LOCUS       Exported                5675 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pSA_1_mTagBFP2_synCoTC from 1 to 5674
            pSA_0_mTagBFP2_synCoTC from 1 to 5673
            pSA_0_mTagBFP2 from 1 to 4252
            F
            nnncctaggaGGCTGCTGCGGTTCCAGAGGTGGGAAGTTCCTATTCcGAAGTTCCTATTCTCTAGAA 
            AGTATAGGAACTTC
            R:
            GCCcctaggccaGAATGTAGTGGCCGATCTTCGAAGTTCCTATACTATTTGAAGAATAGGAACTTCg 
            GAATAGGAACTTCATCTAGATGCATTCGCGAGGT
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_feature    432..479
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    480..499
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    510..529
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    533..580
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     intron          581..742
                     /locus_tag="B-actin intron 3'"
                     /label=B-actin intron 3'
     exon            743..747
                     /locus_tag="B-actin exon 2 start (SA_2)"
                     /label=B-actin exon 2 start (SA_2)
     variation       745..789
                     /locus_tag="flexible linker (GGGGS)x3 zf-opt"
                     /label=flexible linker (GGGGS)x3 zf-opt
     variation       745..759
                     /locus_tag="(GGGGS)linker"
                     /label=(GGGGS)linker
     variation       760..774
                     /locus_tag="(GGGGS)linker(1)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(1)
     variation       775..789
                     /locus_tag="(GGGGS)linker(2)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(2)
     misc_feature    790..1488
                     /locus_tag="zf-mTagBFP2 (no TAA)"
                     /label=zf-mTagBFP2 (no TAA)
     variation       1489..1503
                     /locus_tag="(GGGGS)linker(3)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(3)
     misc_feature    1504..1545
                     /locus_tag="V5-tag zf-optim (GKPIPNPLLGLDST)"
                     /label=V5-tag zf-optim (GKPIPNPLLGLDST)
     misc_feature    1555..3429
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    1555..1639
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      2120..3429
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(3450..3470)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(3467..3489)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(3476..3498)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(3838..4520)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(4615..5475)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(4618..5277)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tgaagttcct attccgaagt tcctattctt caaatagtat aggaacttcg
      481 aagatcggcc actacattct ggcctaggag gctgctgcgg ttccagaggt gggaagttcc
      541 tattccgaag ttcctattct ctagaaagta taggaacttc aataaaagtt aatttaagcg
      601 ttcattacaa taaattcaag tgaatctgtt ttaagctagc ctttaacgtt tttaaattcc
      661 ttcaaacaaa ttggatcctc tttgcagttc ctcatttctc ggtgagctgg tgctttaatc
      721 cctttacttt ttcttcctct agggggagga ggaggatctg gaggaggagg atctggagga
      781 ggaggatcta tgtccgaact tatcaaagaa aatatgcata tgaagctgta catggaggga
      841 actgtggaca accaccactt caagtgcacc tctgagggag agggaaagcc ttatgaggga
      901 acccagacca tgcgcatcaa ggtggtggag ggaggacctc tgccttttgc ttttgacatc
      961 ctggccacct ctttcctgta tggaagcaag accttcatca accacaccca gggaatccct
     1021 gacttcttca agcagtcttt ccctgaggga ttcacctggg aaagagtgac cacctacgag
     1081 gatggaggag tgctgaccgc cacccaggac acctctctcc aggatggatg cctgatctac
     1141 aacgtgaaga tcagaggagt gaactttact agcaacggac ctgtgatgca aaagaagacc
     1201 ctgggatggg aggctttcac tgagaccctg tacccagctg atggaggact ggagggaaga
     1261 aatgacatgg ctctgaagct ggtgggagga tctcacctga tcgccaatgc caagaccacc
     1321 tacagaagca agaagcctgc caagaacctg aagatgcctg gagtgtacta tgtggactac
     1381 agactggagc gcatcaagga ggccaacaat gagacctatg tggagcagca tgaggtggct
     1441 gtggccagat attgtgacct ccctagcaag ctgggacaca agctgaatgg aggaggagga
     1501 tctggaaagc caatcccaaa ccctctgctg ggactggaca gcacctaaac tagtattaag
     1561 tctcagccac cgttaactga acatgtcaaa acctgtggag actgttgaga tttgatgttc
     1621 tgaaaagata aagcctataa ataaaacaat actcaaatac aaaaggtatc ttttttaaaa
     1681 agttagttta ctgcttcctt aggctctttc taaatcccac ccacctgaag gacatcaatt
     1741 atggatttat ccttcatgcc ttgtataaat acagatgtgc atatgaattt ttatgggttt
     1801 tttaaaaatg tggttcattc tatatacata acaattactt tcaacataca taagcctacc
     1861 tgtatttgtg cattatatta catggtacag ataaatagtc atgtgcccca tgaacatttt
     1921 ggtcaaagat agattacaga tacaatggtg gtcccataag attatgatgc catattttta
     1981 ctgtacctct tcttgtgcct agatacacaa atactgactt gtgttataat tgcctgcagt
     2041 attcaataat atggtgtata gatttgtagc ctacgagcta taacctatac tatatagcca
     2101 aggagtgcag gagggtatgc catctagatt tgtgtgagta cactgatgtt tgaacaataa
     2161 aatcacctaa tgataggttt ctcaaaccat atcccagtcc ttaagcaaca catgactaat
     2221 ggattcaacc atttcccctg ttcgttcaga ttgctttttc tttttgcccc tacaattttg
     2281 ttgataatac tgttttttgg ggcttttgtt tttgtttttt tttttttttt tgagacaaga
     2341 gtctcactct gtcgtcaggc tggagtgcaa tggcgctatc tcagctcact gcaacctctg
     2401 cctcccaggt tcaagcgatt ctcctgcctc agcctcctga gtagctgaga ttacaggcat
     2461 gcgccaccgc gcccggctaa ttttgtattt ttagtagaga cgcagtttca ccatgttggt
     2521 caggctggtc ttgaacaccc gacctcaggt gatccacccg cctcagcttc ccaaagtgct
     2581 gggattaaag gcgtgagcca ccacacctgg ccaatttact gttttatacc atgctttttt
     2641 gtgtgtattc taggttccct aaagcaaaca tgcattttaa attttgatag aaccagatga
     2701 catacaaaag tgcctgtttt ctctgcatta ttaaaactga aggtaatcag tcttttattt
     2761 ctatgactcc aactggcaaa aaatatttgc cattttaact tttttttttt gagacggagt
     2821 caaaaggtgc cagttttctc tgcattatta aaactgaagg taatcagtct tttatttcta
     2881 tgactccaac tggcaaaaga tatttgccac gttaactttt tttttttttt ttttgagacg
     2941 gaggctcaca ctgtcaccca ggctgaagtg cagtggcatg atctcagctc acttgcaacc
     3001 tccgcctccc aggttcaagt gattctcctg cctcagcctc ccaaggagtt gggattacag
     3061 gcgcataccc ggcttatttt ttgtattttt agtagaaacg ggatttcacc gtgttagcca
     3121 ggatggtctc aatctccaga cttcgtgatc gcccacctcg gcctcccaaa gtgctgggat
     3181 tacaggcgtg agccaccgcg cccggccctc agtaggcgat ttttatatgt ggtataaagt
     3241 agaattctta cagatggata cccgtttatg ccagtgccat gactgaaacc ctgcttgtcc
     3301 aacttgaaat atcttcaatg cccatctatt cttccttgat cttgtttttt cctgtgataa
     3361 tacaatagtt tttaccagtt aatcagttaa gatgaggctt accaaatata tgggagtact
     3421 actgaacacg atatcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     3481 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     3541 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3601 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3661 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3721 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3781 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3841 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3901 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3961 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4021 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4081 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4141 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4201 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4261 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4321 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4381 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4441 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4501 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4561 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4621 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4681 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4741 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4801 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4861 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4921 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4981 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5041 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5101 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5161 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5221 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5281 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5341 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5401 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5461 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5521 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5581 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     5641 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
