LOCUS       Exported               13832 bp ds-DNA     circular SYN 05-MAR-2023
DEFINITION  Lentivector that expresses H2B-Dendra2 from internal CAG 
            enhancer-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti.CAG.H2B-Dendra2.W
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13832)
  TITLE     Lentivirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13832
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             236..416
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1807..1924
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2034..2337
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2340..2615
                     /label=chicken beta-actin promoter
     intron          2616..3625
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     CDS             3750..3770
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    3780..7058
                     /label=dCas13b
     primer_bind     4285..4304
                     /label=sequencing_dPspCas13b_1
     primer_bind     complement(4406..4426)
                     /label=sequencing_dPspCas13b_6
     primer_bind     4905..4925
                     /label=sequencing_dPspCas13b_2
     primer_bind     6147..6167
                     /label=sequencing_dPspCas13b_4
     primer_bind     6768..6788
                     /label=sequencing_dPspCas13b_5
     CDS             7074..7094
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7101..7250
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7275..7295
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7725..8081
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             8100..8669
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             8676..8729
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8730..9611
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     primer_bind     8841..8861
                     /label=sp139-sep-f7
     misc_feature    9686..10274
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9739..9759)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10157..10168)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10361..10594
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10666..10787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10703..10722)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10757..10776
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10806..10941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10973..10992)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10974..10992)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11002..11019)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11002..11018)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11011..11033)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11163..11618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11250..11269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11460..11481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11647..11751
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11752..12612
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11970..11989)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12783..13371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13272..13291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13525..13542
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13659..13680
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        13695..13725
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13733..13749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13738..13760
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13757..13773
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13757..13773
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13792..13812
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13794..13812
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1921 ttttatcgat cacgagacta gcctcgagaa gcttgataat tctgcaggac ttctagttat
     1981 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2041 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2101 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2161 gactatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2221 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2281 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgggt
     2341 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
     2401 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggc
     2461 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     2521 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     2581 ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgttg ccttcgcccc
     2641 gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
     2701 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
     2761 gacggctcgt ttcttttctg tggctgcgtg aaagccttaa agggctccgg gagggccctt
     2821 tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg
     2881 tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
     2941 tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg ggggggctgc
     3001 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
     3061 gcggcggtcg ggctgtaacc cccccctgca cccccctccc cgagttgctg agcacggccc
     3121 ggcttcgggt gcggggctcc gtagcggggc gtggcgcggg gctcgccgtg ccgggcgggg
     3181 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     3241 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     3301 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg
     3361 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg
     3421 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgg cgccgtcccc
     3481 ttctccatct ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg
     3541 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     3601 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca
     3661 tcattttggc aaagaattga tttgataccg cgggcccggg atctcgagct caagcttcga
     3721 attctgcagt cgacggtacc gccaccatgc ccaagaagaa gcgcaaggtg ggacgcgtca
     3781 acatccccgc tctggtggaa aaccagaaga agtactttgg cacctacagc gtgatggcca
     3841 tgctgaacgc tcagaccgtg ctggaccaca tccagaaggt ggccgatatt gagggcgagc
     3901 agaacgagaa caacgagaat ctgtggtttc accccgtgat gagccacctg tacaacgcca
     3961 agaacggcta cgacaagcag cccgagaaaa ccatgttcat catcgagcgg ctgcagagct
     4021 acttcccatt cctgaagatc atggccgaga accagagaga gtacagcaac ggcaagtaca
     4081 agcagaaccg cgtggaagtg aacagcaacg acatcttcga ggtgctgaag cgcgccttcg
     4141 gcgtgctgaa gatgtacagg gacctgacca acgcatacaa gacctacgag gaaaagctga
     4201 acgacggctg cgagttcctg accagcacag agcaacctct gagcggcatg atcaacaact
     4261 actacacagt ggccctgcgg aacatgaacg agagatacgg ctacaagaca gaggacctgg
     4321 ccttcatcca ggacaagcgg ttcaagttcg tgaaggacgc ctacggcaag aaaaagtccc
     4381 aagtgaatac cggattcttc ctgagcctgc aggactacaa cggcgacaca cagaagaagc
     4441 tgcacctgag cggagtggga atcgccctgc tgatctgcct gttcctggac aagcagtaca
     4501 tcaacatctt tctgagcagg ctgcccatct tctccagcta caatgcccag agcgaggaac
     4561 ggcggatcat catcagatcc ttcggcatca acagcatcaa gctgcccaag gaccggatcc
     4621 acagcgagaa gtccaacaag agcgtggcca tggatatgct caacgaagtg aagcggtgcc
     4681 ccgacgagct gttcacaaca ctgtctgccg agaagcagtc ccggttcaga atcatcagcg
     4741 acgaccacaa tgaagtgctg atgaagcgga gcagcgacag attcgtgcct ctgctgctgc
     4801 agtatatcga ttacggcaag ctgttcgacc acatcaggtt ccacgtgaac atgggcaagc
     4861 tgagatacct gctgaaggcc gacaagacct gcatcgacgg ccagaccaga gtcagagtga
     4921 tcgagcagcc cctgaacggc ttcggcagac tggaagaggc cgagacaatg cggaagcaag
     4981 agaacggcac cttcggcaac agcggcatcc ggatcagaga cttcgagaac atgaagcggg
     5041 acgacgccaa tcctgccaac tatccctaca tcgtggacac ctacacacac tacatcctgg
     5101 aaaacaacaa ggtcgagatg tttatcaacg acaaagagga cagcgcccca ctgctgcccg
     5161 tgatcgagga tgatagatac gtggtcaaga caatccccag ctgccggatg agcaccctgg
     5221 aaattccagc catggccttc cacatgtttc tgttcggcag caagaaaacc gagaagctga
     5281 tcgtggacgt gcacaaccgg tacaagagac tgttccaggc catgcagaaa gaagaagtga
     5341 ccgccgagaa tatcgccagc ttcggaatcg ccgagagcga cctgcctcag aagatcctgg
     5401 atctgatcag cggcaatgcc cacggcaagg atgtggacgc cttcatcaga ctgaccgtgg
     5461 acgacatgct gaccgacacc gagcggagaa tcaagagatt caaggacgac cggaagtcca
     5521 ttcggagcgc cgacaacaag atgggaaaga gaggcttcaa gcagatctcc acaggcaagc
     5581 tggccgactt cctggccaag gacatcgtgc tgtttcagcc cagcgtgaac gatggcgaga
     5641 acaagatcac cggcctgaac taccggatca tgcagagcgc cattgccgtg tacgatagcg
     5701 gcgacgatta cgaggccaag cagcagttca agctgatgtt cgagaaggcc cggctgatcg
     5761 gcaagggcac aacagagcct catccatttc tgtacaaggt gttcgcccgc agcatccccg
     5821 ccaatgccgt cgagttctac gagcgctacc tgatcgagcg gaagttctac ctgaccggcc
     5881 tgtccaacga gatcaagaaa ggcaacagag tggatgtgcc cttcatccgg cgggaccaga
     5941 acaagtggaa aacacccgcc atgaagaccc tgggcagaat ctacagcgag gatctgcccg
     6001 tggaactgcc cagacagatg ttcgacaatg agatcaagtc ccacctgaag tccctgccac
     6061 agatggaagg catcgacttc aacaatgcca acgtgaccta tctgatcgcc gagtacatga
     6121 agagagtgct ggacgacgac ttccagacct tctaccagtg gaaccgcaac taccggtaca
     6181 tggacatgct taagggcgag tacgacagaa agggctccct gcagcactgc ttcaccagcg
     6241 tggaagagag agaaggcctc tggaaagagc gggcctccag aacagagcgg tacagaaagc
     6301 aggccagcaa caagatccgc agcaaccggc agatgagaaa cgccagcagc gaagagatcg
     6361 agacaatcct ggataagcgg ctgagcaaca gccggaacga gtaccagaaa agcgagaaag
     6421 tgatccggcg ctacagagtg caggatgccc tgctgtttct gctggccaaa aagaccctga
     6481 ccgaactggc cgatttcgac ggcgagaggt tcaaactgaa agaaatcatg cccgacgccg
     6541 agaagggaat cctgagcgag atcatgccca tgagcttcac cttcgagaaa ggcggcaaga
     6601 agtacaccat caccagcgag ggcatgaagc tgaagaacta cggcgacttc tttgtgctgg
     6661 ctagcgacaa gaggatcggc aacctgctgg aactcgtggg cagcgacatc gtgtccaaag
     6721 aggatatcat ggaagagttc aacaaatacg accagtgcag gcccgagatc agctccatcg
     6781 tgttcaacct ggaaaagtgg gccttcgaca cataccccga gctgtctgcc agagtggacc
     6841 gggaagagaa ggtggacttc aagagcatcc tgaaaatcct gctgaacaac aagaacatca
     6901 acaaagagca gagcgacatc ctgcggaaga tccggaacgc cttcgatgca aacaattacc
     6961 ccgacaaagg cgtggtggaa atcaaggccc tgcctgagat cgccatgagc atcaagaagg
     7021 cctttgggga gtacgccatc atgaagggaa gcctgcagtc tagagaggga gctcccaaga
     7081 aaaagcgcaa ggtaactagt gacgcattgg acgattttga tctggatatg ctgggaagtg
     7141 acgccctcga tgattttgac cttgacatgc ttggttcgga tgcccttgat gactttgacc
     7201 tcgacatgct cggcagtgac gcccttgatg atttcgacct ggacatgctg attaactcta
     7261 gaagttccgg atctccgaaa aagaaacgca aagttggtag ccagtacctg cccgacaccg
     7321 acgaccggca ccggatcgag gaaaagcgga agcggaccta cgagacattc aagagcatca
     7381 tgaagaagtc ccccttcagc ggccccaccg accctagacc tccacctaga agaatcgccg
     7441 tgcccagcag atccagcgcc agcgtgccaa aacctgcccc ccagccttac cccttcacca
     7501 gcagcctgag caccatcaac tacgacgagt tccctaccat ggtgttcccc agcggccaga
     7561 tctctcaggc ctctgctctg gctccagccc ctcctcaggt gctgcctcag gctcctgctc
     7621 ctgcaccagc tccagccatg gtgtctgcac tggctcaggc accagcaccc gtgcctgtgc
     7681 tggctcctgg acctccacag gctgtggctc caccagcccc taaacctaca caggccggcg
     7741 agggcacact gtctgaagct ctgctgcagc tgcagttcga cgacgaggat ctgggagccc
     7801 tgctgggaaa cagcaccgat cctgccgtgt tcaccgacct ggccagcgtg gacaacagcg
     7861 agttccagca gctgctgaac cagggcatcc ctgtggcccc tcacaccacc gagcccatgc
     7921 tgatggaata ccccgaggcc atcacccggc tcgtgacagg cgctcagagg cctcctgatc
     7981 cagctcctgc ccctctggga gcaccaggcc tgcctaatgg actgctgtct ggcgacgagg
     8041 acttcagctc tatcgccgat atggatttct cagccttgct gggctctggc agcggcagcc
     8101 gggattccag ggaagggatg tttttgccga agcctgaggc cggctccgct attagtgacg
     8161 tgtttgaggg ccgcgaggtg tgccagccaa aacgaatccg gccatttcat cctccaggaa
     8221 gtccatgggc caaccgccca ctccccgcca gcctcgcacc aacaccaacc ggtccagtac
     8281 atgagccagt cgggtcactg accccggcac cagtccctca gccactggat ccagcgcccg
     8341 cagtgactcc cgaggccagt cacctgttgg aggatcccga tgaagagacg agccaggctg
     8401 tcaaagccct tcgggagatg gccgatactg tgattcccca gaaggaagag gctgcaatct
     8461 gtggccaaat ggacctttcc catccgcccc caaggggcca tctggatgag ctgacaacca
     8521 cacttgagtc catgaccgag gatctgaacc tggactcacc cctgaccccg gaattgaacg
     8581 agattctgga taccttcctg aacgacgagt gcctcttgca tgccatgcat atcagcacag
     8641 gactgtccat cttcgacaca tctctgtttg gatccgaggg cagaggaagt ctgctaacat
     8701 gcggtgacgt cgaggagaat cctggcccag aaatcggtac tggctttcca ttcgaccccc
     8761 attatgtgga agtcctgggc gagcgcatgc actacgtcga tgttggtccg cgcgatggca
     8821 cccctgtgct gttcctgcac ggtaacccga cctcctccta cgtgtggcgc aacatcatcc
     8881 cgcatgttgc accgacccat cgctgcattg ctccagacct gatcggtatg ggcaaatccg
     8941 acaaaccaga cctgggttat ttcttcgacg accacgtccg cttcatggat gccttcatcg
     9001 aagccctggg tctggaagag gtcgtcctgg tcattcacga ctggggctcc gctctgggtt
     9061 tccactgggc caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc
     9121 gccctatccc gacctgggac gaatggccag aatttgcccg cgagaccttc caggccttcc
     9181 gcaccaccga cgtcggccgc aagctgatca tcgatcagaa cgtttttatc gagggtacgc
     9241 tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat ggaccattac cgcgagccgt
     9301 tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc aaacgagctg ccaatcgccg
     9361 gtgagccagc gaacatcgtc gcgctggtcg aagaatacat ggactggctg caccagtccc
     9421 ctgtcccgaa gctgctgttc tggggcaccc caggcgttct gatcccaccg gccgaagccg
     9481 ctcgcctggc caaaagcctg cctaactgca aggctgtgga catcggcccg ggtctgaatc
     9541 tgctgcaaga agacaacccg gacctgatcg gcagcgagat cgcgcgctgg ctgtctactc
     9601 tggagatttc cggttaatgt acaagtgaaa gcttaattag ctgagcttgg actcctgttg
     9661 aaagggcgaa ttctgcagat tcgacaatca acctctggat tacaaaattt gtgaaagatt
     9721 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     9781 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     9841 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     9901 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     9961 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
    10021 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
    10081 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
    10141 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
    10201 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
    10261 ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
    10321 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
    10381 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
    10441 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10501 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10561 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
    10621 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
    10681 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10741 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
    10801 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    10861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    10921 gaggaggctt ttttggaggc ctaggctttt gcgtcgagac gtacccaatt cgccctatag
    10981 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
    11041 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
    11101 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
    11161 cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
    11221 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
    11281 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
    11341 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    11401 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    11461 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    11521 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    11581 taacgcgaat tttaacaaaa tattaacgtt tacaatttcc caggtggcac ttttcgggga
    11641 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    11701 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    11761 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    11821 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    11881 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    11941 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    12001 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    12061 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    12121 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    12181 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    12241 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    12301 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    12361 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    12421 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    12481 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    12541 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    12601 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    12661 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    12721 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    12781 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    12841 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    12901 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
    12961 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    13021 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    13081 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    13141 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    13201 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    13261 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    13321 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    13381 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    13441 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    13501 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    13561 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    13621 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
    13681 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
    13741 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
    13801 cctcactaaa gggaacaaaa gctggagctg ca
//
