LOCUS       Exported                5199 bp ds-DNA     circular SYN 04-MAR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    205..210
                     /label=RBS
                     /note="color: #a6acb3"
     CDS             219..227
                     /codon_start=1
                     /label=Linker
                     /note="color: #a6acb3"
                     /translation="MGS"
     CDS             228..245
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             246..254
                     /codon_start=1
                     /label=Linker
                     /note="color: #a6acb3"
                     /translation="GRS"
     CDS             255..1451
                     /codon_start=1
                     /label=BpBBS
                     /note="color: #0000ff"
                     /translation="HHEEGHHDHEDLKDDHDPFLPEDHKKALFVYQKPALNNINFAFKM
                     YRQLARDHPTENIVISPVSISSALALLSLGAKGHTHSQIVERLGYNTSEIPEQQIHESF
                     HKQLDVVDDKDRDLEFEHGNALFTCKEHKIHQTFLDDAKKFYHSEVIPTDFKNTEEAKN
                     QINSYVEKSTHGKITNILDSVDQDAMIALINFIYLRANWQHPFDEKLTKEGDFHVDKDT
                     TVKVPFMRRRGIYKMAYTDDIIMVTIPYNGSVEMFLAMTKMGKLSELEQNLNRERSLKW
                     REIMQYQLIDLSLPKLSVSGILNLKETLSKLGIVDVFSNHADLSGITDESHLKVSKAIH
                     KAMMSFDEHGTEAAPATAAEADPLMLPPHFKFDYPFIFRVQDLKTKNPLLVGRIANPQK
                     "
     CDS             1293..1358
                     /label=R-loop
                     /note="color: #c0c0c0; direction: RIGHT"
     terminator      1670..1756
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     terminator      1848..1875
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        1894..1985
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1986..2846
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 1986 .. 2054 / #ccffcc / signal sequence
                        2: 2055 .. 2846 / #ccffcc
                     Cleavage site after base 2054"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3017..3605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     misc_feature    3791..3931
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     CDS             complement(4195..5073)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /note="color: #993366"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        join(5100..5199,1..185)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
       61 cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg ccatagcatt
      121 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
      181 tccatacccg ttttttgggc taacaggagg aattaaccat ggggagccac catcaccatc
      241 accatggcag atctcaccac gaggagggac accacgacca cgaggatctg aaggacgatc
      301 acgacccatt cctgcccgag gatcacaaga aggccctgtt cgtgtaccag aagcccgccc
      361 tgaacaatat caacttcgcc tttaagatgt atagacagct ggccagggac caccctacag
      421 agaatatcgt gatctcccca gtgtctatca gctccgccct ggccctgctg agcctgggag
      481 caaagggaca cacccactcc cagatcgtgg agagactggg ctacaacaca tctgagatcc
      541 ctgagcagca gatccacgag agcttccaca agcagctgga cgtggtggac gataaggaca
      601 gggatctgga gttcgagcac ggcaatgccc tgtttacctg caaggagcac aagatccacc
      661 agacattcct ggacgatgcc aagaagtttt accactctga agtgatccca accgacttca
      721 agaacacaga ggaggccaag aaccagatca atagctatgt ggagaagtcc acccacggca
      781 agatcacaaa tatcctggat agcgtggacc aggatgccat gatcgccctg atcaacttta
      841 tctatctgcg cgccaattgg cagcaccctt tcgacgagaa gctgaccaag gagggcgatt
      901 ttcacgtgga caaggatacc acagtgaagg tgccattcat gcggagaagg ggcatctaca
      961 agatggccta taccgacgat atcatcatgg tgacaatccc ctacaacggc tccgtggaga
     1021 tgtttctggc catgacaaag atgggcaagc tgtccgagct ggagcagaac ctgaatcgcg
     1081 agcggtctct gaagtggcgg gagatcatgc agtatcagct gatcgacctg agcctgccca
     1141 agctgtctgt gagcggcatc ctgaacctga aggagaccct gtccaagctg ggcatcgtgg
     1201 acgtgttcag caatcacgcc gacctgtctg gcatcacaga tgagtctcac ctgaaggtga
     1261 gcaaggccat ccacaaggcc atgatgtctt ttgacgagca cggaaccgag gcagcaccag
     1321 caacagcagc agaggcagat cctctgatgc tgccacctca cttcaagttt gactaccctt
     1381 tcatctttcg cgtgcaggat ctgaaaacca agaacccact gctggtgggc cggatcgcca
     1441 atccccagaa gtgagaattc gaagcttggc tgttttggcg gatgagagaa gattttcagc
     1501 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc
     1561 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
     1621 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     1681 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1741 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     1801 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
     1861 gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa
     1921 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1981 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2041 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2101 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2161 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2221 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2281 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2341 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2401 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2461 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2521 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2581 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2641 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2701 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2761 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2821 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2881 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2941 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3001 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3061 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3781 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3841 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     3901 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     3961 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4021 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa
     4081 ttcgcgcgcg aaggcgaagc ggcatgcata atgtgcctgt caaatggacg aagcagggat
     4141 tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga
     4201 caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct
     4261 ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt
     4321 gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat
     4381 acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga
     4441 cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc
     4501 tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg
     4561 gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc
     4621 cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc
     4681 gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga
     4741 cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata
     4801 ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa
     4861 aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg
     4921 aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag
     4981 ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc
     5041 cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga
     5101 agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt
     5161 ctcgctaacc aaaccggtaa ccccgcttat taaaagcat
//
