LOCUS       Exported                4820 bp ds-DNA     circular SYN 05-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Baohui
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     489..513
                     /label=Validation primer 2-1728
     misc_feature    1451..2233
                     /label=CRISPR-Tag
     misc_feature    1451..1472
                     /label=TS1
     misc_feature    1473..1524
                     /label=52 bp distance
     misc_feature    1525..1546
                     /label=TS1
     misc_feature    1599..1620
                     /label=TS1
     misc_feature    1673..1694
                     /label=TS1
     misc_feature    1768..1789
                     /label=TS1
     misc_feature    1842..1863
                     /label=TS1
     misc_feature    1916..1937
                     /label=TS1
     misc_feature    1990..2011
                     /label=TS1
     misc_feature    2064..2085
                     /label=TS1
     misc_feature    2138..2159
                     /label=TS1
     misc_feature    2212..2233
                     /label=TS1
     primer_bind     complement(2369..2389)
                     /label=Validation primer AS2-1731
     rep_origin      complement(3068..3653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3824..4684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4685..4789)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattccatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacaga
      241 gaccgcggcc gcccctgccg ggacctggcg ctcgcctaag ccgcgtttgt actgtgtctt
      301 accatgcctg aaccggcaaa atccgctccg gcccctaaaa agggctccaa gaaagccgtc
      361 accaaagccc agaagaaaga cggcaagaag cgcaagcgca gccgcaaaga gagctactcc
      421 atctacgtgt acaaggtgct gaagcaggtc caccccgaca ccggcatctc gtccaaggcc
      481 atgggcatca tgaactcctt cgtcaacgac atcttcgagc gcatcgcggg agaggcttcc
      541 cgcctggcgc actacaacaa gcgctccacc atcacatccc gcgagatcca gacggccgtg
      601 cgcctgctgc tgcccggcga gctggccaag cacgccgtgt ccgagggcac caaggcggtc
      661 accaagtaca ccagctccaa gggatcgggt gggactagtg gcgtgagcaa gggcgaggag
      721 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
      781 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      841 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
      901 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
      961 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     1021 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     1081 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     1141 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     1201 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     1261 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     1321 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     1381 accggcggca tggacgagct gtacaagtga ggatccacgc gttgacaatc tgacaaaggt
     1441 ggcgtacctc gcaccgatgc tctccgagga ggcacctacg tacgcaccga tgctctccga
     1501 ggcagcaacg acatcggagt agtcgcaccg atgctctccg aggaggcgta cacggttgga
     1561 tcataggata gcaaaatctg acaaaggtgg cgtacctcgc accgatgctc tccgaggagg
     1621 cacctacgta cgcaccgatg ctctccgagg cagcaacgac atcggagtag tcgcaccgat
     1681 gctctccgag gaggcgtaca cggttggatc ataggatagg cagaactaca caccagggcg
     1741 aacaatctga caaaggtggc gtacctcgca ccgatgctct ccgaggaggc gtacacggtt
     1801 ggatcatagg atacagtaat ctgacaaagg tggcgtacct cgcaccgatg ctctccgagg
     1861 aggcacctac gtacgcaccg atgctctccg aggcagcaac gacatcggag tagtcgcacc
     1921 gatgctctcc gaggaggcgt acacggttgg atcataggat aacacaatct gacaaaggtg
     1981 gcgtacctcg caccgatgct ctccgaggag gcacctacgt acgcaccgat gctctccgag
     2041 gcagcaacga catcggagta gtcgcaccga tgctctccga ggaggcgtac acggttggat
     2101 cataggatat gtcaatctga caaaggtggc gtacctcgca ccgatgctct ccgaggaggc
     2161 acctacgtac gcaccgatgc tctccgaggc agcaacgaca tcggagtagt cgcaccgatg
     2221 ctctccgagg aggcgtacac ggttggatca taggataact cacatgtgag caaaaggccg
     2281 gaaccggtgc tggaagtggt tgagtacctg ccgggacctg gcgctcgctc gctcgagtcg
     2341 ccggctgctt gactccaaag gctcttttca gagccaccca cctaatcact agaaaagagc
     2401 ttgttcactt attcccttag tttcttttca taaagtaagt tattttagtg tgaaggtcat
     2461 gggaaatggc atacgtagct ttttaactat ttggaactcg aggtccccag tgcgtcattg
     2521 gatttgcttt tgaatctaga gcgtgtcttt actcattgtg ctgcttagcc ttcccaggag
     2581 tcggttctca attaggctgt tgggaatccg cctctttacc cgcccccact cccgccccac
     2641 acgcgccctg gtggctcctt gggtctgttt cattctaaaa cgaagtggct gagttcggct
     2701 gtcatttgcg agcgccaggt cccggcaggg cccgggcgac ggatggtgat ccccctggcc
     2761 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
     2821 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
     2881 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
     2941 ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg tcgacggtct
     3001 cgactcacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3061 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3121 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3181 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3241 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3301 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3361 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3421 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3481 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3541 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3601 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3661 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3721 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3781 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3841 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3901 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3961 cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4021 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4081 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4141 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4201 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4261 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4321 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4381 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4441 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4501 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4561 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4621 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4681 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4741 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4801 gaaaagtgcc acctgacgtc
//
