgatgactgc
     1681 caagaaacga aaggataagg gaaaatgttt aatttcctaa gctgtctcat cctactttca
     1741 cctagttaac tgcttgtctt aaaatctatg cttctcttta gtatctaaaa ttttcctaga
     1801 agcttacaag tatataaatg gtctcttctc aataaaggtt gtatatttat tcatcttatt
     1861 gaatctgcca tttcctcgtt tttgcgagtt tatatacctt ccaattttct ttctattgta
     1921 ttttcaactt ctaattttaa ttcagggaaa ctgcttcaac gcatcatggt ttccaaggga
     1981 gaggctgtta tcaaggaatt catgacttac gaacttatca aggagtttat gcgctttaag
     2041 gttcacatgg aaggatctat gaacggacac gaattcgaaa tcgaaggaga aggagaagga
     2101 cgtccatacg agggaactca aactgctaag cttaaggtaa gtttatctaa aaagtttttc
     2161 attcaaaatg tgtaaaaatt catttaaaat aacccaaaaa tcattaatcc tcgatatttt
     2221 caggttacta aaggaggacc acttccattc tcttgggata tcctttctcc acaattcatg
     2281 tacggatctc gcgctttcat caagcaccca gctgatatcc cagattacta caagcaatct
     2341 ttcccagaag gattcaaatg ggagcgtgtt atgaacttcg aagatggagg agctgttacc
     2401 gttacccaag atacctccct tgaggatgga acccttatct acaaggtatt ttcctgcatt
     2461 tttcaactgg gaaaatgaaa gaaaatcgat aatttcaggt taagcttcgt ggaactaatt
     2521 tcccaccaga tggaccagtt atgcaaaaga agactatggg atgggaggct tctaccgagc
     2581 gcctttaccc agaggatgga gtccttaagg gagatatcaa gatggctctt cgtcttaagg
     2641 atggaggacg ttaccttgct gatttcaagg taagttttct agagaccttt actaactaac
     2701 taggtctcat agattttcag actacttaca aggctaagaa gccagttcaa atgccaggag
     2761 cttacaacgt tgatcgtaag cttgatatca cctctcacaa cgaggattac accgttgttg
     2821 agcaatacga gcgttctgag ggacgtcact ctaccggagg aatggatgag ctttacaaga
     2881 aacgaaagga taagggaaaa tgtttaattt cctaaatgca aaatcctttc aagcattccc
     2941 ttcttctcta tcactcttct ttctttttgt caaaaaattc tctcgctaat ttatttgctt
     3001 ttttaatgtt attattttat gactttttat agtcactgaa aagtttgcat ctgagtgaag
     3061 tgaatgctat caaaatgtga ttctgtctga tgtactttca caatctctct tcaattccat
     3121 tttgaagtgc tttaaacccg aaaggttgag aaaaatgcga gcgctcaaat atttgtattg
     3181 tgttcgttga gtgacccaac aaaaagagga aactttattg tgccgccaag aaaaaagtct
     3241 catacgtatg atcggtccac gatcagctag attatctagt cagcttgatg tcatagctgt
     3301 ttcctgacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3481 tcgtgcgctc tcctgttccg accctgtcgc ttaccggata cctgtccgcc tttctccctt
     3541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3781 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4081 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttag aaaaactcat
     4141 cgagcatcaa gtgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     4201 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     4261 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     4321 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     4381 atggcaagag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     4441 catcaaaatc actcgcacca accaaaccgt tattcattcg tgattgcgcc tgagcgagac
     4501 gaaatacgcg atcgccgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     4561 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     4621 ggaatgctgt tttccctggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     4681 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagc ctgaccatct
     4741 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     4801 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     4861 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcttc aagcaagacg
     4921 tttcccgttg aatatggctc attttagctt ccttagctcc tgaaaatctc gataactcaa
     4981 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     5041 gatcacacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     5101 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
