LOCUS       Exported                5611 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pSA_1_mKate2_synCoTC from 1 to 5611
            pSA_0_mKate2_synCoTC from 1 to 5610
            pSA_0_mKate2 from 1 to 4189
            0_FRT_FRT3_SA-mKate2-afpUTR_pUC57 from 1 to 4154
            FRT_SPACER_SgH-FRT3-SA-zfmTagBFP2-afpUTR_FINAL_pUC57 from 1 to 4819
            FRT-SgH-FRT3-SA-zfmTagBFP2-afpUTR_FINAL_pUC57 from 1 to 4217
FEATURES             Location/Qualifiers
     source          1..5611
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_feature    432..479
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    480..499
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    510..529
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    533..580
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     intron          581..742
                     /locus_tag="B-actin intron 3'"
                     /label=B-actin intron 3'
     exon            743..746
                     /locus_tag="B-actin exon 2 start (SA_1)"
                     /label=B-actin exon 2 start (SA_1)
     variation       744..788
                     /locus_tag="flexible linker (GGGGS)x3 zf-opt"
                     /label=flexible linker (GGGGS)x3 zf-opt
     variation       744..758
                     /locus_tag="(GGGGS)linker"
                     /label=(GGGGS)linker
     variation       759..773
                     /locus_tag="(GGGGS)linker(1)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(1)
     variation       774..788
                     /locus_tag="(GGGGS)linker(2)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(2)
     misc_feature    789..1484
                     /locus_tag="mKate2"
                     /label=mKate2
     misc_feature    1491..3365
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    1491..1575
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      2056..3365
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(3386..3406)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(3403..3425)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(3412..3434)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(3774..4456)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(4551..5411)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(4554..5213)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tgaagttcct attccgaagt tcctattctt caaatagtat aggaacttcg
      481 aagatcggcc actacattct ggcctaggag gctgctgcgg ttccagaggt gggaagttcc
      541 tattccgaag ttcctattct ctagaaagta taggaacttc aataaaagtt aatttaagcg
      601 ttcattacaa taaattcaag tgaatctgtt ttaagctagc ctttaacgtt tttaaattcc
      661 ttcaaacaaa ttggatcctc tttgcagttc ctcatttctc ggtgagctgg tgctttaatc
      721 cctttacttt ttcttcctct aggggaggag gaggatctgg aggaggagga tctggaggag
      781 gaggatctat gtccgaactt atcaaagaaa atatgcatat gaagctgtac atggagggca
      841 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
      901 cccagaccat gagaatcaag gcggtcgagg gcggccctct ccccttcgcc ttcgacatcc
      961 tggctaccag cttcatgtac ggcagcaaaa ccttcatcaa ccacacccag ggcatccccg
     1021 acttctttaa gcagtccttc cccgagggct tcacatggga gagagtcacc acatacgaag
     1081 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     1141 acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
     1201 tcggctggga ggcctccacc gagaccctgt accccgctga cggcggcctg gaaggcagag
     1261 ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttg aagaccacat
     1321 acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat gtggacagaa
     1381 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg
     1441 tggccagata ctgcgacctc cctagcaaac tggggcacag ataaactagt attaagtctc
     1501 agccaccgtt aactgaacat gtcaaaacct gtggagactg ttgagatttg atgttctgaa
     1561 aagataaagc ctataaataa aacaatactc aaatacaaaa ggtatctttt ttaaaaagtt
     1621 agtttactgc ttccttaggc tctttctaaa tcccacccac ctgaaggaca tcaattatgg
     1681 atttatcctt catgccttgt ataaatacag atgtgcatat gaatttttat gggtttttta
     1741 aaaatgtggt tcattctata tacataacaa ttactttcaa catacataag cctacctgta
     1801 tttgtgcatt atattacatg gtacagataa atagtcatgt gccccatgaa cattttggtc
     1861 aaagatagat tacagataca atggtggtcc cataagatta tgatgccata tttttactgt
     1921 acctcttctt gtgcctagat acacaaatac tgacttgtgt tataattgcc tgcagtattc
     1981 aataatatgg tgtatagatt tgtagcctac gagctataac ctatactata tagccaagga
     2041 gtgcaggagg gtatgccatc tagatttgtg tgagtacact gatgtttgaa caataaaatc
     2101 acctaatgat aggtttctca aaccatatcc cagtccttaa gcaacacatg actaatggat
     2161 tcaaccattt cccctgttcg ttcagattgc tttttctttt tgcccctaca attttgttga
     2221 taatactgtt ttttggggct tttgtttttg tttttttttt tttttttgag acaagagtct
     2281 cactctgtcg tcaggctgga gtgcaatggc gctatctcag ctcactgcaa cctctgcctc
     2341 ccaggttcaa gcgattctcc tgcctcagcc tcctgagtag ctgagattac aggcatgcgc
     2401 caccgcgccc ggctaatttt gtatttttag tagagacgca gtttcaccat gttggtcagg
     2461 ctggtcttga acacccgacc tcaggtgatc cacccgcctc agcttcccaa agtgctggga
     2521 ttaaaggcgt gagccaccac acctggccaa tttactgttt tataccatgc ttttttgtgt
     2581 gtattctagg ttccctaaag caaacatgca ttttaaattt tgatagaacc agatgacata
     2641 caaaagtgcc tgttttctct gcattattaa aactgaaggt aatcagtctt ttatttctat
     2701 gactccaact ggcaaaaaat atttgccatt ttaacttttt ttttttgaga cggagtcaaa
     2761 aggtgccagt tttctctgca ttattaaaac tgaaggtaat cagtctttta tttctatgac
     2821 tccaactggc aaaagatatt tgccacgtta actttttttt tttttttttt gagacggagg
     2881 ctcacactgt cacccaggct gaagtgcagt ggcatgatct cagctcactt gcaacctccg
     2941 cctcccaggt tcaagtgatt ctcctgcctc agcctcccaa ggagttggga ttacaggcgc
     3001 atacccggct tattttttgt atttttagta gaaacgggat ttcaccgtgt tagccaggat
     3061 ggtctcaatc tccagacttc gtgatcgccc acctcggcct cccaaagtgc tgggattaca
     3121 ggcgtgagcc accgcgcccg gccctcagta ggcgattttt atatgtggta taaagtagaa
     3181 ttcttacaga tggatacccg tttatgccag tgccatgact gaaaccctgc ttgtccaact
     3241 tgaaatatct tcaatgccca tctattcttc cttgatcttg ttttttcctg tgataataca
     3301 atagttttta ccagttaatc agttaagatg aggcttacca aatatatggg agtactactg
     3361 aacacgatat caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     3421 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     3481 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     3541 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3601 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3661 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3721 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3781 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3841 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3901 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3961 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4021 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4081 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4141 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4201 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4261 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4321 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4381 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4441 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4501 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4561 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4621 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4681 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4741 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4801 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4861 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4921 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4981 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5041 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5101 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5161 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5221 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5281 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5341 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5401 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5461 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5521 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     5581 aaaaataggc gtatcacgag gccctttcgt c
//
