LOCUS       Exported                7426 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SA_2_Gal4_noPEST_synCoTC from 1 to 7427
            SA_0_Gal4_noPEST_synCoTC from 1 to 7425
            SA_0_Gal4_synCoTC from 1 to 7559
            pUC57-synCoTC from 1 to 4554
            PUC57 from 1 to 2710
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     promoter        414..529
                     /locus_tag="4xnrUAS (Halpern)"
                     /label=4xnrUAS (Halpern)
     promoter        495..511
                     /locus_tag="UAS sequence"
                     /label=UAS sequence
     misc_feature    530..594
                     /locus_tag="E1B"
                     /label=E1B
     misc_feature    557..586
                     /locus_tag="carp beta-actin transcriptional start"
                     /label=carp beta-actin transcriptional start
     5'UTR           607..1295
                     /locus_tag="UBC intron (+1a)"
                     /label=UBC intron (+1a)
     misc_feature    1300..1308
                     /locus_tag="Kozak"
                     /label=Kozak
     misc_feature    join(1303,1306..1309)
                     /locus_tag="Vertebrate Kozak"
                     /label=Vertebrate Kozak
     misc_feature    1306..2010
                     /locus_tag="zf_mTagBFP2 (+2ndVal)"
                     /label=zf_mTagBFP2 (+2ndVal)
     misc_feature    1306..2007
                     /locus_tag="zf_mTagBFP2 (+2ndVal) noTAA"
                     /label=zf_mTagBFP2 (+2ndVal) noTAA
     polyA_signal    2022..2213
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     misc_feature    2229..2276
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    2277..2296
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    2307..2326
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    2330..2377
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     intron          2378..2539
                     /locus_tag="B-actin intron 3'"
                     /label=B-actin intron 3'
     exon            2540..2543
                     /locus_tag="B-actin exon 2 start (SA_1)"
                     /label=B-actin exon 2 start (SA_1)
     misc_feature    2544..2585
                     /locus_tag="V5-tag zf-optim (GKPIPNPLLGLDST)"
                     /label=V5-tag zf-optim (GKPIPNPLLGLDST)
     protein_bind    2592..2645
                     /locus_tag="zf_opt-T2A"
                     /label=zf_opt-T2A
     misc_feature    2646..3299
                     /locus_tag="zf.Gal4-VP16"
                     /label=zf.Gal4-VP16
     protein_bind    2646..3083
                     /locus_tag="zf.Gal4(DBD)"
                     /label=zf.Gal4(DBD)
     protein_bind    3123..3296
                     /locus_tag="zf.VP16 activation domain"
                     /label=zf.VP16 activation domain
     misc_feature    3306..5180
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    3306..3390
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      3871..5180
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(5201..5221)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(5218..5240)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(5227..5249)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(5589..6271)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(6366..7226)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(6369..7028)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggtggc
      421 ttctaatccg tgagtcctag cgggtgacag ccctccgtct tcacaggcgg aggagagtct
      481 tccgtagagt tcctcggagt actgtcctcc gacgcgtgca agggtcgact ctagagggta
      541 tataatggat cccatcgcgt ctcagcctca ctttgagctc ctccacacga attcgatact
      601 aaaagcttgt ttgtgagtat gatgttaaat ataagcaatc gacgtttgtt ttgtcgtttg
      661 ttaaatctag caatgtttta tttcaattaa agtgttaacc ggttcattta aaggtgtatt
      721 tctgaatgag ctaacgtatt agcttttaac gctagccgga ctgtttggtt ttgaggatca
      781 ataaaccaac gtttttttct gatttatttt gctttaaaat ataaaggtat aattcgtcgt
      841 cgagaatttt gggttttgtt cgagtttaga aatttggcgg cgtttttttg taccgcgaaa
      901 cagggcggtg gttcggtgag cttctcaatt catctgcttg acaaacggtt ttcggcaact
      961 gtaaatgcgc tatgatgctt ttattctttt cagcgtgtaa ctttgttaaa tataattgac
     1021 tttccgacta tcgacgttcg tttcatctcc gtgttattgt ttaatggcca ttttctgtgg
     1081 taaactgtcg ccattttgtt ttcagtcgtc cgtttgttcc gcccttactc acagctgtgt
     1141 cagcattagt gatgactgtt ctcacctggc ctgtttgctt tcattattga tgaagagctt
     1201 tctagagaat tctgaagctc gtctttgtcc tgaattcatc tgtattcatt taaaaaaaaa
     1261 aattaacctt aatttttttt tcttctcagc gtaaagcttg ccaccatggt gtccgaactt
     1321 atcaaagaaa atatgcatat gaagctgtac atggagggaa ctgtggacaa ccaccacttc
     1381 aagtgcacct ctgagggaga gggaaagcct tatgagggaa cccagaccat gcgcatcaag
     1441 gtggtggagg gaggacctct gccttttgct tttgacatcc tggccacctc tttcctgtat
     1501 ggaagcaaga ccttcatcaa ccacacccag ggaatccctg acttcttcaa gcagtctttc
     1561 cctgagggat tcacctggga aagagtgacc acctacgagg atggaggagt gctgaccgcc
     1621 acccaggaca cctctctcca ggatggatgc ctgatctaca acgtgaagat cagaggagtg
     1681 aactttacta gcaacggacc tgtgatgcaa aagaagaccc tgggatggga ggctttcact
     1741 gagaccctgt acccagctga tggaggactg gagggaagaa atgacatggc tctgaagctg
     1801 gtgggaggat ctcacctgat cgccaatgcc aagaccacct acagaagcaa gaagcctgcc
     1861 aagaacctga agatgcctgg agtgtactat gtggactaca gactggagcg catcaaggag
     1921 gccaacaatg agacctatgt ggagcagcat gaggtggctg tggccagata ttgtgacctc
     1981 cctagcaagc tgggacacaa gctgaattaa atacgtagat ccagacatga taagatacat
     2041 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2101 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2161 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcgaatgc
     2221 atctagatga agttcctatt ccgaagttcc tattcttcaa atagtatagg aacttcgaag
     2281 atcggccact acattctggc ctaggaggct gctgcggttc cagaggtggg aagttcctat
     2341 tccgaagttc ctattctcta gaaagtatag gaacttcaat aaaagttaat ttaagcgttc
     2401 attacaataa attcaagtga atctgtttta agctagcctt taacgttttt aaattccttc
     2461 aaacaaattg gatcctcttt gcagttcctc atttctcggt gagctggtgc tttaatccct
     2521 ttactttttc ttcctctagg ggaggaaagc caatcccaaa ccctctgctg ggactggaca
     2581 gcaccgacgt cgagggaaga ggatctctcc tgacctgtgg agatgtggag gagaaccctg
     2641 gcccaatgaa acttctatct tctatcgaac aggcttgcga catctgccgc ctgaagaagc
     2701 tgaagtgcag caaggagaag ccaaagtgcg ccaagtgcct gaagaacaac tgggagtgca
     2761 gatactctcc taagaccaag cgctctcctc tgacccgcgc tcacctcact gaggtggaga
     2821 gcagactgga gagactggag cagctgtttc tcctgatctt cccaagagag gacctggaca
     2881 tgatcctgaa gatggactct ctccaggaca tcaaggctct cctgactggc ctgtttgtgc
     2941 aggacaacgt gaacaaggat gctgtgaccg acagactggc ttctgtggag accgacatgc
     3001 cactgactct gagacagcac agaatctctg ccacctcttc ttctgaggag tctagcaaca
     3061 agggacagag acagctgact gtgtctagca gaagcacccc tggaatccag atcagcagag
     3121 ctgctcctcc aactgatgtg tctctcggag atgagctcca cctggatgga gaggatgtgg
     3181 ctatggctca tgctgatgct ctggatgact ttgacctgga catgctcgga gatggagact
     3241 ctccaggacc tggattcacc cctcatgact ctgctcctta tggcgctctg gacatgtaaa
     3301 ctagtattaa gtctcagcca ccgttaactg aacatgtcaa aacctgtgga gactgttgag
     3361 atttgatgtt ctgaaaagat aaagcctata aataaaacaa tactcaaata caaaaggtat
     3421 cttttttaaa aagttagttt actgcttcct taggctcttt ctaaatccca cccacctgaa
     3481 ggacatcaat tatggattta tccttcatgc cttgtataaa tacagatgtg catatgaatt
     3541 tttatgggtt ttttaaaaat gtggttcatt ctatatacat aacaattact ttcaacatac
     3601 ataagcctac ctgtatttgt gcattatatt acatggtaca gataaatagt catgtgcccc
     3661 atgaacattt tggtcaaaga tagattacag atacaatggt ggtcccataa gattatgatg
     3721 ccatattttt actgtacctc ttcttgtgcc tagatacaca aatactgact tgtgttataa
     3781 ttgcctgcag tattcaataa tatggtgtat agatttgtag cctacgagct ataacctata
     3841 ctatatagcc aaggagtgca ggagggtatg ccatctagat ttgtgtgagt acactgatgt
     3901 ttgaacaata aaatcaccta atgataggtt tctcaaacca tatcccagtc cttaagcaac
     3961 acatgactaa tggattcaac catttcccct gttcgttcag attgcttttt ctttttgccc
     4021 ctacaatttt gttgataata ctgttttttg gggcttttgt ttttgttttt tttttttttt
     4081 ttgagacaag agtctcactc tgtcgtcagg ctggagtgca atggcgctat ctcagctcac
     4141 tgcaacctct gcctcccagg ttcaagcgat tctcctgcct cagcctcctg agtagctgag
     4201 attacaggca tgcgccaccg cgcccggcta attttgtatt tttagtagag acgcagtttc
     4261 accatgttgg tcaggctggt cttgaacacc cgacctcagg tgatccaccc gcctcagctt
     4321 cccaaagtgc tgggattaaa ggcgtgagcc accacacctg gccaatttac tgttttatac
     4381 catgcttttt tgtgtgtatt ctaggttccc taaagcaaac atgcatttta aattttgata
     4441 gaaccagatg acatacaaaa gtgcctgttt tctctgcatt attaaaactg aaggtaatca
     4501 gtcttttatt tctatgactc caactggcaa aaaatatttg ccattttaac tttttttttt
     4561 tgagacggag tcaaaaggtg ccagttttct ctgcattatt aaaactgaag gtaatcagtc
     4621 ttttatttct atgactccaa ctggcaaaag atatttgcca cgttaacttt tttttttttt
     4681 tttttgagac ggaggctcac actgtcaccc aggctgaagt gcagtggcat gatctcagct
     4741 cacttgcaac ctccgcctcc caggttcaag tgattctcct gcctcagcct cccaaggagt
     4801 tgggattaca ggcgcatacc cggcttattt tttgtatttt tagtagaaac gggatttcac
     4861 cgtgttagcc aggatggtct caatctccag acttcgtgat cgcccacctc ggcctcccaa
     4921 agtgctggga ttacaggcgt gagccaccgc gcccggccct cagtaggcga tttttatatg
     4981 tggtataaag tagaattctt acagatggat acccgtttat gccagtgcca tgactgaaac
     5041 cctgcttgtc caacttgaaa tatcttcaat gcccatctat tcttccttga tcttgttttt
     5101 tcctgtgata atacaatagt ttttaccagt taatcagtta agatgaggct taccaaatat
     5161 atgggagtac tactgaacac gatatcaagc ttggcgtaat catggtcata gctgtttcct
     5221 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5281 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5341 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6901 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7141 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7201 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7261 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7321 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7381 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
