LOCUS       pTol2_(-3kb)nrg1       10515 bp DNA     circular SYN 03-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 3, 2023 from SnapGene Viewer 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10515
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     gene            337..622
                     /label=medaka tyrosinase genomic (from AB010101)
                     /note="/ApEinfo_fwdcolor=#006600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#006600"
     LTR             complement(622..638)
                     /label=Tol2 3' TIR
                     /note="/ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff0000"
     misc_structure  complement(811..1157)
                     /label=Tol2 exon4 136-482
                     /note="/ApEinfo_fwdcolor=#aa0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#aa0000"
     primer_bind     1169..1190
                     /label=M13R
                     /note="/ApEinfo_fwdcolor=#777777
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#777777"
     protein_bind    1205..1225
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    1205..1211
                     /label=att4_shared
                     /note="/ApEinfo_fwdcolor=#4a33cc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#4a33cc"
     misc_recomb     1205..1211
                     /label=attR4
                     /note="/ApEinfo_fwdcolor=#5d40ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#5d40ff"
     misc_feature    1228..1248
                     /label=MCS
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
     primer_bind     complement(1228..1248)
                     /label=P4_P1R_R1
     primer_bind     1232..1268
                     /label=T2_3055_G_F1
     primer_bind     1232..1249
                     /label=T2_N_Gbloc_G_F
     misc_feature    1249..4312
                     /label=T2_3055_cons
     misc_feature    1249..1268
                     /label=3055
     primer_bind     1249..1268
                     /label=T2_3055_F
     primer_bind     1471..1494
                     /label=T2_N_Gbloc_G_F
     primer_bind     1671..1686
                     /label=T2_2014_F
     primer_bind     1722..1736
                     /label=T2_2524_F
     primer_bind     2182..2202
                     /label=T2_2067_F
     misc_feature    2235..2253
                     /label=2014
     primer_bind     2235..2253
                     /label=T2_2014_F
     misc_feature    2266..2286
                     /label=1983
     primer_bind     2266..2286
                     /label=T2_1983_F
     primer_bind     2537..2558
                     /label=T2_1707_F
     primer_bind     complement(2865..2885)
                     /label=1677_R
     primer_bind     2865..2885
                     /label=1399_F
     misc_feature    3100..3124
                     /label=minus_F
     primer_bind     3100..3124
                     /label=T2_7kb_F6_60C
     misc_feature    3100..3121
                     /label=minus_F_57
     primer_bind     3100..3121
                     /label=T2_7_F6
     primer_bind     3100..3121
                     /label=T2_7kb_F5
     primer_bind     3558..3573
                     /label=T2_700bp_F1
     primer_bind     3935..3956
                     /label=T2p_F1
     primer_bind     4068..4087
                     /label=T2p_F3
     primer_bind     4101..4122
                     /label=T2p_F2
     primer_bind     4106..4125
                     /label=T2p_F7
     primer_bind     4127..4142
                     /label=T2_1kb_R7_to_ATG_SNP_C
     primer_bind     4151..4168
                     /label=TII-R
     primer_bind     4202..4222
                     /label=TII-R2
     primer_bind     complement(4245..4268)
                     /label=T2_1kb_R6
     primer_bind     complement(4248..4276)
                     /label=T2_1kb_R5
     primer_bind     complement(4298..4349)
                     /label=T2_1kb_R7_to_ATG_SNP_A
     primer_bind     complement(4298..4349)
                     /label=T2_1kb_R7_to_ATG_SNP_C
     primer_bind     complement(4298..4325)
                     /label=T2_R9_xho
     primer_bind     complement(4298..4312)
                     /label=T2_1_R8
     primer_bind     complement(4301..4329)
                     /label=T2_G_R8
     primer_bind     complement(4301..4325)
                     /label=T2_R8_xho
     misc_feature    4313..4335
                     /label=MCS
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
     primer_bind     4313..4331
                     /label=P4_P1R_F1
     primer_bind     4314..4328
                     /label=T2_7kb_F5
     primer_bind     4314..4328
                     /label=T2_7kb_F6_60C
     primer_bind     complement(4334..4349)
                     /label=T2_1kb_R6
     primer_bind     complement(4335..4349)
                     /label=T2_1kb_R5
     protein_bind    4336..4360
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     4337..4380
                     /label=attB1_GFP_F
     misc_feature    4337..4343
                     /label=attR1
                     /note="/ApEinfo_fwdcolor=#ffff80
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff0080"
     misc_feature    4344..4360
                     /label=attL1
                     /note="/ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green"
     CDS             4363..5168
                     /codon_start=1
                     /label=EGFPCAAX insert
                     /note="/ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKGGGRSKLNPPDESGPGCMSCKCVLS*NPA"
     CDS             4363..5079
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     4437..4455
                     /label=GFP_Nterm_F
     primer_bind     complement(4437..4455)
                     /label=GFP_Nterm_R
     primer_bind     complement(4545..4564)
                     /label=1167
     primer_bind     complement(4602..4620)
                     /label=GFP_Nterm_R2
     primer_bind     complement(4743..4761)
                     /label=eGFP_R3
     primer_bind     4816..4835
                     /label=GFP_F
                     /note="julie"
     primer_bind     4829..4848
                     /label=FoxD3-GFP_F2
                     /note="Julie - mismatches presenther seq: https:    
                     ACAAGCAAAAGAACGGCATC"
     primer_bind     4905..4923
                     /label=GFP_Cterm_F
     primer_bind     complement(4915..4934)
                     /label=FoxD3_GFP_R2
                     /note="Julie - 5 mismatchesher seq:https:    
                     TCGCCAATTGGAGTATTTTG"
     primer_bind     complement(4958..4977)
                     /label=GFP_R
                     /note="julie"
     primer_bind     complement(5137..5182)
                     /label=attB2_CAAX_R
     protein_bind    complement(5159..5183)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5159..5168
                     /label=attL2
                     /note="/ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green"
     attenuator      5169..5182
                     /label=attR2
     misc_feature    5185..5605
                     /label=afp 3'UTR
     protein_bind    complement(5607..5627)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_recomb     5614..5627
                     /label=attR3
                     /note="/ApEinfo_fwdcolor=#5370ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#5370ff"
     misc_feature    5614..5621
                     /label=att3_shared
                     /note="/ApEinfo_fwdcolor=#4259cc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#4259cc"
     primer_bind     complement(5636..5651)
                     /label=M13F
                     /note="/ApEinfo_fwdcolor=#777777
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#777777"
     polyA_signal    5667..5864
                     /label=SV40 late polyA (from pCS2+)
                     /note="/ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ffff7f"
     polyA_signal    complement(5923..6055)
                     /label=SV40 late polyA (from pCS2+)(1)
                     /note="/ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=SV40 late polyA (from pCS2+)
                     /ApEinfo_revcolor=#ffff7f"
     primer_bind     6077..6092
                     /label=T2_R8_xho
     primer_bind     6077..6092
                     /label=T2_R9_xho
     CDS             complement(6106..6822)
                     /label=EGFP
                     /note="/ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#00ff00"
     primer_bind     6208..6227
                     /label=GFP_R
                     /note="julie"
     primer_bind     6251..6270
                     /label=FoxD3_GFP_R2
                     /note="Julie - 5 mismatchesher seq:https:    
                     TCGCCAATTGGAGTATTTTG"
     primer_bind     complement(6262..6280)
                     /label=GFP_Cterm_F
     primer_bind     complement(6337..6356)
                     /label=FoxD3-GFP_F2
                     /note="Julie - mismatches presenther seq: https:    
                     ACAAGCAAAAGAACGGCATC"
     primer_bind     complement(6350..6369)
                     /label=GFP_F
                     /note="julie"
     primer_bind     6424..6442
                     /label=eGFP_R3
     primer_bind     6565..6583
                     /label=GFP_Nterm_R2
     misc_structure  complement(6618..6631)
                     /label=EGFP-like chromophore
                     /note="/ApEinfo_fwdcolor=#00cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#00cc00"
     primer_bind     6621..6640
                     /label=1167
     primer_bind     complement(6730..6748)
                     /label=GFP_Nterm_F
     primer_bind     6730..6748
                     /label=GFP_Nterm_R
     primer_bind     complement(6805..6822)
                     /label=attB1_GFP_F
     promoter        complement(6823..7721)
                     /label=cmlc2 promoter
                     /note="from zebrafish - Blat search of danRer7"
     misc_feature    6828..7068
                     /label=minimal cmlc2
                     /note="/ApEinfo_fwdcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=green"
     primer_bind     complement(6981..7002)
                     /label=1177
     misc_structure  complement(7728..8062)
                     /label=Tol2 exon1 bp1-335 of 461
                     /note="/ApEinfo_fwdcolor=#aa0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#aa0000"
     LTR             complement(8226..8244)
                     /label=Tol2 5' TIR
                     /note="/ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff0000"
     misc_structure  complement(8245..8281)
                     /label=medaka tyrosinase ex5 frag
                     /note="/ApEinfo_fwdcolor=#006600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#006600"
     promoter        complement(8330..8349)
                     /label=T3
                     /note="/ApEinfo_fwdcolor=#777777
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#777777"
     primer_bind     complement(8365..8386)
                     /label=M13R(1)
                     /note="/ApEinfo_fwdcolor=#777777
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_label=M13R
                     /ApEinfo_revcolor=#777777"
     rep_origin      complement(8713..9386)
                     /direction=LEFT
                     /label=pUC ori
                     /note="/ApEinfo_fwdcolor=#999999
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#999999"
     CDS             complement(9534..10391)
                     /label=ampR
                     /note="/ApEinfo_fwdcolor=#ff7f00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff7f00"
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccgggctg gctaagaact catcagcctc
      361 cccggtccat ctacccacgt accaatgcac caattggcca caatgacggc tactacatgg
      421 tgccattcct tcctctttat aggaatggag actacctcct gtccaacaag gctcttggat
      481 acgagtacgc ctacctgttg gacccaggtc attgcacaac accagaaatg ccctctgatc
      541 tgcaaaagac gtgaatatct gttcagacac ccatatccac tctgttccac acaggtcaga
      601 ggtttgtcca ggagttcttg acagaggtgt aaaaagtact caaaaatttt actcaagtga
      661 aagtacaagt acttagggaa aattttactc aattaaaagt aaaagtatct ggctagaatc
      721 ttacttgagt aaaagtaaaa aagtactcca ttaaaattgt acttgagtat taaggaagta
      781 aaagtaaaag caagaaagaa aactagagat tcttgtttaa gcttttaatc tcaaaaaaca
      841 ttaaatgaaa tgcatacaag gttttatcct gctttagaac tgtttgtatt taattatcaa
      901 actataagac agacaatcta atgccagtac acgctactca aagttgtaaa acctcagatt
      961 taacttcagt agaagctgat tctcaaaatt gttagtgtca agcctagctc ttttggggct
     1021 gaaaagcaat cctgcagtgc tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt
     1081 attagtcttg atagagaggc tgcaaatagc aggaaacgtg agcagagact ccctggtgtc
     1141 tgaaacacag gccagatggg ccctcgagca ggaaacagct atgaccatga ttacgccaag
     1201 ctatcaactt tgtatagaaa agttgctgga tcccatcgat tcgaattccc actgagccac
     1261 catgtagcct atgttttatg ttcaatccac ttaaatttgt aaatatgtgt aagttaactg
     1321 atttgtgttg ggacaacagg aaggaattat gtggaaccca acatgtttta acagttgttc
     1381 aatttacatg tagaaattag ttccaagacc aatgcttgaa cacaactagg ttttagaaac
     1441 tgacccaatt aaaaaaggac aactttgttt caattgtgct tgtgttaaag ctgccttaca
     1501 aagatgtaca gggtaataat taaagagaaa gttctttatt gtggaatatc ttcaatataa
     1561 acggtatcaa gccaaaatgt gggggggccc tggtgaagtc agttgttagc actgttgcta
     1621 ccaagccagt cagtttctgc gccaagttta cacgttctct ctgtgctcaa gtgggtttcc
     1681 cccaggttcc ccagtttcct cctaccatcc aaaaacatac accagagaca tgctcctagt
     1741 aagtagttat ctcttaagag cattcactat ctgttcatta tctactacag caggggagtt
     1801 ctcgagattt acctgagccc aaactctcct ttcgctttgc aaatgggaga gagccccgga
     1861 ctcgaggatc ttatgagctc agggctctct cccgggaccg catgccaaac aagcttgaag
     1921 taagaatgat tctaagggcc tacgccgttt tagggccccc agagatagga tgaggggccc
     1981 atcacaaccc ccccccctct tcatttttat ccgcaacacc catgaaatgt tttcttaggg
     2041 cctgtaccgg ccagcggcgc ccctgtctgg ataaaaaatg cttttctgac ttgatttttt
     2101 attttttttg tcttgtgttt gtctagttca aaaatggtag catggtgact cattggttcg
     2161 cactgttgcc taacagcaag aagatcgctg gtttaagtcc cggctggtcc gtttttgtgt
     2221 ggagattgca tggtcgtgtg ggtttcctcc aggtgcttcg atttccccca cattccacag
     2281 tcatgcacta taggtggatt gcatgaacta aactagctgt agtgtatgag agtgtgtgtg
     2341 aatgatagtg tgtatacgtg tttcctagta ctgggttgct gtggcaatcc cagattaaca
     2401 aaaggatgaa taattagcca ataatttatt agcttgaggg acatgatagc attgaaactg
     2461 aacatatatc gactgataac taatttagcc accaatatat tgtttataac cgccaaaagg
     2521 tttaacagaa gtgcctggat tgcagacatg gcaaacgctt tggacgttat ctcccagaac
     2581 actgatttgc tgtaactgca gcctaaatta agacatttcc agcacctctt taaaataact
     2641 gtccttttcc agagcacgtc ctgaattccc aagtaatggc ccttttagaa tcagttggtg
     2701 ttagcaactc tttcactcca tgctttaaat aatacacagt ctaaattggc acggacataa
     2761 aagtcacatt atgaaacaaa aagcagctta attttgcaga ttattatttg gtttcactag
     2821 gttaatacac cacacaagag ttctcaactc cggccccaga gacccccttt catgcagagt
     2881 tcagctccat aatcaaacac acctgaacca actaatccat gtgtcaatac acacactagc
     2941 agtcttagga gttttctcac taaattgaca ctttcactaa tatactgcta ttatttgagt
     3001 ctaaatgtat ccctgtattt attttcagga cctcacatgt gctaaacttg ggtaacactt
     3061 tttattttga tggtccactt gagtattagt agactgtctg cttagcaact gttgatactg
     3121 ctccttcaac agacatttaa ctgactataa gaaactttgc aagtacagtt gaagtcagaa
     3181 tatccccccc ccccctgttt attttccccc caatttctgt gaacggacag ctgatttttt
     3241 tcaacacatt tttaaatgtg aaaaaatatt attagaaata ctttgaaaat ttctgcattg
     3301 atcctgaggg ccagcctgaa cacccactag tgacctactt acacctgctg cttctccacg
     3361 atagacgtcc aatgttctcc agcctcctgt gcctagactg caggtctgca caagatgttt
     3421 ggccagagga gaaattgtcg tgcccaactg agtctggttt ctctcaaggt cttttccttc
     3481 acttttgtca attggtgaag ttatgtttct cgccactgtc cccactggct tgcttcctgt
     3541 tgaaaaaatc cagcttaaac cagcctaagc tggtttaagc tggatttttc agcagggttg
     3601 gtttgggact tgtggagctg cgcaatgatg gatttgttct tcattgtttg gactttcagc
     3661 agtgaaaaat taaaccacac tgaactgaat taaactgaac ttcaactcta aaaactggac
     3721 tgacggtttc aattcactag aacttctatg ttaggctgct ttgacccaat cattgtaaaa
     3781 gcgttaaaga aaaaaaaatt aaattgaaag ctaatatgtt cttgataaaa tgtccctaaa
     3841 tagacataaa catgtttaga gacagtttat ttaattttta ttattattta aatttaagta
     3901 atattatttg aaaaatgatc acttttatat cagaggcaat catctatatg ccgacgaaga
     3961 ttattgttga ctatttcata tttaaacagt ggttttcttt tttcatatta gcctactgaa
     4021 gtacacagta cttactcata tttagtagct atgttcacat tgaaaatccc gtcccactta
     4081 attgaccata agctggcaat aattagcgtg attccacagt ggaccgccac cccgcgctgt
     4141 ccttggtcgc ggtgtcggtg gggaaatcct gctcaccctc ggctgggatc tttctcaccc
     4201 cgcctcctgt aatacgatca gctgaagggc taacagaccc cacgcgcctc ttcattcact
     4261 ttttctgctc cactttaatt catccagcta aacgcaagga ccgagcgcgg aggagcctct
     4321 agaaccggtg gtaccgcaag tttgtacaaa aaagcaggct taatggtgag caagggcgag
     4381 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4441 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     4501 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     4561 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     4621 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     4681 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     4741 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     4801 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     4861 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     4921 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     4981 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5041 gccgccggga tcactctcgg catggacgag ctgtacaagg gaggaggaag atctaagctg
     5101 aaccctcctg atgagagtgg ccccggctgc atgagctgca agtgtgtgct ctcctgaaac
     5161 ccagctttct tgtacaaagt ggctattaag tctcagccac cgttaactga acatgtcaaa
     5221 acctgtggag actgttgaga tttgatgttc tgaaaagata aagcctataa ataaaatgtt
     5281 gcccaaattt cctgcctgat gtttttcttt gtctttgcta catggctttg ctgctcggat
     5341 cggctcactc tgtgtatgcc acgttcactt tgtactctcc ttctcacggt aggtttatta
     5401 tttttagatg tgcagttagt ttctgtgaaa taacacacca cacactgata ttgtctgtgc
     5461 attgacttgg tgagtgcaca ttgtttttga tcttgacata tttatatttg attgatcagg
     5521 tgaactgtgt gaatctaaag tgctccatac agatgttctg cattgaaaat attctcattt
     5581 tattagtgga agtgagtgta tgccagcaac tttattatac atagttgata attcactggc
     5641 cgtcgtttta cggtaccatc gatgatgatc cagacatgat aagatacatt gatgagtttg
     5701 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     5761 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     5821 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     5881 acaaatgtgg tatggctgat tatgatcctc tagatcagat ctcttgttta ttgcagctta
     5941 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6001 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctacgt
     6061 aatacgactc actatagttc tagaggctcg agaggggccg ctttacttgt acagctcgtc
     6121 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
     6181 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
     6241 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
     6301 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
     6361 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
     6421 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
     6481 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
     6541 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
     6601 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
     6661 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
     6721 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
     6781 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtcactg tctgctttgc
     6841 tgttggtctg ggctcctggg tcactggctt actaatggag tctttatgta tgaggactct
     6901 tatcaattgt tcttctataa aggtctgcag tgtttctgtt cgtcccctac atggacaccc
     6961 agagcctcct aaatacagga gccctgataa ctgcacaagt gctcagattc cagcagggtg
     7021 gaaaatgaga taaagtgtgc agatggggag ggggacgtga atgagagatt tgagggatga
     7081 aaaggatgga tgaacgcatt gaaaatagcc cctttcacaa aataatacca gtaaattgtc
     7141 atataattaa tgaccagacc tttacctgta aatgatgtga tgttcatata cagaagaatg
     7201 ccaacaattt acagatgatt ttacaaattc ttaattttcc ttttctaaat ggatttgttc
     7261 acacattatc tcttcaaagc aattgaattt tctggaaaag attggctgtg tgtgtaaaag
     7321 gtgctactga ggaatttaat gtgacatgga atgaagcaaa cagcagccta agacaggatg
     7381 gcaggaaaaa tgtcacatgt ttaaaataag agtgaaacca acgacctgga cttatagaga
     7441 ctgtgtgtcc ttaggcaaag ctcttgtaca aacagtgctt gtgactgtga aattcggggt
     7501 ttgcctggat tgtgttaaat gtgtgcttgg atgtatcata aaaaagaatc ggttctctgt
     7561 atttgacatt ttctgtaatt tatctaaagt tacaactggc tatgcctgat ttttatcact
     7621 attgaatcaa tcattttaaa gaatgttaag tctgctgatt tggagagctc atttacattt
     7681 attttgaatg tctcttattt aacacaactg atttaagctt tagatctgcg aagatacggc
     7741 cacgggtgct cttgatcctg tggctgattt tggactgtgc tgctcgcagc tgctgatgaa
     7801 tcacatactt cctccatttt cttccactga ttgactgtta taatttccct aatttccagg
     7861 tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg tcacttccaa aggaccaatg
     7921 aacatgtctg accaatttca tataatgtga aaacgatttt cataggcaga ataaataaca
     7981 tttaaattaa actgggcatc agcgcaattc aattggtttg gtaatagcaa gggaaaatag
     8041 aatgaagtga tctccaaaaa ataagtactt tttgactgta aataaaattg taaggagtaa
     8101 aaagtacttt tttttctaaa aaaatgtaat taagtaaaag taaaagtatt gatttttaat
     8161 tgtactcaag taaagtaaaa atccccaaaa ataatactta agtacagtaa tcaagtaaaa
     8221 ttactcaagt actttacacc tctggttctt gaccccctac cttcagcaag cccagcagat
     8281 ccactagttc tagagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga
     8341 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8401 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8461 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8521 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8581 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8641 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8701 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8761 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8821 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8881 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8941 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9001 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9061 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9121 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9181 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     9241 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9301 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9361 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9421 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9481 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9541 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9601 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9661 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9721 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9781 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9841 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9901 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9961 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10021 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10081 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10141 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10201 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10261 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10321 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10381 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10441 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10501 tttccccgaa aagtg
//
