LOCUS       Exported                5673 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pSA_0_mTagBFP2 from 1 to 4252
            F
            nnncctaggaGGCTGCTGCGGTTCCAGAGGTGGGAAGTTCCTATTCcGAAGTTCCTATTCTCTAGAA 
            AGTATAGGAACTTC
            R:
            GCCcctaggccaGAATGTAGTGGCCGATCTTCGAAGTTCCTATACTATTTGAAGAATAGGAACTTCg 
            GAATAGGAACTTCATCTAGATGCATTCGCGAGGT
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_feature    432..479
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    480..499
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    510..529
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    533..580
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     intron          581..742
                     /locus_tag="B-actin intron 3'"
                     /label=B-actin intron 3'
     variation       743..787
                     /locus_tag="flexible linker (GGGGS)x3 zf-opt"
                     /label=flexible linker (GGGGS)x3 zf-opt
     variation       743..757
                     /locus_tag="(GGGGS)linker"
                     /label=(GGGGS)linker
     exon            743..745
                     /locus_tag="B-actin exon 2 start (SA_0)"
                     /label=B-actin exon 2 start (SA_0)
     variation       758..772
                     /locus_tag="(GGGGS)linker(1)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(1)
     variation       773..787
                     /locus_tag="(GGGGS)linker(2)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(2)
     misc_feature    788..1486
                     /locus_tag="zf-mTagBFP2 (no TAA)"
                     /label=zf-mTagBFP2 (no TAA)
     variation       1487..1501
                     /locus_tag="(GGGGS)linker(3)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(3)
     misc_feature    1502..1543
                     /locus_tag="V5-tag zf-optim (GKPIPNPLLGLDST)"
                     /label=V5-tag zf-optim (GKPIPNPLLGLDST)
     misc_feature    1553..3427
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    1553..1637
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      2118..3427
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(3448..3468)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(3465..3487)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(3474..3496)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(3836..4518)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(4613..5473)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(4616..5275)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tgaagttcct attccgaagt tcctattctt caaatagtat aggaacttcg
      481 aagatcggcc actacattct ggcctaggag gctgctgcgg ttccagaggt gggaagttcc
      541 tattccgaag ttcctattct ctagaaagta taggaacttc aataaaagtt aatttaagcg
      601 ttcattacaa taaattcaag tgaatctgtt ttaagctagc ctttaacgtt tttaaattcc
      661 ttcaaacaaa ttggatcctc tttgcagttc ctcatttctc ggtgagctgg tgctttaatc
      721 cctttacttt ttcttcctct agggaggagg aggatctgga ggaggaggat ctggaggagg
      781 aggatctatg tccgaactta tcaaagaaaa tatgcatatg aagctgtaca tggagggaac
      841 tgtggacaac caccacttca agtgcacctc tgagggagag ggaaagcctt atgagggaac
      901 ccagaccatg cgcatcaagg tggtggaggg aggacctctg ccttttgctt ttgacatcct
      961 ggccacctct ttcctgtatg gaagcaagac cttcatcaac cacacccagg gaatccctga
     1021 cttcttcaag cagtctttcc ctgagggatt cacctgggaa agagtgacca cctacgagga
     1081 tggaggagtg ctgaccgcca cccaggacac ctctctccag gatggatgcc tgatctacaa
     1141 cgtgaagatc agaggagtga actttactag caacggacct gtgatgcaaa agaagaccct
     1201 gggatgggag gctttcactg agaccctgta cccagctgat ggaggactgg agggaagaaa
     1261 tgacatggct ctgaagctgg tgggaggatc tcacctgatc gccaatgcca agaccaccta
     1321 cagaagcaag aagcctgcca agaacctgaa gatgcctgga gtgtactatg tggactacag
     1381 actggagcgc atcaaggagg ccaacaatga gacctatgtg gagcagcatg aggtggctgt
     1441 ggccagatat tgtgacctcc ctagcaagct gggacacaag ctgaatggag gaggaggatc
     1501 tggaaagcca atcccaaacc ctctgctggg actggacagc acctaaacta gtattaagtc
     1561 tcagccaccg ttaactgaac atgtcaaaac ctgtggagac tgttgagatt tgatgttctg
     1621 aaaagataaa gcctataaat aaaacaatac tcaaatacaa aaggtatctt ttttaaaaag
     1681 ttagtttact gcttccttag gctctttcta aatcccaccc acctgaagga catcaattat
     1741 ggatttatcc ttcatgcctt gtataaatac agatgtgcat atgaattttt atgggttttt
     1801 taaaaatgtg gttcattcta tatacataac aattactttc aacatacata agcctacctg
     1861 tatttgtgca ttatattaca tggtacagat aaatagtcat gtgccccatg aacattttgg
     1921 tcaaagatag attacagata caatggtggt cccataagat tatgatgcca tatttttact
     1981 gtacctcttc ttgtgcctag atacacaaat actgacttgt gttataattg cctgcagtat
     2041 tcaataatat ggtgtataga tttgtagcct acgagctata acctatacta tatagccaag
     2101 gagtgcagga gggtatgcca tctagatttg tgtgagtaca ctgatgtttg aacaataaaa
     2161 tcacctaatg ataggtttct caaaccatat cccagtcctt aagcaacaca tgactaatgg
     2221 attcaaccat ttcccctgtt cgttcagatt gctttttctt tttgccccta caattttgtt
     2281 gataatactg ttttttgggg cttttgtttt tgtttttttt tttttttttg agacaagagt
     2341 ctcactctgt cgtcaggctg gagtgcaatg gcgctatctc agctcactgc aacctctgcc
     2401 tcccaggttc aagcgattct cctgcctcag cctcctgagt agctgagatt acaggcatgc
     2461 gccaccgcgc ccggctaatt ttgtattttt agtagagacg cagtttcacc atgttggtca
     2521 ggctggtctt gaacacccga cctcaggtga tccacccgcc tcagcttccc aaagtgctgg
     2581 gattaaaggc gtgagccacc acacctggcc aatttactgt tttataccat gcttttttgt
     2641 gtgtattcta ggttccctaa agcaaacatg cattttaaat tttgatagaa ccagatgaca
     2701 tacaaaagtg cctgttttct ctgcattatt aaaactgaag gtaatcagtc ttttatttct
     2761 atgactccaa ctggcaaaaa atatttgcca ttttaacttt ttttttttga gacggagtca
     2821 aaaggtgcca gttttctctg cattattaaa actgaaggta atcagtcttt tatttctatg
     2881 actccaactg gcaaaagata tttgccacgt taactttttt tttttttttt ttgagacgga
     2941 ggctcacact gtcacccagg ctgaagtgca gtggcatgat ctcagctcac ttgcaacctc
     3001 cgcctcccag gttcaagtga ttctcctgcc tcagcctccc aaggagttgg gattacaggc
     3061 gcatacccgg cttatttttt gtatttttag tagaaacggg atttcaccgt gttagccagg
     3121 atggtctcaa tctccagact tcgtgatcgc ccacctcggc ctcccaaagt gctgggatta
     3181 caggcgtgag ccaccgcgcc cggccctcag taggcgattt ttatatgtgg tataaagtag
     3241 aattcttaca gatggatacc cgtttatgcc agtgccatga ctgaaaccct gcttgtccaa
     3301 cttgaaatat cttcaatgcc catctattct tccttgatct tgttttttcc tgtgataata
     3361 caatagtttt taccagttaa tcagttaaga tgaggcttac caaatatatg ggagtactac
     3421 tgaacacgat atcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3481 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3541 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3601 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3661 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3721 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3781 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3841 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3901 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3961 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4021 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4081 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4141 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4201 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4261 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4321 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4381 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4441 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4501 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4561 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4621 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4681 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4741 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4801 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4861 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4921 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4981 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5041 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5101 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5161 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5221 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5281 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5341 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5401 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5461 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5521 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5581 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     5641 ataaaaatag gcgtatcacg aggccctttc gtc
//
