LOCUS       Exported                5615 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pSA_synCoTC
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_feature    432..479
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    480..499
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    510..529
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    533..580
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     intron          581..742
                     /locus_tag="B-actin intron 3'"
                     /label=B-actin intron 3'
     exon            743..747
                     /locus_tag="B-actin exon 2 start (SA_2)"
                     /label=B-actin exon 2 start (SA_2)
     variation       745..789
                     /locus_tag="flexible linker (GGGGS)x3 zf-opt"
                     /label=flexible linker (GGGGS)x3 zf-opt
     variation       745..759
                     /locus_tag="(GGGGS)linker"
                     /label=(GGGGS)linker
     variation       760..774
                     /locus_tag="(GGGGS)linker(1)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(1)
     variation       775..789
                     /locus_tag="(GGGGS)linker(2)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(2)
     misc_feature    790..1488
                     /locus_tag="mKate2 (+2nd Val)"
                     /label=mKate2 (+2nd Val)
     misc_feature    1495..3369
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    1495..1579
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      2060..3369
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(3390..3410)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(3407..3429)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(3416..3438)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(3778..4460)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(4555..5415)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(4558..5217)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tgaagttcct attccgaagt tcctattctt caaatagtat aggaacttcg
      481 aagatcggcc actacattct ggcctaggag gctgctgcgg ttccagaggt gggaagttcc
      541 tattccgaag ttcctattct ctagaaagta taggaacttc aataaaagtt aatttaagcg
      601 ttcattacaa taaattcaag tgaatctgtt ttaagctagc ctttaacgtt tttaaattcc
      661 ttcaaacaaa ttggatcctc tttgcagttc ctcatttctc ggtgagctgg tgctttaatc
      721 cctttacttt ttcttcctct agggggagga ggaggatctg gaggaggagg atctggagga
      781 ggaggatcta tggtgagcga gctgattaag gagaacatgc acatgaagct gtacatggag
      841 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      901 ggcacccaga ccatgagaat caaggcggtc gagggcggcc ctctcccctt cgccttcgac
      961 atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac ccagggcatc
     1021 cccgacttct ttaagcagtc cttccccgag ggcttcacat gggagagagt caccacatac
     1081 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     1141 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     1201 acactcggct gggaggcctc caccgagacc ctgtaccccg ctgacggcgg cctggaaggc
     1261 agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttgaagacc
     1321 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
     1381 agaagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg
     1441 gctgtggcca gatactgcga cctccctagc aaactggggc acagataaac tagtattaag
     1501 tctcagccac cgttaactga acatgtcaaa acctgtggag actgttgaga tttgatgttc
     1561 tgaaaagata aagcctataa ataaaacaat actcaaatac aaaaggtatc ttttttaaaa
     1621 agttagttta ctgcttcctt aggctctttc taaatcccac ccacctgaag gacatcaatt
     1681 atggatttat ccttcatgcc ttgtataaat acagatgtgc atatgaattt ttatgggttt
     1741 tttaaaaatg tggttcattc tatatacata acaattactt tcaacataca taagcctacc
     1801 tgtatttgtg cattatatta catggtacag ataaatagtc atgtgcccca tgaacatttt
     1861 ggtcaaagat agattacaga tacaatggtg gtcccataag attatgatgc catattttta
     1921 ctgtacctct tcttgtgcct agatacacaa atactgactt gtgttataat tgcctgcagt
     1981 attcaataat atggtgtata gatttgtagc ctacgagcta taacctatac tatatagcca
     2041 aggagtgcag gagggtatgc catctagatt tgtgtgagta cactgatgtt tgaacaataa
     2101 aatcacctaa tgataggttt ctcaaaccat atcccagtcc ttaagcaaca catgactaat
     2161 ggattcaacc atttcccctg ttcgttcaga ttgctttttc tttttgcccc tacaattttg
     2221 ttgataatac tgttttttgg ggcttttgtt tttgtttttt tttttttttt tgagacaaga
     2281 gtctcactct gtcgtcaggc tggagtgcaa tggcgctatc tcagctcact gcaacctctg
     2341 cctcccaggt tcaagcgatt ctcctgcctc agcctcctga gtagctgaga ttacaggcat
     2401 gcgccaccgc gcccggctaa ttttgtattt ttagtagaga cgcagtttca ccatgttggt
     2461 caggctggtc ttgaacaccc gacctcaggt gatccacccg cctcagcttc ccaaagtgct
     2521 gggattaaag gcgtgagcca ccacacctgg ccaatttact gttttatacc atgctttttt
     2581 gtgtgtattc taggttccct aaagcaaaca tgcattttaa attttgatag aaccagatga
     2641 catacaaaag tgcctgtttt ctctgcatta ttaaaactga aggtaatcag tcttttattt
     2701 ctatgactcc aactggcaaa aaatatttgc cattttaact tttttttttt gagacggagt
     2761 caaaaggtgc cagttttctc tgcattatta aaactgaagg taatcagtct tttatttcta
     2821 tgactccaac tggcaaaaga tatttgccac gttaactttt tttttttttt ttttgagacg
     2881 gaggctcaca ctgtcaccca ggctgaagtg cagtggcatg atctcagctc acttgcaacc
     2941 tccgcctccc aggttcaagt gattctcctg cctcagcctc ccaaggagtt gggattacag
     3001 gcgcataccc ggcttatttt ttgtattttt agtagaaacg ggatttcacc gtgttagcca
     3061 ggatggtctc aatctccaga cttcgtgatc gcccacctcg gcctcccaaa gtgctgggat
     3121 tacaggcgtg agccaccgcg cccggccctc agtaggcgat ttttatatgt ggtataaagt
     3181 agaattctta cagatggata cccgtttatg ccagtgccat gactgaaacc ctgcttgtcc
     3241 aacttgaaat atcttcaatg cccatctatt cttccttgat cttgtttttt cctgtgataa
     3301 tacaatagtt tttaccagtt aatcagttaa gatgaggctt accaaatata tgggagtact
     3361 actgaacacg atatcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     3421 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     3481 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3541 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3601 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3661 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3841 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3961 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4021 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4081 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4141 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4201 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4261 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4321 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4381 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4441 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4501 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4561 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4621 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4681 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4741 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4801 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4861 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4921 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4981 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5041 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5101 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5161 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5221 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5281 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5341 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5401 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5461 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5521 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     5581 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
