LOCUS       Exported                9174 bp ds-DNA     circular SYN 15-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 16, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..413
                     /locus_tag="5' LTR"
                     /label=5' LTR
     misc_feature    464..601
                     /locus_tag="psi"
                     /label=psi
     misc_feature    568..932
                     /locus_tag="gag"
                     /label=gag
     misc_feature    1078..1319
                     /locus_tag="RRE"
                     /label=RRE
     misc_feature    1752..1869
                     /locus_tag="cPPT"
                     /label=cPPT
     promoter        1922..2297
                     /locus_tag="TRE3G"
                     /label=TRE3G
     misc_feature    2302..2307
                     /locus_tag="NheI"
                     /label=NheI
     misc_feature    2308..2313
                     /locus_tag="Kozak sequence"
                     /label=Kozak sequence
     CDS             2314..2316
                     /codon_start=1
                     /locus_tag="Start"
                     /label=Start
                     /translation="M"
     misc_feature    2317..2358
                     /locus_tag="V5 tag"
                     /label=V5 tag
     misc_feature    2359..2364
                     /locus_tag="KpnI"
                     /label=KpnI
     misc_feature    2365..3726
                     /locus_tag="LOV-Turbo, human codon optimized"
                     /label=LOV-Turbo, human codon optimized
     misc_feature    2515..2517
                     /locus_tag="T52S"
                     /label=T52S
     misc_feature    2578..2580
                     /locus_tag="L73Q"
                     /label=L73Q
     misc_feature    2590..3006
                     /locus_tag="LOV"
                     /label=LOV
     misc_feature    3202..3204
                     /locus_tag="I147T"
                     /label=I147T
     misc_feature    3259..3261
                     /locus_tag="A166V"
                     /label=A166V
     misc_feature    3454..3456
                     /locus_tag="I231V"
                     /label=I231V
     misc_feature    3619..3621
                     /locus_tag="F286L"
                     /label=F286L
     misc_feature    3727..3732
                     /locus_tag="BamHI"
                     /label=BamHI
     misc_feature    3733..3768
                     /locus_tag="NES"
                     /label=NES
     misc_feature    3769..3774
                     /locus_tag="Stop"
                     /label=Stop
     misc_feature    3779..3784
                     /locus_tag="EcoRI"
                     /label=EcoRI
     misc_feature    3785..4373
                     /locus_tag="WPRE"
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(3838..3858)
                     /locus_tag="WPRE-R"
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4256..4267)
                     /codon_start=1
                     /locus_tag="Factor Xa site"
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4393..4903
                     /locus_tag="hPGK promoter"
                     /label=hPGK promoter
     CDS             4913..5656
                     /locus_tag="Tet-On 3G"
                     /label=Tet-On 3G
     misc_feature    5657..5722
                     /locus_tag="P2A"
                     /label=P2A
     misc_feature    5723..6316
                     /locus_tag="puro"
                     /label=puro
     misc_feature    5939..5945
                     /locus_tag="RR47"
                     /label=RR47
     misc_feature    5940..5945
                     /locus_tag="RR48"
                     /label=RR48
     misc_feature    6417..6652
                     /locus_tag="3' LTR"
                     /label=3' LTR
     misc_feature    6723..6853
                     /locus_tag="PolyA signal"
                     /label=PolyA signal
     misc_feature    7091..7951
                     /locus_tag="AmpR"
                     /label=AmpR
     misc_feature    8212..8827
                     /locus_tag="pUC/Origin"
                     /label=pUC/Origin
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga caattctcga cctcgagaca aatggcagta
     1741 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1801 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1861 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg aatcgatatg
     1921 ttttactccc tatcagtgat agagaacgta tgaagagttt actccctatc agtgatagag
     1981 aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt ttactcccta
     2041 tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa cgtatctaca
     2101 gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc agtgatagag
     2161 aacgtataag ctttaggcgt gtacggtggg cgcctataaa agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagca attccacaac acttttgtct tataccaact ttccgtacca
     2281 cttcctaccc tcgtaaatag agctagcgcc accatgggca agcccatccc caaccccctg
     2341 ctgggcctgg acagcaccgg taccaaagac aacacagtgc ctctgaagct gatcgccctc
     2401 ctggccaatg gagagttcca cagcggcgag cagctgggcg aaaccctggg catgagcaga
     2461 gccgctatca acaagcacat ccagaccctt cgcgactggg gcgtcgacgt gttcagcgtg
     2521 cccggcaagg gctacagcct gcctgagcct atccccctgc tgaacgccaa gcagattcag
     2581 ggccagctgg aatttagagc caccaccctg gaaagaatcg agaagtcttt tgtgatcacc
     2641 gaccccagac tgcctgataa tcctatcatc ttcgtgtctg atagcttcct gcagctcacc
     2701 gagtattctc gggaagagat cctcggaaga aattgcagat tcctgcaggg cccagagaca
     2761 gatagagcca cagtgcggaa gatcagagat gccatcgaca accagacaga ggtgaccgtg
     2821 cagctgatca actacaccaa gtccggcaaa aagttctgga acgttttcca tctccaacct
     2881 atgagagact acaagggcga cgtgcagtac ttcatcggag ttcagctgga cggcaccgag
     2941 agactgcacg gcgccgcaga gcgggaagct gtgtgcctgg tcaagaagac cgccttccag
     3001 atcgccgtgg ctgtgctgcc cgtggtggac agcacaaacc agtacctgct ggacagaatc
     3061 ggcgagctga agtccggcga cgcttgtatc gccgaatacc agcaggccgg cagaggcagc
     3121 agaggccgga aatggttcag ccccttcggc gccaacctgt acctgagcat gttttggcgg
     3181 ctgaaaagag gccctgccgc cacaggcctg ggccccgtga taggaatcgt gatggccgag
     3241 gctctgagga agctgggtgt ggacaaagtg cgggtgaagt ggcctaacga cctgtacctg
     3301 caggacagaa agctggccgg catcctggtg gaactggctg gcatcaccgg cgacgccgct
     3361 cagattgtga tcggcgctgg catcaatgtg gccatgagac gcgtggaaga gagcgtggtc
     3421 aaccagggct ggatcacact gcaagaggcc ggtgtgaacc tggataggaa caccctggcc
     3481 gccaccctga tcagagagct gagagccgct ctggaattgt ttgagcagga gggcctggcc
     3541 ccttacctgc cacggtggga gaaactggat aacttcatca acaggcctgt gaagctgatc
     3601 atcggcgata aggaaatcct gggaatcagc cggggcatcg acaagcaggg agccctgctg
     3661 ctggaacaag atggagtgat taagccttgg atgggcggag aaatcagcct gcggtccgcc
     3721 gagaagggat ccctgcagct gcctcccctg gagcgcctga ccctggacta atagggccga
     3781 attcaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3961 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4021 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4081 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4141 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4201 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4261 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4321 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgtctg
     4381 cagatatcat ccggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
     4441 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     4501 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     4561 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     4621 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     4681 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     4741 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     4801 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     4861 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagcaattca ccatgtctag
     4921 actggacaag agcaaagtca taaactctgc tctggaatta ctcaatggag tcggtatcga
     4981 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     5041 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     5101 tcatacccac tcctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     5161 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     5221 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     5281 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     5341 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     5401 cgattctatg cccccacttc tgaaacaagc aattgagctg ttcgaccggc agggagccga
     5461 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     5521 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     5581 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     5641 tgacatgctc cccgggggaa gcggagctac taacttcagc ctgctgaagc aggctggaga
     5701 cgtggaggag aaccctggac ctaccgagta caagcccacg gtgcgcctcg ccacccgcga
     5761 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     5821 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     5881 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     5941 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     6001 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6061 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6121 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6181 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     6241 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     6301 ccgcaagccc ggtgcctaac tgtttcgcct cggcgtccgg actagaggta cctttaagac
     6361 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6421 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6481 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6541 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6601 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     6661 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     6721 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6781 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     6841 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     6901 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6961 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7081 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7141 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7201 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7261 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7321 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7381 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7441 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7501 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7561 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7621 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7681 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7741 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7801 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7861 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7921 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7981 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8041 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8101 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8161 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8221 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8281 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8341 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8401 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8461 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     8521 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     8581 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     8641 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8701 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8761 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8821 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8881 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8941 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9001 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9061 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9121 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagc
//
