LOCUS       Copy_of_pPfetFn-Target_ 6365 bp ds-DNA     circular     30-JAN-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             complement(81..584)
                     /label="orf2 CDS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             908..1531
                     /label="catP CDS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(1617..2178)
                     /label="p15A rep_origin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1689..1705
                     /label="p15a-F"
                     /note="sequence: gggcggtttttcgaagg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2419..2440
                     /label="Seq-traJ-F"
                     /note="sequence: gccgggcaggataggtgaagta"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             2495..2935
                     /label="traJ CDS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3081..3086
                     /label=-35
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    3104..3109
                     /label=-10
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3113..3113
                     /label="TSS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3135..3141
                     /label="RBS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    3152..4573
                     /label="TTG_SacB"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3415..3436
                     /label="Seq-SacB-F"
                     /note="sequence: gccattacaaaacgctgacggc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          4469..4490
                     /label="Seq-pTargetv2-F"
                     /note="sequence: caatcaacgtttgcgccaagct"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(4581..4602)
                     /label="Ttyrs-R"
                     /note="sequence: acacgaagggacgattgattat"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4581..4625
                     /label="Terminator tyrS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          4626..4644
                     /label="M13-R"
                     /note="sequence: caggaaacagctatgaccg"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4674..4688
                     /label="tetO"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_fwdcolor="#008080"
     misc_feature    4702..4702
                     /label="TSS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4703..4721
                     /label="FncrRNA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4739..4757
                     /label="FncrRNA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             4764..4802
                     /label="lacZ alpha CDS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4780..4797)
                     /label="M13-F"
                     /note="sequence: gtaaaacgacggccagtg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     terminator      4804..4845
                     /label="Cpa fdx terminator terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             5300..6355
                     /label="repA CDS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
ORIGIN
        1 tactttatat atatatgatt aaaaaaataa aaaacaacag cctattaggt tgttgttttt
       61 tattttcttt attaattttt ttaattttta gtttttagtt cttttttaaa ataagtttca
      121 gcctcttttt caatattttt taaagaagga gtatttgcat gaattgcctt ttttctaaca
      181 gacttaggaa atattttaac agtatcttct tgcgccggtg attttggaac ttcataactt
      241 actaatttat aattattatt ttctttttta attgtaacag ttgcaaaaga agctgaacct
      301 gttccttcaa ctagtttatc atcttcaata taatattctt gacctatata gtataaatat
      361 atttttatta tatttttact tttttctgaa tctattattt tataatcata aaaagtttta
      421 ccaccaaaag aaggttgtac tccttctggt ccaacatatt tttttactat attatctaaa
      481 taatttttgg gaactggtgt tgtaatttga ttaatcgaac aaccagttat acttaaagga
      541 attataacta taaaaatata taggattatc tttttaaatt tcattattgg cctccttttt
      601 attaaattta tgttaccata aaaaggacat aacgggaata tgtagaatat ttttaatgta
      661 gacaaaattt tacataaata taaagaaagg aagtgtttgt ttaaatttta tagcaaacta
      721 tcaaaaatta gggggataaa aatttatgaa aaaaaggttt tcgatgttat ttttatgttt
      781 aactttaata gtttgtggtt tatttacaaa ttcggccggc cagtgggcaa gttgaaaaat
      841 tcacaaaaat gtggtataat atctttgttc attagagcga taaacttgaa tttgagaggg
      901 aacttagatg gtatttgaaa aaattgataa aaatagttgg aacagaaaag agtattttga
      961 ccactacttt gcaagtgtac cttgtaccta cagcatgacc gttaaagtgg atatcacaca
     1021 aataaaggaa aagggaatga aactatatcc tgcaatgctt tattatattg caatgattgt
     1081 aaaccgccat tcagagttta ggacggcaat caatcaagat ggtgaattgg ggatatatga
     1141 tgagatgata ccaagctata caatatttca caatgatact gaaacatttt ccagcctttg
     1201 gactgagtgt aagtctgact ttaaatcatt tttagcagat tatgaaagtg atacgcaacg
     1261 gtatggaaac aatcatagaa tggaaggaaa gccaaatgct ccggaaaaca tttttaatgt
     1321 atctatgata ccgtggtcaa ccttcgatgg ctttaatctg aatttgcaga aaggatatga
     1381 ttatttgatt cctattttta ctatggggaa atattataaa gaagataaca aaattatact
     1441 tcctttggca attcaagttc atcacgcagt atgtgacgga tttcacattt gccgttttgt
     1501 aaacgaattg caggaattga taaatagtta acttcaggtt tgtctgtaac taaaaacaag
     1561 tatttaagca aaaacatcgt agaaatacgg tgttttttgt taccctaagt ttaaacaaga
     1621 tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc
     1681 gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac
     1741 tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat
     1801 gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc
     1861 atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg
     1921 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
     1981 caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     2041 caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat ctttatagtc
     2101 ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
     2161 ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa gtatcttcct
     2221 ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac
     2281 gaccgagcgt agcgagtggg ccccctgctt cggggtcatt atagcgattt tttcggtata
     2341 tccatccttt ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg
     2401 gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg
     2461 ttccttcttc actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg
     2521 aggctggccg gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa
     2581 gccaaccagg aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc
     2641 gattgaggaa aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg
     2701 ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat
     2761 caatggcgac ctgggccgcc tgggcggcct gctgaaactc tggctcaccg acgacccgcg
     2821 cacggcgcgg ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg aagagaagca
     2881 ggacgagctt ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc catgactttt
     2941 ttagccgcta aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca
     3001 tcaagaagag cgacttcgcg gagctggtga agtacatcac cgacgagcaa ggcaagaccg
     3061 atcgTTTTTA ACAAAATATA TTGATAAAAA TAATAATAGT GGGTATAATT AACGTATAAA
     3121 GTTGTGTAAT TTTTAAGGAG GTGTGTTACA TTtgaacatc aaaaagtttg caaaacaagc
     3181 aacagtatta acctttacta ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa
     3241 agaaacgaac caaaagccat ataaggaaac atacggcatt tcccatatta cacgccatga
     3301 tatgctgcaa atccctgaac agcaaaaaaa tgaaaaatat caagttcctg aattcgattc
     3361 gtccacaatt aaaaatatct cttctgcaaa aggcctggac gtttgggaca gctggccatt
     3421 acaaaacgct gacggcactg tcgcaaacta tcacggctac cacatcgtct ttgcattagc
     3481 cggagatcct aaaaatgcgg atgacacatc gatttacatg ttctatcaaa aagtcggcga
     3541 aacttctatt gacagctgga aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga
     3601 tgcaaatgat tctatcctaa aagaccaaac acaagaatgg tcaggttcag ccacatttac
     3661 atctgacgga aaaatccgtt tattctacac tgatttctcc ggtaaacatt acggcaaaca
     3721 aacactgaca actgcacaag ttaacgtatc agcatcagac agctctttga acatcaacgg
     3781 tgtagaggat tataaatcaa tctttgacgg tgacggaaaa acgtatcaaa atgtacagca
     3841 gttcatcgat gaaggcaact acagctcagg cgacaaccat acgctgagag atcctcacta
     3901 cgtagaagat aaaggccaca aatacttagt atttgaagca aacactggaa ctgaagatgg
     3961 ctaccaaggc gaagaatctt tatttaacaa agcatactat ggcaaaagca catcattctt
     4021 ccgtcaagaa agtcaaaaac ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa
     4081 cggcgctctc ggtatgattg agctaaacga tgattacaca ctgaaaaaag tgatgaaacc
     4141 gctgattgca tctaacacag taacagatga aattgaacgc gcgaacgtct ttaaaatgaa
     4201 cggcaaatgg tacctgttca ctgactcccg cggatcaaaa atgacgattg acggcattac
     4261 gtctaacgat atttacatgc ttggttatgt ttctaattct ttaactggcc catacaagcc
     4321 gctgaacaaa actggccttg tgttaaaaat ggatcttgat cctaacgatg taacctttac
     4381 ttactcacac ttcgctgtac ctcaagcgaa aggaaacaat gtcgtgatta caagctatat
     4441 gacaaacaga ggattctacg cagacaaaca atcaacgttt gcgccaagct tcctgctgaa
     4501 catcaaaggc aagaaaacat ctgttgtcaa agacagcatc cttgaacaag gacaattaac
     4561 agttaacaaa taactgcagg ataatcaatc gtcccttcgt gtaaacgaag gggcgttttt
     4621 tatttcagga aacagctatg accgcggccg ctgtatccaA AATTACTTTA AAATCTATCA
     4681 TTGATAGGGT AAAATATAAA TCAATTTCTA CTGTTGTAGA TtGAGACGat tCGTCTCcAA
     4741 TTTCTACTGT TGTAGATttt tttagacatg caagcttggc actggccgtc gttttacact
     4801 agcataaaaa taagaagcct gcatttgcag gcttcttatt tttatggcgc gACGTCgttc
     4861 tgaatcctta gctaatggGT CGACgtatat aaaatgtgct aatgaaaaag aaaatgcgtt
     4921 aaaagagcct aaaatgagtt caaatggttt tgaaattgat tggtagttta atttaatata
     4981 ttttttctat tggctatctc gatacctata gaatcttctg ttcacttttg tttttgaaat
     5041 ataaaaaggg gctttttagc cccttttttt taaaactccg gaggagtttc ttcattcttg
     5101 atactatacg taactatttt cgatttgact tcattgtcaa ttaagctagt aaaatcaatg
     5161 gttaaaaaac aaaaaacttg catttttcta cctagtaatt tataatttta agtgtcgagt
     5221 ttaaaagtat aatttaccag gaaaggagca agttttttaa taaggaaaaa tttttccttt
     5281 taaaattcta tttcgttata tgactaatta taatcaaaaa aatgaaaata aacaagaggt
     5341 aaaaactgct ttagagaaat gtactgataa aaaaagaaaa aatcctagat ttacgtcata
     5401 catagcacct ttaactacta agaaaaatat tgaaaggact tccacttgtg gagattattt
     5461 gtttatgttg agtgatgcag acttagaaca ttttaaatta cataaaggta atttttgcgg
     5521 taatagattt tgtccaatgt gtagttggcg acttgcttgt aaggatagtt tagaaatatc
     5581 tattcttatg gagcatttaa gaaaagaaga aaataaagag tttatatttt taactcttac
     5641 aactccaaat gtaaaaagtt atgatcttaa ttattctatt aaacaatata ataaatcttt
     5701 taaaaaatta atggagcgta aggaagttaa ggatataact aaaggttata taagaaaatt
     5761 agaagtaact taccaaaagg aaaaatacat aacaaaggat ttatggaaaa taaaaaaaga
     5821 ttattatcaa aaaaaaggac ttgaaattgg tgatttagaa cctaattttg atacttataa
     5881 tcctcatttt catgtagtta ttgcagttaa taaaagttat tttacagata aaaattatta
     5941 tataaatcga gaaagatggt tggaattatg gaagtttgct actaaggatg attctataac
     6001 tcaagttgat gttagaaaag caaaaattaa tgattataaa gaggtttacg aacttgcgaa
     6061 atattcagct aaagacactg attatttaat atcgaggcca gtatttgaaa ttttttataa
     6121 agcattaaaa ggcaagcagg tattagtttt tagtggattt tttaaagatg cacacaaatt
     6181 gtacaagcaa ggaaaacttg atgtttataa aaagaaagat gaaattaaat atgtctatat
     6241 agtttattat aattggtgca aaaaacaata tgaaaaaact agaataaggg aacttacgga
     6301 agatgaaaaa gaagaattaa atcaagattt aatagatgaa atagaaatag attaaagtgt
     6361 aacta
//