LOCUS       Exported                7427 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SA_0_Gal4_noPEST_synCoTC from 1 to 7425
            SA_0_Gal4_synCoTC from 1 to 7559
            pUC57-synCoTC from 1 to 4554
            PUC57 from 1 to 2710
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     promoter        414..529
                     /locus_tag="4xnrUAS (Halpern)"
                     /label=4xnrUAS (Halpern)
     promoter        495..511
                     /locus_tag="UAS sequence"
                     /label=UAS sequence
     misc_feature    530..594
                     /locus_tag="E1B"
                     /label=E1B
     misc_feature    557..586
                     /locus_tag="carp beta-actin transcriptional start"
                     /label=carp beta-actin transcriptional start
     5'UTR           607..1295
                     /locus_tag="UBC intron (+1a)"
                     /label=UBC intron (+1a)
     misc_feature    1300..1308
                     /locus_tag="Kozak"
                     /label=Kozak
     misc_feature    join(1303,1306..1309)
                     /locus_tag="Vertebrate Kozak"
                     /label=Vertebrate Kozak
     misc_feature    1306..2010
                     /locus_tag="zf_mTagBFP2 (+2ndVal)"
                     /label=zf_mTagBFP2 (+2ndVal)
     misc_feature    1306..2007
                     /locus_tag="zf_mTagBFP2 (+2ndVal) noTAA"
                     /label=zf_mTagBFP2 (+2ndVal) noTAA
     polyA_signal    2022..2213
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     misc_feature    2229..2276
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    2277..2296
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    2307..2326
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    2330..2377
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     intron          2378..2539
                     /locus_tag="B-actin intron 3'"
                     /label=B-actin intron 3'
     exon            2540..2544
                     /locus_tag="B-actin exon 2 start (SA_2)"
                     /label=B-actin exon 2 start (SA_2)
     misc_feature    2545..2586
                     /locus_tag="V5-tag zf-optim (GKPIPNPLLGLDST)"
                     /label=V5-tag zf-optim (GKPIPNPLLGLDST)
     protein_bind    2593..2646
                     /locus_tag="zf_opt-T2A"
                     /label=zf_opt-T2A
     misc_feature    2647..3300
                     /locus_tag="zf.Gal4-VP16"
                     /label=zf.Gal4-VP16
     protein_bind    2647..3084
                     /locus_tag="zf.Gal4(DBD)"
                     /label=zf.Gal4(DBD)
     protein_bind    3124..3297
                     /locus_tag="zf.VP16 activation domain"
                     /label=zf.VP16 activation domain
     misc_feature    3307..5181
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    3307..3391
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      3872..5181
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(5202..5222)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(5219..5241)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(5228..5250)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(5590..6272)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(6367..7227)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(6370..7029)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggtggc
      421 ttctaatccg tgagtcctag cgggtgacag ccctccgtct tcacaggcgg aggagagtct
      481 tccgtagagt tcctcggagt actgtcctcc gacgcgtgca agggtcgact ctagagggta
      541 tataatggat cccatcgcgt ctcagcctca ctttgagctc ctccacacga attcgatact
      601 aaaagcttgt ttgtgagtat gatgttaaat ataagcaatc gacgtttgtt ttgtcgtttg
      661 ttaaatctag caatgtttta tttcaattaa agtgttaacc ggttcattta aaggtgtatt
      721 tctgaatgag ctaacgtatt agcttttaac gctagccgga ctgtttggtt ttgaggatca
      781 ataaaccaac gtttttttct gatttatttt gctttaaaat ataaaggtat aattcgtcgt
      841 cgagaatttt gggttttgtt cgagtttaga aatttggcgg cgtttttttg taccgcgaaa
      901 cagggcggtg gttcggtgag cttctcaatt catctgcttg acaaacggtt ttcggcaact
      961 gtaaatgcgc tatgatgctt ttattctttt cagcgtgtaa ctttgttaaa tataattgac
     1021 tttccgacta tcgacgttcg tttcatctcc gtgttattgt ttaatggcca ttttctgtgg
     1081 taaactgtcg ccattttgtt ttcagtcgtc cgtttgttcc gcccttactc acagctgtgt
     1141 cagcattagt gatgactgtt ctcacctggc ctgtttgctt tcattattga tgaagagctt
     1201 tctagagaat tctgaagctc gtctttgtcc tgaattcatc tgtattcatt taaaaaaaaa
     1261 aattaacctt aatttttttt tcttctcagc gtaaagcttg ccaccatggt gtccgaactt
     1321 atcaaagaaa atatgcatat gaagctgtac atggagggaa ctgtggacaa ccaccacttc
     1381 aagtgcacct ctgagggaga gggaaagcct tatgagggaa cccagaccat gcgcatcaag
     1441 gtggtggagg gaggacctct gccttttgct tttgacatcc tggccacctc tttcctgtat
     1501 ggaagcaaga ccttcatcaa ccacacccag ggaatccctg acttcttcaa gcagtctttc
     1561 cctgagggat tcacctggga aagagtgacc acctacgagg atggaggagt gctgaccgcc
     1621 acccaggaca cctctctcca ggatggatgc ctgatctaca acgtgaagat cagaggagtg
     1681 aactttacta gcaacggacc tgtgatgcaa aagaagaccc tgggatggga ggctttcact
     1741 gagaccctgt acccagctga tggaggactg gagggaagaa atgacatggc tctgaagctg
     1801 gtgggaggat ctcacctgat cgccaatgcc aagaccacct acagaagcaa gaagcctgcc
     1861 aagaacctga agatgcctgg agtgtactat gtggactaca gactggagcg catcaaggag
     1921 gccaacaatg agacctatgt ggagcagcat gaggtggctg tggccagata ttgtgacctc
     1981 cctagcaagc tgggacacaa gctgaattaa atacgtagat ccagacatga taagatacat
     2041 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2101 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2161 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcgaatgc
     2221 atctagatga agttcctatt ccgaagttcc tattcttcaa atagtatagg aacttcgaag
     2281 atcggccact acattctggc ctaggaggct gctgcggttc cagaggtggg aagttcctat
     2341 tccgaagttc ctattctcta gaaagtatag gaacttcaat aaaagttaat ttaagcgttc
     2401 attacaataa attcaagtga atctgtttta agctagcctt taacgttttt aaattccttc
     2461 aaacaaattg gatcctcttt gcagttcctc atttctcggt gagctggtgc tttaatccct
     2521 ttactttttc ttcctctagg gggaggaaag ccaatcccaa accctctgct gggactggac
     2581 agcaccgacg tcgagggaag aggatctctc ctgacctgtg gagatgtgga ggagaaccct
     2641 ggcccaatga aacttctatc ttctatcgaa caggcttgcg acatctgccg cctgaagaag
     2701 ctgaagtgca gcaaggagaa gccaaagtgc gccaagtgcc tgaagaacaa ctgggagtgc
     2761 agatactctc ctaagaccaa gcgctctcct ctgacccgcg ctcacctcac tgaggtggag
     2821 agcagactgg agagactgga gcagctgttt ctcctgatct tcccaagaga ggacctggac
     2881 atgatcctga agatggactc tctccaggac atcaaggctc tcctgactgg cctgtttgtg
     2941 caggacaacg tgaacaagga tgctgtgacc gacagactgg cttctgtgga gaccgacatg
     3001 ccactgactc tgagacagca cagaatctct gccacctctt cttctgagga gtctagcaac
     3061 aagggacaga gacagctgac tgtgtctagc agaagcaccc ctggaatcca gatcagcaga
     3121 gctgctcctc caactgatgt gtctctcgga gatgagctcc acctggatgg agaggatgtg
     3181 gctatggctc atgctgatgc tctggatgac tttgacctgg acatgctcgg agatggagac
     3241 tctccaggac ctggattcac ccctcatgac tctgctcctt atggcgctct ggacatgtaa
     3301 actagtatta agtctcagcc accgttaact gaacatgtca aaacctgtgg agactgttga
     3361 gatttgatgt tctgaaaaga taaagcctat aaataaaaca atactcaaat acaaaaggta
     3421 tcttttttaa aaagttagtt tactgcttcc ttaggctctt tctaaatccc acccacctga
     3481 aggacatcaa ttatggattt atccttcatg ccttgtataa atacagatgt gcatatgaat
     3541 ttttatgggt tttttaaaaa tgtggttcat tctatataca taacaattac tttcaacata
     3601 cataagccta cctgtatttg tgcattatat tacatggtac agataaatag tcatgtgccc
     3661 catgaacatt ttggtcaaag atagattaca gatacaatgg tggtcccata agattatgat
     3721 gccatatttt tactgtacct cttcttgtgc ctagatacac aaatactgac ttgtgttata
     3781 attgcctgca gtattcaata atatggtgta tagatttgta gcctacgagc tataacctat
     3841 actatatagc caaggagtgc aggagggtat gccatctaga tttgtgtgag tacactgatg
     3901 tttgaacaat aaaatcacct aatgataggt ttctcaaacc atatcccagt ccttaagcaa
     3961 cacatgacta atggattcaa ccatttcccc tgttcgttca gattgctttt tctttttgcc
     4021 cctacaattt tgttgataat actgtttttt ggggcttttg tttttgtttt tttttttttt
     4081 tttgagacaa gagtctcact ctgtcgtcag gctggagtgc aatggcgcta tctcagctca
     4141 ctgcaacctc tgcctcccag gttcaagcga ttctcctgcc tcagcctcct gagtagctga
     4201 gattacaggc atgcgccacc gcgcccggct aattttgtat ttttagtaga gacgcagttt
     4261 caccatgttg gtcaggctgg tcttgaacac ccgacctcag gtgatccacc cgcctcagct
     4321 tcccaaagtg ctgggattaa aggcgtgagc caccacacct ggccaattta ctgttttata
     4381 ccatgctttt ttgtgtgtat tctaggttcc ctaaagcaaa catgcatttt aaattttgat
     4441 agaaccagat gacatacaaa agtgcctgtt ttctctgcat tattaaaact gaaggtaatc
     4501 agtcttttat ttctatgact ccaactggca aaaaatattt gccattttaa cttttttttt
     4561 ttgagacgga gtcaaaaggt gccagttttc tctgcattat taaaactgaa ggtaatcagt
     4621 cttttatttc tatgactcca actggcaaaa gatatttgcc acgttaactt tttttttttt
     4681 ttttttgaga cggaggctca cactgtcacc caggctgaag tgcagtggca tgatctcagc
     4741 tcacttgcaa cctccgcctc ccaggttcaa gtgattctcc tgcctcagcc tcccaaggag
     4801 ttgggattac aggcgcatac ccggcttatt ttttgtattt ttagtagaaa cgggatttca
     4861 ccgtgttagc caggatggtc tcaatctcca gacttcgtga tcgcccacct cggcctccca
     4921 aagtgctggg attacaggcg tgagccaccg cgcccggccc tcagtaggcg atttttatat
     4981 gtggtataaa gtagaattct tacagatgga tacccgttta tgccagtgcc atgactgaaa
     5041 ccctgcttgt ccaacttgaa atatcttcaa tgcccatcta ttcttccttg atcttgtttt
     5101 ttcctgtgat aatacaatag tttttaccag ttaatcagtt aagatgaggc ttaccaaata
     5161 tatgggagta ctactgaaca cgatatcaag cttggcgtaa tcatggtcat agctgtttcc
     5221 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5281 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5341 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5401 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5461 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5521 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5581 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5641 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5701 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5761 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5821 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5881 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5941 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6001 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6061 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6121 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6361 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6421 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6481 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6541 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6601 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6661 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6721 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6781 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6841 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6901 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6961 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7021 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7081 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7141 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7201 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7261 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7321 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     7381 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
