LOCUS       Copy_of_*(YS515)pAAV-CM 7242 bp ds-DNA     circular     07-MAR-2023
DEFINITION  .
ACCESSION   urn.local...14-4c2f3f0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
FEATURES             Location/Qualifiers
     misc_feature    1..858
                     /label="misc_feature"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(8..25)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     184..201
                     /label="primer_bind"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(187..204)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     627..644
                     /label="primer_bind"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(633..650)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      1009..1676
                     /label="rep_origin"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     1076..1093
                     /label="primer_bind"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(1098..1115)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1377..1396)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1521..1540
                     /label="YS741"
                     /note="sequence: gggaaacgcctggtatcttt"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     1542..1559
                     /label="primer_bind"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(1544..1561)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     1634..1653
                     /label="primer_bind"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1653..1672
                     /label="YS725"
                     /note="sequence: ggccttttgctggccttttg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1682..1822
                     /label="5'-ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1811..1854
                     /label="YS870"
                     /note="sequence: ctaggggttcctgcggccgcacgcgttgacattgattattgact"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     enhancer        1838..2217
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2060..2079)
                     /label="YS740"
                     /note="sequence: ttgatgtactgccaagtggg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        2218..2421
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     2372..2392
                     /label="CMV-F"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2403..2420
                     /label="3CMVFW(Sequetech)"
                     /note="sequence: GGTCTATATAAGCAGAGC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          2423..2443
                     /label="YS43"
                     /note="sequence: TCTGGCTAACTAGAGAACCCA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        2466..2484
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          2466..2484
                     /label="pT7 (Sequetech)"
                     /note="sequence: TAATACGACTCACTATAGG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     2466..2485
                     /label="T7"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2466..2485
                     /label="YS592"
                     /note="sequence: taatacgactcactataggg"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2479..2528
                     /label="YS783"
                     /note="sequence: tatagggagacccaagctgGCTAGCGTTTAAACTTAAGCTTGCCACCATG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2495..2544
                     /label="YS372"
                     /note="sequence: ctggctagcgtttaaacttaagcttgccaccATGGTGAGCAAGGGCGAGG"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          2526..2544
                     /label="YS359"
                     /note="sequence: ATGGTGAGCAAGGGCGAGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2526..3926
                     /label="tdTomato (-C9)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2526..4637
                     /label="ERK tKiMBImut"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             2526..5891
                     /label="Translation 2526-5891"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHLFRLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTINSIMADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVTGYRLFEPDAPRAPVDKAKLSFQFPGSGEGRGSLLTCGDVEENPGPGSGMPKKKPTPIQLNPTPDGSAVNGTSSAETNLEALQKKLEGLELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDDMANDFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLLVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAASI*"
     primer          2529..2546
                     /label="YS296"
                     /note="sequence: gtgagcaagggcgaggag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2719..2739)
                     /label="YS242"
                     /note="sequence: ccttggagccgtacatgaact"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(3183..3200)
                     /label="primer oYW357 extra binding site"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3238..3255
                     /label="oYW369_tdTomato(mid)_F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(3244..3263)
                     /label="oYW368_tdTomato(mid)_R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(3445..3465)
                     /label="YS242"
                     /note="sequence: ccttggagccgtacatgaact"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(3909..3943)
                     /label="oYW357_tdTomato_R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3920..3957
                     /label="oYW358_lgbit(-N5)_F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    3927..3932
                     /label="RL linker"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3933..4394
                     /label="LgBiT(-n5)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          3943..3963
                     /label="YS398"
                     /note="sequence: TTGGGGACTGGGAACAGACAG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(4085..4106)
                     /label="oYW359_seq_lgbit_R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4091..4115)
                     /label="YS831"
                     /note="sequence: accttcatacgggatgatgacatgg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(4295..4316)
                     /label="YS829"
                     /note="sequence: cagggtccctgttacagtgatc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(4368..4391)
                     /label="YS299"
                     /note="sequence: GTTGATGGTTACTCGGAACAGCAT"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(4371..4418)
                     /label="YS693"
                     /note="sequence: cagcttctcctcgtccgccatgatGCTGTTGATGGTTACTCGGAACAG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(4372..4394)
                     /label="YS827"
                     /note="sequence: GCTGTTGATGGTTACTCGGAACA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          4398..4416
                     /label="YS675"
                     /note="sequence: atggcggacgaggagaagc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    4398..4559
                     /label="template from YS366"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4413..4559
                     /label="WW domain"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(4421..4436)
                     /label="YS788"
                     /note="sequence: cttctcccagccgggc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(4450..4469)
                     /label="YS779"
                     /note="sequence: gtagtacactcggcctgagc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(4544..4559)
                     /label="YS718"
                     /note="sequence: cctggcaggctccccc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(4544..4583)
                     /label="YS704"
                     /note="sequence: CTCGAACAGCCGGTAGCCGGTCACcctggcaggctccccc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          4560..4576
                     /label="YS846"
                     /note="sequence: GTGACCGGCTACCGGCT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4560..4583
                     /label="SmBiT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4584..4637
                     /label="ERKsub(S-3A,T0A)"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          4602..4624
                     /label="YS814"
                     /note="sequence: CCAGTGGATAAAGCAAAGCTGTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4607..4663)
                     /label="YS812"
                     /note="sequence: AGGGAGCCTCGACCTTCACCGGATCCGGGGAATTGGAATGACAGCTTTGCTTTATCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(4615..4637)
                     /label="YS798"
                     /note="sequence: GGGGAATTGGAATGACAGCTTTG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(4615..4674)
                     /label="YS790"
                     /note="sequence: CACAAGTGAGGAGGGAGCCTCGACCTTCACCGGATCCGGGGAATTGGAATGACAGCTTTG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4638..4700
                     /label="T2A"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(4682..4700)
                     /label="YS899"
                     /note="sequence: AGGCCCTGGGTTTTCTTCC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          4682..4731
                     /label="YS900"
                     /note="sequence: GGAAGAAAACCCAGGGCCTggctcaggtATGcccaagaagaagccgacgc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     cds2            4713..4889
                     /label="MEK(Nterm)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     cds             4713..5888
                     /label="MEK1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4737..4742
                     /label="PvuII"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     mutation        4824..4826
                     /label="E to G"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    4825..4825
                     /label="A"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     mutation        4836..4838
                     /label="E to D"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4838..4838
                     /label="G"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4850..4850
                     /label="A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     mutation        4851..4853
                     /label="N to K"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    4853..4853
                     /label="C"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4856..4856
                     /label="T"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4859..4859
                     /label="G"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4967..4981
                     /label="XcmI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    5044..5050
                     /label="PfoI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(5054..5060)
                     /label="AarI"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    5123..5132
                     /label="BsaBI, Bse8I, BseJI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(5177..5203)
                     /label="P566-R-EcoR1-MEK1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5188..5193
                     /label="EcoRI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     mutation        5361..5363
                     /label="218D"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     mutation        5373..5375
                     /label="222D"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    5395..5400
                     /label="PciI, PscI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5512..5523
                     /label="BstXI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    5585..5591
                     /label="PasI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(5870..5921)
                     /label="YS901"
                     /note="sequence: cgccactgtgctggatatctgcagaattcattagatgctagcggcatgggtt"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    5880..5885
                     /label="NheI, AsuNHI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5880..5885
                     /label="BmtI, BspOI"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(5893..5917)
                     /label="YS418"
                     /note="sequence: actgtgctggatatctgcagaattc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    5894..5908
                     /label="in-fusion"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(5900..5921)
                     /label="oYW244"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(5901..5950)
                     /label="YS898"
                     /note="sequence: tagaaggcacagtcgaggcctcgagcggccgccactgtgctggatatctg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(5903..5920)
                     /label="SC64"
                     /note="sequence: gccactgtgctggatatc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(5933..5950)
                     /label="pcDNA3.1BGHRev"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     polyA_signal    5939..6163
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(5957..5977)
                     /label="YS191"
                     /note="sequence: AGGGGCAAACAACAGATGGCT"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(5976..5999)
                     /label="pFLU-DW"
                     /note="sequence: AGGGTCAAGGAAGGCACGGGGGAG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(6145..6187)
                     /label="YS894"
                     /note="sequence: ctgcggccgctcggtccgcacgtgccatagagcccaccgcatc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6186..6326
                     /label="3'-ITR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      6418..6724
                     /label="rep_origin"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     6500..6517
                     /label="primer_bind"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(6544..6561)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(6919..6936)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     6992..7014
                     /label="primer_bind"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(7045..7061)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(7112..7129)
                     /label="Geneious type: primer_bind_reverse"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
       61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
      481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
      541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
      601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
      901 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
      961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1141 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     1201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     1441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     1501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     1561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     1621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     1681 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt
     1741 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
     1801 aactccatca ctaggggttc ctgcggccgc acgcgttgac attgattatt gactagttat
     1861 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     1921 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     1981 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2041 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2101 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2161 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2221 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2281 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2341 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2401 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
     2461 gaaattaata cgactcacta tagggagacc caagctgGCT AGCGTTTAAA CTTAAGCTTG
     2521 CCACcatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca
     2581 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     2641 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     2701 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     2761 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     2821 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     2881 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     2941 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     3001 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     3061 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     3121 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     3181 agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg
     3241 gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg
     3301 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     3361 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     3421 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
     3481 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     3541 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
     3601 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
     3661 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     3721 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac tacctggtgg
     3781 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
     3841 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     3901 gctccgaggg ccgccaccac ctgttccggc tgGAAGATTT CGTTGGGGAC TGGGAACAGA
     3961 CAGCCGCCTA CAACCTGGAC CAAGTCCTTG AACAGGGAGG TGTGTCCAGT TTGCTGCAGA
     4021 ATCTCGCCGT GTCCGTAACT CCGATCCAAA GGATTGTCCG GAGCGGTGAA AATGCCCTGA
     4081 AGATCGACAT CCATGTCATC ATCCCGTATG AAGGTCTGAG CGCCGACCAA ATGGCCCAGA
     4141 TCGAAGAGGT GTTTAAGGTG GTGTACCCTG TGGATGATCA TCACTTTAAG GTGATCCTGC
     4201 CCTATGGCAC ACTGGTAATC GACGGGGTTA CGCCGAACAT GCTGAACTAT TTCGGACGGC
     4261 CGTATGAAGG CATCGCCGTG TTCGACGGCA AAAAGATCAC TGTAACAGGG ACCCTGTGGA
     4321 ACGGCAACAA AATTATCGAC GAGCGCCTGA TCACCCCCGA CGGCTCCATG CTGTTCCGAG
     4381 TAACCATCAA CAGCatcatg gcggacgagg agaagctgcc gcccggctgg gagaagcgca
     4441 tgagccgcag ctcaggccga gtgtactact tcaaccacat cactaacgcc agccagtggg
     4501 agcggcccag cggcaacagc agcagtggtg gcaaaaacgg gcagggggag cctgccaggG
     4561 TGACCGGCTA CCGGCTGTTC GAGCCAGATg cCCCTAGAgc cCCAGTGGAT AAAGCAAAGC
     4621 TGTCATTCCA ATTCCCCGGA TCCGGTGAAG GTCGAGGCTC CCTCCTCACT TGTGGGGATG
     4681 TGGAAGAAAA CCCAGGGCCT ggctcaggtA TGcccaagaa gaagccgacg cccatccagc
     4741 tgaacccgac ccccgatggc tcggcggtta acgggaccag ctcggccgag accaacctgg
     4801 aggccttgca gaagaagctg gaggggctgg agcttgatga gcagcagcgg aagcgccttg
     4861 aggcctttct gacccagaag cagaaggtgg gagaactgaa ggatgatgac tttgagaaga
     4921 tcagtgagct gggggccggc aacggtggag tggtattcaa ggtctcccat aagccgtctg
     4981 gcctagttat ggccaggaag ctaattcatc tggagatcaa acccgcaatc cggaaccaga
     5041 tcatccggga gctgcaggtg ctgcatgagt gcaactcccc atacatcgtg ggcttctatg
     5101 gggctttcta cagtgacggc gagatcagca tctgcatgga acacatggat ggtgggtcct
     5161 tggatcaagt tctgaagaaa gctggaagaa ttcctgagca aattttagga aaagttagca
     5221 ttgctgtgat aaaaggcctg acatatcttc gggagaagca caagattatg cacagagatg
     5281 tcaagccttc caatattcta gtaaactccc gtggagaaat caaactctgt gattttgggg
     5341 tcagcgggca actaattgac gacatggcca acgactttgt aggcacaaga tcatacatgt
     5401 cgcccgagag actccagggg actcactact ccgtgcagtc ggacatctgg agcatggggc
     5461 tctctctggt ggagatggca gttgggagat accccatccc tcctcctgat gccaaggaac
     5521 tggagctgct gtttggatgc caggtggagg gagatgcagc cgaaacgcca cccaggccga
     5581 ggacccctgg gaggcccctt agctcatatg gaatggacag ccgacctccc atggcaattt
     5641 ttgagttgtt ggattatata gtcaatgagc ctcctccaaa attgcccagt ggagtattca
     5701 gtctggagtt tcaagatttt gtgaataaat gcttaataaa gaaccctgca gagagagcag
     5761 atctaaagca gcttctggtt cacgctttca tcaagagatc tgatgccgag gaagtagact
     5821 tcgcaggttg gctctgctcc accattgggc ttaaccagcc cagcacacca acccatgccg
     5881 ctagcatcta atgaattctg cagatatcca gcacagtggc ggccgctcga ggcctcgact
     5941 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6001 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6061 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6121 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcacgtgc ggaccgagcg
     6181 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     6241 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     6301 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
     6361 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     6421 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6481 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6541 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6601 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6661 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6721 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     6781 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6841 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     6901 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6961 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     7021 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7081 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7141 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7201 taaccctgat aaatgcttca ataatattga aaaaggaaga gt
//