LOCUS       Exported                5467 bp ds-DNA     circular SYN 05-MAR-2023
DEFINITION  Vector that allows high-level transient expression in vertebrate 
            cells as well as in vitro transcription/translation.
ACCESSION   .
VERSION     .
KEYWORDS    pCS2+
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   I.M.A.G.E. Consortium
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4060..4714
                     /organism="Danio rerio"
                     /chromosome="14"
                     /strain="Tuebingen"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:7955"
     primer_bind     complement(1..17)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    25..41
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(49..79)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(403..991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1162..2022)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2023..2127)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2153..2608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2682..3666
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     primer_bind     3647..3667
                     /label=LYP561:PLY194-seq1-F
     promoter        3701..3719
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    3757..5131
                     /label=Zebrafish NarTag
     misc_feature    3757..3759
                     /label=ATG
     misc_feature    3760..4059
                     /label=EGFP-N
     primer_bind     4039..4059
                     /label=LYP563:EGFP-DF7-seq
     misc_feature    4060..4714
                     /label=Intron
     misc_feature    4136..4158
                     /label=MS2V5_1
     misc_feature    4189..4211
                     /label=MS2
     misc_feature    4242..4264
                     /label=MS2
     misc_feature    4295..4317
                     /label=MS2
     misc_feature    4348..4370
                     /label=MS2
     misc_feature    4401..4423
                     /label=MS2
     misc_feature    4454..4476
                     /label=MS2
     misc_feature    4507..4529
                     /label=MS2
     misc_feature    4560..4582
                     /label=MS2
     misc_feature    4715..5131
                     /label=EGFP-C
     polyA_signal    5182..5316
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5215..5238)
                     /label=LYP560:PLY194-seq3-R
     promoter        complement(5429..5447)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
       61 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      121 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      181 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      241 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      301 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      361 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
      421 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      481 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      541 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      601 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      661 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      721 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      781 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      841 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      901 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      961 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1021 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1081 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1141 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     1201 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     1261 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     1321 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1381 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1441 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1501 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1561 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1621 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1681 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1741 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1801 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1861 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1921 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1981 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2041 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2101 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     2161 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     2221 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     2281 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     2341 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     2401 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2461 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2521 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2581 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     2641 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt cgaccatagc caattcaata
     2701 tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata
     2761 tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata
     2821 tggcggactt ggcaccatgc caattcaata tggcggactt ggcactgtgc caactgggga
     2881 ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc
     2941 catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat
     3001 atataggcaa tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta
     3061 tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc
     3121 ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg cccatagcca
     3181 ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg
     3241 gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct
     3301 ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca
     3361 ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac
     3421 ttggcagtac atcaatatct attaatagta acttggcaag tacattacta ttggaaggac
     3481 gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc
     3541 tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca
     3601 ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta
     3661 gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga cactatagaa
     3721 tacaagctac ttgttctttt tgcaggatcc gccaccatgg tgagcaaggg cgaggagctg
     3781 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3841 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3901 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3961 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4021 atgcccgaag gctacgtcca ggagcgcacc atcttcttcg taagtatcta tctttctttt
     4081 agttactctc tattccacct tgttggcact tcacaatcaa ggggtacaat acacaagggt
     4141 ggaggaacac cccaccctcc agacacatta cacagaaatc caatcaaaca gaagcaccat
     4201 cagggcttct gctaccaaat ttatctcaaa aaactacaac aaggaatcac catcagggat
     4261 tccctgtgca atatacgtca aacgagggcc acgacgggag gacgatcacg cctcccgaat
     4321 atcggcatgt ctggctttcg aattcagtgc gtggagcatc agcccacgca gccaatcaga
     4381 gtcgaataca agtcgacttt cgcgaagagc atcagccttc gcgccattct tacacaaacc
     4441 acactctccc ctacaggaac agcatcagcg ttcctgccca gtacccaact caagaaaatt
     4501 tatgtcccca tgcagcatca gcgcatgggc cccaagaata catccccaac aaaatcacat
     4561 ccgagcacca acagggctcg gagtgttgtt tcttgtccaa ctggacaaac ccccaccttg
     4621 ttggagattt aacagcctac atgctagaga aactctcaac tactttattt ttgttgaata
     4681 tctgctaatt ttctttccca ccatctcttc ccagaaggac gacggcaact acaagacccg
     4741 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     4801 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     4861 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     4921 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     4981 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     5041 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5101 cactctcggc atggacgagc tgtacaagta agaattcaag gcctctcgag cctctagaac
     5161 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
     5221 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     5281 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     5341 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg
     5401 cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     5461 aatcatg
//
