LOCUS       Exported                7310 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SA_Kozak_npGal4-VP16_SynCoTC_UAS_mTagBFP2-T2A-splitGFP1-10_synCoTC 
            from 1 to 8084
            SA_Kozak_npGal4-VP16_SynCoTC_UAS_mTagBFP2-T2A-splitGFP11x7_synCoTC 
            from 1 to 8084
            Kozak_npGal4_F
            ctcagcgtaaagcttgccaccATGAAACTTCTATCTTCTATCGAACAGG
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     promoter        414..529
                     /locus_tag="4xnrUAS (Halpern)"
                     /label=4xnrUAS (Halpern)
     promoter        495..511
                     /locus_tag="UAS sequence"
                     /label=UAS sequence
     misc_feature    530..594
                     /locus_tag="E1B"
                     /label=E1B
     misc_feature    557..586
                     /locus_tag="carp beta-actin transcriptional start"
                     /label=carp beta-actin transcriptional start
     5'UTR           607..1295
                     /locus_tag="UBC intron (+1a)"
                     /label=UBC intron (+1a)
     misc_feature    1300..1308
                     /locus_tag="Kozak"
                     /label=Kozak
     misc_feature    join(1303,1306..1309)
                     /locus_tag="Vertebrate Kozak"
                     /label=Vertebrate Kozak
     misc_feature    1306..2010
                     /locus_tag="zf_mTagBFP2 (+2ndVal)"
                     /label=zf_mTagBFP2 (+2ndVal)
     misc_feature    1306..2007
                     /locus_tag="zf_mTagBFP2 (+2ndVal) noTAA"
                     /label=zf_mTagBFP2 (+2ndVal) noTAA
     polyA_signal    2022..2213
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     misc_feature    2229..2276
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    2277..2296
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    2307..2326
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    2330..2377
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     misc_feature    2524..2532
                     /locus_tag="Kozak(1)"
                     /label=Kozak
                     /label=Kozak(1)
     misc_feature    2530..3183
                     /locus_tag="zf.Gal4-VP16"
                     /label=zf.Gal4-VP16
     protein_bind    2530..2967
                     /locus_tag="zf.Gal4(DBD)"
                     /label=zf.Gal4(DBD)
     protein_bind    3007..3180
                     /locus_tag="zf.VP16 activation domain"
                     /label=zf.VP16 activation domain
     misc_feature    3190..5064
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    3190..3274
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      3755..5064
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(5085..5105)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(5102..5124)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(5111..5133)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(5473..6155)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(6250..7110)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(6253..6912)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggtggc
      421 ttctaatccg tgagtcctag cgggtgacag ccctccgtct tcacaggcgg aggagagtct
      481 tccgtagagt tcctcggagt actgtcctcc gacgcgtgca agggtcgact ctagagggta
      541 tataatggat cccatcgcgt ctcagcctca ctttgagctc ctccacacga attcgatact
      601 aaaagcttgt ttgtgagtat gatgttaaat ataagcaatc gacgtttgtt ttgtcgtttg
      661 ttaaatctag caatgtttta tttcaattaa agtgttaacc ggttcattta aaggtgtatt
      721 tctgaatgag ctaacgtatt agcttttaac gctagccgga ctgtttggtt ttgaggatca
      781 ataaaccaac gtttttttct gatttatttt gctttaaaat ataaaggtat aattcgtcgt
      841 cgagaatttt gggttttgtt cgagtttaga aatttggcgg cgtttttttg taccgcgaaa
      901 cagggcggtg gttcggtgag cttctcaatt catctgcttg acaaacggtt ttcggcaact
      961 gtaaatgcgc tatgatgctt ttattctttt cagcgtgtaa ctttgttaaa tataattgac
     1021 tttccgacta tcgacgttcg tttcatctcc gtgttattgt ttaatggcca ttttctgtgg
     1081 taaactgtcg ccattttgtt ttcagtcgtc cgtttgttcc gcccttactc acagctgtgt
     1141 cagcattagt gatgactgtt ctcacctggc ctgtttgctt tcattattga tgaagagctt
     1201 tctagagaat tctgaagctc gtctttgtcc tgaattcatc tgtattcatt taaaaaaaaa
     1261 aattaacctt aatttttttt tcttctcagc gtaaagcttg ccaccatggt gtccgaactt
     1321 atcaaagaaa atatgcatat gaagctgtac atggagggaa ctgtggacaa ccaccacttc
     1381 aagtgcacct ctgagggaga gggaaagcct tatgagggaa cccagaccat gcgcatcaag
     1441 gtggtggagg gaggacctct gccttttgct tttgacatcc tggccacctc tttcctgtat
     1501 ggaagcaaga ccttcatcaa ccacacccag ggaatccctg acttcttcaa gcagtctttc
     1561 cctgagggat tcacctggga aagagtgacc acctacgagg atggaggagt gctgaccgcc
     1621 acccaggaca cctctctcca ggatggatgc ctgatctaca acgtgaagat cagaggagtg
     1681 aactttacta gcaacggacc tgtgatgcaa aagaagaccc tgggatggga ggctttcact
     1741 gagaccctgt acccagctga tggaggactg gagggaagaa atgacatggc tctgaagctg
     1801 gtgggaggat ctcacctgat cgccaatgcc aagaccacct acagaagcaa gaagcctgcc
     1861 aagaacctga agatgcctgg agtgtactat gtggactaca gactggagcg catcaaggag
     1921 gccaacaatg agacctatgt ggagcagcat gaggtggctg tggccagata ttgtgacctc
     1981 cctagcaagc tgggacacaa gctgaattaa atacgtagat ccagacatga taagatacat
     2041 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2101 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2161 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttcgaatgc
     2221 atctagatga agttcctatt ccgaagttcc tattcttcaa atagtatagg aacttcgaag
     2281 atcggccact acattctggc ctaggaggct gctgcggttc cagaggtggg aagttcctat
     2341 tccgaagttc ctattctcta gaaagtatag gaacttcaat aaaagttaat ttaagcgttc
     2401 attacaataa attcaagtga atctgtttta agctagcctt taacgttttt aaattccttc
     2461 aaacaaattg gatcctcttt gcagttcctc atttctcggt gagctggtct cagcgtaaag
     2521 cttgccacca tgaaacttct atcttctatc gaacaggctt gcgacatctg ccgcctgaag
     2581 aagctgaagt gcagcaagga gaagccaaag tgcgccaagt gcctgaagaa caactgggag
     2641 tgcagatact ctcctaagac caagcgctct cctctgaccc gcgctcacct cactgaggtg
     2701 gagagcagac tggagagact ggagcagctg tttctcctga tcttcccaag agaggacctg
     2761 gacatgatcc tgaagatgga ctctctccag gacatcaagg ctctcctgac tggcctgttt
     2821 gtgcaggaca acgtgaacaa ggatgctgtg accgacagac tggcttctgt ggagaccgac
     2881 atgccactga ctctgagaca gcacagaatc tctgccacct cttcttctga ggagtctagc
     2941 aacaagggac agagacagct gactgtgtct agcagaagca cccctggaat ccagatcagc
     3001 agagctgctc ctccaactga tgtgtctctc ggagatgagc tccacctgga tggagaggat
     3061 gtggctatgg ctcatgctga tgctctggat gactttgacc tggacatgct cggagatgga
     3121 gactctccag gacctggatt cacccctcat gactctgctc cttatggcgc tctggacatg
     3181 taaactagta ttaagtctca gccaccgtta actgaacatg tcaaaacctg tggagactgt
     3241 tgagatttga tgttctgaaa agataaagcc tataaataaa acaatactca aatacaaaag
     3301 gtatcttttt taaaaagtta gtttactgct tccttaggct ctttctaaat cccacccacc
     3361 tgaaggacat caattatgga tttatccttc atgccttgta taaatacaga tgtgcatatg
     3421 aatttttatg ggttttttaa aaatgtggtt cattctatat acataacaat tactttcaac
     3481 atacataagc ctacctgtat ttgtgcatta tattacatgg tacagataaa tagtcatgtg
     3541 ccccatgaac attttggtca aagatagatt acagatacaa tggtggtccc ataagattat
     3601 gatgccatat ttttactgta cctcttcttg tgcctagata cacaaatact gacttgtgtt
     3661 ataattgcct gcagtattca ataatatggt gtatagattt gtagcctacg agctataacc
     3721 tatactatat agccaaggag tgcaggaggg tatgccatct agatttgtgt gagtacactg
     3781 atgtttgaac aataaaatca cctaatgata ggtttctcaa accatatccc agtccttaag
     3841 caacacatga ctaatggatt caaccatttc ccctgttcgt tcagattgct ttttcttttt
     3901 gcccctacaa ttttgttgat aatactgttt tttggggctt ttgtttttgt tttttttttt
     3961 ttttttgaga caagagtctc actctgtcgt caggctggag tgcaatggcg ctatctcagc
     4021 tcactgcaac ctctgcctcc caggttcaag cgattctcct gcctcagcct cctgagtagc
     4081 tgagattaca ggcatgcgcc accgcgcccg gctaattttg tatttttagt agagacgcag
     4141 tttcaccatg ttggtcaggc tggtcttgaa cacccgacct caggtgatcc acccgcctca
     4201 gcttcccaaa gtgctgggat taaaggcgtg agccaccaca cctggccaat ttactgtttt
     4261 ataccatgct tttttgtgtg tattctaggt tccctaaagc aaacatgcat tttaaatttt
     4321 gatagaacca gatgacatac aaaagtgcct gttttctctg cattattaaa actgaaggta
     4381 atcagtcttt tatttctatg actccaactg gcaaaaaata tttgccattt taactttttt
     4441 tttttgagac ggagtcaaaa ggtgccagtt ttctctgcat tattaaaact gaaggtaatc
     4501 agtcttttat ttctatgact ccaactggca aaagatattt gccacgttaa cttttttttt
     4561 tttttttttg agacggaggc tcacactgtc acccaggctg aagtgcagtg gcatgatctc
     4621 agctcacttg caacctccgc ctcccaggtt caagtgattc tcctgcctca gcctcccaag
     4681 gagttgggat tacaggcgca tacccggctt attttttgta tttttagtag aaacgggatt
     4741 tcaccgtgtt agccaggatg gtctcaatct ccagacttcg tgatcgccca cctcggcctc
     4801 ccaaagtgct gggattacag gcgtgagcca ccgcgcccgg ccctcagtag gcgattttta
     4861 tatgtggtat aaagtagaat tcttacagat ggatacccgt ttatgccagt gccatgactg
     4921 aaaccctgct tgtccaactt gaaatatctt caatgcccat ctattcttcc ttgatcttgt
     4981 tttttcctgt gataatacaa tagtttttac cagttaatca gttaagatga ggcttaccaa
     5041 atatatggga gtactactga acacgatatc aagcttggcg taatcatggt catagctgtt
     5101 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5161 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5221 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5281 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5341 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5401 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5461 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5521 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5581 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5641 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5701 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5761 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5821 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5881 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5941 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6001 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6061 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6121 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6181 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6241 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6301 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6361 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6421 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6481 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6541 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6601 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6661 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6721 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6781 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6841 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6901 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6961 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7021 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7081 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7141 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7201 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     7261 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
