LOCUS       Exported                8084 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     SA_Kozak_npGal4-VP16_SynCoTC_UAS_mTagBFP2-T2A-splitGFP11x7_synCoTC 
            from 1 to 8084
            Kozak_npGal4_F
            ctcagcgtaaagcttgccaccATGAAACTTCTATCTTCTATCGAACAGG
FEATURES             Location/Qualifiers
     source          1..8084
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     promoter        414..529
                     /locus_tag="4xnrUAS (Halpern)"
                     /label=4xnrUAS (Halpern)
     promoter        495..511
                     /locus_tag="UAS sequence"
                     /label=UAS sequence
     misc_feature    530..594
                     /locus_tag="E1B"
                     /label=E1B
     misc_feature    557..586
                     /locus_tag="carp beta-actin transcriptional start"
                     /label=carp beta-actin transcriptional start
     5'UTR           607..1295
                     /locus_tag="UBC intron (+1a)"
                     /label=UBC intron (+1a)
     misc_feature    1300..1308
                     /locus_tag="Kozak"
                     /label=Kozak
     misc_feature    join(1303,1306..1309)
                     /locus_tag="Vertebrate Kozak"
                     /label=Vertebrate Kozak
     misc_feature    1306..2007
                     /locus_tag="zf_mTagBFP2 (+2ndVal) noTAA"
                     /label=zf_mTagBFP2 (+2ndVal) noTAA
     variation       2008..2022
                     /locus_tag="(GGGGS)linker"
                     /label=(GGGGS)linker
     misc_feature    2023..2064
                     /locus_tag="V5-tag zf-optim (GKPIPNPLLGLDST)"
                     /label=V5-tag zf-optim (GKPIPNPLLGLDST)
     protein_bind    2065..2118
                     /locus_tag="zf_opt-T2A"
                     /label=zf_opt-T2A
     misc_binding    2119..2763
                     /locus_tag="zf-sGFP1-10"
                     /label=zf-sGFP1-10
     polyA_signal    2796..2987
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     misc_feature    3003..3050
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    3051..3070
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    3081..3100
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    3104..3151
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     misc_feature    3298..3306
                     /locus_tag="Kozak(1)"
                     /label=Kozak
                     /label=Kozak(1)
     misc_feature    3304..3957
                     /locus_tag="zf.Gal4-VP16"
                     /label=zf.Gal4-VP16
     protein_bind    3304..3741
                     /locus_tag="zf.Gal4(DBD)"
                     /label=zf.Gal4(DBD)
     protein_bind    3781..3954
                     /locus_tag="zf.VP16 activation domain"
                     /label=zf.VP16 activation domain
     misc_feature    3964..5838
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    3964..4048
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      4529..5838
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(5859..5879)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(5876..5898)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(5885..5907)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(6247..6929)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(7024..7884)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(7027..7686)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggtggc
      421 ttctaatccg tgagtcctag cgggtgacag ccctccgtct tcacaggcgg aggagagtct
      481 tccgtagagt tcctcggagt actgtcctcc gacgcgtgca agggtcgact ctagagggta
      541 tataatggat cccatcgcgt ctcagcctca ctttgagctc ctccacacga attcgatact
      601 aaaagcttgt ttgtgagtat gatgttaaat ataagcaatc gacgtttgtt ttgtcgtttg
      661 ttaaatctag caatgtttta tttcaattaa agtgttaacc ggttcattta aaggtgtatt
      721 tctgaatgag ctaacgtatt agcttttaac gctagccgga ctgtttggtt ttgaggatca
      781 ataaaccaac gtttttttct gatttatttt gctttaaaat ataaaggtat aattcgtcgt
      841 cgagaatttt gggttttgtt cgagtttaga aatttggcgg cgtttttttg taccgcgaaa
      901 cagggcggtg gttcggtgag cttctcaatt catctgcttg acaaacggtt ttcggcaact
      961 gtaaatgcgc tatgatgctt ttattctttt cagcgtgtaa ctttgttaaa tataattgac
     1021 tttccgacta tcgacgttcg tttcatctcc gtgttattgt ttaatggcca ttttctgtgg
     1081 taaactgtcg ccattttgtt ttcagtcgtc cgtttgttcc gcccttactc acagctgtgt
     1141 cagcattagt gatgactgtt ctcacctggc ctgtttgctt tcattattga tgaagagctt
     1201 tctagagaat tctgaagctc gtctttgtcc tgaattcatc tgtattcatt taaaaaaaaa
     1261 aattaacctt aatttttttt tcttctcagc gtaaagcttg ccaccatggt gtccgaactt
     1321 atcaaagaaa atatgcatat gaagctgtac atggagggaa ctgtggacaa ccaccacttc
     1381 aagtgcacct ctgagggaga gggaaagcct tatgagggaa cccagaccat gcgcatcaag
     1441 gtggtggagg gaggacctct gccttttgct tttgacatcc tggccacctc tttcctgtat
     1501 ggaagcaaga ccttcatcaa ccacacccag ggaatccctg acttcttcaa gcagtctttc
     1561 cctgagggat tcacctggga aagagtgacc acctacgagg atggaggagt gctgaccgcc
     1621 acccaggaca cctctctcca ggatggatgc ctgatctaca acgtgaagat cagaggagtg
     1681 aactttacta gcaacggacc tgtgatgcaa aagaagaccc tgggatggga ggctttcact
     1741 gagaccctgt acccagctga tggaggactg gagggaagaa atgacatggc tctgaagctg
     1801 gtgggaggat ctcacctgat cgccaatgcc aagaccacct acagaagcaa gaagcctgcc
     1861 aagaacctga agatgcctgg agtgtactat gtggactaca gactggagcg catcaaggag
     1921 gccaacaatg agacctatgt ggagcagcat gaggtggctg tggccagata ttgtgacctc
     1981 cctagcaagc tgggacacaa gctgaatgga ggaggaggat ctggaaagcc aatcccaaac
     2041 cctctgctgg gactggacag caccgaggga agaggatctc tcctgacctg tggagatgtg
     2101 gaggagaacc ctggcccaat gagcaaggga gaggagctgt ttactggagt ggtgccaatc
     2161 ctggtggagc tggatggaga cgtgaatgga cacaaattct ctgtgagagg agagggagag
     2221 ggagatgcca ccattggaaa gctgaccctg aagttcattt gtaccaccgg aaagctgcct
     2281 gtcccttggc caaccctggt gaccaccctg acctatggag tgcagtgctt cagcagatac
     2341 cctgaccaca tgaagagaca tgacttcttc aagtctgcca tgcctgaggg atatgtgcag
     2401 gagagaacca tctctttcaa ggatgatgga aagtacaaga ccagagctgt ggtgaagttt
     2461 gagggagaca ccctggtgaa cagaattgag ctgaaaggca ctgacttcaa ggaggatgga
     2521 aacatcctgg gacacaagct ggagtacaac ttcaactctc acaatgtgta catcaccgct
     2581 gataagcaga agaatggcat caaggccaac ttcaccgtga gacacaatgt ggaggatgga
     2641 tctgtgcagc tggctgatca ctatcagcag aacaccccaa ttggagacgg acctgtgctg
     2701 ctgcctgata atcattacct gagcacccag actgtgctga gcaaggaccc aaatgagaag
     2761 taaactagta ttaagtctca gccaccgtgt agatccagac atgataagat acattgatga
     2821 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2881 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2941 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttcga atgcatctag
     3001 atgaagttcc tattccgaag ttcctattct tcaaatagta taggaacttc gaagatcggc
     3061 cactacattc tggcctagga ggctgctgcg gttccagagg tgggaagttc ctattccgaa
     3121 gttcctattc tctagaaagt ataggaactt caataaaagt taatttaagc gttcattaca
     3181 ataaattcaa gtgaatctgt tttaagctag cctttaacgt ttttaaattc cttcaaacaa
     3241 attggatcct ctttgcagtt cctcatttct cggtgagctg gtctcagcgt aaagcttgcc
     3301 accatgaaac ttctatcttc tatcgaacag gcttgcgaca tctgccgcct gaagaagctg
     3361 aagtgcagca aggagaagcc aaagtgcgcc aagtgcctga agaacaactg ggagtgcaga
     3421 tactctccta agaccaagcg ctctcctctg acccgcgctc acctcactga ggtggagagc
     3481 agactggaga gactggagca gctgtttctc ctgatcttcc caagagagga cctggacatg
     3541 atcctgaaga tggactctct ccaggacatc aaggctctcc tgactggcct gtttgtgcag
     3601 gacaacgtga acaaggatgc tgtgaccgac agactggctt ctgtggagac cgacatgcca
     3661 ctgactctga gacagcacag aatctctgcc acctcttctt ctgaggagtc tagcaacaag
     3721 ggacagagac agctgactgt gtctagcaga agcacccctg gaatccagat cagcagagct
     3781 gctcctccaa ctgatgtgtc tctcggagat gagctccacc tggatggaga ggatgtggct
     3841 atggctcatg ctgatgctct ggatgacttt gacctggaca tgctcggaga tggagactct
     3901 ccaggacctg gattcacccc tcatgactct gctccttatg gcgctctgga catgtaaact
     3961 agtattaagt ctcagccacc gttaactgaa catgtcaaaa cctgtggaga ctgttgagat
     4021 ttgatgttct gaaaagataa agcctataaa taaaacaata ctcaaataca aaaggtatct
     4081 tttttaaaaa gttagtttac tgcttcctta ggctctttct aaatcccacc cacctgaagg
     4141 acatcaatta tggatttatc cttcatgcct tgtataaata cagatgtgca tatgaatttt
     4201 tatgggtttt ttaaaaatgt ggttcattct atatacataa caattacttt caacatacat
     4261 aagcctacct gtatttgtgc attatattac atggtacaga taaatagtca tgtgccccat
     4321 gaacattttg gtcaaagata gattacagat acaatggtgg tcccataaga ttatgatgcc
     4381 atatttttac tgtacctctt cttgtgccta gatacacaaa tactgacttg tgttataatt
     4441 gcctgcagta ttcaataata tggtgtatag atttgtagcc tacgagctat aacctatact
     4501 atatagccaa ggagtgcagg agggtatgcc atctagattt gtgtgagtac actgatgttt
     4561 gaacaataaa atcacctaat gataggtttc tcaaaccata tcccagtcct taagcaacac
     4621 atgactaatg gattcaacca tttcccctgt tcgttcagat tgctttttct ttttgcccct
     4681 acaattttgt tgataatact gttttttggg gcttttgttt ttgttttttt tttttttttt
     4741 gagacaagag tctcactctg tcgtcaggct ggagtgcaat ggcgctatct cagctcactg
     4801 caacctctgc ctcccaggtt caagcgattc tcctgcctca gcctcctgag tagctgagat
     4861 tacaggcatg cgccaccgcg cccggctaat tttgtatttt tagtagagac gcagtttcac
     4921 catgttggtc aggctggtct tgaacacccg acctcaggtg atccacccgc ctcagcttcc
     4981 caaagtgctg ggattaaagg cgtgagccac cacacctggc caatttactg ttttatacca
     5041 tgcttttttg tgtgtattct aggttcccta aagcaaacat gcattttaaa ttttgataga
     5101 accagatgac atacaaaagt gcctgttttc tctgcattat taaaactgaa ggtaatcagt
     5161 cttttatttc tatgactcca actggcaaaa aatatttgcc attttaactt tttttttttg
     5221 agacggagtc aaaaggtgcc agttttctct gcattattaa aactgaaggt aatcagtctt
     5281 ttatttctat gactccaact ggcaaaagat atttgccacg ttaacttttt tttttttttt
     5341 tttgagacgg aggctcacac tgtcacccag gctgaagtgc agtggcatga tctcagctca
     5401 cttgcaacct ccgcctccca ggttcaagtg attctcctgc ctcagcctcc caaggagttg
     5461 ggattacagg cgcatacccg gcttattttt tgtattttta gtagaaacgg gatttcaccg
     5521 tgttagccag gatggtctca atctccagac ttcgtgatcg cccacctcgg cctcccaaag
     5581 tgctgggatt acaggcgtga gccaccgcgc ccggccctca gtaggcgatt tttatatgtg
     5641 gtataaagta gaattcttac agatggatac ccgtttatgc cagtgccatg actgaaaccc
     5701 tgcttgtcca acttgaaata tcttcaatgc ccatctattc ttccttgatc ttgttttttc
     5761 ctgtgataat acaatagttt ttaccagtta atcagttaag atgaggctta ccaaatatat
     5821 gggagtacta ctgaacacga tatcaagctt ggcgtaatca tggtcatagc tgtttcctgt
     5881 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     5941 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6001 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6061 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6121 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6181 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6241 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6841 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6901 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6961 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7021 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7081 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7141 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7201 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7261 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7321 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7381 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7441 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7501 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7561 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7621 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7681 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7741 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7801 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7861 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7921 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7981 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     8041 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
