LOCUS       Exported                6153 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     pSA_0_mTagBFP2_synCoTC from 1 to 5673
            pSA_0_mTagBFP2 from 1 to 4252
            F
            nnncctaggaGGCTGCTGCGGTTCCAGAGGTGGGAAGTTCCTATTCcGAAGTTCCTATTCTCTAGAA 
            AGTATAGGAACTTC
            R:
            GCCcctaggccaGAATGTAGTGGCCGATCTTCGAAGTTCCTATACTATTTGAAGAATAGGAACTTCg 
            GAATAGGAACTTCATCTAGATGCATTCGCGAGGT
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     misc_feature    432..479
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    480..499
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    510..529
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    533..580
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     intron          581..742
                     /locus_tag="B-actin intron 3'"
                     /label=B-actin intron 3'
     variation       743..787
                     /locus_tag="flexible linker (GGGGS)x3 zf-opt"
                     /label=flexible linker (GGGGS)x3 zf-opt
     variation       743..757
                     /locus_tag="(GGGGS)linker"
                     /label=(GGGGS)linker
     exon            743..745
                     /locus_tag="B-actin exon 2 start (SA_0)"
                     /label=B-actin exon 2 start (SA_0)
     variation       758..772
                     /locus_tag="(GGGGS)linker(1)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(1)
     variation       773..787
                     /locus_tag="(GGGGS)linker(2)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(2)
     misc_feature    788..1486
                     /locus_tag="zf-mTagBFP2 (no TAA)"
                     /label=zf-mTagBFP2 (no TAA)
     variation       1487..1501
                     /locus_tag="(GGGGS)linker(3)"
                     /label=(GGGGS)linker
                     /label=(GGGGS)linker(3)
     misc_feature    1502..1543
                     /locus_tag="V5-tag zf-optim (GKPIPNPLLGLDST)"
                     /label=V5-tag zf-optim (GKPIPNPLLGLDST)
     protein_bind    1544..1597
                     /locus_tag="zf_opt-T2A"
                     /label=zf_opt-T2A
     misc_feature    1598..2026
                     /locus_tag="zf-sGFP11x7 (5aa linker)"
                     /label=zf-sGFP11x7 (5aa linker)
     misc_binding    1598..1645
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
     misc_binding    1661..1708
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(1)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(1)
     misc_binding    1724..1771
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(2)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(2)
     misc_binding    1787..1834
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(3)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(3)
     misc_binding    1850..1897
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(4)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(4)
     misc_binding    1913..1960
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(5)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(5)
     misc_binding    1976..2023
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(6)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(6)
     misc_feature    2033..3907
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    2033..2117
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      2598..3907
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(3928..3948)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(3945..3967)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(3954..3976)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(4316..4998)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(5093..5953)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(5096..5755)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tgaagttcct attccgaagt tcctattctt caaatagtat aggaacttcg
      481 aagatcggcc actacattct ggcctaggag gctgctgcgg ttccagaggt gggaagttcc
      541 tattccgaag ttcctattct ctagaaagta taggaacttc aataaaagtt aatttaagcg
      601 ttcattacaa taaattcaag tgaatctgtt ttaagctagc ctttaacgtt tttaaattcc
      661 ttcaaacaaa ttggatcctc tttgcagttc ctcatttctc ggtgagctgg tgctttaatc
      721 cctttacttt ttcttcctct agggaggagg aggatctgga ggaggaggat ctggaggagg
      781 aggatctatg tccgaactta tcaaagaaaa tatgcatatg aagctgtaca tggagggaac
      841 tgtggacaac caccacttca agtgcacctc tgagggagag ggaaagcctt atgagggaac
      901 ccagaccatg cgcatcaagg tggtggaggg aggacctctg ccttttgctt ttgacatcct
      961 ggccacctct ttcctgtatg gaagcaagac cttcatcaac cacacccagg gaatccctga
     1021 cttcttcaag cagtctttcc ctgagggatt cacctgggaa agagtgacca cctacgagga
     1081 tggaggagtg ctgaccgcca cccaggacac ctctctccag gatggatgcc tgatctacaa
     1141 cgtgaagatc agaggagtga actttactag caacggacct gtgatgcaaa agaagaccct
     1201 gggatgggag gctttcactg agaccctgta cccagctgat ggaggactgg agggaagaaa
     1261 tgacatggct ctgaagctgg tgggaggatc tcacctgatc gccaatgcca agaccaccta
     1321 cagaagcaag aagcctgcca agaacctgaa gatgcctgga gtgtactatg tggactacag
     1381 actggagcgc atcaaggagg ccaacaatga gacctatgtg gagcagcatg aggtggctgt
     1441 ggccagatat tgtgacctcc ctagcaagct gggacacaag ctgaatggag gaggaggatc
     1501 tggaaagcca atcccaaacc ctctgctggg actggacagc accgagggaa gaggatctct
     1561 cctgacctgt ggagatgtgg aggagaaccc tggcccaaga gaccacatgg tcctgcatga
     1621 gtacgtgaac gccgctggaa ttactggagg atctggagga agagaccaca tggtcctgca
     1681 tgagtacgtg aacgccgctg gaattactgg aggatctgga ggaagagacc acatggtcct
     1741 gcatgagtac gtgaacgccg ctggaattac tggaggatct ggaggaagag accacatggt
     1801 cctgcatgag tacgtgaacg ccgctggaat tactggagga tctggaggaa gagaccacat
     1861 ggtcctgcat gagtacgtga acgccgctgg aattactgga ggatctggag gaagagacca
     1921 catggtcctg catgagtacg tgaacgccgc tggaattact ggaggatctg gaggaagaga
     1981 ccacatggtc ctgcatgagt acgtgaacgc cgctggaatt acttaaacta gtattaagtc
     2041 tcagccaccg ttaactgaac atgtcaaaac ctgtggagac tgttgagatt tgatgttctg
     2101 aaaagataaa gcctataaat aaaacaatac tcaaatacaa aaggtatctt ttttaaaaag
     2161 ttagtttact gcttccttag gctctttcta aatcccaccc acctgaagga catcaattat
     2221 ggatttatcc ttcatgcctt gtataaatac agatgtgcat atgaattttt atgggttttt
     2281 taaaaatgtg gttcattcta tatacataac aattactttc aacatacata agcctacctg
     2341 tatttgtgca ttatattaca tggtacagat aaatagtcat gtgccccatg aacattttgg
     2401 tcaaagatag attacagata caatggtggt cccataagat tatgatgcca tatttttact
     2461 gtacctcttc ttgtgcctag atacacaaat actgacttgt gttataattg cctgcagtat
     2521 tcaataatat ggtgtataga tttgtagcct acgagctata acctatacta tatagccaag
     2581 gagtgcagga gggtatgcca tctagatttg tgtgagtaca ctgatgtttg aacaataaaa
     2641 tcacctaatg ataggtttct caaaccatat cccagtcctt aagcaacaca tgactaatgg
     2701 attcaaccat ttcccctgtt cgttcagatt gctttttctt tttgccccta caattttgtt
     2761 gataatactg ttttttgggg cttttgtttt tgtttttttt tttttttttg agacaagagt
     2821 ctcactctgt cgtcaggctg gagtgcaatg gcgctatctc agctcactgc aacctctgcc
     2881 tcccaggttc aagcgattct cctgcctcag cctcctgagt agctgagatt acaggcatgc
     2941 gccaccgcgc ccggctaatt ttgtattttt agtagagacg cagtttcacc atgttggtca
     3001 ggctggtctt gaacacccga cctcaggtga tccacccgcc tcagcttccc aaagtgctgg
     3061 gattaaaggc gtgagccacc acacctggcc aatttactgt tttataccat gcttttttgt
     3121 gtgtattcta ggttccctaa agcaaacatg cattttaaat tttgatagaa ccagatgaca
     3181 tacaaaagtg cctgttttct ctgcattatt aaaactgaag gtaatcagtc ttttatttct
     3241 atgactccaa ctggcaaaaa atatttgcca ttttaacttt ttttttttga gacggagtca
     3301 aaaggtgcca gttttctctg cattattaaa actgaaggta atcagtcttt tatttctatg
     3361 actccaactg gcaaaagata tttgccacgt taactttttt tttttttttt ttgagacgga
     3421 ggctcacact gtcacccagg ctgaagtgca gtggcatgat ctcagctcac ttgcaacctc
     3481 cgcctcccag gttcaagtga ttctcctgcc tcagcctccc aaggagttgg gattacaggc
     3541 gcatacccgg cttatttttt gtatttttag tagaaacggg atttcaccgt gttagccagg
     3601 atggtctcaa tctccagact tcgtgatcgc ccacctcggc ctcccaaagt gctgggatta
     3661 caggcgtgag ccaccgcgcc cggccctcag taggcgattt ttatatgtgg tataaagtag
     3721 aattcttaca gatggatacc cgtttatgcc agtgccatga ctgaaaccct gcttgtccaa
     3781 cttgaaatat cttcaatgcc catctattct tccttgatct tgttttttcc tgtgataata
     3841 caatagtttt taccagttaa tcagttaaga tgaggcttac caaatatatg ggagtactac
     3901 tgaacacgat atcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4021 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4081 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4141 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4201 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4261 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4501 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4741 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4861 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4921 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4981 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5041 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5101 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5161 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5221 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5281 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5341 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5401 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5461 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5521 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5581 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5641 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5701 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5761 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5821 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5881 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5941 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6001 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6061 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     6121 ataaaaatag gcgtatcacg aggccctttc gtc
//
