LOCUS       Exported                5674 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pSA_0_mTagBFP2_synCoTC from 1 to 5673
            pSA_0_mTagBFP2 from 1 to 4252
            F
            nnncctaggaGGCTGCTGCGGTTCCAGAGGTGGGAAGTTCCTATTCcGAAGTTCCTATTCTCTAGAA 
            AGTATAGGAACTTC
            R:
            GCCcctaggccaGAATGTAGTGGCCGATCTTCGAAGTTCCTATACTATTTGAAGAATAGGAACTTCg 
            GAATAGGAACTTCATCTAGATGCATTCGCGAGGT
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_feature    432..479
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    480..499
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    510..529
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    533..580
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     intron          581..742
                     /locus_tag="B-actin intron 3'"
                     /label=B-actin intron 3'
     exon            743..746
                     /locus_tag="B-actin exon 2 start (SA_1)"
                     /label=B-actin exon 2 start (SA_1)
     variation       744..788
                     /locus_tag="flexible linker (GGGGS)x3 zf-opt"
                     /label=flexible linker (GGGGS)x3 zf-opt
     variation       744..758
                     /locus_tag="(GGGGS)linker"
                     /label=(GGGGS)linker
     variation       759..773
                     /locus_tag="(GGGGS)linker(1)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(1)
     variation       774..788
                     /locus_tag="(GGGGS)linker(2)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(2)
     misc_feature    789..1487
                     /locus_tag="zf-mTagBFP2 (no TAA)"
                     /label=zf-mTagBFP2 (no TAA)
     variation       1488..1502
                     /locus_tag="(GGGGS)linker(3)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(3)
     misc_feature    1503..1544
                     /locus_tag="V5-tag zf-optim (GKPIPNPLLGLDST)"
                     /label=V5-tag zf-optim (GKPIPNPLLGLDST)
     misc_feature    1554..3428
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    1554..1638
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      2119..3428
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(3449..3469)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(3466..3488)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(3475..3497)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(3837..4519)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(4614..5474)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(4617..5276)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tgaagttcct attccgaagt tcctattctt caaatagtat aggaacttcg
      481 aagatcggcc actacattct ggcctaggag gctgctgcgg ttccagaggt gggaagttcc
      541 tattccgaag ttcctattct ctagaaagta taggaacttc aataaaagtt aatttaagcg
      601 ttcattacaa taaattcaag tgaatctgtt ttaagctagc ctttaacgtt tttaaattcc
      661 ttcaaacaaa ttggatcctc tttgcagttc ctcatttctc ggtgagctgg tgctttaatc
      721 cctttacttt ttcttcctct aggggaggag gaggatctgg aggaggagga tctggaggag
      781 gaggatctat gtccgaactt atcaaagaaa atatgcatat gaagctgtac atggagggaa
      841 ctgtggacaa ccaccacttc aagtgcacct ctgagggaga gggaaagcct tatgagggaa
      901 cccagaccat gcgcatcaag gtggtggagg gaggacctct gccttttgct tttgacatcc
      961 tggccacctc tttcctgtat ggaagcaaga ccttcatcaa ccacacccag ggaatccctg
     1021 acttcttcaa gcagtctttc cctgagggat tcacctggga aagagtgacc acctacgagg
     1081 atggaggagt gctgaccgcc acccaggaca cctctctcca ggatggatgc ctgatctaca
     1141 acgtgaagat cagaggagtg aactttacta gcaacggacc tgtgatgcaa aagaagaccc
     1201 tgggatggga ggctttcact gagaccctgt acccagctga tggaggactg gagggaagaa
     1261 atgacatggc tctgaagctg gtgggaggat ctcacctgat cgccaatgcc aagaccacct
     1321 acagaagcaa gaagcctgcc aagaacctga agatgcctgg agtgtactat gtggactaca
     1381 gactggagcg catcaaggag gccaacaatg agacctatgt ggagcagcat gaggtggctg
     1441 tggccagata ttgtgacctc cctagcaagc tgggacacaa gctgaatgga ggaggaggat
     1501 ctggaaagcc aatcccaaac cctctgctgg gactggacag cacctaaact agtattaagt
     1561 ctcagccacc gttaactgaa catgtcaaaa cctgtggaga ctgttgagat ttgatgttct
     1621 gaaaagataa agcctataaa taaaacaata ctcaaataca aaaggtatct tttttaaaaa
     1681 gttagtttac tgcttcctta ggctctttct aaatcccacc cacctgaagg acatcaatta
     1741 tggatttatc cttcatgcct tgtataaata cagatgtgca tatgaatttt tatgggtttt
     1801 ttaaaaatgt ggttcattct atatacataa caattacttt caacatacat aagcctacct
     1861 gtatttgtgc attatattac atggtacaga taaatagtca tgtgccccat gaacattttg
     1921 gtcaaagata gattacagat acaatggtgg tcccataaga ttatgatgcc atatttttac
     1981 tgtacctctt cttgtgccta gatacacaaa tactgacttg tgttataatt gcctgcagta
     2041 ttcaataata tggtgtatag atttgtagcc tacgagctat aacctatact atatagccaa
     2101 ggagtgcagg agggtatgcc atctagattt gtgtgagtac actgatgttt gaacaataaa
     2161 atcacctaat gataggtttc tcaaaccata tcccagtcct taagcaacac atgactaatg
     2221 gattcaacca tttcccctgt tcgttcagat tgctttttct ttttgcccct acaattttgt
     2281 tgataatact gttttttggg gcttttgttt ttgttttttt tttttttttt gagacaagag
     2341 tctcactctg tcgtcaggct ggagtgcaat ggcgctatct cagctcactg caacctctgc
     2401 ctcccaggtt caagcgattc tcctgcctca gcctcctgag tagctgagat tacaggcatg
     2461 cgccaccgcg cccggctaat tttgtatttt tagtagagac gcagtttcac catgttggtc
     2521 aggctggtct tgaacacccg acctcaggtg atccacccgc ctcagcttcc caaagtgctg
     2581 ggattaaagg cgtgagccac cacacctggc caatttactg ttttatacca tgcttttttg
     2641 tgtgtattct aggttcccta aagcaaacat gcattttaaa ttttgataga accagatgac
     2701 atacaaaagt gcctgttttc tctgcattat taaaactgaa ggtaatcagt cttttatttc
     2761 tatgactcca actggcaaaa aatatttgcc attttaactt tttttttttg agacggagtc
     2821 aaaaggtgcc agttttctct gcattattaa aactgaaggt aatcagtctt ttatttctat
     2881 gactccaact ggcaaaagat atttgccacg ttaacttttt tttttttttt tttgagacgg
     2941 aggctcacac tgtcacccag gctgaagtgc agtggcatga tctcagctca cttgcaacct
     3001 ccgcctccca ggttcaagtg attctcctgc ctcagcctcc caaggagttg ggattacagg
     3061 cgcatacccg gcttattttt tgtattttta gtagaaacgg gatttcaccg tgttagccag
     3121 gatggtctca atctccagac ttcgtgatcg cccacctcgg cctcccaaag tgctgggatt
     3181 acaggcgtga gccaccgcgc ccggccctca gtaggcgatt tttatatgtg gtataaagta
     3241 gaattcttac agatggatac ccgtttatgc cagtgccatg actgaaaccc tgcttgtcca
     3301 acttgaaata tcttcaatgc ccatctattc ttccttgatc ttgttttttc ctgtgataat
     3361 acaatagttt ttaccagtta atcagttaag atgaggctta ccaaatatat gggagtacta
     3421 ctgaacacga tatcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3481 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3541 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3601 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3661 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3721 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3781 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3841 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3901 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3961 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4021 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4081 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4141 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4201 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4261 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4321 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4381 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4441 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4501 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4561 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4621 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4681 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4741 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4801 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4861 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4921 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4981 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5041 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5101 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5161 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5221 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5281 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5341 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5401 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5461 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5521 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5581 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     5641 tataaaaata ggcgtatcac gaggcccttt cgtc
//
