LOCUS       Exported                5610 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_feature    432..479
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    480..499
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    510..529
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    533..580
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     intron          581..742
                     /locus_tag="B-actin intron 3'"
                     /label=B-actin intron 3'
     variation       743..787
                     /locus_tag="flexible linker (GGGGS)x3 zf-opt"
                     /label=flexible linker (GGGGS)x3 zf-opt
     variation       743..757
                     /locus_tag="(GGGGS)linker"
                     /label=(GGGGS)linker
     exon            743..745
                     /locus_tag="B-actin exon 2 start (SA_0)"
                     /label=B-actin exon 2 start (SA_0)
     variation       758..772
                     /locus_tag="(GGGGS)linker(1)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(1)
     variation       773..787
                     /locus_tag="(GGGGS)linker(2)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(2)
     misc_feature    788..1483
                     /locus_tag="mKate2"
                     /label=mKate2
     misc_feature    1490..3364
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    1490..1574
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      2055..3364
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(3385..3405)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(3402..3424)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(3411..3433)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(3773..4455)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(4550..5410)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(4553..5212)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tgaagttcct attccgaagt tcctattctt caaatagtat aggaacttcg
      481 aagatcggcc actacattct ggcctaggag gctgctgcgg ttccagaggt gggaagttcc
      541 tattccgaag ttcctattct ctagaaagta taggaacttc aataaaagtt aatttaagcg
      601 ttcattacaa taaattcaag tgaatctgtt ttaagctagc ctttaacgtt tttaaattcc
      661 ttcaaacaaa ttggatcctc tttgcagttc ctcatttctc ggtgagctgg tgctttaatc
      721 cctttacttt ttcttcctct agggaggagg aggatctgga ggaggaggat ctggaggagg
      781 aggatctatg tccgaactta tcaaagaaaa tatgcatatg aagctgtaca tggagggcac
      841 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
      901 ccagaccatg agaatcaagg cggtcgaggg cggccctctc cccttcgcct tcgacatcct
      961 ggctaccagc ttcatgtacg gcagcaaaac cttcatcaac cacacccagg gcatccccga
     1021 cttctttaag cagtccttcc ccgagggctt cacatgggag agagtcacca catacgaaga
     1081 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     1141 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
     1201 cggctgggag gcctccaccg agaccctgta ccccgctgac ggcggcctgg aaggcagagc
     1261 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttga agaccacata
     1321 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggacagaag
     1381 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt
     1441 ggccagatac tgcgacctcc ctagcaaact ggggcacaga taaactagta ttaagtctca
     1501 gccaccgtta actgaacatg tcaaaacctg tggagactgt tgagatttga tgttctgaaa
     1561 agataaagcc tataaataaa acaatactca aatacaaaag gtatcttttt taaaaagtta
     1621 gtttactgct tccttaggct ctttctaaat cccacccacc tgaaggacat caattatgga
     1681 tttatccttc atgccttgta taaatacaga tgtgcatatg aatttttatg ggttttttaa
     1741 aaatgtggtt cattctatat acataacaat tactttcaac atacataagc ctacctgtat
     1801 ttgtgcatta tattacatgg tacagataaa tagtcatgtg ccccatgaac attttggtca
     1861 aagatagatt acagatacaa tggtggtccc ataagattat gatgccatat ttttactgta
     1921 cctcttcttg tgcctagata cacaaatact gacttgtgtt ataattgcct gcagtattca
     1981 ataatatggt gtatagattt gtagcctacg agctataacc tatactatat agccaaggag
     2041 tgcaggaggg tatgccatct agatttgtgt gagtacactg atgtttgaac aataaaatca
     2101 cctaatgata ggtttctcaa accatatccc agtccttaag caacacatga ctaatggatt
     2161 caaccatttc ccctgttcgt tcagattgct ttttcttttt gcccctacaa ttttgttgat
     2221 aatactgttt tttggggctt ttgtttttgt tttttttttt ttttttgaga caagagtctc
     2281 actctgtcgt caggctggag tgcaatggcg ctatctcagc tcactgcaac ctctgcctcc
     2341 caggttcaag cgattctcct gcctcagcct cctgagtagc tgagattaca ggcatgcgcc
     2401 accgcgcccg gctaattttg tatttttagt agagacgcag tttcaccatg ttggtcaggc
     2461 tggtcttgaa cacccgacct caggtgatcc acccgcctca gcttcccaaa gtgctgggat
     2521 taaaggcgtg agccaccaca cctggccaat ttactgtttt ataccatgct tttttgtgtg
     2581 tattctaggt tccctaaagc aaacatgcat tttaaatttt gatagaacca gatgacatac
     2641 aaaagtgcct gttttctctg cattattaaa actgaaggta atcagtcttt tatttctatg
     2701 actccaactg gcaaaaaata tttgccattt taactttttt tttttgagac ggagtcaaaa
     2761 ggtgccagtt ttctctgcat tattaaaact gaaggtaatc agtcttttat ttctatgact
     2821 ccaactggca aaagatattt gccacgttaa cttttttttt tttttttttg agacggaggc
     2881 tcacactgtc acccaggctg aagtgcagtg gcatgatctc agctcacttg caacctccgc
     2941 ctcccaggtt caagtgattc tcctgcctca gcctcccaag gagttgggat tacaggcgca
     3001 tacccggctt attttttgta tttttagtag aaacgggatt tcaccgtgtt agccaggatg
     3061 gtctcaatct ccagacttcg tgatcgccca cctcggcctc ccaaagtgct gggattacag
     3121 gcgtgagcca ccgcgcccgg ccctcagtag gcgattttta tatgtggtat aaagtagaat
     3181 tcttacagat ggatacccgt ttatgccagt gccatgactg aaaccctgct tgtccaactt
     3241 gaaatatctt caatgcccat ctattcttcc ttgatcttgt tttttcctgt gataatacaa
     3301 tagtttttac cagttaatca gttaagatga ggcttaccaa atatatggga gtactactga
     3361 acacgatatc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3421 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3481 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3541 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     3601 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3961 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4201 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4261 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4441 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4501 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4561 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4621 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4681 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4741 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4801 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4861 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4921 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4981 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5041 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5101 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5161 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5221 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5281 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5341 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5401 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5461 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5521 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     5581 aaaataggcg tatcacgagg ccctttcgtc
//
