LOCUS       Exported                7868 bp ds-DNA     circular SYN 06-MAR-2023
DEFINITION  pUC57.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Kozak_npGal4_F
            ctcagcgtaaagcttgccaccATGAAACTTCTATCTTCTATCGAACAGG
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(239..307)
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     primer_bind     364..386
                     /locus_tag="m13/pUC Forward"
                     /label=m13/pUC Forward
     primer_bind     378..395
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     promoter        414..529
                     /locus_tag="4xnrUAS (Halpern)"
                     /label=4xnrUAS (Halpern)
     promoter        495..511
                     /locus_tag="UAS sequence"
                     /label=UAS sequence
     misc_feature    530..594
                     /locus_tag="E1B"
                     /label=E1B
     misc_feature    557..586
                     /locus_tag="carp beta-actin transcriptional start"
                     /label=carp beta-actin transcriptional start
     5'UTR           607..1295
                     /locus_tag="UBC intron (+1a)"
                     /label=UBC intron (+1a)
     misc_feature    1300..1308
                     /locus_tag="Kozak"
                     /label=Kozak
     misc_feature    join(1303,1306..1309)
                     /locus_tag="Vertebrate Kozak"
                     /label=Vertebrate Kozak
     misc_feature    1306..2007
                     /locus_tag="zf_mTagBFP2 (+2ndVal) noTAA"
                     /label=zf_mTagBFP2 (+2ndVal) noTAA
     variation       2008..2022
                     /locus_tag="(GGGGS)linker"
                     /label=(GGGGS)linker
     misc_feature    2023..2064
                     /locus_tag="V5-tag zf-optim (GKPIPNPLLGLDST)"
                     /label=V5-tag zf-optim (GKPIPNPLLGLDST)
     protein_bind    2065..2118
                     /locus_tag="zf_opt-T2A"
                     /label=zf_opt-T2A
     misc_feature    2119..2547
                     /locus_tag="zf-sGFP11x7 (5aa linker)"
                     /label=zf-sGFP11x7 (5aa linker)
     misc_binding    2119..2166
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
     misc_binding    2182..2229
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(1)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(1)
     misc_binding    2245..2292
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(2)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(2)
     misc_binding    2308..2355
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(3)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(3)
     misc_binding    2371..2418
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(4)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(4)
     misc_binding    2434..2481
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(5)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(5)
     misc_binding    2497..2544
                     /locus_tag="zf-GFP11(RDHMVLHEYVNAAGIT)(6)"
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)
                     /label=zf-GFP11(RDHMVLHEYVNAAGIT)(6)
     polyA_signal    2580..2771
                     /locus_tag="SV40 late polyA"
                     /label=SV40 late polyA
     misc_feature    2787..2834
                     /locus_tag="48bp_FRT3"
                     /label=48bp_FRT3
     misc_feature    2835..2854
                     /locus_tag="sgH (human ampka1 guide)"
                     /label=sgH (human ampka1 guide)
     misc_feature    2865..2884
                     /locus_tag="sgM site"
                     /label=sgM site
     misc_feature    2888..2935
                     /locus_tag="48bp_FRT"
                     /label=48bp_FRT
     misc_feature    3082..3090
                     /locus_tag="Kozak(1)"
                     /label=Kozak
                     /label=Kozak(1)
     misc_feature    3088..3741
                     /locus_tag="zf.Gal4-VP16"
                     /label=zf.Gal4-VP16
     protein_bind    3088..3525
                     /locus_tag="zf.Gal4(DBD)"
                     /label=zf.Gal4(DBD)
     protein_bind    3565..3738
                     /locus_tag="zf.VP16 activation domain"
                     /label=zf.VP16 activation domain
     misc_feature    3748..5622
                     /locus_tag="synCoTC"
                     /label=synCoTC
     misc_feature    3748..3832
                     /locus_tag="afp 3'UTR (truncated at polyA)"
                     /label=afp 3'UTR (truncated at polyA)
     terminator      4313..5622
                     /locus_tag="CoTC terminator element"
                     /label=CoTC terminator element
     primer_bind     complement(5643..5663)
                     /locus_tag="M13-rev"
                     /label=M13-rev
     primer_bind     complement(5660..5682)
                     /locus_tag="m13/pUC Reverse"
                     /label=m13/pUC Reverse
     misc_binding    complement(5669..5691)
                     /locus_tag="LacO"
                     /label=LacO
     rep_origin      complement(6031..6713)
                     /direction=LEFT
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    complement(6808..7668)
                     /locus_tag="AmpR gene"
                     /label=AmpR gene
     CDS             complement(6811..7470)
                     /locus_tag="AmpR"
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggtggc
      421 ttctaatccg tgagtcctag cgggtgacag ccctccgtct tcacaggcgg aggagagtct
      481 tccgtagagt tcctcggagt actgtcctcc gacgcgtgca agggtcgact ctagagggta
      541 tataatggat cccatcgcgt ctcagcctca ctttgagctc ctccacacga attcgatact
      601 aaaagcttgt ttgtgagtat gatgttaaat ataagcaatc gacgtttgtt ttgtcgtttg
      661 ttaaatctag caatgtttta tttcaattaa agtgttaacc ggttcattta aaggtgtatt
      721 tctgaatgag ctaacgtatt agcttttaac gctagccgga ctgtttggtt ttgaggatca
      781 ataaaccaac gtttttttct gatttatttt gctttaaaat ataaaggtat aattcgtcgt
      841 cgagaatttt gggttttgtt cgagtttaga aatttggcgg cgtttttttg taccgcgaaa
      901 cagggcggtg gttcggtgag cttctcaatt catctgcttg acaaacggtt ttcggcaact
      961 gtaaatgcgc tatgatgctt ttattctttt cagcgtgtaa ctttgttaaa tataattgac
     1021 tttccgacta tcgacgttcg tttcatctcc gtgttattgt ttaatggcca ttttctgtgg
     1081 taaactgtcg ccattttgtt ttcagtcgtc cgtttgttcc gcccttactc acagctgtgt
     1141 cagcattagt gatgactgtt ctcacctggc ctgtttgctt tcattattga tgaagagctt
     1201 tctagagaat tctgaagctc gtctttgtcc tgaattcatc tgtattcatt taaaaaaaaa
     1261 aattaacctt aatttttttt tcttctcagc gtaaagcttg ccaccatggt gtccgaactt
     1321 atcaaagaaa atatgcatat gaagctgtac atggagggaa ctgtggacaa ccaccacttc
     1381 aagtgcacct ctgagggaga gggaaagcct tatgagggaa cccagaccat gcgcatcaag
     1441 gtggtggagg gaggacctct gccttttgct tttgacatcc tggccacctc tttcctgtat
     1501 ggaagcaaga ccttcatcaa ccacacccag ggaatccctg acttcttcaa gcagtctttc
     1561 cctgagggat tcacctggga aagagtgacc acctacgagg atggaggagt gctgaccgcc
     1621 acccaggaca cctctctcca ggatggatgc ctgatctaca acgtgaagat cagaggagtg
     1681 aactttacta gcaacggacc tgtgatgcaa aagaagaccc tgggatggga ggctttcact
     1741 gagaccctgt acccagctga tggaggactg gagggaagaa atgacatggc tctgaagctg
     1801 gtgggaggat ctcacctgat cgccaatgcc aagaccacct acagaagcaa gaagcctgcc
     1861 aagaacctga agatgcctgg agtgtactat gtggactaca gactggagcg catcaaggag
     1921 gccaacaatg agacctatgt ggagcagcat gaggtggctg tggccagata ttgtgacctc
     1981 cctagcaagc tgggacacaa gctgaatgga ggaggaggat ctggaaagcc aatcccaaac
     2041 cctctgctgg gactggacag caccgaggga agaggatctc tcctgacctg tggagatgtg
     2101 gaggagaacc ctggcccaag agaccacatg gtcctgcatg agtacgtgaa cgccgctgga
     2161 attactggag gatctggagg aagagaccac atggtcctgc atgagtacgt gaacgccgct
     2221 ggaattactg gaggatctgg aggaagagac cacatggtcc tgcatgagta cgtgaacgcc
     2281 gctggaatta ctggaggatc tggaggaaga gaccacatgg tcctgcatga gtacgtgaac
     2341 gccgctggaa ttactggagg atctggagga agagaccaca tggtcctgca tgagtacgtg
     2401 aacgccgctg gaattactgg aggatctgga ggaagagacc acatggtcct gcatgagtac
     2461 gtgaacgccg ctggaattac tggaggatct ggaggaagag accacatggt cctgcatgag
     2521 tacgtgaacg ccgctggaat tacttaaact agtattaagt ctcagccacc gtgtagatcc
     2581 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2641 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2701 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     2761 ggaggttttt tcgaatgcat ctagatgaag ttcctattcc gaagttccta ttcttcaaat
     2821 agtataggaa cttcgaagat cggccactac attctggcct aggaggctgc tgcggttcca
     2881 gaggtgggaa gttcctattc cgaagttcct attctctaga aagtatagga acttcaataa
     2941 aagttaattt aagcgttcat tacaataaat tcaagtgaat ctgttttaag ctagccttta
     3001 acgtttttaa attccttcaa acaaattgga tcctctttgc agttcctcat ttctcggtga
     3061 gctggtctca gcgtaaagct tgccaccatg aaacttctat cttctatcga acaggcttgc
     3121 gacatctgcc gcctgaagaa gctgaagtgc agcaaggaga agccaaagtg cgccaagtgc
     3181 ctgaagaaca actgggagtg cagatactct cctaagacca agcgctctcc tctgacccgc
     3241 gctcacctca ctgaggtgga gagcagactg gagagactgg agcagctgtt tctcctgatc
     3301 ttcccaagag aggacctgga catgatcctg aagatggact ctctccagga catcaaggct
     3361 ctcctgactg gcctgtttgt gcaggacaac gtgaacaagg atgctgtgac cgacagactg
     3421 gcttctgtgg agaccgacat gccactgact ctgagacagc acagaatctc tgccacctct
     3481 tcttctgagg agtctagcaa caagggacag agacagctga ctgtgtctag cagaagcacc
     3541 cctggaatcc agatcagcag agctgctcct ccaactgatg tgtctctcgg agatgagctc
     3601 cacctggatg gagaggatgt ggctatggct catgctgatg ctctggatga ctttgacctg
     3661 gacatgctcg gagatggaga ctctccagga cctggattca cccctcatga ctctgctcct
     3721 tatggcgctc tggacatgta aactagtatt aagtctcagc caccgttaac tgaacatgtc
     3781 aaaacctgtg gagactgttg agatttgatg ttctgaaaag ataaagccta taaataaaac
     3841 aatactcaaa tacaaaaggt atctttttta aaaagttagt ttactgcttc cttaggctct
     3901 ttctaaatcc cacccacctg aaggacatca attatggatt tatccttcat gccttgtata
     3961 aatacagatg tgcatatgaa tttttatggg ttttttaaaa atgtggttca ttctatatac
     4021 ataacaatta ctttcaacat acataagcct acctgtattt gtgcattata ttacatggta
     4081 cagataaata gtcatgtgcc ccatgaacat tttggtcaaa gatagattac agatacaatg
     4141 gtggtcccat aagattatga tgccatattt ttactgtacc tcttcttgtg cctagataca
     4201 caaatactga cttgtgttat aattgcctgc agtattcaat aatatggtgt atagatttgt
     4261 agcctacgag ctataaccta tactatatag ccaaggagtg caggagggta tgccatctag
     4321 atttgtgtga gtacactgat gtttgaacaa taaaatcacc taatgatagg tttctcaaac
     4381 catatcccag tccttaagca acacatgact aatggattca accatttccc ctgttcgttc
     4441 agattgcttt ttctttttgc ccctacaatt ttgttgataa tactgttttt tggggctttt
     4501 gtttttgttt tttttttttt ttttgagaca agagtctcac tctgtcgtca ggctggagtg
     4561 caatggcgct atctcagctc actgcaacct ctgcctccca ggttcaagcg attctcctgc
     4621 ctcagcctcc tgagtagctg agattacagg catgcgccac cgcgcccggc taattttgta
     4681 tttttagtag agacgcagtt tcaccatgtt ggtcaggctg gtcttgaaca cccgacctca
     4741 ggtgatccac ccgcctcagc ttcccaaagt gctgggatta aaggcgtgag ccaccacacc
     4801 tggccaattt actgttttat accatgcttt tttgtgtgta ttctaggttc cctaaagcaa
     4861 acatgcattt taaattttga tagaaccaga tgacatacaa aagtgcctgt tttctctgca
     4921 ttattaaaac tgaaggtaat cagtctttta tttctatgac tccaactggc aaaaaatatt
     4981 tgccatttta actttttttt tttgagacgg agtcaaaagg tgccagtttt ctctgcatta
     5041 ttaaaactga aggtaatcag tcttttattt ctatgactcc aactggcaaa agatatttgc
     5101 cacgttaact tttttttttt tttttttgag acggaggctc acactgtcac ccaggctgaa
     5161 gtgcagtggc atgatctcag ctcacttgca acctccgcct cccaggttca agtgattctc
     5221 ctgcctcagc ctcccaagga gttgggatta caggcgcata cccggcttat tttttgtatt
     5281 tttagtagaa acgggatttc accgtgttag ccaggatggt ctcaatctcc agacttcgtg
     5341 atcgcccacc tcggcctccc aaagtgctgg gattacaggc gtgagccacc gcgcccggcc
     5401 ctcagtaggc gatttttata tgtggtataa agtagaattc ttacagatgg atacccgttt
     5461 atgccagtgc catgactgaa accctgcttg tccaacttga aatatcttca atgcccatct
     5521 attcttcctt gatcttgttt tttcctgtga taatacaata gtttttacca gttaatcagt
     5581 taagatgagg cttaccaaat atatgggagt actactgaac acgatatcaa gcttggcgta
     5641 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5701 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5761 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5821 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5881 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5941 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6001 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6061 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6121 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6181 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6241 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6301 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6361 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6421 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6481 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6541 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6601 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6661 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6721 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6781 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6841 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6901 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6961 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7021 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7081 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7141 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7201 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7261 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7321 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7381 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7441 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7501 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7561 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7621 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7681 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7741 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7801 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     7861 ctttcgtc
//
