LOCUS       Exported                4784 bp ds-DNA     circular SYN 05-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Baohui
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          263..728
                     /organism="Homo sapiens"
                     /chromosome="7"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
     source          2144..2665
                     /organism="Homo sapiens"
                     /chromosome="7"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
     misc_feature    234..253
                     /label=sgTS2
     misc_feature    254..256
                     /label=PAM
     gene            263..728
                     /gene="HSPB1"
                     /gene_synonym="CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; 
                     HSP27; HSP28; SRP27"
                     /label=HSPB1 5'arm
                     /note="heat shock protein family B (small) member 1; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="GeneID:3315"
                     /db_xref="HGNC:HGNC:5246"
                     /db_xref="MIM:602195"
     CDS             join(360..423,542..728)
                     /codon_start=1
                     /gene="HSPB1"
                     /gene_synonym="CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; 
                     HSP27; HSP28; SRP27"
                     /product="heat shock protein beta-1"
                     /label=HSPB1
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS5583.1"
                     /db_xref="GeneID:3315"
                     /db_xref="HGNC:HGNC:5246"
                     /db_xref="MIM:602195"
                     /protein_id="NP_001531.1"
                     /translation="KHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEA
                     PMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK"
     misc_feature    729..749
                     /label=new 5-linker
     CDS             750..1448
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    1460..1480
                     /label=Feature 50
     misc_feature    1475..2080
                     /label=12XMS2V5
     misc_feature    1475..1497
                     /label=MS2V5-1
     misc_feature    1528..1550
                     /label=MS2V5-2
     misc_feature    1581..1603
                     /label=MS2V5-3
     misc_feature    1634..1656
                     /label=MS2V5-4
     misc_feature    1687..1709
                     /label=MS2V5-5
     misc_feature    1740..1762
                     /label=MS2V5-6
     misc_feature    1756..1776
                     /label=Feature 51
     misc_feature    1793..1815
                     /label=MS2V5-7
     misc_feature    1846..1868
                     /label=MS2V5-8
     misc_feature    1899..1921
                     /label=MS2V5-9
     misc_feature    1952..1974
                     /label=MS2V5-10
     misc_feature    2005..2027
                     /label=MS2V5-11
     misc_feature    2058..2080
                     /label=MS2V5-12
     misc_feature    2099..2119
                     /label=Feature 54
     misc_feature    2120..2140
                     /label=3' Linker-NEW
     misc_feature    2144..2665
                     /label=HSPB1 3'arm
     misc_feature    2637..2638
                     /label=Delete
     misc_feature    2672..2691
                     /label=sgTS2
     misc_feature    2692..2694
                     /label=PAM
     misc_feature    2700..2720
                     /label=Feature 20
     rep_origin      complement(3032..3617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3788..4648)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4649..4753)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattccatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtggagctt
      241 actgagactc ttcggggaat tctcctggct gcttccaagc aggaggtggg gcctctggcc
      301 tagcggggcc gaaaggcagt cccctccccc gcagtctgat ttccctcttc cccccaaagg
      361 caagcacgag gagcggcagg acgagcatgg ctacatctcc cggtgcttca cgcggaaata
      421 cacgtgagtc ctggcgccag gtcggggtgg gtgggtggcg tgggggtggg gtcagggaag
      481 agggcacagg gacccacccg gtgtgtaatg taacgcttgc ctttcctctc tgcacgtcca
      541 ggctgccccc cggtgtggac cccacccaag tttcctcctc cctgtcccct gagggcacac
      601 tgaccgtgga ggcccccatg cccaagctag ccacgcagtc caacgagatc accatcccag
      661 tcaccttcga gtcgcgggcc cagcttgggg gcccagaagc tgcaaaatcc gatgagactg
      721 ccgccaaggg atcgggtggt accagtggca tgagcgagct gattaaggag aacatgcaca
      781 tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg
      841 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
      901 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
      961 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
     1021 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     1081 aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc
     1141 ctgtgatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg
     1201 acggcggcct ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga
     1261 tcgcaaacat caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg
     1321 gcgtctacta tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg
     1381 tcgagcagca cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca
     1441 agcttaatta agcggccgcc cggtaaccta caaacgggtg gaggatcacc ccacccgaca
     1501 cttcacaatc aaggggtaca atacacaagg gtggaggaac accccaccct ccagacacat
     1561 tacacagaaa tccaatcaaa cagaagcacc atcagggctt ctgctaccaa atttatctca
     1621 aaaaactaca acaaggaatc accatcaggg attccctgtg caatatacgt caaacgaggg
     1681 ccacgacggg aggacgatca cgcctcccga atatcggcat gtctggcttt cgaattcagt
     1741 gcgtggagca tcagcccacg cagccaatca gagtcgaata caagtcgact ttcgcgaaga
     1801 gcatcagcct tcgcgccatt cttacacaaa ccacactctc ccctacagga acagcatcag
     1861 cgttcctgcc cagtacccaa ctcaagaaaa tttatgtccc catgcagcat cagcgcatgg
     1921 gccccaagaa tacatcccca acaaaatcac atccgagcac caacagggct cggagtgttg
     1981 tttcttgtcc aactggacaa accctccatg gaccatcagg ccatggactc tcaccaacaa
     2041 gacaaaaact actcttctcg aagcagcatc agcgcttcga aacactcgag catacattgt
     2101 gcctatttct tgggtggacg gaaccggtgc tggatccggt tagagcctta gcccggatgc
     2161 ccacccctgc tgccgccact ggctgtgcct cccccgccac ctgtgtgttc ttttgataca
     2221 tttatcttct gtttttctca aataaagttc aaagcaacca cctgtcactg gcccaggccc
     2281 tggtgtttgt ggaaggaagc ctcaggcacc tgccatttgc tggctttcag gagtcatctt
     2341 tgctcaggcc cgtgctgggc catgtgggta cactggtgta ggttgctgga cacaggctga
     2401 ctcacatcca taaagacaga ggtcttaggg ccgggcgcag tggctcatac ctacaatccc
     2461 agcactttgg ggggttgaag caggaggagt gcttgaagcc aagagttcta gaccagcctg
     2521 gacaacatag taagactgtc tctaaaaaat aaaaattagg cagggtggta ctgcacgcct
     2581 gtagtcccag ctactcagga ggctgaggca ggaggatcgc ttgagcccag gagttgtgaa
     2641 gggtacagtg agctaacatc gtgccgatat cggagcttac tgagactctt cgggcccggg
     2701 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     2761 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     2821 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     2881 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     2941 agccagtctg caggtcgacg gtctcgactc acatgtgagc aaaaggccag caaaaggcca
     3001 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3061 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3121 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3181 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3241 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3301 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3361 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3421 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3481 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3541 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3901 ctggccccag tgctgcaatg ataccgcgag aaccacgctc accggctcca gatttatcag
     3961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
