LOCUS       Exported                5118 bp ds-DNA     circular SYN 05-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Baohui
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          263..728
                     /organism="Homo sapiens"
                     /chromosome="7"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
     source          2478..2999
                     /organism="Homo sapiens"
                     /chromosome="7"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:9606"
     primer_bind     150..170
                     /label=HYP244
     misc_feature    234..253
                     /label=sgTS2
     misc_feature    254..256
                     /label=PAM
     gene            263..728
                     /gene="HSPB1"
                     /gene_synonym="CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; 
                     HSP27; HSP28; SRP27"
                     /label=HSPB1 5'arm
                     /note="heat shock protein family B (small) member 1; 
                     Derived by automated computational analysis using gene 
                     prediction method: BestRefSeq."
                     /db_xref="GeneID:3315"
                     /db_xref="HGNC:HGNC:5246"
                     /db_xref="MIM:602195"
     CDS             join(360..423,542..728)
                     /codon_start=1
                     /gene="HSPB1"
                     /gene_synonym="CMT2F; HEL-S-102; HMN2B; HS.76067; Hsp25; 
                     HSP27; HSP28; SRP27"
                     /product="heat shock protein beta-1"
                     /label=HSPB1
                     /note="Derived by automated computational analysis using 
                     gene prediction method: BestRefSeq."
                     /db_xref="CCDS:CCDS5583.1"
                     /db_xref="GeneID:3315"
                     /db_xref="HGNC:HGNC:5246"
                     /db_xref="MIM:602195"
                     /protein_id="NP_001531.1"
                     /translation="KHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPEGTLTVEA
                     PMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK"
     misc_feature    729..749
                     /label=new 5-linker
     misc_feature    750..2453
                     /label=NarTag
     misc_feature    750..1085
                     /label=TagBFP-N
     misc_feature    1086..2090
                     /label=Intron
     misc_feature    1169..1193
                     /label=PP7
     misc_feature    1198..1219
                     /label=1367, TS1
     misc_feature    1224..1248
                     
     misc_feature    1253..1274
                     /label=1367
     misc_feature    1279..1303
                     /label=PP7
     misc_feature    1308..1329
                     /label=1367
     misc_feature    1334..1358
                     
     misc_feature    1363..1384
                     /label=1367
     misc_feature    1464..1488
                     /label=PP7
     misc_feature    1493..1514
                     /label=1367
     misc_feature    1519..1543
                     
     misc_feature    1548..1569
                     /label=1367
     misc_feature    1574..1598
                     /label=PP7
     misc_feature    1603..1624
                     /label=1367
     misc_feature    1629..1653
                     
     misc_feature    1658..1679
                     /label=1367
     misc_feature    1759..1783
                     /label=PP7
     misc_feature    1788..1809
                     /label=1367
     misc_feature    1814..1838
                     
     misc_feature    1843..1864
                     /label=1367
     misc_feature    1869..1893
                     /label=PP7
     misc_feature    1898..1919
                     /label=1367
     misc_feature    1924..1948
                     
     misc_feature    1953..1974
                     /label=1367
     misc_feature    2091..2453
                     /label=TagBFP-C
     misc_feature    2454..2474
                     /label=3' Linker-NEW
     misc_feature    2475..2477
                     
     misc_feature    2478..2999
                     /label=HSPB1 3'arm
     misc_feature    2971..2972
                     /label=Delete
     misc_feature    3006..3025
                     /label=sgTS2
     misc_feature    3026..3028
                     /label=PAM
     primer_bind     complement(3126..3145)
                     /label=HYP245
     rep_origin      complement(3366..3951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4122..4982)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4983..5087)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattccatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtggagctt
      241 actgagactc ttcggggaat tctcctggct gcttccaagc aggaggtggg gcctctggcc
      301 tagcggggcc gaaaggcagt cccctccccc gcagtctgat ttccctcttc cccccaaagg
      361 caagcacgag gagcggcagg acgagcatgg ctacatctcc cggtgcttca cgcggaaata
      421 cacgtgagtc ctggcgccag gtcggggtgg gtgggtggcg tgggggtggg gtcagggaag
      481 agggcacagg gacccacccg gtgtgtaatg taacgcttgc ctttcctctc tgcacgtcca
      541 ggctgccccc cggtgtggac cccacccaag tttcctcctc cctgtcccct gagggcacac
      601 tgaccgtgga ggcccccatg cccaagctag ccacgcagtc caacgagatc accatcccag
      661 tcaccttcga gtcgcgggcc cagcttgggg gcccagaagc tgcaaaatcc gatgagactg
      721 ccgccaaggg atcgggtggt accagtggca tgagcgagct gattaaggag aacatgcaca
      781 tgaagctgta catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg
      841 aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc
      901 tccccttcgc cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca
      961 accacaccca gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg
     1021 agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc
     1081 aggacgtaag tatgaaattc agggatacgg ccaccttgtt ggccagatat acgcgttgac
     1141 gttagtaccg ggcccgctct agtacaaacc agcagagcat atgggctcgc tggacacgca
     1201 ccgatgctct ccgaggaggc acaggagcag acgatatggc gtcgctcccc aggcaccgat
     1261 gctctccgag gaggcaaacc agcagagcat atgggctcgc tggctacgca ccgatgctct
     1321 ccgaggagga acaggagcag acgatatggc gtcgctcctg tggcaccgat gctctccgag
     1381 gaggacgagc cgcgtggata accgtattac gggctaattc actcccaacg agcaaggtag
     1441 taccgggccc gctctagtac aaaccagcag agcatatggg ctcgctggac acgcaccgat
     1501 gctctccgag gaggcacagg agcagacgat atggcgtcgc tccccaggca ccgatgctct
     1561 ccgaggaggc aaaccagcag agcatatggg ctcgctggct acgcaccgat gctctccgag
     1621 gaggaacagg agcagacgat atggcgtcgc tcctgtggca ccgatgctct ccgaggagga
     1681 cgagccgcgt ggataaccgt attacccgct tgttacaccc tgtgagtgtc gttagtaccg
     1741 ggcccgctct agtacaaacc agcagagcat atgggctcgc tggacacgca ccgatgctct
     1801 ccgaggaggc acaggagcag acgatatggc gtcgctcccc aggcaccgat gctctccgag
     1861 gaggcaaacc agcagagcat atgggctcgc tggctacgca ccgatgctct ccgaggagga
     1921 acaggagcag acgatatggc gtcgctcctg tggcaccgat gctctccgag gaggacgagc
     1981 cgcgtggata accgtattac actcacatgt gagcccacct tgttggcata tttgccaaat
     2041 agtggaaatg tgaagtactg acaaaacttt tccctttttc aatctaatag ggctgcctca
     2101 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
     2161 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
     2221 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga
     2281 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
     2341 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
     2401 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aatggaaccg
     2461 gtgctggatc cggttagagc cttagcccgg atgcccaccc ctgctgccgc cactggctgt
     2521 gcctcccccg ccacctgtgt gttcttttga tacatttatc ttctgttttt ctcaaataaa
     2581 gttcaaagca accacctgtc actggcccag gccctggtgt ttgtggaagg aagcctcagg
     2641 cacctgccat ttgctggctt tcaggagtca tctttgctca ggcccgtgct gggccatgtg
     2701 ggtacactgg tgtaggttgc tggacacagg ctgactcaca tccataaaga cagaggtctt
     2761 agggccgggc gcagtggctc atacctacaa tcccagcact ttggggggtt gaagcaggag
     2821 gagtgcttga agccaagagt tctagaccag cctggacaac atagtaagac tgtctctaaa
     2881 aaataaaaat taggcagggt ggtactgcac gcctgtagtc ccagctactc aggaggctga
     2941 ggcaggagga tcgcttgagc ccaggagttg tgaagggtac agtgagctaa catcgtgccg
     3001 atatcggagc ttactgagac tcttcgggcc cgggcgacgg atggtgatcc ccctggccag
     3061 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     3121 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     3181 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     3241 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gacggtctcg
     3301 actcacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3361 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3421 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3481 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3541 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3601 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3661 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3721 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3781 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3841 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3901 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     3961 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4021 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4081 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4141 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4201 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4261 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4321 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4381 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4441 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4501 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4561 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4621 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4681 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4741 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4801 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4861 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4921 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4981 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5041 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5101 aaagtgccac ctgacgtc
//
